• 치위생과학회지
• /
• 제12권1호
• /
• pp.39-44
• /
• 2012

본 연구는 치면세균막 관리 프로그램에 의한 치면세균막 감소 효과 및 관리 난이도를 알아보기 위하여 충남지역에 위치한 N대학교 치위생학과에서 2006년부터 2011년까지 학생들이 제출한 임상치위생 증례보고서의 대상자 158명의 기록 가운데 가운데 본 연구에 적절하지 않은 기록을 제외하고 총 131부(82.9%)의 진료기록부를 최종분석에 사용하였다. 1. 치면세균막 관리 프로그램 수행 시 방문 차수에 따른 치면세균막 감소효과를 확인한 결과 2회 방문자(p=0.001), 3회, 4회, 5회, 6회 방문자(p<0.001)는 각각 방문 횟수가 많을수록 치면세균막 지수가 유의하게 감소하였다. 하지만 7회 방문한 대상자의 경우에는 감소된 정도가 통계적으로 유의하지 않았다(p=0.142). 2. 치면세균막 관리 프로그램의 최종방문 시 치아 부위별 치면세균막 지수 평균을 비교하기 위하여 치아 부위를 순/협면, 설/구개면, 인접면으로 분류했을 때에는 설면/구개면의 치면세균막 지수가 26.5%로 가장 높았다. 3. 상/하악의 치면세균막 지수를 측정한 결과 상악과 하악의 치면세균막 지수 간 유의한 차이가 나타났다(t=-2.501, p=0.014). 순/협면, 설/구개면, 인접면의 치면세균막 지수에 대한 분석 결과 순/협면, 설/구개면, 인접면의 치면세균막 지수는 유의한 차이를 보였다(Wilks의 람다=0.686, F=26.329, p<0.001). 육분악의 치면세균막 지수를 확인한 결과 각 부위별로 유의한 차이가 있는 것으로 나타났다(Pillai의 트레이스=0.166, F=4.443, p=0.001).

• 한국치위생학회지
• /
• 제23권4호
• /
• pp.227-234
• /
• 2023

Objectives: Cetylpyridinium chloride CPC-based mouthwashes are well known to have no harmful ingredients in the mouth and can be used for a long time. The purpose of this study was to evaluate the effect of using CPC-based mouthwashes to suppress the biofilm formation and antibiotics for handling orthodontic appliances. Methods: To measure the antibacterial effect, Streptococcus mutans (S. mutans) cultured orthodontic appliances were precipitated in Gargreen and Polident for 5 minutes, incubated at 37℃ for 24 hours(h). In order to measure the biofilm removal effect, the degree of biofilm formation on the orthodontic appliances was stained with a methylene blue and the difference before and after was compared using image J software program (NIH Image J; NIH, Bethesda, MD). Results: The viability of S. mutans according to the concentration showed that Gargreen and Polident inhibited colony formation compared to the control, respectively (p<0.01). The degree of biofilm formation was significantly higher in the control, however both Gargreen and Polident significantly reduced it compared to the before and after condition on removable orthodontic appliances (p<0.01). Conclusions: This study suggests that the use of Gargreen, a cetylpyridinium chloride based oral cleaning cleanser, could be replaced by Polident for antibacterial effect and biofilm formation on removable orthodontic appliances.

• Journal of Periodontal and Implant Science
• /
• 제49권5호
• /
• pp.319-329
• /
• 2019

Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.