• Korean Journal of Microbiology
• /
• v.42 no.3
• /
• pp.195-198
• /
• 2006

Lethal toxin is a critical virulence factor of anthrax. It is composed two protein: protective antigen (PA) and lethal factor (LF). PA binds to specific cell surface receptors and, forms a membrane channel that mediates entry of LF into the cell. LF is a zinc-dependent metalloprotease, which cleaves MKKs [MAPK (mitogen-activated protein kinase) kinases] at peptide bonds very close to their N-termini. In this study, we suggest application of cell-based assays in the early phase of drug discovery, with a particular focus on the use of yeast cells. We constructed MEK1 expression system in yeast to determine LF activity and approached cell-based assay system to screen inhibitors, in which the results covering the construction of LF-substrate in yeast expression vector, expression, and LF-mediated proteolysis of substrate were described. These results could provided the basic steps in design of cell-based assay system with the high efficiency, rapidly and easy way to screening of inhibitors.

• Korea Journal of Hospital Management
• /
• v.7 no.4
• /
• pp.172-192
• /
• 2002

The purpose of this study is to ascertain whether the effect of introduction of OCS(Order Communication System) to the hospital is satisfied or not comparing the anticipated effect with the actual effect. For this purpose, a domestic hospital which has introduced and has been operating OCS for several years was chosen. Based on the internal data of S Hospital prepared before introducing OCS, researcher has analyzed the basic direction, design standard and status of operation after the introduction of OCS, etc. After analyzing the status of operations of several departments using OCS and interviewing with the chiefs of pertinent departments, a survey form was designed. Actual survey and interviews were conducted by the researcher for weeks to know whether doctors, nurses, medical technicians and clerks of the patient management dept. were satisfied with OCS and to find if they have any recommendations to improve OCS. Based on the analysis of survey, the effect of OCS was evaluated whether it has satisfied the anticipated effectiveness. For the question if they feel convenient in using OCS, doctors, nursing staffs in charge of ward and the staffs of billing dept. has answered that they were all satisfied(100%). The answers for the same question were relatively high in the case of nurses in charge of outpatient and staffs of radiography. Of course, there have been some nurses and staffs who complained for the inconvenience. However, overall satisfaction was high on the average. Some common problems occurred after the introduction of OCS were frequent errors due to instability of OCS system, paralysis of function of hardware on data back-up system and redundant investment due to erroneous choice of DB program in setting DB. It was also pointed out that lack of computer education and low participation of medical staffs has resulted in failure of developing effective software. As a result, it has lowered the efficiency of OCS. For example, some works have to be done by hands even after OCS. Based on the result of this research, recommendations to maximize the effect of OCS were presented as follows. First, strong leadership of CEO and active cooperation of doctors are mandatory. Second, all the process of hospital work should be analyzed and be redesigned in more efficient ways. Third, OCS should be designed to be user-based system which can be used efficiently by all staffs of the hospital. Forth, prior to the operation of OCS, proper tests of the program and trainings of the pertinent staff are required. Fifth, prior to the selection of hardware, BMT(Bench Marking Test) should be conducted. Sixth, before introducing OCS, staffs in charge of OCS should visit many hospitals operating the OCS system and take their cases into account.

• Korean Journal of Construction Engineering and Management
• /
• v.10 no.5
• /
• pp.123-134
• /
• 2009

Since 1970s, urethane waterproofing method is broadly used in rooftops, underground spaces, and sports stadium for its outstanding ultraviolet blockage, watertightness, and elasticity. However, development of slippage-resistance and endurance, improvement of function considering convenience and visually pleasing of users, urethane waterproofing method is necessary, since rooftops and underground spaces have slippage and external force risks. Therefore, many improved waterproofing methods are being developed and, recently, embo-thane waterproofing method, which applies embo-spray coating system, has been developed. This paper explains exposure, nonexposure, and floor-material of embo-thane waterproofing method, and then perform experimental study for comparison with urethane waterproofing method about tensile strength, coefficient of expansion, performance of bond, anti-abrasion, and slippage-resistance. In addition, the performance index was presented for the superiority of embo-thane waterproofing method compared to urethane by setting up evaluation criteria considering not only physical performance but also design side of embo-thane waterproofing method, and Value Analysis applying AHP. Also for an assessment considering uncertain result, Monte Carlo Simulation Method was used to operate reliability analysis through statistic approach method.

• Korean Chemical Engineering Research
• /
• v.45 no.3
• /
• pp.242-248
• /
• 2007

For an optimal design of automotive electric system, it is important to have a reliable modeling tool to predict the charge-discharge behaviors of the automotive battery. In this work, a two-dimensional modeling was carried out to predict the charge-discharge behaviors of a 12-V automotive lead-acid battery. The model accounted for electrochemical kinetics and ionic mass transfer in a battery cell. In order to validate the modeling, modeling results were compared with the experimental data of the charge-discharge behaviors of a lead-acid battery. The discharge behaviors were measured with three different discharge rates of C/5, C/10, and C/20 at operating temperature of $25^{\circ}C$. The batteries were charged with constant current of 30A until the charging voltage reached to a predetermined value of 14.24 V and then the charging voltage was kept constant. The discharge and charge curves from the measurements and modeling were in good agreement. Based on the modeling, the distributions of the electrical potentials of the solid and solution phases, the porosity of the electrodes, and the current density within the electrodes as well as the acid concentration can be predicted as a function of charge and discharge time.

• Journal of the Korean GEO-environmental Society
• /
• v.19 no.11
• /
• pp.31-37
• /
• 2018

Most of tall building systems are composed of above-ground structure and underground structure used for parking and stores. The underground structure may have a pronounced influence on tall building response, but its influence is still not well understood. In a widely referred report on seismic design of tall buildings, it is recommended to model the underground structure ignoring the surrounding ground and to impose input ground motion calculated considering the underground structure-soil kinematic interaction between at its base. In this study, dynamic analyses are performed on 1B and 5B basements. The motions at the base are calculated to free field responses. The motions are further compared to two procedures outlined in the report to account for the kinematic interaction. It is shown that one of the procedure fits well for the 1B model, whereas both procedures provide poor fit with 5B model analysis result.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.36 no.3
• /
• pp.176-182
• /
• 2020

Purpose: To evaluate the effects of load direction, number of implants, and alignment of implant position on stress distribution in implant, prosthesis, and bone tissue. Materials and Methods: Four 3D models were made to simulate posterior mandible bone block: two implants and 3-unit fixed dental prosthesis (FDP) with a pontic in the center (model M1), two implants and 3-unit FDP with a cantilever pontic at one end (model M2), FDP supported by three implants with straight line placement (model M3) and FDP supported by three implants with staggered implant configuration (model M4). The applied force was 120 N axially or 120 N obliquely. Results: Peak von Mises stresses caused by oblique occlusal force were 3.4 to 5.1 times higher in the implant and 3.5 to 8.3 times higher in the alveolar bone than those stresses caused by axial occlusal force. In model M2, the connector area of the distal cantilever in the prosthesis generated the highest von Mises stresses among all models. With the design of a large number of implants, low stresses were generated. When three implants were placed, there were no significant differences in the magnitude of stress between staggered arrangement and straight arrangement. Conclusion: The effect of staggering alignment on implant stress was negligible. However, the number of implants had a significant effect on stress magnitude.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.30 no.5
• /
• pp.895-907
• /
• 2020

With the recent increasing influence of multimedia content other than the text-based content, services that help to process information in content brings us great convenience. These services' representative features are searching and masking the sensitive data. It is not difficult to find the solutions that provide searching and masking function for text information and image. However, even though we recognize the necessity of the technology for searching and masking a part of the audio data, it is not easy to find the solution because of the difficulty of the technology. In this study, we propose web application that provides searching and masking functions for audio data using audio partitioning method. While we are achieving the research goal, we evaluated several speech to text conversion APIs to choose a proper API for our purpose and developed regular expressions for searching sensitive information. Lastly we evaluated the accuracy of the developed searching and masking feature. The contribution of this work is in design and implementation of searching and masking a sensitive information from the audio data by the various functionality proving experiments.

• Journal of the Korean Institute of Traditional Landscape Architecture
• /
• v.34 no.3
• /
• pp.1-17
• /
• 2016

The main aim of this study is to provide an overview of the characteristics the Oungak (五雲閣) area on the north of rear garden of Gyeongbokgung Palace which was constructed during the time of king Kojong. This study also consider the aspect of functional elements of Ocryucheon (玉流川) located in Changdeokgung Palace and how the Oungak area was used as a substitute for the Ocryucheon. The Oungak area is the private space of the king. It was built in a natural setting which used existing environment including spring water and the rock carved with inscriptions 'Cheonha-Jaeil-Bokji (天下第一福地). King Kojong tried to replace Ocryucheon with Oungak area which had played a important role of relaxation and leisure for royal family at the Ocryucheon to reconstructed Gyeongbokgung Palace. Despite being away from each other, Oungak area seems quite similar to Ocryucheon area. Oungak area and Ocryucheon have a common conditions of a location and structure of the building. Both constructed on the northernmost part of rear garden, composition of buildings and the design of waterway estimated to function as Curve-Stream Banquet. Oungak area was consisted of Ocryeonjeong (玉蓮亭) Pavilion, Oungak Building, Byeokhwasil (碧華室) Building and Cheonha-Jaeil-Bokji-Cheon (天下第一福地泉) Spring and Streams. Except Byeokhwasil Building, spatial compositions of Oungak area showed pattern that were similar to Ocryucheon area. The area was developed, moreover, to unconventional space that depended on the conditions of topography, slope, water system. First, The Ocryeonjeong Pavilion constructed to view the landscape of Seoul to Namsan Mountain. Second, the peculiar form of Cheonha-Jaeil-Bokji-Cheon Streams is estimated the shape imitated from Curve-Stream Banquet on Ocryuchon. Third, Oungak Building was constructed like a habitable house with Nongsanjeong Building in Ocryucheon. The Oungak area was constructed to improve Gyeongbokgung Palace, Consequently, the Oungak area specially characteristic of Joseon palaces's rear garden.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.12 no.2
• /
• pp.821-828
• /
• 2011

This paper developed the algorithm for improving the performance the auto pilot in the autonomous vehicle system consisting of the Track keeping control, the Automatic steering, and the Automatic mooring control. The automatic steering is the control device that could save the voyage distance and cost of fuel by reducing the unnecessary burden of driving due to the continuous artificial navigation, and avoiding the route deviation. During the step of the ship autonomic navigation control, since the wind power or the tidal force could make the ship deviate from the fixed course, the automatic steering calculates the difference between actual sailing line and the set course to keep the ship sailing in the vicinity of intended course. first, we could get the transfer function for the modeling of ship according to the Nomoto model. Considering the maneuverability, we propose it as linear model with only 4 degree of freedoms to present the heading angle response to the input of rudder angle. In this paper, the model of ship is derived from the simplified Nomoto model. Since the proposed model considers the maximum angle and rudder rate of the ship auto pilot and also designs the Fuzzy controller based on existing PID controller, the performance of the steering machine is well improved.

• The KIPS Transactions:PartD
• /
• v.11D no.6
• /
• pp.1247-1258
• /
• 2004

The LBS(Location-Based Service), which is based on individual's mobility, is required increasingly as mobile telecommunication and various infrastructures have developed rapidly. The technologies for LBS are location determination technology, service platform technology, contents provider technology and moving object database technology generally. Among these, service platform must be interoperable with location gate-way server and provide common function of billing, authentification, protect location information, privacy control, location trigger and intelligent acquisition and so on. The TTA(Telecommunications Technology Association) published specification that defines a standard protocol for safe and simple interface between LBS client and LBS platform and the OpenLS(Open Location Service) in OGC (Open GIS Consortium) released implementation specifications for providing Location based core services. In this paper, we implemented service platform for LBS which is able to interoperable with location gateway server and contents provider and is caracterized as follows. First, it could require and response location information from different types of location gateway server with same interface. Second, it complies with the standard interfaces with OpenLS 4 contents providers for core LBS. Third, it could provide location of wired phone as well as wireless mobile terminal compling with the standard protocol. Last, it could provide trajectorH information based past location as well as current location, because it is able to interoperable with moving object DBMS. This paper contributes to the construction and practical use of LBS by providing the method of implementation of service platform for LBS.