• Journal of Periodontal and Implant Science
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• 제31권2호
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• pp.371-388
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• 2001

A novel glucanhydrolase(DXAMase) from a mutant of Lipomyces starkeyi(KSM 22) has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependentadherent microbial film and DXAMase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi DXAMase are desirable for its application as a dental plaque control agent. This study was performed to determine the adjunctive oral hygiene benefits and safety of dextranase(Lipomyces starkeyi KSM 22 DXAMase)-containing mouthwash when used alongside normal tooth-brushing. This 6-month clinical trial was placebo-controlled double-blind design evaluating 1U/ml dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 3 and 6 months, subjects were scored for plaque accumulation(Turesky modification of Quingley-Hein's plaque index), gingivitis status($L\ddot{o}e$ and Silness gingival index), and tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice daily after toothbrushing. All the groups showed significant increase in plaque accumulation since 1 month of experiment. During 6 months' period, the Dextranase mouthwash group showed the least increase in plaque accumulation, compared to the Chlorhexidine mouthwash and placebo groups. As for gingival inflammation, all the groups showed significant increase during 6 months of experiment. The Experimental group(Dextranase mouthwash) also showed the least increase in gingival index score, compared to the Positive control(Chlorhexidine mouthwash)as well as the Negative control(placebo)groups. Whereas the tooth stain was increased significantly in the Positive control group, compared to the baseline score and the Negative controlgroup since 3 months of mouthrinsing. It was significantly increased after 6 months in the Experimental group, still less severe than the Positive control group. As for the oral side effect, the Experimental group showed less tongue accumulation, bad taste, compared to the Positive control group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase provided adjunctive benefits to toothbrushing, comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, with long-term use of the mouthwash. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.

• 한국치위생학회지
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• 제13권2호
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• pp.271-277
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• 2013

Objectives : The purpose of this study was to evaluate the effects of incremental dental care(IDC) program on kindergarten children. Methods : The subjects were 46 kindergarten children aged 6 who wished participation in IDC program during 5 weeks at oral health center in G-college from March to May, 2012. The data of subjects were examined into oral conditions such as toothbrushing method and frequency, modified O'leary plaque index and dental caries activity before and after IDC program. The collected data were analyzed by SPSS statistical package version 19.0. Results : 1. The rolling and fones methods of toothbrushing increased from 10.8% to 91.3% after IDC program(p<.001). The change of toothbrushing frequency showed that over 3 times a day increased from 52.2% to 76.0% after IDC program(p<.01). 2. The modified O'leary plaque index of before toothbrushing education increased from 1st visit(score 32.19) to 5th visit(score 57.14) and after toothbrushing education increased from 1st visit(score 64.45) to 5th visit(score 78.27) during IDC program(p<.001). 3. As a results of dental caries activity test before and after IDC program, it was found that the bacterial numbers in S.mutans and Latobacillus reduced from 0.74, 0.70 to 0.28, 0.41 (p<.01) and the saliva buffering capacity increased from 1.50 to 2.02(p<.001). Conclusions : It is considered very necessary that IDC program should be maintained and extended to dental clinic so as to enhance the oral health state of kindergarten children.

• 보건교육건강증진학회지
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• 제26권2호
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• pp.25-33
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• 2009

Objectives: Smoking is related to periodontal disease and periodontal therapy. So the aim of this study was to investigate the effects of professional tooth cleaning and plaque control instruction (PT & PCI) for smoking behavior. Methods: A total of 151 adults were investigated using the O'Leary Plaque Index (PI), $L\ddot{o}e$ & Silness gingival index (GI) and the number of sextants possessing periodontal pocket (SPP). And adults were given a through dental scaling and Watanabe method for dental plaque control. Follow up examination were conducted after 3 months and compared the pre and post- status. The collected data were analyzed with t-test, paired t-test and one-way analysis of variance. Results: Regardless of smoking behavior, improving effects were identified after PT & PCI on PI, GI and SPP in the whole population. However, the effects of GI improvement were significant in the smoking group alone; those of PI improvement were most significant in the non-smoking group; and those of SPP improvement were more significant in non-smoking and pre-smoking groups than in the smoking group. The shorter period of smoking and the smaller amount of smoking, the greater effects of PT & PCI by smoking-related characteristics. Conclusion: Smoking cessation instruction should necessarily be included in oral health education in that smoking is an important factor to consider in prevention of periodontal diseases and periodontal therapies.

• 치위생과학회지
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• 제15권6호
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• pp.696-702
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• 2015

본 연구는 유치원에 다니는 아동 총 59명을 대상으로 Q-scan을 이용한 구강보건효과를 평가하고자 하였다. 유아용 면접설문지와 Quigley & Hein index를 측정하여 다음과 같은 결과를 얻었다. 아동의 치면세균막지수는 Q-scan을 이용한 중재군에서 12% 더 유의하게 감소한 것으로 나타났다(p<0.001). 구강보건지식은 두 집단 모두 불소에서 유의한 차이가 나타났으며(p<0.05), 대조군에서는 치아에 나쁜 음식에 대한 정답률도 유의하게 증가하였다(p<0.05). 구강보건태도에서 중재군은 모든 변수에서 유의한 차이가 나타났으며(p<0.05), 대조군에서는 정기 구강검진, 칫솔질 횟수에서 유의한 차이가 나타났다(p<0.05). 구강보건행동에서 중재군은 칫솔 잡는 법에서는 정답률이 증가하고, 알맞은 치약 짜기에서는 정답률이 감소하는 유의한 결과가 나타났으며(p<0.05), 대조군은 모든 변수에서 유의한 차이가 나타나지 않았다. 이러한 결과를 종합해 보면 Q-scan은 구강보건교육 시 아동들의 동기 부여를 위한 적절한 도구로 활용될 수 있을 것으로 생각된다.

• 한국학교보건학회지
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• 제27권1호
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• pp.1-6
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• 2014

Purpose: The purpose of this study was to investigate the effect of oral health education on adolescents. Methods: The subjects were middle school students in a health promoting school in Gunpo, Gyeonggi Province. Before giving the students oral health instruction, their modified Patient Hygiene Performance index (PHP index) was measured using disclosing solution. Then, the students were taught the right way to brush teeth (Rolling method) and use oral hygiene devices such as dental floss, tongue cleaner and interdental brush. Their modified PHP index was re-measured three days after and seven days after the education to evaluate the effect and persistency of oral health education. of the total 116 students, 48 students who completely filled out the questionnaire and participated in the whole process, photo shooting and three times of PHP index measurement, were included in the final analysis. Results: The students' modified PHP index significantly decreased three days after the education. However, the index slightly went back up seven days after the oral health education. Conclusion: Oral health education was effective in reducing dental plaque which remained after brushing. Continuous education about and constant motivation for oral hygiene turned out to be critical to maintain the impact of oral health education. Therefore, it is necessary to develop educational contents which can constantly motivate teenagers.

• Journal of Periodontal and Implant Science
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• 제34권1호
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• pp.195-204
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• 2004

A novel glucanhydrolase from Lipomyces starkeyi KSM 22 has been suggested as a promising anti-plaque agent because it has been shown to have additional amylase activity and mutanase activity besides dextranase activity and to strongly bind to hydroxyapatite. Mouthrinsing with Lipomyces starkeyi KSM 22 glucanhydrolase solution was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were less frequent and less intense in human experimental gingivitis. In this study, Lipomyces starkeyi KSM 22 glucanhydrolase mouthrinses (1 and 2 unit/ml) were compared with a control mouthrinse (commercial 0.01% benzethonium chloride mouthrinse, $Caregargle^{(R)}$, Hanmi Pharmaceuticals) in the ability to inhibit plaque formation. A 3-replicate clinical trial using 4-day plaque regrowth model was used. Fifteen volunteers were rendered plaque-free on the 1st day of each study period, ceased toothcleansing, and rinsed 2X daily with allocated mouthrinse thereafter. On day 5, plaque accumulation was scored and the washout periods was 9 days for the next trial. Lipomyces starkeyi KSM22 glucanhydrolase(1 unit and 2 unit)- containing mouthrinse resulted in Significantly lower plaque formation in plaque area and thickness, compared to the control mouthrinse. There was no significant difference in plaque inhibition between enzyme-mouthrinses at 2 different concentrations used. This glucanhydrolase- containing mouthwash resulted in significantly lower plaque area severity index score and tended to have lower plaque thickness severity index score than those of control mouthrinse. But there was no significant difference according to the enzyme concentration. From these results, Lipomyces starkeyi KSM 22 glucanhydrolase-containing benzethonium chloride mouthrinse has greater anti-plaque effect than the commercial mouthrinse alone. Therefore this glucanhydrolase preparation is a promising agent for new mouthwash formulation in the near future.

• Journal of Korean Biological Nursing Science
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• 제17권2호
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• pp.150-158
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• 2015

Purpose: This study aimed to evaluate the effects of gargling with S-solution and gargling with A-solution on salivary pH, coated tongue, and dental plaque index in preschool children. Methods: Non-equivalent control group pretest-posttest design was used to select the participants. 99 preschool children were divided into three groups. Dependent variables were recorded at baseline, 30 minutes, and 7 days after the first treatment was given. The data were analyzed using $X^2$-test, ANOVA, and repeated measures of ANOVA. Results: There were no significant differences in dependent variables in pre-test. However, the salivary pH in the S-solution group had significantly increased after 30 minutes (p<.05) and then again 7 days (p<.01) after the first treatment. Also, the S-solution and A-solution groups had greater decrease in dental plaque index after 30 minutes and again 7 days after the first treatment (p<.001) than the control group. With respect to coated tongue, there were no significant differences among the three groups. Conclusion: The results of this study indicate that essential oil gargling after brushing is helpful in improving oral health due to auxiliary oral hygiene effects with natural products. Specifically, gargling with S-solution is more effective than A-solution on oral health in preschool children by neutralizing salivary pH and reducing dental plaque index.

• The Journal of Advanced Prosthodontics
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• 제8권4호
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• pp.313-320
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• 2016

PURPOSE. The aim of this study was to evaluate the clinical findings and patient satisfaction on implant overdenture designed with Locator implant attachment or Locator bar attachment in mandibular edentulous patients. MATERIALS AND METHODS. Implant survival rate, marginal bone loss, probing depth, peri-implant inflammation, bleeding, plaque, calculus, complications, and satisfaction were evaluated on sixteen patients who were treated with mandibular overdenture and have used it for at least 1 year (Locator implant attachment: n=8, Locator bar attachment: n=8). RESULTS. Marginal bone loss, probing depth, plaque index of the Locator bar attachment group were significantly lower than the Locator implant attachment group (P<.05). There was no significant difference on bleeding, peri-implant inflammation, and patient satisfaction between the two denture types (P>.05). The replacement of the attachment components was the most common complication in both groups. Although there was no correlation between marginal bone loss and plaque index, a significant correlation was found between marginal bone loss and probing depth. CONCLUSION. The Locator bar attachment group indicates lesser marginal bone loss and need for maintenance, as compared with the Locator implant attachment group. This may be due to the splinting effect among implants rather than the types of Locator attachment.

• 대한치과교정학회지
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• 제40권2호
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• pp.95-105
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• 2010

교정치료 중 구강 내 환경 변화를 파악하기 위하여 교정치료 시작 후 24개월까지의 임상적 구강위생지수 및 타액, 구강 내 미생물의 변화양상을 평가하였다. 30명의 환자를 대상으로 하여, 임상적 구강위생지수(DMFS [decayed, missing and filled surfaces], O'Leary's plaque index, plaque pH), 타액의 변화(비자극/자극시의 타액량, 완충능(buffer capacity), pH, 잠혈(occult blood)류량, 구강 내 미생물(Streptococcus mutans, Lactobacillus) 변화를 파악하였다. 추가적으로 설문지를 이용하여 구강위생관리 및 식습관을 평가하였다. 분산분석, 회귀분석 및 Spearman의 상관분석을 통해 데이터를 분석하였다. 교정치료 도중 DMFS 값은 유의성있게 증가한 반면, plaque index는 감소하였고, plaque pH는 산성을 나타내었다 (p = 0.23). 비자극 시 타액량은 치료 중 유의성있게 변화하였으며 (p = 0.13), 자극 시의 타액량은 여성에서 장치 부착 이후 증가하는 양상을 보였다. Buffer capacity는 남성에서 치료 도중 감소되었으며, 타액의 pH는 유지되었다. 구강 내 미생물 및 잠혈류량은 고위험 수준까지 증가하였으며 성별의 차이는 나타나지 않았다 (p > 0.05). 치료 전 plaque와 구강위생용품의 사용 (r = 0.429; p = 0.018), 최종 DMFS와 비자극 시의 타액량 (r = -0.372; p = 0.043) 간에 뚜렷한 상관관계가 확인되었다. 교정장치의 부착으로 인해 구강 내 미생물은 증가하고, plaque pH는 산성화되어 치주조직의 손상이 일어나게 되었다. 또한 Buffer capacity는 변하지만, 치료 기간중의 타액의 pH는 유지되었다.

• 대한치과교정학회지
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• 제29권3호
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• pp.361-374
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• 1999

구강내 고정식 교정장치의 장착으로 인해 치은염 및 치주염, 법랑질 탈회 및 치아 우식증, 치근 흡수, 치수변화 등 일시적 혹은 영구적 손상이 야기될 수 있다. 이러한 부작용발생의 원인으로 치태의 증가, 세균수의 증가와 조성의 변화등을 들 수 있고 이러한 변화는 치은의 염증과 탈회를 유발한다. 이에 본 연구는 한국인 고정식 교정장치 장착환자에서 장치장착 전후의 치태, 치은염 그리고 탈회의 변화를 시간에 따른 변화, 남녀간의 차이 그리고 좌.우 소구치 부위간의 차이를 통해 알아보고자 전신질환이 없고, 여자의 경우 초경이 지난 사람을 대상으로 대조군은 연세대학교 치과대학생 48명 (남자 26명, 여자 22명)과 실험군으로 고정식 교정장치로 치료할 환자 73명(남자 36명, 여자 37명)을 모두 잇솔질교육(TBI)을 실시한 후, 치태지수, 치은염지수 그리고 탈회 지수에 대하여 대조군은 3주 간격으로 2회를, 실험군은 최초측정을 하고 고정식 교정장치를 부착한 뒤 3주, 6주, 9주에 걸쳐 총 4회 측정을 실시하였다. 이상의 자료를 분석한 결과 다음과 같은 결론을 얻었다. 1. 치태지수(PI)는 고정식 교정장치 장착후 3주의 측정 이후 서서이 증가하였다. 2. 치은염지수(GI)는 고정식 교정장치 장착후 3주의 측정 이후 치태 지수의 증가보다 좀더 빠른 속도로 증가하였다. 3. 탈회는 3주와 6주 사이에서 발생하기 시작하며, 탈회지수(DI)는 처음과 비교하여 6주 측정시부터 증가하기 시작하였으나 통계적으로 유의한 차이를 발견할 수는 없었다. 4. 좌.우 소구치 부위의 비교에서는 실험군의 치태지수와 치은염지수에서 우측에서 높은 값을 보였다.우측간의 발생빈도는 구순열이 53.3:46.7, 구순치조열이 59.5:40.5, 구순구개열이 59.2:40.8 으로서 좌측의 발생빈도가 우측에 비해서 높았다. 3. 순구개열의 남:여 발생빈도는 구순열은 57.9:42.1, 구순치조열은 68.8:31.2, 구순구개열은 76.1:23.9 로서 남자의 발생빈도가 여자에 비해서 높았다. 그러나 구개열에서는 41.7:58.3으로서 여자의 발생빈도가 남자에 비해서 높게 나타났다. 4. 내원 환자를 연령군 별로 조사한 결과 7-12세 군이 $52\%$로서 압도적으로 많았고, 0-6세 군 ($20.4\%$), 13-18세 군($17.2\%$), 18세 이상 군 ($10.4\%$)의 순이었다. 5. 구순열의 봉합수술시기로는 0-3개월 군이 $60.3\%$로서 가장 많았고, 4-6개월 군이 $17.9\%$로 두 번째였다. 6. 구개열의 봉합수술시기로는 1-2세군이 $31.7\%$로 가장 많았고, 0-1세군은 $25.6\%$, 2-3세군이 $12.1\%$였다. 구개 및 상악 성장이 어느 정도 이루어진 5세 이상 군은 $11.6\%$를 차지하였다.7. 구순 반흔 제거수술시기로는 4-6세군 ($27.5\%$), 6-8세군 ($19.6\%$), 2-4세군 ($13.7\%$)이 $60\%$이상을 차지하여 초등학교 취학 전에 구순의 반흔을 제거하려