• Title/Summary/Keyword: Design development

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Development and Validation of Multiplex Polymerase Chain Reaction to Determine Squid Species Based on 16s rRNA Gene (오징어류 종 판별을 위한 다중 유전자 검사법 개발 및 검증)

  • Kim, Hyunsu;Seo, Yong Bae;Choi, Seong-Seok;Kim, Jin-Hee;Shin, Jiyoung;Yang, Ji-Young;Kim, Gun-Do
    • Journal of Food Hygiene and Safety
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    • v.30 no.1
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    • pp.43-50
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    • 2015
  • In this study, single PCR and multiplex PCR tests were examined for identification of four types of squid species (giant squid, cuttlefish, octopus, beka squid) purchased from fish market as well as aquatic processed products in Busan. To design the specific primers against each species, the nucleotide sequences of the mitochondrial 16s rRNA gene of Architeuthis dux, Todarodes pacificus, Enteroctopus dofleini, Enteroctopus megalocyathus, Uroteuthis chinensis, Uroteuthis duvauceli, Uroteuthis edulis groups were analyzed for the identification of each species registered in the GeneBank (www.ncbi.nlm.nih.gov) and have been used for comparative analysis. In order to obtain the size variation of amplified fragments on multiplex PCR, we designed KOJ-F, OJ-F, OCT-F, HAN-F, ALLR primers for each species. The optimal PCR conditions and primers were selected for four types of squid species to determine target base sequences in its PCR products. In the case of single PCR, giant squid was only amplified by KOJ-F/ALLR primer; cuttlefish was only amplified by OJ-F/ALLR primer; octopus was only amplified by OCT-F/ALLR primer; and beka squid was only amplified by HAN-F/ALLR primer. For multiplex PCR, the mixture of four kinds of genomic DNA (giant squid, cuttlefish, octopus, beka squid) been prepared as a template and used together with the mixture of KOJ-F/OJ-F/OCT-F/HAN-F/ALLR primers in the reaction. By the multiplex PCR, it is confirmed that four samples are correspond to multiple simultaneous amplicon. Finally, we validated the established methods of multiplex PCR in the aquatic processed products. Although the mitochondrial 16s rRNA primers used in this study was useful as a marker for detection of each species among them, the study indicated that the established multiplex PCR method can be more useful tool for monitoring the processed products.

A study on the comparison by the methods of estimating the relaxation load of SEM-pile (SEM파일의 이완하중 산정방법별 이완하중량 비교 연구)

  • Kim, Hyeong-Gyu;Park, Eun-Hyung;Cho, Kook-Hwan
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.20 no.3
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    • pp.543-560
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    • 2018
  • With the increased development in downtown underground space facilities that vertically cross under a railway at a shallow depth, the demand for non-open cut method is increasing. However, most construction sites still adopt the pipe roof method, where medium and large diameter steel pipes are pressed in to form a roof, enabling excavation of the inside space. Among the many factors that influence the loosening region and loads that occur while pressing in steel pipes, the size of the pipe has the largest impact, and this factor may correspond to the magnitude of load applied to the underground structure inside the steel pipe roof. The super equilibrium method (SEM) has been developed to minimize ground disturbance and loosening load, and uses small diameter pipes of approximately 114 mm instead of conventional medium and large diameter pipes. This small diameter steel pipe is called an SEM pile. After SEM piles are pressed in and the grouting reinforcement is constructed, a crossing structure is pressed in by using a hydraulic jack without ground subsidence or heaving. The SEM pile, which plays the role of timbering, is a fore-poling pile of approximately 5 m length that prevents ground collapse and supports surface load during excavation of toe part. The loosening region should be adequately calculated to estimate the spacing and construction length of the piles and stiffness of members. In this paper, we conducted a comparative analysis of calculations of loosening load that occurs during the press-in of SEM pile to obtain an optimal design of SEM. We analyzed the influence of factors in main theoretical and empirical formulas applied for calculating loosening regions, and carried out FEM analysis to see an appropriate loosening load to the SEM pile. In order to estimate the soil loosening caused by actual SEM-pile indentation and excavation, a steel pipe indentation reduction model test was conducted. Soil subsidence and soil loosening were investigated quantitatively according to soil/steel pipe (H/D).

Research on the Effect of the Control Methods of Irrigation Water on the Growth and Yield of Paddy Rice. (한발기에 있어서 용수관리 방법이 수도생육과 그 수량에 미치는 영향에 관한 연구)

  • 김시원
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.13 no.1
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    • pp.2177-2190
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    • 1971
  • This experiment was made to determienthe effect of various soil moisture contents in simulated drought conditions on different stages of rice growth. The drought conditions were developed at such three rice-growing stages as transplanting, immediately after transplanting and young ear forming. Three different lengths of drought periods, which are ten days, twenty days and thirty days, were applied for each growing stage of rice. The rice variety used this experiment is Nong-rim 29. This experiment was conducted at the university farm of the Kon-Kuk University during the period of $1968{\sim}1970$. Three reprications for each of 12 treatments and split plot design were employed in this study. Bottomless wood square boxes, $1^m{\times}1^m{\times}1^m$, were burried in the test plot and box top was covered with poloyethylene sheets to avoid natural rainfall drops. Standard plots were irrigated continuously with a water depth of 40mm/day and those of drought treatments were irrigated continuously up to the beginning of the planned drought period, and they were irrigated again with a depth of 40mm/day up to the maturing stage of rice. Other methods for rice raising followed those methods developed by the Field Crops Experiment Station of the Office of Rural Development. During this experiments, climatic conditions in regard to rainfalls, sunshine hours, and temperatures were observed. According to this observation, those values measured deviate slightly from the annual means. However the growing condition of rice plants were normal. The pH value of irritation water is nearly neutral, and soils in the test plots are relatively fertile, being similar to ordinary paddy soils. Analysis of variances for number of stalks, plan-height, ear sprouting date, length of stalks, ear length, number of ears per plant, fertility, grain weitght, weight of plant, and yield were carried out. The variances for plant height, ear sprouting date, length of stalk ear length, and yield has statistical significance under drought treatments applied at three different growing stages. The variance showing the effect of lengths of drought period is highly significant for all the treatments studied except that of grain weight. The interaction between drought periods and drought treatments at different growing stages is significant for plant height, stalk length, ear length, number of ears, fertility and yield, these results indicated that droughts at different growing stages have influence on plant height, ear length, yield, and length of drought period also has strong influence on all factors studied except grain weight. The combination of drought treatments at different rice growing stages and lengths of drought periods has different effects on various agronomic characteristics, including yield. Plant height under drought treatment practiced at transplanting stage is the lowest, and drought treatment applied immediately after transplanting resulted in the least number of stalks. The effect of different lengths of drought periods on plant height and number of stalks depends signis ficantly on increasing days of drought. Ear sprouting date tends to be delayed for one or two days undedrought treatments at transplanting period and with increasing days of drought. Better yield is secured in drought treatment applied immediately after transplanting. Adverse effect war observed when drought treatment was applied at ear forming period. These effects may be attributed to the alternation of irrigation and drought causing vigorous root activity. In general, yield linearly decreases as the length of the drought period increases. The results obtained in this study demonstrate that, in order to mimimize damage due to drought, and, to save irrigation water, paddy fields, immediately after transplanting, may be not irrigated, since there is sufficient moisture in the soil, and that sufficient irrigation water should be applied again in the ear forming stage of rice plant.

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A Study of the Curriculum Design Modelling Focused on the Combination of National Competency Standards and the Already-Accredited Course in the Department of Social Welfare in the Junior College (과정이수형 자격제도 운영 학과의 NCS 기반 교육과정 설계모형 연구 - 전문대학 사회복지과를 중심으로)

  • Park, Yong Woon;Kim, Kyoung Mee;Yoo, Tae Wan
    • The Journal of the Korea Contents Association
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    • v.16 no.2
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    • pp.652-665
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    • 2016
  • National Competency Standards or NCS is an educational system that emphasizes developing job-related abilities. Therefore it will be an effective solution in training field-oriented work forces if properly applied. However, in the department of social welfare, it is not easy to apply NCS to the curriculum since most academic subjects concerning social welfare focus not on practice but on theory and in addition, most of social welfare departments in junior colleges have an accredited curriculum for the 2nd degree of the social worker qualification. This means it is preposterous if NCS is applied to the curriculum without prior changes in the existing qualification system. So, this paper proposes a draft model to apply NCS to the already-accredited curriculum for the 2nd degree social workers in the junior colleges and details are as follows. Firstly, the competency units will be customized for the existing academic subjects in the curriculum rather than developing new subjects in accordance with NCS competency units. Secondly, some client-related competency units including children, seniors, the disabled are newly developed and then applied to the curriculum, which are crucial for the career development at the junior college level. Thirdly, the competency units are categorized into three types in accordance with the degree of job relevancy - type 1, type 2, type 3. Fourthly, four out of 11 basic job abilities are selected and then developed into academic subjects. Fifthly, all competency units concerning the main job market are regarded as one virtual competency unit and then arranged in the order of type 1s, type 2s and type 3s and then the scope of their study is adjusted to the job abilities required at the main job market.

The Effects of Corneal Eccentricity and Shape on Toric Soft Lens Rotation by Change of Postures (이심률 및 각막형상이 자세변화에 의한 토릭소프트렌즈의 회전에 미치는 영향)

  • Kim, So Ra;Hahn, Shin Woong;Song, Ji Soo;Park, Mijung
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.4
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    • pp.449-456
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    • 2013
  • Purpose: The present study aimed to investigate the effects of corneal eccentricity and shape on the rotational pattern of toric soft lens by the postural change of lens wearers. Methods: The corneal eccentricity of 41 eyes (aged 20s) having -1.0 D with-the-rule corneal astigmatism (WRCA) was measured, and then toric soft lenses were fitted with the amount of total astigmatism. In lying and straight postures, the rotation of toric soft lenses was recorded by a camera attached to slitlamp and analyzed. Results: Most toric soft lens designed with accelerated stabilization rotated to the temporal direction, which was the lying position direction, regardless of corneal eccentricity, and some lenses rotated to the nasal direction for high corneal eccentricity and corneal type of asymmetric bowtie. There was no correlation between the amount of rotation and corneal eccentricity right after of contact lens wearing in straight and lying posture, however, the amount of rotation was the greater for the cornea with the higher eccentricity after the subjects laying down for some period. The speed of lens rotation started to decrease after the subjects laying down, but the speed was not different according to corneal eccentricity difference. The amount of lens rotation for symmetric and asymmetric bowtie-typed corneas increased more than it for oval-typed cornea, and it was same even with time elapsing. The speed of lens rotation in lying posture was the slowest in asymmetric bowtie-typed cornea compared with other corneal types. Conclusions: From the present study, it was revealed that the rotational pattern of toric soft lens was affected by corneal eccentricity and corneal shape when the wearer's posture changed. Thus, it should be considered for the development of the fitting guideline and the design of toric soft lens.

Future Development Strategies for KODISA Journals: Overview of 2016 and Strategic Plans for the Future (KODISA 학술지 성장전략: 2016 개관 및 미래 성장개요)

  • Hwang, Hee-Joong;Lee, Jung-Wan;Youn, Myoung-Kil;Kim, Dong-Ho;Lee, Jong-Ho;Shin, Dong-Jin;Kim, Byung-Goo;Kim, Tae-Joong;Lee, Yong-Ki;Kim, Wan-Ki
    • Journal of Distribution Science
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    • v.15 no.5
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    • pp.75-83
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    • 2017
  • Purpose - With the rise of the fourth industrial revolution, it has converged with the existing industrial revolution to give shape to increased accessibility of knowledge and information. As a result, it has become easier for scholars to actively persue and compile research in various fields. This current study aims to focus and assess the current standing of KODISA: the Journal of Distribution Science (JDS), International Journal of Industrial Distribution & Business(IJIDB), the East Asian Journal of Business Management (EAJBM), the Journal of Asian Finance, Economics and Business (JAFEB) in a rapidly evolving era. Novel strategies for creating the future vision of KODISA 2020 will also be examined. Research design, data, and methodology - The current research will analyze published journals of KODISA in order to offer a vision for the KODISA 2020 future. In part 1, this paper will observe the current address of the KODISA journal and its overview of past achievements. Next, part 2 will discuss the activities that will be needed for journals of KODISA, JDS, IJIDB, EAJBM, JAFEB to branch out internationally and significant journals will be statistically analyzed in part 3. The last part 4 will offer strategies for the continued growth of KODISA and visions for KODISA 2020. Results - Among the KODISA publications, IJIDB was second, JDS was 23rd (in economic publications of 54 journals), and EAJBM was 22nd (out of 79 publications in management field journals). This shows the high quality of the KODISA publication journals. According to 2016 publication analysis, JDS, IJIDB, etc. each had 157 publications, 15 publications, 16 publications, and 28 publications. In the case of JDS, it showed an increase of 14% compared to last year. Additionally, JAFEB showed a significant increase of 68%. This shows that compared to other journals, it had a higher rate of paper submission. IJIDB and EAJBM did not show any significant increases. In JDS, it showed many studies related to the distribution, management of distribution, and consumer behavior. In order to increase the status of the KODISA journal to a SCI status, many more international conferences will open to increase its international recognition levels. Second, the systematic functions of the journal will be developed further to increase its stability. Third, future graduate schools will open to foster future potential leaders in this field and build a platform for innovators and leaders. Conclusions - In KODISA, JDS was first published in 1999, and has been registered in SCOPUS February 2017. Other sister publications within the KODISA are preparing for SCOPUS registration as well. KODISA journals will prepare to be an innovative journal for 2020 and the future beyond.

Development of Agent-based Platform for Coordinated Scheduling in Global Supply Chain (글로벌 공급사슬에서 경쟁협력 스케줄링을 위한 에이전트 기반 플랫폼 구축)

  • Lee, Jung-Seung;Choi, Seong-Woo
    • Journal of Intelligence and Information Systems
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    • v.17 no.4
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    • pp.213-226
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    • 2011
  • In global supply chain, the scheduling problems of large products such as ships, airplanes, space shuttles, assembled constructions, and/or automobiles are complicated by nature. New scheduling systems are often developed in order to reduce inherent computational complexity. As a result, a problem can be decomposed into small sub-problems, problems that contain independently small scheduling systems integrating into the initial problem. As one of the authors experienced, DAS (Daewoo Shipbuilding Scheduling System) has adopted a two-layered hierarchical architecture. In the hierarchical architecture, individual scheduling systems composed of a high-level dock scheduler, DAS-ERECT and low-level assembly plant schedulers, DAS-PBS, DAS-3DS, DAS-NPS, and DAS-A7 try to search the best schedules under their own constraints. Moreover, the steep growth of communication technology and logistics enables it to introduce distributed multi-nation production plants by which different parts are produced by designated plants. Therefore vertical and lateral coordination among decomposed scheduling systems is necessary. No standard coordination mechanism of multiple scheduling systems exists, even though there are various scheduling systems existing in the area of scheduling research. Previous research regarding the coordination mechanism has mainly focused on external conversation without capacity model. Prior research has heavily focuses on agent-based coordination in the area of agent research. Yet, no scheduling domain has been developed. Previous research regarding the agent-based scheduling has paid its ample attention to internal coordination of scheduling process, a process that has not been efficient. In this study, we suggest a general framework for agent-based coordination of multiple scheduling systems in global supply chain. The purpose of this study was to design a standard coordination mechanism. To do so, we first define an individual scheduling agent responsible for their own plants and a meta-level coordination agent involved with each individual scheduling agent. We then suggest variables and values describing the individual scheduling agent and meta-level coordination agent. These variables and values are represented by Backus-Naur Form. Second, we suggest scheduling agent communication protocols for each scheduling agent topology classified into the system architectures, existence or nonexistence of coordinator, and directions of coordination. If there was a coordinating agent, an individual scheduling agent could communicate with another individual agent indirectly through the coordinator. On the other hand, if there was not any coordinating agent existing, an individual scheduling agent should communicate with another individual agent directly. To apply agent communication language specifically to the scheduling coordination domain, we had to additionally define an inner language, a language that suitably expresses scheduling coordination. A scheduling agent communication language is devised for the communication among agents independent of domain. We adopt three message layers which are ACL layer, scheduling coordination layer, and industry-specific layer. The ACL layer is a domain independent outer language layer. The scheduling coordination layer has terms necessary for scheduling coordination. The industry-specific layer expresses the industry specification. Third, in order to improve the efficiency of communication among scheduling agents and avoid possible infinite loops, we suggest a look-ahead load balancing model which supports to monitor participating agents and to analyze the status of the agents. To build the look-ahead load balancing model, the status of participating agents should be monitored. Most of all, the amount of sharing information should be considered. If complete information is collected, updating and maintenance cost of sharing information will be increasing although the frequency of communication will be decreasing. Therefore the level of detail and updating period of sharing information should be decided contingently. By means of this standard coordination mechanism, we can easily model coordination processes of multiple scheduling systems into supply chain. Finally, we apply this mechanism to shipbuilding domain and develop a prototype system which consists of a dock-scheduling agent, four assembly- plant-scheduling agents, and a meta-level coordination agent. A series of experiments using the real world data are used to empirically examine this mechanism. The results of this study show that the effect of agent-based platform on coordinated scheduling is evident in terms of the number of tardy jobs, tardiness, and makespan.

Strain Improvement and Bioprocess Optimization for Enhanced Production of Haluronic Acid(HA) in Bioreactor Cultures of Streptococcus zooepidemicus (히알루론산 생산성 향상을 위한 Streptococcus zooepidemicus 균주 개량 및 발효조 배양공정 최적화)

  • Kim, Soo Yeon;Chun, Gie-Taek
    • Microbiology and Biotechnology Letters
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    • v.48 no.3
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    • pp.344-357
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    • 2020
  • Strain improvement and bioprocess development were undertaken to enhance hyaluronic acid(HA) production by Streptococcus zooepidemicus cells. Using a high-yielding mutant strain, statistical medium optimization was carried out in shake flask cultures, resulting in 52% increase in HA production (5.38 g/l) at the optimal medium composition relative to the parallel control cultures. For sufficient supply of dissolved oxygen (DO), which turned out to be crucial for enhanced production of HA, agitation system and speed were intensively investigated in 5 L bioreactor cultures. Increase in oxygen mass transfer coefficient (kLa) through increment of agitation speed (rpm) and 35% expansion of diameter of the newly-designed impellers showed significantly positive effects on HA production. By installing an expanded Rushton-turbine impeller for efficient break-down of sparged air, and an extended marine impeller above the Rushton-turbine impeller for efficient mixing of the air-born viscous fermentation broth, maximum amount of HA (9.79 g/l) was obtained at 450 rpm, 1.8 times higher level than that of the corresponding flask culture. Subsequently, the possibility of bioprocess scale-up to a 50 L bioreactor was investigated. Despite almost identical maximum HA production (9.11 vs 9.25 g/l), the average HA volumetric productivity (rp) of the 50 L culture turned out only 74% compared to the corresponding 5 L culture during the exponential phase, possibly caused by shear damages imposed on the producing cells at the high stirring in the 50 L culture. The scale-up process could be successfully achieved if a scale-up criterion of constant oxygen mass transfer coefficient (kLa) is applied to the 50 L pilot-scale bioreactor system.

Analysis of the Dose Distribution of Moving Organ using a Moving Phantom System (구동팬텀 시스템에 의한 움직이는 장기의 선량분포 분석)

  • Kim, Yon-Lae;Park, Byung-Moon;Bae, Yong-Ki;Kang, Min-Young;Lee, Gui-Won;Bang, Dong-Wan
    • The Journal of Korean Society for Radiation Therapy
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    • v.18 no.2
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    • pp.81-87
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    • 2006
  • Purpose: Few researches have been peformed on the dose distribution of the moving organ for radiotherapy so far. In order to simulate the organ motion caused by respiratory function, multipurpose phantom and moving device was used and dosimetric measurements for dose distribution of the moving organs were conducted in this study. The purpose of our study was to evaluate how dose distributions are changed due to respiratory motion. Materials and Methods: A multipurpose phantom and a moving device were developed for the measurement of the dose distribution of the moving organ due to respiratory function. Acryl chosen design of the phantom was considered the most obvious choice for phantom material. For construction of the phantom, we used acryl and cork with density of $1.14g/cm^3,\;0.32g/cm^3$ respectively. Acryl and cork slab in the phantom were used to simulate the normal organ and lung respectively. The moving phantom system was composed of moving device, moving control system, and acryl and cork phantom. Gafchromic film and EDR2 film were used to measure dose ditrbutions. The moving device system may be driven by two directional step motors and able to perform 2 dimensional movements (x, z axis), but only 1 dimensional movement(z axis) was used for this study. Results: Larger penumbra was shown in the cork phantom than in the acryl phantom. The dose profile and isodose curve of Gafchromic EBT film were not uniform since the film has small optical density responding to the dose. As the organ motion was increased, the blurrings in penumbra, flatness, and symmetry were increased. Most of measurements of dose distrbutions, Gafchromic EBT film has poor flatness and symmetry than EDR2 film, but both penumbra distributions were more or less comparable. Conclusion: The Gafchromic EBT film is more useful as it does not need development and more radiation dose could be exposed than EDR2 film without losing film characteristics. But as response of the optical density of Gafchromic EBT film to dose is low, beam profiles have more fluctuation at Gafchromic EBT. If the multipurpose phantom and moving device are used for treatment Q.A, and its corrections are made, treatment quality should be improved for the moving organs.

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A Study on Needs of 'Strolling (Yu)' at the Fine Arts Education - Focused on Oriental Painting - (미술교육에 있어서 '노닐음(遊)'에 대한 필요성 연구 - 동양화를 중심으로 -)

  • Jeong, Gyeong-Cheol
    • Journal of Science of Art and Design
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    • v.12
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    • pp.97-124
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    • 2007
  • The paper examined whether the fine arts education needed concept of 'Yu' that was suggested by, 'Soyoyu' of Jangja, a representative philosopher of Doka. At first, the paper defined concept of 'Yu' of Jangja as well as background and needs of the fine arts education, and investigated values of 'Yu' of the Oriental painting in aesthetic way to examine whether today's Yu concept can be applied to modern Oriental fine arts education to express. Chapter 2 examined concept and thought of 'strolling (Yu)', and Chapter 3 did background and needs of the fine arts education. Chapter 4 examined an access to practical technique education of the Oriental painting through 'strolling (Yu)': At first, the chapter investigated 'Heosil' of space concept that was researched at Chapter 2, 'Pilmuk' of expression technique, and 'Saeui' of state of spiritual canvas of painters. The findings were as follow: Firstly, when relation between Yu and Oriental paintings was investigated based on formative idea, 'Heo' reminded appreciators of association of ideas, hint and imagination, etc by, 'Sil' that other objects disclosed intrinsic attributes so that it indicated border of positive forgetfulness expressed by artists to have same border between 'Heo' and 'Yu'. Therefore, both 'Heo' and 'Sil' could build up expression as well as appreciation ability by experiencing formative idea to develop creativity and to build emotion and to cognize needs of the fine arts education. Secondly, the artistic state of 'Shin', 'Ki (Simjae)', 'Jeonshin' and, Saeui', etc could be expressed with strength and weakness of both Yin and Yang of Pilmuk. Therefore, the Pilmuk were linked even with creation of both Hyeongsa and artistic form of Saeui. Therefore, freedom at border of spiritual 'strolling' could produce creative power being expressed by thinking, natural appreciation ability, and education that could judge values of aesthetic culture. Therefore, cultivation of aesthetic eye, development of creativity, build up of formative ability and education of human nature, etc could keep identity of the Oriental fine arts education at various modern fine arts.

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