• Asian-Australasian Journal of Animal Sciences
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• v.16 no.3
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• pp.315-319
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• 2003

Random amplified polymorphic DNA (RAPD) analysis was done with 19 oligonucleotide primers to study genetic similarities and divergence among different types of Deoni breed of cattle viz., Balankya, Wannera and Waghya. Six random primers produced low to high numbers of polymorphic bands between pooled DNA of different Deoni types. Of the 48 RAPD markers obtained 33 were common to all Deoni types, 3 were individual specific and 12 were polymorphic for different Deoni types. The mean average percentage difference values among Deoni types showed that Balankya and Wannera had less genetic divergence when compared to Waghya.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.651-654
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• 2001

A study was made of the efficiency of ammonia N retention by Jowar kadbi (sorghum straw), initially 6.41% crude protein (CP), treated with 4% urea solution. After 30 days the CP in straw that was unchaffed and had been left uncovered was 10.02, and in chaffed straw that had been covered with a polythene sheet was 10.9%. The two treated straws were each fed to six crossbred (HF$\times$Deoni) calves, initially $12{\pm}2$ months old and $86.7{\pm}3.2kg$ bodyweight. They were also given two isocaloric (70% TDN) and isonitrogenous (20% CP) concentrate mixtures differing in calculated Rumen Degradable to Undegradable Dietary Protein ratio (RDP:UDP). Those fed the unchaffed uncovered treated straw (treatment C) received 65 RDP:35UDP and the other group (T1) received concentrate with a 55:45 ratio. The T1 group had the higher DM intake (p<0.01) in total (306 vs 268 kg), per day (4.1 vs 3.6 kg) and per unit bodyweight. Digestibility of DM, OM, CP and NDF, but not ADF, was higher in T1 and that group had the higher daily gain (517 vs 333 g) and higher total gain (38.8 vs 25.0 kg) over the 75 d of the feeding trial. It is concluded that chaffing and covering of Jowar kadbi treated with urea, not likely to be adopted by farmers because of financial constraints, does not confer important benefits. A concentrate supplement (estimated 45% of the CP as UDP) to calves given the treated straw has a beneficial effect on their growth and development.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.309-314
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• 2002

Random Amplified Polymorphic DNA (RAPD) assay was conducted to identify polymorphic markers in Amrithmahal, Krishna Valley, Hallikar, Deoni, Khillari, Ongole and Malnad Gidda breeds of South Indian cattle using twenty six primers. Of the 93 RAPD markers obtained, 53 were present in all breeds, 22 were individual specific and 18 were polymorphic for different breeds. Dual purpose breeds viz., Krishna Valley and Ongole showed less genetic divergence between them as compared to their genetic divergence from draft breeds viz., Amrithmahal, Hallikar and Khillari. Malnad Gidda was found to be a distinctly different from others studied.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.733-736
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• 2004

The study was carried out on eight Bos indicus cattle breeds namely, Sahiwal, Tharparkar, Nimari, Khilari, Deoni, Amritmahal, Hariana and Hilly cattle; two Bos taurus cattle breeds namely, Jersey and Holstein Friesian and Indicine${\times}$Taurine crossbred cattle to find out the polymorphic pattern of beta-lactoglobulin gene. The polymorphism at beta-lactoglobulin gene was detected by conducting PCR-RFLP studies on 398 bp fragment spanning over 104 bases of exon IV and 294 bases of intron IV. Two alleles A and B and three genotypes AA, AB and BB were observed in all the cattle breeds. The frequency of B allele was comparatively higher than that of A allele. The allelic frequency of A varied from 0.20 to 0.30 in Bos indicus cattle breeds and 0.19 to 0.34 in Bos taurus breeds while in crossbred cattle the frequency was estimated as 0.21. The weighted frequency of A allele was highest in Indian cattle and lowest in crossbred cattle while the frequency in taurine cattle was found to be in between indicus and crossbred cattle. The non-significant differences of allelic frequency amongst Bos indicus, Bos taurus and crossbred cattle was observed. The effect of genotype on fat percentage was also found to be non-significant in cattle.