• Title/Summary/Keyword: Detection agent

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Nickel(II) Determination by Spectrophotometry Coupled with Preconcentration Technique in Water and Alloy Samples

  • Rekha, Dasari;Kumar, Jengiti. Dilip;Jayaraj, Bellum;Lingappa, Y.;Chiranjeevi, Pattium
    • Bulletin of the Korean Chemical Society
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    • v.28 no.3
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    • pp.373-378
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    • 2007
  • A micro organism Agrobacterium tumifacient as an immobilized cell on a solid support was presented as a new biosorbent in a simple and sensitive spectrophotometry determination of Ni(II) in various samples using 4-hydroxy benzaldehyde-4-bromophenyl hydrazone as a color developing agent (λmax 497 nm) at pH 4.0 ± 0.2. Beer's law was obeyed over the range of 0.01-0.1 μg L-1. The molar absorptivity and Sandell's sensitivity were 1.285 × 105 L mol-1cm-1 and 0.007245 μg cm-2 respectively. Under these conditions, the preconcentration factor obtained was 82, and the detection limit achieved was 0.05 μg L-1. The detailed study of various interfering ions made the method more sensitive and selective. The recovery of Ni(II) from various samples range from 97.75 to 99.35%. The present method was successfully applied for the determination of Ni(II) in spiked, natural water and alloy samples. The proposed method was compared with reported methods in terms of Student's ‘t'-test and Variance ratio ‘f'-test which indicates that there is no significant difference between proposed and literature method at 95% confidence level.

Involvement of Heat-stable and Proteinaceous Materials in the Culture of Pseudomonas putida JB-1 for the Inhibition of Tobacco mosaic virus Infection

  • Jeon, Yong-Ho;Kim, Jae-Hyun;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.24 no.3
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    • pp.328-336
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    • 2008
  • Out of various fungi and bacteria tested for inhibition of Tobacco mosaic virus(TMV) infection using Nicotiana tabacum cv. Xanthi-nc, a bacterial isolate JB-l, identified as Pseudomonas putida had a strong direct inhibitory activity against the TMV infection. Its systemic or indirect activity was also noted at more than a half level of the direct control efficacy. Disease severity was reduced significantly in the susceptible tobacco N. tabacum cv. NC 82 by the treatment of the bacterial culture filtrate, somewhat more by the pretreatment than by simultaneous treatment, probably by inhibiting the TMV transmission and translocation in the plants, showing negative serological, which responses in the viral detection by DAS-ELISA. TMV-inhibitory substances from P. putida JB-1 were water-soluble, stable to high temperature(even boiling), and to a wide range of pH. As proteinase K nullified their antiviral activity, the TMV inhibition activity of P. putida may be derived from proteinaceous materials. In electron microscopy, TMV particles treated with the JB-1 culture were shown to be shrunken with granule-like particles attached on them. All of these aspects suggest that P. putida JB-1 may be developed as a potential agent for the control of TMV.

Examination of seroprevalence and detection of Avian pneumovirus from layer hens in Gyeongbuk province (경북지역 산란계에서 Avian pneumovirus에 대한 항체가 및 바이러스 검출 조사)

  • Kim, Jung-Eun;Hwang, Ji-Young;Bae, Dong-Rok;Sung, Myoung-Suk;Kim, Soon-Tae;Kim, Sang-Yun
    • Korean Journal of Veterinary Service
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    • v.30 no.1
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    • pp.43-49
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    • 2007
  • Avian pneumovirus(APV), also known as avian rhinotracheitis(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis(TRT). The aim of this study was to establish the presence or absence of antibodies to APV by an enzyme-linked immunosorbent assay(ELISA) and confirm APV by a reverse transcriptase-polymerase chain reaction(RT-PCR). The tested serum and feces were collected from laying hens in Gyeongbuk province. The positive farms with antibody against APV by ELISA were 90(96.7%) of 93 and positive serum samples were 433(93.1%) of 465 different sera. By regional group, sera from Uiseong, Cheongsong and Bonghwa were noted as 100% positive and positive rates of samples from Yeongju, Andong and Yeongyang were 93.3%, 85.7% and 50%, respectively. However, APV was not detected in feces samples by RT-PCR.

Establishment of PCR to detect Bacillus anthracis in the experimentally infected soil and mice (PCR 기법을 이용한 인공감염토양 및 감염동물 장기로 부터 Bacillus anthracis의 검출)

  • Lee, Ji-youn;Yoo, Han-sang;Kim, Jong-yeom
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.574-580
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    • 1998
  • Anthrax caused by Bacillus anthracis is one of the most important zoonotic diseases in the worldwide. To control and prevent the disease effectively, several methods such as development of a fast and specific diagnostic method and vaccine, education etc, have been carried out. However, it still has a problem in the control and prevention. To control, the most important method is the prevention of direct or indirect contact of the causative agent with susceptible host. Therefore, we developed a fast and specific detection method, polymerase chain reaction, of B anthracis from soil and infected animals because the organism could survive long time in the environment including soil due to formation of spore. With the method, virulence genes of B anthracis were successfully amplified from experimentally infected soil and mice. Up to $4.2{\times}10$ of the organisms per gram could be detected with the PCR method from experimentally infected soil. These results suggested that this PCR method could be effectively used not only to detect B anthracis in soil and infected animal but also to provide the information to prevent the disease.

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A Ring-based Multicast Routing Architecture for Mobile Ad Hoc Networks (모바일 애드혹 네트워크를 위한 링 기반 멀티캐스트 라우팅 구조)

  • Heo Joon;Hong Choong Seon;Yang Yubai
    • The KIPS Transactions:PartC
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    • v.11C no.7 s.96
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    • pp.895-904
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    • 2004
  • Due to the frequent and unpredictable topology changes, multicast still remains as challenge and no one-size-fits-all protocol could serve all kinds of needs in mobile ad hoc network. In this paper, we propose a novel scheme of Hierarchical Eulerian Ring Oriented Multicast Architecture (HEROMA) over mobile ad hoc network. It has features that concentrate on efficiency and robustness simultaneously. It is also an application-driven proposal for hazard detection. Architectures including Eulerian ring, hierarchy and multicast agent are investigated in detail Simulation results different level of improvements on control traffic, end-to-end delay and packet delivery ratio by comparing with tree-based and mesh-based multicast protocols.

Synthesis of Silica-Core Gold-Satellite Nanoparticles and Their Surface-enhanced Raman Scattering Based Sensing Application (실리카 코어 금 위성입자의 합성 및 표면 증강 라만 산란을 기반으로 한 센서로의 응용)

  • Choi, Hyun Ji;Kim, Young-Kuk;Yoon, Seok-Young;Baek, Youn-Kyoung
    • Journal of Powder Materials
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    • v.21 no.6
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    • pp.441-446
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    • 2014
  • In this study, we synthesize silica-core gold-satellite nanoparticles (SGNPs) for the surface-enhanced Raman scattering (SERS) based sensing applications. They consist of gold satellite nanoparticles (AuNPs) fixed on the silica core nanoparticles, which sizes of AuNPs can be tunned by varying the amount of reactants (growth solution and reducing agent). Their surface plasmon resonance (SPR) properties were characterized by using UV-vis spectroscopy, showing that the growth of AuNPs on silica cores leads to the light absorption in the longer wavelength region. Furthermore, the size increase of AuNPs exhibited the dramatic change in SERS activity due to the formation of hot spots. The optimized SGNPs showing enhancement factor ${\sim}3.8{\times}10^6$ exhibited a detection limit of rhodamine 6G (R6G) as low as $10^{-8}M$. These findings suggest the importance of size control of SGNPs and their SPR properties to develop highly efficient SERS sensors.

Development of Loaded Stream Fire Extinguishing Systems for Underground Transmission Cables (지중송전선로 접속부용 미분무 강화액 소화설비의 개발연구)

  • Lee, Sung-Mo
    • Fire Science and Engineering
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    • v.22 no.1
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    • pp.93-98
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    • 2008
  • Full-scale fire extinguishment tests were conducted to develop loaded stream fire extinguishing systems for protecting underground transmission cables. The dimension of test mock-up was 5.5m height${\times}3m$ width ${\times}6m$ length, and six 154kV OF cables were piled up. Gasoline was used to ignite cates. Linear heat detection cables were installed on top of 154 kV OF cable and discharge nozzles were installed on the top and sidewalls, respectively. As a result, both surface fires and deap-seated fires were extinguished successfully within the specified 3 minutes by discharging loaded stream agent.

A Design of Invite Flooding Attack Detection and Defense Using SIP in VoIP Service (SIP을 이용한 VoIP 서비스에서의 Invite Flooding 공격 탐지 및 방어 기법 설계)

  • Yun, Snag-Jun;Kim, Kee-Chen
    • Proceedings of the Korean Information Science Society Conference
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    • 2011.06d
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    • pp.215-218
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    • 2011
  • VoIP(Voice over Internet Protocol) 서비스는 기존의 음성전화 서비스(Public Switched Telephone Network, PSTN)와 달리 IP 프로토콜을 이용한 저렴한 통신비용 등의 장점이 있는 음성통신 기술로써, 기존의 아날로그 음성전화 서비스를 대신하는 서비스이며, 새로운 인터넷 융합서비스로 많은 사용자가 이용하고 있다. 하지만 VoIP 서비스가 인터넷망을 이용함으로 IP Spoofing, DoS (Denial of Server) / DDoS(Distributed Denial of Service), 등의 여러 가지 보안의 문제점을 가지고 있다. VoIP 서비스에서 DDoS 공격은 Proxy 서버 등에 대량의 공격 메시지를 보냄으로써 서버의 자원을 고갈시켜 정상적인 서비스를 하지 못하게 한다. DoS, DDoS 공격 중 Invite Flooding 공격은 1분에 수천 개의 Invite 메시지를 보내 회선의 자원을 고갈시키는 공격이다. 특히 IP/Port 위조하여 공격 경우 공격 패킷 탐지하기 어려우므로 차단할 수 없다. 따라서 본 논문에서는 VoIP의 DoS/DDoS 중 하나인 Invite Flooding 공격 시 SIP Proxy Server에서 메시지 분산시키는 방법과 MAC Address와 사용자 번호 등 IP 이외의 고정적인 사용자 정보를 확인하여 공격을 탐지하고, 공격 Agent에 감염된 Phone을 공격차단서비스로 보내 복구시키는 방법을 제안한다.

Sensitive Fluorogenic Derivatization Method for Bile Acids by Dansyl Cadaverine (Dansyl Cadaverine을 이용한 담즙산류의 고감도 형광측정법)

  • Lee, Yong-Moon;Moon, Dong-Cheul;Han, Kun
    • Analytical Science and Technology
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    • v.11 no.6
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    • pp.429-435
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    • 1998
  • Free and glycine conjugated bile acids were detected fluoromatrically in high-performance liquid chromatography after derivatization with dansyl cadaverine. The coupling agent, diethyl phosphorocyanidate was used to form the amide bond between dansyl cadaverine and analytes. The dansyl derivatives of 8 bile acids were separated successfully on Cosmosil ODS column by using linear gradient elution of 20% MeOH-water/$CH_3CN$. The detection limits of cholic acid was reached 10 pg(S/N=5) per $1{\mu}l$ of injected volume. This new derivatization method would be applicable to detect the changes of bile acids in biological samples.

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Drug-induced Gingival Overgrowth Related to Sirolimus and Felodipine

  • Park, Youn-Jung;Lee, Joo-Hee;Kim, Young-Gun;Kwon, Jeong-Seung;Ahn, Hyung-Joon;Choi, Jong-Hoon
    • Journal of Oral Medicine and Pain
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    • v.42 no.1
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    • pp.20-24
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    • 2017
  • Drug-induced gingival overgrowth (DIGO) is an adverse drug reaction mainly described with three types of commonly prescribed drugs, namely, calcium channel blockers (CCBs) (nifedipine, diltiazem, and verapamil), anti-convulsants (phenytoin), and immunosuppressive agents (cyclosporine). Numerous reports have associated gingival overgrowth with the newer generation of immunosuppressive agents (tacrolimus, sirolimus, and everolimus), and CCBs (amlodipine, felodipine, nicardipine, and manidipine). Especially, patients concomitantly medicated with an immunosuppressive agent and CCB have a higher DIGO chance. Dentists need to be aware of drugs that induce gingival overgrowth, the possibility of DIGO, and risk factors, and also prevent the progression of DIGO by early detection of DIGO, consultation about the drug change, and the maintenance of strict dental hygiene regimes.