• 한국수정란이식학회지
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• 제24권3호
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• pp.145-150
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• 2009

This study was conducted to examine the effects of human follicular fluid and gonadotropin (FSH+HCG+rhEGF) on in vitro maturation, fertilization and development of human immature oocytes. Cumulus-oocyte complexes (COCs) were collected following for in vitro fertilization and embryo transfer (IVF-ET) cycles of the patients. At the time of oocytes collection, oocytes were classified into MII, MI and GV in accordance with their appearance (MII: Fully mature oocyte at metaphase II of meiosis; MI: Nearly mature oocytes at metaphase I of meiosis; GV: Immature oocytes at prophase I of meiosis). After controlled ovarian stimulation using gonadotropin(FSH) and human chorionic gonadotropin (HCG) in 70 ICSI cycles, 158 MI to MII matured oocytes were intracytoplasmic sperm injection (ICSI) ${\sim}4$ h after in vitro culture and 553 MII oocytes were ICSI after denudation. The aspirated MI and GV oocytes were cultured in culture medium containing 10% (v/v) serum protein substitute (SPS), 10% (v/v) human follicular fluid (hFF) and 10% (v/v) serum protein substitute (SPS)+1 IU/ml FSH+10 IU/ml HCG+10 ng/ml recombinant human epidermal growth factor (rhEGF). The maturation rate of immature oocytes was similar among the three group. When maturation medium was supplemented with 10% SPS, 10% hFF or gonadotropins, the fertilization rate of in vitro matured oocytes was higher in 10% SPS (80.0%), but there was no statistical significance (78.2%; hFF, 76.9%; gonadotropin, p>0.05). The development rate of human embryos developed to $6{\sim}8$ cells were not significant difference in the medium containing SPS, hFF and gonadotropins (65.6%, 65.9% and 66.7%). The results of these study suggest that human follicular fluid and gonadotropins supplemented in the culture medium was not effected on the in vitro maturation, fertilization and development of human immature oocytes.

• Journal of Chest Surgery
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• 제8권2호
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• pp.159-168
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• 1975

We have experienced 61 cases of Clinically diagnosed tuberculous peripleural abscess which was surgically treated at St. Mary's Hospital of Catholic Medical College from Mar. 1963 to Feb. 1974. Out of them, 52 cases of pathologically confirmed tuberculous peripleural abscess were reviewed and its pathogenesis, treatment and so called "rib caries" were discussed. In the past, they have been described as a variety of the names, such as rib caries, cold abscess of the chest wall, pericostal abscess, lymphadenitis tuberculosa of the chest wall, chronic draining sinuses of the chest wall and other descriptive terms. Although it has been said that the tuberculous abscess on the chest wall developed as a secondary disease from so called "rib caries" but now it has been clear that this abscess occurred not from tuberculosis of the rib but from tuberculous lesion developed between end-othoracic fascia and parietal pleura usually following pulmonary tuberculosis and/or tuberculous pleurisy and the involvement of rib or ribs are secondary one from peripleural abscess, as we confirmed. Therefore we advocate that the nomination, rib caries, should not be used unless there is a primary tuberculous lesion on ribs. The results were as follows: 1. The highest age group of tuberculous peripleural abscess was ranged from the first to third decade (78%) 2. The location of tuberculous peripleural abscess on the chest wall were as follows, 31 cases on the anterior, 19 cases on lateral and 2 cases on the posterior. 3. On x-ray examination, abnormal findings including parenchymal tuberculous lesion and pleural changes were seen is 38 cases. 4. There was no destructive change of periosteum and rib in 23 cases of tuberculous peripleural abseess during operation. However the periosteal denudation and/or rib destruction were found in 29 cases. 5. The all cases of tuberculous peri pleural abscess developed from between endothoraclc fascia and parietal pleura, as we confirmed. With antituberculous therapy, operation should be radical by wide incision on the lesion including thorough curettage with proper drainage of Iiquified caseating materials and appropriate rib resection, if necessary.tion, if necessary.

• 한국농공학회지
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• 제17권3호
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• pp.3840-3847
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• 1975

With 30 excisting reservoirs in the Chin-Young area, the Sedimentation of the reservoirs has been calculated by comparing the present capacity with the original value, which revealed its reduced reservoir capacity. The reservoirs has a total drainage area of 3l4l ha, with a total capacity of 43.23 ha-m, and are short of water supply due to reduction of reservoir capacity, Annual sedimentation in the reservoir is relation to the drainage area, the mean of annual rainfall, and the slop of drainage area. The results of obtained from the investigation are summarized as follows: (1) A Sediment deposition rate is high, being about 7.03㎥/ha of drainage area, and resulting in the overage decrease of reservoir capacity by 16.1%. This high rate of deposition coule be mainly attributed to the serve denudation of forests due to disorderly cuttings of tree. (2) An average unit storageof 116mm as the time of initial construction is decreased to 95.6mm at present. This phenomena cause a greater storage of irrigation water, sinceit was assumed that original storage quantity itself was already in short. (3) A sediment deposition rate as a relation to the capacity of unit drainge area is as follow: Qs=1.27(C/A)0.6 and standard deviation is 185.5㎥/$\textrm{km}^2$/year. (4) A sediment deposition rate as a relation to the mean of annual rainfall is as follow: Qs=21.9p10.5 and the standard deviation is 364.8㎥/$\textrm{km}^2$/year. (5) A sediment deposition rate as a relation to the mean slop of drainage area is follow: Qs=39.6S0.75 and the standard deviation is 190.2㎥/$\textrm{km}^2$/year (6) Asediment deposition rate as a relation to the drainage area, mean of rainfall, mean of slope of drainage area is: Log Qs=0.197+0.108LogA-6.72LogP+2.20LogS and the standard deviation is 92.4㎥/$\textrm{km}^2$/year

• 동의생리병리학회지
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• 제28권2호
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• pp.169-177
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• 2014

This study aimed to investigate the relaxation effects and its underlying mechanisms of Epimedium koreanum Nakai(EK) in phenylephrine(PE) treated isolated rabbit corpus cavernosum smooth muscle. The dose-dependent relaxation responses of phenylephrine(PE, $1{\times}10^{-6}M$)-precontracted strips to EK at $0.01-3.0mg/m{\ell}$ were measured and also observed after endothelial denudation using organ bath. To analyze the underlying mechanisms of EK-induced relaxation, $N{\omega}$-nitro-L-arginine(L-NNA), methylene blue(MB), tetraethylammonium chloride(TEA), indomethacin(IM) were pretreated before EK extract infused into precontracted strips induced by PE. To investigate cytotoxic activity and nitric oxide(NO) concentration of EK extract on EA.hy926 cells, mitochondrial dehydrogenase activity(MTT) assay and nitric oxide detection kit were used. The cavernous strips were significantly relaxed by EK extract at $0.3mg/m{\ell}$, $1.0mg/m{\ell}$, $3.0mg/m{\ell}$ and the relaxation responses of PE-precontracted strips denuded endothelium also inhibited in comparison with intact endothelium. The pretreatment of L-NNA, MB, TEA reduced EK extract-induced endothelium-dependent relaxation, but the pretreatment of IM didn't affect EK extract-induced endothelium-dependent relaxation. When EK extract was applicated on EA.hy926 cells, the NO concentration was increased. Our findings have shown that EK extract exerts a relaxing effect on corpus cavernosum in part by suppressing influx of extracellular $Ca^{2+}$ through activating the NO-cGMP system.

• 암석학회지
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• 제2권2호
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• pp.104-121
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• 1993

Fission track (FT) thermochronological analyses on Mesozoic granites provide new information about cooling and uplift histories in Southeast Korea. Twenty-nine new FT sphene, zircon and apatite ages and seven track length measurements are presented for eleven granite samples. Measured mineral ages against assumed closure temperatures yield cooling rates for each sample. Relatively rapid (7-$15^{\circ}C$/Ma) and simple cooling patterns from the middle Cretaceouss (ca. 90-100 Ma) granites are caused mainly by a high thermal contrast between the intruding magma and country rocks at shallow crustal levels (ca. 1-2.5 km-depths). On the contrary, a slow overall cooling (1-$4^{\circ}C$/Ma) of the Triassic to Jurassic granites (ca. 250-200 Ma), emplaced at deep depths (>>9 km), may mainly depend upon very slow denudation of the overlying crust. The uplift history of the Triassic Yeongdeog Pluton in the Yeongyang Subbasin, west of the Yangsan Fault, is characterized by a relatively rapid uplift (~0.4 mm/a) before the total unroofing of the pluton in the earliest Cretaceous (~140 Ma) followed by a subsidence (~0.2mm/a) during the Hayang Group sedimentation. Stability of original FT zircon ages (156 Ma) and complete erasure of apatite ages suggest a range of 3 to 5.5 km for the basin subsidence. Since 120 Ma up to present, the Yeongyang Subbasin has been slowly uplifted (~0.04 mm/a). The FT age patterns of Jurassic granites both from the northeastern wing of the Ryeongnam Massif and from the northern edge of the Pohang-Kampo Block indicate that the two geologic units have been slowly uplifted with a same mean rate (~0.04 mm/a) since early Cretaceous. Estimates of Cenozoic total uplifts since 100 Ma are different: Ryeongnam Massif (~6 km)=Pohang-Kampo Block (~6 km)>Yeongyang Subbasin(~4 km).

• 암석학회지
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• 제2권2호
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• pp.122-129
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• 1993

We report Rb-Sr whole rock, K-Ar and fission track mineral ages for the Chuncheon granite in the Precambrian Gyeonggi massif. The Rb-Sr whole rock define an age of $196{\pm}9$ Ma with an initial ratio of $0.7159{\pm}0.0006$, suggesting that the granitic magma might have been generated from crustal sources (S-type), or probably mixed mantle and crustal materials, and emplaced into the massif in the late Triassic or the early Jurassic. K-Ar mineral ages of hornblende, muscovite and biotite are ~210 Ma, ~180 Ma and 166-170 Ma respectively, and fission track zircon and apatite ages are 65-70 Ma, ~35 Ma respectively. These ages indicate that the granitic magma might have been emplaced at about 7 to 9 km from the paleosurface, and rapidly cooled down up to $300^{\circ}C$ until middle Jurassic (~170 Ma) with a rate of about $10^{\circ}C$/Ma, due to thermal difference between the magma and the wall rock. During middle Jurassic to late Cretaceous (about 170-70 Ma), the granite pluton is assumed to have uplifted to 4 to 6 km level under the paleosurface with a rate of 30 m/Ma and slowly cooled down with a rate of about $1^{\circ}C$/Ma owing to relatively slow denudation of the massif. In late Cretaceous to the present, the pluton might have more rapidly uplifted to the present level with a rate of 85 m/Ma and rapidly cooled down with a rate of about $3^{\circ}C$/Ma compared to those of middle Jurassic to late Cretaceous time because of extensive igneous activities accompanied by tectonism in the Gyeonggi massif.

• 대한수의학회지
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• 제42권2호
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• pp.153-162
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• 2002

For the induction of arthropathy, 5% hydrogen peroxide($H_2O_2$) was injected for 5 weeks into the intraarticular space of the New Zealand white rabbits to damage articular cartilage. Alginic acid of low molecular weight (2%) made from macromolecular alginate treated with enzyme was administered into articular space at the dose of 5 mg/kg twice a week for 3 and 6 weeks using 1 ml syringe and 26 G needle. Saline was injected for the control. Tissues surrounding the articulation were obtained for the measurements of superoxide dismutase(SOD) activity as a major antioxidant enzyme and malondialdehyde (MDA) as a lipid peroxidation level. Histopathologic examination on the surface of articular cartilage was carried out. Data showed that injection of hydrogen peroxide for 5 weeks had led to the induction of free radical damage and of articular cartilage change as confirmed by microscopic observation. The application of hydrogen peroxide caused a gradual increase in the SODs and MDA. These patterns were similar after 3 and 6 weeks of alginate treatment. Furthermore, microscopic examinations revealed that hydrogen peroxide caused flaking, fibrillation, fissuring, denudation, and hypocellularity in the articular surfaces. In conclusion, lipid peroxidation was demonstrated in the articular cartilage by the administration of hydrogen peroxide in the rabbit model. This lipid peroxidation could be caused by oxygen free radicals. The histologic and enzymatic correlations on lipid peroxidation in the articulation have provided a better understanding of arthropathy. It is possible to take advantage of these findings to evaluate effective alginate dosage more efficiently.

• Journal of Chest Surgery
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• 제24권7호
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• pp.637-646
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• 1991

Effect of noradrenaline and endothelium on the high K+ or Ach-induced contraction were investigated in the pig myocardial coronary artery. The helical strip of isolated pig myocardial coronary artery was immersed in the Tris-buffered Tyrode`s solution equilibrated with 100% O2 at 37oC and its isometric tension was measured. High K+ and Ach-induced contraction were dose-dependent. By denuding the endothelium, dose-contraction curve of K+ was not shifted significantly to the left and upward, but that of Ach was shifted significantly to same direction 25 mM K+ - and Ach-induced contraction were relaxed by norepinephrine[NE]. NE-induced relaxation was blocked by the pretreatment of propranolol, which was known as b-adrenoceptor blocker. And, phenylephrine known as a-adrenoceptor agonist, and clonidine known as a-adrenoceptor agonist increased the 25mM K+ - induced contraction respectively. Denuding of endothelium did not show any significant effects on NE-induced relaxation and contraction increased by phenylephrine and clonidine. Tyramine increased 25mM K+ - induced contraction further. The contractile response by tyramine on the 25mM K+ - induced contraction was not blocked by the pretreatment of phentolamine, but was partially blocked by the pretreatment of atropine. From the above results, it is suggested that activation of a1-and a2-adrenoceptors induce the contraction, activation of b-adrenoceptors induce the relaxation, and NE-induced relaxation is mainly due to activation of b-adrenoceptors. Also it is suggested that denudation of endothelium did not influence NE-induced relaxation, but influence Ach-induced contraction in the pig myocardial coronary artery.

• 한국산림과학회지
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• 제78권3호
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• pp.302-313
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• 1989

관악산(冠岳山) 암석산지(岩石山地)에서 지표화(地表火)가 발생(發生)한지 5년(年)이 경과(經過)한 후(後) 토양조건(土壤條件) 및 삼림식생(森林植生) 변화(變化)에 미친 영향(影響)을 밝히고자 1988년(年) 7월(月)에 실시(實施)한 조사결과(調査結果)는 다음과 같다. 유기물함량(有機物含量), pH, 전질소함량(全窒素含量)은 산불발생(發生) 후(後) 3년(年)까지는 증가(增加)를 나타낸 후(後) 안정(安定)을 보였다. 5년간(年間) 초본(草本), 목본식물(木本植物)의 상대우점치(相對優占値) 변동(變動)에 의하여 출현종(出現種)들을 산불에 민감종(敏感種), 내성종(耐性種), 수입종(侵入種), 무반응종(無反應種)으로 구분(區分)할 수 있었다. 종다양도지수(種多樣度指數), 유사도지수(類似度指數) 등(等) 다양성지수(多樣性指數)의 변동(變動)을 고려(考慮)할 때, 산불 후(後) 5년(年)동안에 종구성측면(種構成側面)의 회복(回復)은 이루어 졌으나 수관울한(樹冠鬱閑), 임목성장(林木成長) 등(等) 식생발달측면(植生發達側面)에서의 회복(回復)은 아직 이루어지지 않은 것으로 판단(判斷)된다.

• 대한지리학회지
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• 제44권1호
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• pp.31-55
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• 2009

이 연구에서는 한반도 전체의 수치고도모델 분석을 통해 평탄지의 공간적 분포를 살펴보고, 평탄지의 유형을 분류한 뒤, 그 결과를 토대로 평탄지의 형성과정에 관한 일반모델을 제시하였다. 한반도에서는 산지와 뚜렷하게 대비되는 평탄지가 존재하고 있으며, 그 형태적 특성과 위치를 종합적으로 고려할 경우, 용암대지와 하해혼성평탄지, 구릉성평탄지, 산간분지, 고위평탄지, 하천주변평탄지 등으로 분류가 가능하다. 평탄지의 특성은 융기 및 해수면 변화와 관련된 기준면의 변동과 지질단위들의 위상관계에 의해 결정되며, 이들 평탄지들이 독립적으로 존재하는 것은 아니가 하천을 따른 삭박환경의 변화에 따라 동적으로 연계되어 있다. 이 연구에서 제시하는 평탄지 형성의 일반모델은 과거 개별적으로 이루어져 왔던 한반도의 평탄면과 각종 침식지형, 그리고 단구지형에 대한 연구들이 평탄지의 분포와 특성을 통해 통합될 수 있는 가능성을 제시해 준다.