• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.3
• /
• pp.1655-1659
• /
• 2013

Background: HER-2/neu is a proto-oncogene that encodes a transmembrane tyrosine kinase growth factor which is crucial for stimulating growth and cellular motility. Overexpression of HER-2/neu is observed in 10-35% of human breast cancers and is associated with pathogenesis, prognosis as well as response to therapy. Given the imperative role of HER-2/neu overexpression in breast cancer, it is important to determine the magnitude of amplification which may facilitate a better prognosis as well as personalized therapy in affected patients. In this study, we determined HER-2/neu protein expression by immunohistochemistry (IHC) concurrently with HER-2/neu DNA amplification by quantitative real time-polymerase chain reaction (Q-PCR). Materials and Methods: A total of 53 paired tissue samples from breast cancer patients were frozen-sectioned to characterize the tumour and normal tissues. Only tissues with 80% tumour cells were used in this study. For confirmation, Q-PCR was used to determine the HER-2/neu DNA amplification. Results: We found 20/53 (37.7%) of the tumour tissues to be positive for HER-2/neu protein overexpression using IHC. Out of these twenty, only 9/53 (17%) cases were in agreement with the Q-PCR results. The concordance rate between IHC and Q-PCR was 79.3%. Approximately 20.7% of positive IHC cases showed no HER-2/neu gene amplification using Q-PCR. Conclusion: In conclusion, IHC can be used as an initial screening method for detection of the HER-2/neu protein overexpression. Techniques such as Q-PCR should be employed to verify the IHC results for uncertain cases as well as determination of HER-2/neu gene amplification.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.9 no.3
• /
• pp.82-92
• /
• 2006

As beaches throughout Korea have suffered great losses of sand due to artificial developments and meteorological phenomena, particularly typhoons, it is necessary to monitor beaches that are prone to erosion continuously, establish and enforce a comprehensive plan to attack coastal erosion with the object of the long-term management. However, debates and temporary measures, not based on accurate coastal zone surveys and analyses, have been established up to now. Therefore, with Haeundae sand beach as a case study, we proposed methods to collect accurate spatial data of the coastline and the sand beach through GPS survey. And we detected and analyzed topographic changes resulting from Typhoon Nabi quantitatively and qualitatively, by using GIS technique. Results showed a mean elevation of 1.95 m, a total area of 53,441 $m^2$, and a total volume of 104,639 $m^3$ after Typhoon Nabi. Mean elevation rose 0.06 m between the pre- and the post-typhoon surveys by a protective shore wall. However, strong winds and north-northeast surges brought by the typhoon caused erosion of the area and the volume, by 3,096 $m^2$ and 2,320 $m^3$. Accurate spatial databases of coastal zones based on integrated GPS GIS techniques and quantitative and qualitative analyses of topographical changes will help Korea develop systematic and effective countermeasures against coastal erosion.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.25 no.4
• /
• pp.418-425
• /
• 2014

This paper presents a Ku-Band Doppler Radar System to measure respiration and heart rate. It was measured by using simultaneous radar and ECG(Electrocardiogram). Arctangent demodulation without dc offset compensation can be applied to transmitted I/Q(In-phase & Quadrature-phase) signal in order to improve the RMSE(Root Mean Square Error) about 50 %. The power leaked to receiving antenna from the transmitting antenna is always generated because of continuously opening the transceiver of CW(Continuous Wave) Doppler radar. As the output power increase, leakage power has an effect on the SNR(Signal-to-Noise Ratio) of the system. Therefore, in this paper, leakage cancellation technique that adds the signal having the opposite phase of the leakage power to the leakage power was implemented in order to minimize the decline of receiver sensitivity. By applying the leakage cancellation techniques described above, it is possible to measure the heart rate and respiration of the human at a distance of up to 35 m. the heart rate of the measured data at a distance of 35 m accords with the heart rate extracted from the ECG data.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.18 no.4
• /
• pp.679-700
• /
• 1996

Inferior alveolar nerve dysfunction may be the result of trauma, disease, or iatrogenic injury. Inferior alveolar nerve injury is inherent risk in endodontic therapy, orthognathic surgery of the mandible, and extraction of mandibular teeth, particularly the third molars. The sensory disturbances of inferior alveolar nerve associated with such injury have been well documented clinical problem that is commonly evaluated by several clinical sensory test including Tinels sign, Von Frey test(static light touch detection), directional discrimination, two-point discrimination, pin pressure nociceptive discrimination, and thermal test. These methods used to detect and assess inferior alveolar nerve injury have been subjective in nature, relying on the cooperation of the patients. In addition, many of these techniques are sensitive to differences in the examiners experience and skill with the particular technique. Data obtained at different times or by different examiners are therefore difficult to compare. Prior experimental studies have used electro diagnostic methods(sensory evoked potential) to objectively evaluate inferior alveolar nerve after nerve injury. This study was designed with inferior alveolar nerve of rabbit. Several types of injury including mind, moderate, severe compression and perforation with 19 gauze, 21 gauze needle and 6mm, 10mm traction were applied for taking the sesory evoked ppterntial. Latency and amplitude of injury rabbit inferior alveolar nerve were investigated with sensory evoked potential using unpaired t-test. The results were as follows : 1. Intensity of threshold (T1) was $128{\pm}16{\mu}A$ : latency, $0.87{\pm}0.07$ microsecond : amplitude, $0.4{\pm}0.1{\mu}V$ : conduction velocity, 23.3 m/s in sensory evoked potential of uninjured rabbit inferior alveolar nerve. 2. Rabbit inferior alveolar nerve consists of type II and III sensory nerve fiber. 3. Latency was increased and amplitude was decreased in compression injury. The more injured, the more changed in latency and amplitude. 4. Findings in perforation injury was similar to compression injury. Waveform for sensory evoked potential improved by increasing postinjured time. 5. Increasing latency was prominent in traction injury rabbit inferior alveolar nerve. 6. In microscopic histopathological findings, significant degeneration and disorganization of the internal architecture were seen in nerve facicle of severe compression and 10mm traction group. From the above findings, electrophysiological assessment(sensory evoked potential) of rabbit injured inferior alveolar nerve is reliable technique in diagnosis and prognosis of nerve injury.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.18 no.1
• /
• pp.135-141
• /
• 2018

In this paper, we describe design and implementation of optimal smart home control system. Recent developments in technologies such as sensors and communication have enabled the Internet of Things to control a wide range of objects, such as light bulbs, socket-outlet, or clothing. Many businesses rely on the launch of collaborative services between them. However, traditional IoT systems often support a single protocol, although data is transmitted across multiple protocols for end-to-end devices. In addition, depending on the manufacturer of the Internet of things, there is a dedicated application and it has a high degree of complexity in registering and controlling different IoT devices for the internet of things. ARIoT system, special marking points and edge extraction techniques are used to detect objects, but there are relatively low deviations depending on the sampling data. The proposed system implements an IoT gateway of object based on OneM2M to compensate for existing problems. It supports diverse protocols of end to end devices and supported them with a single application. In addition, devices were learned by using deep learning in the artificial intelligence field and improved object recognition of existing systems by inference and detection, reducing the deviation of recognition rates.

• Proceedings of the Korean Society for Bioinformatics Conference
• /
• 2001.10a
• /
• pp.61-86
• /
• 2001

All cancers are caused by abnormalities in DNA sequence. Throughout life, the DNA in human cells is exposed to mutagens and suffers mistakes in replication, resulting in progressive, subtle changes in the DNA sequence in each cell. Since the development of conventional and molecular cytogenetic methods to the analysis of chromosomal aberrations in cancers, more than 1,800 recurring chromosomal breakpoints have been identified. These breakpoints and regions of nonrandom copy number changes typically point to the location of genes involved in cancer initiation and progression. With the introduction of molecular cytogenetic methodologies based on fluorescence in situ hybridization (FISH), namely, comparative genomic hybridization (CGH) and multicolor FISH (m-FISH) in carcinomas become susceptible to analysis. Conventional CGH has been widely applied for the detection of genomic imbalances in tumor cells, and used normal metaphase chromosomes as targets for the mapping of copy number changes. However, this limits the mapping of such imbalances to the resolution limit of metaphase chromosomes (usually 10 to 20 Mb). Efforts to increase this resolution have led to the "new"concept of genomic DNA chip (1 to 2 Mb), whereby the chromosomal target is replaced with cloned DNA immobilized on such as glass slides. The resulting resolution then depends on the size of the immobilized DNA fragments. We have completed the first draft of its Korean Genome Project. The project proceeded by end sequencing inserts from a library of 96,768 bacterial artificial chromosomes (BACs) containing genomic DNA fragments from Korean ethnicity. The sequenced BAC ends were then compared to the Human Genome Project′s publicly available sequence database and aligned according to known cancer gene sequences. These BAC clones were biotinylated by nick translation, hybridized to cytogenetic preparations of metaphase cells, and detected with fluorescein-conjugated avidin. Only locations of unique or low-copy Portions of the clone are identified, because high-copy interspersed repetitive sequences in the probe were suppressed by the addition of unlabelled Cotl DNA. Banding patterns were produced using DAPI. By this means, every BAC fragment has been matched to its appropriate chromosomal location. We have placed 86 (156 BAC clones) cytogenetically defined landmarks to help with the characterization of known cancer genes. Microarray techniques would be applied in CGH by replacement of metaphase chromosome to arrayed BAC confirming in oncogene and tumor suppressor gene: and an array BAC clones from the collection is used to perform a genome-wide scan for segmental aneuploidy by array-CGH. Therefore, the genomic DNA chip (arrayed BAC) will be undoubtedly provide accurate diagnosis of deletions, duplication, insertions and rearrangements of genomic material related to various human phenotypes, including neoplasias. And our tumor markers based on genetic abnormalities of cancer would be identified and contribute to the screening of the stage of cancers and/or hereditary diseases

• Composites Research
• /
• v.24 no.1
• /
• pp.24-30
• /
• 2011

Low-velocity impact can cause various damages which are mostly hidden inside the laminates or occur in the opposite side. Thus, these damages cannot be easily detected by visual inspection or conventional NDT systems. And if they occurred between the scheduled NDT periods, the possibilities of extensive damages or structural failure can be higher. Due to these reasons, the built-in NDT systems such as real-time impact monitoring system are required in the near future. In this paper, we studied the impact monitoring system consist of impact location detection and damage assessment techniques for composite flat and stiffened panel. In order to acquire the impact-induced acoustic signals, four multiplexed FBG sensors and high-speed FBG interrogator were used. And for development of the impact and damage occurrence detections, the neural networks and wavelet transforms were adopted. Finally, these algorithms were embodied using MATLAB and LabVIEW software for the user-friendly interface.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.33 no.4
• /
• pp.1479-1487
• /
• 2013

The river levee collapse and flood damages are dramatically increased due to the floods which caused by abnormal weather nowadays. The counterplan like TDR(Time Domain Reflectometry) river levee leaking exploration technique is needed to that levee failure causes of levee failure such as levee failure by penetration, piping, inadequate levee materials selection, poor compaction are almost 52% of the failure. This research practiced various comparing experiments of existing TDR(probe and tube types) and developing CAP type TDR to evaluate acrylic small CAP mould and low-cost TDR levee leaking monitoring system which was used probe type TDR. As the result, evaluated TDR system had 20cm critical exploration performance which was a leaking exploration performance, The functional ratio of TDR exploration sensitivity of dry density was sensitive more than 3 times than dry density, and weathered granite soil foundation water contents(w)-dielectric constant(${\epsilon}$) corelation formula was suggested to measure functional ratio on developing cap type TDR system.

• Investigative Magnetic Resonance Imaging
• /
• v.12 no.2
• /
• pp.89-99
• /
• 2008

Imaging assessment of prostate cancer is one of the most difficult sections of oncology imaging. Detecting, localizing and staging of the primary prostate cancer by preoperative imaging are still challenging for the radiologist. Magnetic resonance (MR) imaging provides excellent soft tissue contrast and is widely used for solid organ imaging, but results of preoperative imaging of the prostate gland with conventional MR imaging is unsatisfactory. Positron emission tomography and computed tomography (PET/CT) is the cornerstone in oncology imaging, but some limitations prohibit the assessment of primary prostate cancer with PET or PET/CT. Recent studies to overcome these insufficient accuracies of imaging evaluation of primary prostate cancers with advanced MR techniques and PET and PET/CT are reported. In this article, we review the imaging findings of prostate cancer on variable modalities, focused on MR imaging and PET/CT.

• Journal of Korea Spatial Information System Society
• /
• v.4 no.2 s.8
• /
• pp.55-64
• /
• 2002

VEfficient generation of road data is one of the most important issues in GIS (Geographic Information System). In this paper, we propose a hybrid approach for automatic generation of road data by combining mobile mapping and image processing techniques. Mobile mapping systems have a form of vehicle equipped with CCD camera, GPS, and INS. They can calculate absolute position of objects that appear in acquired image by photogrammetry, but it is labor-intensive and time-consuming. Automatic road detection methods have been studied also by image processing technology. However, the methods are likely to fail because of obstacles and exceptive conditions in the real world. To overcome the problems, we suggest a hybrid method for automatic road generation, by exploiting both GPS/INS data acquired by mobile mapping system and image processing algorithms. We design an estimator to estimate 3-D coordinates of road line and corresponding location in an image. The estimation process reduces complicated image processing operations that find road line. The missing coordinates of road line due to failure of estimation are obtained by cubic spline interpolation. The interpolation is done piecewise, separated by rapid change such as road intersection. We present experimental results of the suggested estimation and interpolation methods with image sequences acquired by mobile mapping system, and show that the methods are effective in generation of road data.