• Journal of Microbiology and Biotechnology
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• 제21권10호
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• pp.1012-1020
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• 2011

A gene coding for an endoglucanase (EglA), of the glycosyl hydrolase family 12 and derived from Aspergillus niger VTCC-F021, was cloned and sequenced. The cDNA sequence, 717 bp, and its putative endoglucanase, a 238 aa protein with a predicted molecular mass of 26 kDa and a pI of 4.35, exhibited 98.3-98.7% and 98.3-98.6% identities, respectively, with cDNA sequences and their corresponding endoglucanases from Aspergillus niger strains from the GenBank. The cDNA was overexpressed in Pichia pastoris GS115 under the control of an AOX1 promoter with a level of 1.59 U/ml culture supernatant, after 72 h of growth in a YP medium induced with 1% (v/v) of methanol. The molecular mass of the purified EglA, determined by SDS-PAGE, was 33 kDa, with a specific activity of 100.16 and 19.91 U/mg toward 1% (w/v) of ${\beta}$-glucan and CMC, respectively. Optimal enzymatic activity was noted at a temperature of $55^{\circ}C$ and a pH of 5. The recombinant EglA (rEglA) was stable over a temperature range of $30-37^{\circ}C$ and at pH range of 3.5-4.5. Metal ions, detergents, and solvents tested indicated a slightly inhibitory effect on rEglA activity. Kinetic constants ($K_m$, $V_{max}$, $k_{cat}$, and $k_{cat}/K_m$) determined for rEglA with ${\beta}$-glucan as a substrate were 4.04 mg/ml, 102.04 U/mg, 2,040.82 $min^{-1}$, and 505.05, whereas they were 10.17 mg/ml, 28.99 U/mg, 571.71 $min^{-1}$, and 57.01 with CMC as a substrate, respectively. The results thus indicate that the rEglA obtained in this study is highly specific toward ${\beta}$-glucan. The biochemical properties of rEglA make it highly valuable for downstream biotechnological applications, including potential use as a feed enzyme.

• 대한의용생체공학회:의공학회지
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• 제38권2호
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• pp.62-73
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• 2017

To identify a solution for the restricted availability of healthy lungs and the high risk of immune rejections following organ transplantation, tissue engineering techniques for culturing lungs have been studied by many research groups. The most promising method for culturing lungs is the utilization of a bio-scaffold that was prepared using harvested organs from human donors or other animals by removing their original cells. In this study, a pulsatile perfusion pump was used to alleviate the cell removal effect with the high fluid-dynamic power of the perfusion stream during the decellularization process, while other conventional studies focused on chemical methods to identify efficient detergents. The purpose of this study was to analyze the developed device by using energy equivalent pressure (EEP), which is an indicator of pulsatility, to understand the characteristics of pulsatile energy transmitted according to the load size by using the artificial model and compare it with the measured EEP. The pulsatility of the device can be estimated with the concept of fluid-dynamic energy during a particular constant time period or fluid-dynamic power represented as EEP and EEP increment. Because the measured EEP of perfusion flow during decellularization can be changed by the amount of fluid leakage and the degree of clogging in the capillary vessels, EEP should be measured to determine whether the decellularization is progressing without problems. The decrement of EEP caused by the high perfusion resistance was observed from some experimental results that were obtained with artificial models. EEP can be used to monitor the decellularization process after analyzing the varying EEP according to the amount of load. It was confirmed that the EEP was maintained at a high level in the experiment using the harvested lungs from 12-13-week-old rats. In addition, it was confirmed that the cell removal time was faster than when continuous perfusion was performed. In this study, pulsatile power delivered to the lungs was measured to monitor the process of cell removal, and it serve as the evidence for efficient decellularization.

• 한국산업보건학회지
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• 제16권3호
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• pp.233-244
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• 2006

Of many volatile organic detergents for metals, benzine(CAS No. 8030-30-6), of which the toxicity has not yet been proven, has been used as an alternative of the halide compounds in the consideration of toxic effects, global warming and the destruction of ozone layer. In order to evaluate the effects of the benzine on human body by investigating the subchronic inhalation toxicity, to obtain the basic data for establishing the criteria of exposure in working environments and to classify the hazardousness in compliance with the Industrial Safety and Health Act by evaluating the hazardousness, repeated inhalation exposure test was carried with SD rats. The rats were grouped by 10 females and males each. The repetitive inhalation exposures were carried out at 4 levels of concentration of 0 ppm, 60 ppm, 300 ppm, and 1,500 ppm, for 6 hours a day, 5 days a week, for 13 weeks. The results are described hereunder. 1. No death of the animals of the exposed and controlled groups in the test period. Not any specific clinical symptoms, change in feed intake quantity, abnormality in eye test, or change in activity were observed. 2. In the 300 ppm and 1,500 ppm groups, weight reduction in the female groups and weight increase of liver and kidney in the male groups compared with control group were observed with statistical significance(p<0.05). 3. In the blood test, the HCT increased in the male 300 ppm group and the number of hematocyte increased, MCV and MCH decreased in the male 1,500 ppm group. In the female 1,500 ppm group, the HB decreased and the distribution width of the hematocyte particle size increased. In the blood biochemistry test, the TP in the male 1,500 ppm group and the LDH in the female 1,500 ppm group were increased with statistical significance(p<0.05). 4. Under the test conditions of the present study with SD rats, the NOEL was evaluated to be from 60 ppm to 300 ppm for both male and female groups. By extrapolation, the NOEL for human who work 8 hours a day was evaluated to be from 128 ppm to 640 ppm 5. Since the NOEL evaluated in this study do not exceed 60ppm(0.184 mg/L) the test material does not belong to the classification of the hazardous substance "NOEL${\leq}$0.5mg/L/6hr/90day(rat), for continuous inhalation of 6hours a day for 90 days" nor to the basic hazardous chemical substance class 1(0.2 mg/L/6hr/90day(rat) defined by the GHS which is a criteria of classification and identification of chemical compounds. However, considering the boiling point($30-204^{\circ}C$), flashing point($-40^{\circ}C$), vapor pressure(40 mmHg), and the inflammable range(1.0 - 6.0 %), sufficient care should be taken for handling in the safety aspects including fire or explosion.

• Toxicological Research
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• 제20권3호
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• pp.195-203
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• 2004

4-Tert-Octylphenol (OP) is a surfactant additive widely used in the manufacture of a variety of detergents and plastic products. OP can disrupt endocrine function in humans and animals. This study was carried out to obtain toxicokinetic parameters of OP in male Sprague-Dawley (SD) rats. Male rats were administered with OP by single oral application of 200 mg/kg body weight. Blood, urine and tissues samples were taken at several time intervals after administration. Analysis of samples for OP was performed by column-switching high performance liquid chromatography (HPLC). In addition, we exam-ined tissue distribution and accumulation of OP after single oral application of 50, 100, and 200 mg/kg, single intravenous injection of 1, 5 and 10 mg/kg or daily application of 50 mg/kg for 14 consecutive days. After single oral administration of 200 mg/kg, Cmax of 213 $\pm$ 123 ng/ml was reached within the first 1.3 hr (Tmax) in the plasma. AUC was calculated for 1,333$\pm$484 ngㆍhr/ml. The final elimination half-life of plasma was longer than that of urine, but urinary clearance was lower than oral. A very small fraction of OP (Fe < 0.0017%) was excreted in urine within 24 hr. These results indicated that the major excretion route of OP was not urine. The mean maximal tissue distribution of OP was obserbed at 6 hr after treatment and slowly decreased time-dependently. High OP concentrations were detected in fat at 24 hr. The OP in fat was slowly released with longer elimination half-life and lower clearance than that of other tissues. OP was not accumulated in the liver following single oral application but 14-day oral treatments resulted in two-fold accumulation. It was probably due to the saturation of detoxification pathways. On the other hand, the mRNA expression of cytochrome P450 isoforms except CYP2C11 was not affected by OP at any dose. The expression of CYP2C11 mRNA decreased in a dose-dependent manner. This result suggests that OP changes expression of the male-specific cytochrome P450 isoforms in rat liver, and these changes are closely related to the toxic and estrogenic effect of OP.

• 한국화재소방학회논문지
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• 제33권3호
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• pp.51-55
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• 2019

CFC의 오존층 파괴로 인해 이를 대체할 냉매, 세정제, 발포제의 개발과 더불어 소방분야에서는 할론대체소화약제개발에 주력하여 왔다. 특히 소화기분야에서는 할론1211 소화기를 대체할 청정소화약제를 개발하여 왔다. 그 결과로 개발된 소화기용 청정소화약제 중 가장 널리 사용되는 소화약제가 HCFC-123이다. 대부분의 청정소화약제들은 자체 증기압이 약하기 때문에 어떠한 가압원을 사용할 것인가가 중요한 문제 중 하나이다. 본 연구에서는 소화기용 소화약제들 중 HCFC-123, HCFC-124, HFC-125, Novec-1230을 선정하여 이들 약제에 대한 가압원으로 보조소화효과를 기대할 수 있는 CO₂를 사용하였다. 각 소화약제별로 약제량과 CO₂량을 조절하며 시험을 실시한 결과 HCFC-123 소화약제로부터 기대한 소화효과를 확인할 수 있었다. 현재 시중에 판매되고 있는 소화기인 HCFC-123 2.5 kg을 질소로 가압하여 소화능력 ABC 각 1단위인 소화기보다 소화약제가 적은 HCFC-123 1.5 kg과 CO₂ 1.5 kg을 혼합한 소화기로 동일한 소화능력시험에 성공하였다. 이러한 소화시험의 결과는 가압원인 CO₂의 보조소화효과를 확인한 것이라 할 수 있다. 이는 중간대체물질로 분류되어 있는 HCFC계열의 소화약제를 줄일 수 있어 기존의 소화기보다 친환경적이고 경제적이라 할 수 있으며 B,C급 화재용 소화기인 CO₂ 소화기로 A급 가연물이 많은 전기, 전자 관련 시설을 방호하는 불합리함을 해결하는데 기여할 수 있으리라 기대한다.

• 대한수의학회지
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• 제44권1호
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• pp.29-40
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• 2004

Because cleaning products are part of our everyday lives, it is essential that they should not present significant risks to health. However, many petrochemicals in most soaps and detergents can be absorbed through the scalp and skin and, over time, accumulate in the organs and tissues. This accumulation may result in brain, nerve, and liver damage. Therefore, it is interested in developing non-harmful detergent. According to Korea Research Institute of Chemical Technology, GC-100X may be non-harmful and non-corrosive alkaline ionic water (pH 12). It is composed of hydroxyl radicals and supplemented with xylitol. To evaluate influence of GC-100X on rats and beagles, GC-100X was diluted with distilled water (25%, 50%, and 100% solution respectively). Each of diluted GC-100X was daily treated per oral. In body weight analysis, urinary analysis, ophthalmological test and autopsy, we did not find any significance, but in serum biochemical analysis and hematological analysis, we found some significances in middle dose group compared with control group. These significances in serum biochemical analysis and hematological analysis may be not induced by GC-100X, because it was not found to be significant from control group in histopathological examination. Thus, it is concluded that NOEL(No Observed Effect Level) of GC-100X may be higher than all treatment doses used in this study, and GC-100X may be a non-toxic detergent.

• 대한기계학회논문집B
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• 제38권4호
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• pp.337-346
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• 2014

본 연구는 주기적 강제 진동이 가해지는 표면의 액적 모드 특성과 표면에 놓인 액적의 제거에 대한 조건을 실험적으로 이해하는 것을 목적으로 하고 있다. 액적의 거동을 명확하게 관찰하기 위해 아크릴 표면에 Teflon 코팅을 진행하여 접촉각을 높였고, 히스테리시스는 25도 이내로 진행하여 액적의 거동이 보다 쉽게 진행되도록 하였다. 본 실험은 먼지가 적은 청정실에서 실험이 진행되었다. 제작된 소수성 표면에 놓인 액적의 실제 공진 주파수를 예측하기 위해 이론 및 실험적 해석을 통해 두 접근방법의 타당성을 파악하였으며, 두 개의 초고속카메라를 액적의 상면과 측면에 설치하여 2가지 측면에서 액적의 다양한 형상 변형 특성- 모드 형상, 분리, 미소 액적의 발생, 그리고 좌우 비틀림의 특성을 관찰하였다. 이론 값 비교결과 실제 공진 주파수 값들의 차이가 약 18% 이하로 관찰되었으며, 이러한 차이는 접촉선 마찰, 비선형 벽 고착, 실험의 불확실성 등에 가장 큰 영향을 받는 것으로 판단된다. 사용된 스피커에 상대적으로 낮은 전압을 인가할 경우 액적의 접촉선은 고정된 상태에서 좌우 대칭적인 액적 형상진동이 나타났다. 반면, 높은 전압을 인가할 경우 액적의 접촉선은 비고정된 상태가 되면서 더 활발한 형상 진동이 나타났다. 가진 주파수가 모드 주파수와 일치할 경우에는 액적의 로브 크기가 주변부 주파수 일 때 보다 비교적으로 컸으며, 같은 전압을 인가 할 경우, 표면에 놓인 액적의 미소 액적 발생 및 완전한 제거는 2차 모드에서만 진행되는 것을 실험을 통해 규명하였다.

• KSBB Journal
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• 제27권6호
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• pp.352-360
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• 2012

Bacillus clausii I-52 which produced SDS- and $H_2O_2$-tolerant extracellular alkaline protease (BCAP) was isolated from heavily polluted tidal mud flat of West Sea in Incheon, Korea and stable strain (transformant C5) of B. clausii I-52 harboring another copy of BCAP gene in the chromosome was developed using the chromosome integration vector, pHPS9-fuBCAP. When investigated the production of BCAP using B. clausii transformant C5 through pilot-scale submerged fermentation (500 L) at $37^{\circ}C$ for 30 h with an aeration rate of 1 vvm and agitation rate of 250 rpm, protease yield of approximately 105,700 U/mL was achieved using an optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_4{\cdot}7H_2O$ 0.01%, $FeSO_4{\cdot}7H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). The enzyme stability of BCAP was increased by addition of polyols (10%, v/v) and also, the stabilities of BCAP towards not only the thermal-induced inactivation at $50^{\circ}C$ but also the SDS and $H_2O_2$-induced inactivation at $50^{\circ}C$ were enhanced. Among the polyols examined, the best result was obtained with propylene glycol (10%, v/v). The BCAP supplemented with propylene glycol exhibited extreme stability against not only the detergent components such as ${\alpha}$-orephin sulfonate (AOS) and zeolite but also the commercial detergent preparations. The granulized enzyme of BCAP was prepared with approximately 1,310,000 U/g of granule. Wash performance analysis using EMPA test fabrics revealed that BCAP granule exhibited high efficiency for removal of protein stains in the presence of anionic surfactants as well as bleaching agents. When compared to Savinase 6T$^{(R)}$ and Everlase 6T$^{(R)}$ manufactured by Novozymes, BCAP under this study probably showed similar or higher efficiency for the removal of protein stains. These results suggest that the alkaline protease produced from B. clausii transformant C5 showing high stability against detergents and high wash performance has significant potential and a promising candidate for use as a detergent additive.

• 생명과학회지
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• 제29권12호
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• pp.1408-1414
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• 2019

효소는 세제, 식품, 사료, 의약품 및 의료용 분야 등 산업 전반적인 응용 분야에서 널리 사용되고 있으며, 산업 제품 및 공정에서 주요 요인이다. 효소를 선별, 확인, 및 특성 분석을 위해 zymography 기술이 일상적으로 사용됩니다. Zymography 기술은 SDS-전기영동을 통해 단백질을 분리한 후 포함된 기질을 겔 상에서 분해하는 기능성 효소를 검출하는 데 널리 사용되는 단순하고 민감하며 정량화가 가능한 기술이다. 이 방법은 비 환원 조건하에서 SDS-전기영동 겔에서 전기영동에 의한 단백질의 분리와 겔 상에서 효소 활성을 검출하는 다목적 2 단계의 기술이다. 이는 SDS-전기영동 겔에 기질을 중합시키고 전기영동 분리 후 효소 반응 완충용액에서 복원된 가수분해 효소에 의해 분해되는 것을 기반으로 하는 기술이다. 미생물 배양액, 식물 추출물, 혈액, 조직 배양액, 식품 속 효소 및 메타 프로테옴을 포함한 어떤 종류의 생물학적 시료들을 zymography에 적용하고 분석이 가능하다. Zymography의 장점은 전처리 없이 혼합된 시료를 적용하여 SDS-전기영동 겔 상에서 활성을 지닌 단백질을 직접 육안으로 검출이 가능할 뿐만 아니라 나노그람(nanogram) 수준에서 활성을 확인이 가능하다. 그래서 이 zymography 기술은 다양한 분야에 응용이 가능하다. 하지만, 이러한 장점이 오히려 단점으로 작용하여 실험적 오류를 범할 수 있는 경우가 많다. 본 총설에서 zymography 기술의 장점, 단점, 및 문제점 해결에 관해서 서술하였다.

• 한국방재학회 논문집
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• 제9권6호
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• pp.51-56
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• 2009

안전 및 품질 평가시스템은 유해물질 재난을 예방하기 위해 유해물질의 생산 저장 수송 취급에 매우 중요하다. 현재 유해물질은 플라스틱, 가정의 세척제, 비료 또는 석유관련제품으로 우리의 일상생활 어디에서든지 존재하고 있다. 그러나 유해물질은 인간이나 환경에 누출되었을 때 매우 위험한 물질이다. 유해물질은 석유기반 경제시대에 매우 폭넓게 사용되고 있다. 우리나라의 환경부는 전세계적으로 유해물질의 유형이 약 십만개가 넘을 것으로 추산하고 있다. 또한 매년 4백개 이상의 새로운 물질이 개발되고 있다. 따라서 유해물질 관리측면에서 우리는 유해물질 사고로부터 안전한가에 대해 의문이 제기될 수 있다. 1984년에 발생해 6,400명이 넘는 사망자와 3만명 이상의 부상자를 낸 인도 보팔사고는 이러한 우리의 유해물질 안전에 대한 불안을 증폭시키는 대표적인 사례이다. 우리나라는 최근 산업의 고도화로 각종 유해물질 사고가 빈번하게 발생하고 있다. 따라서 낙후된 유해물질 관리시스템을 자기관리가 가능한 안전시스템으로 전환하기 위한 위험평가시스템의 구축이 우리사회에 요구되고 있다. 본 연구는 유해물질 위험평가시스템 구축을 위한 기반을 제공하기 위해 지난 10년동안 유해물질 관련시설에서 발생한 사고사례들을 연구의 대상으로 하여 유해물질 시설사고의 특성을 빈도분석, 교차분석 등의 통계기법을 적용하여 분석하였다. 분석결과 사고시설 유형과 연도, 계절, 발생지역, 사망자 발생간 관계가 있는 것으로 나타났다.