• Journal of dental hygiene science
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• v.12 no.1
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• pp.39-44
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• 2012

For the purpose of examining the dental biofilm reduction effect and control difficulty level through a dental biofilm control program in this study, the total of 131 medical records (82.9%) were used for the final analysis upon excluding the records that were not suitable for this study among the records of 158 persons for the medical records of clinical hygiene case reports that were submitted from 2006 to 2011 by students at the Department of Dental Hygiene at N University located in Chungnam region. The result of examining the dental biofilm reduction effect according to the visit number when conducting a dental biofilm control program showed that the dental biofilm index reduced meaningfully as the visit number increased. However, in the case of those that visited for 7 sessions, the level of reduction was not statistically meaningful. For the purpose of comparing the dental biofilm index mean per area of teeth during the final session visit of dental biofilm control program, the area of teeth was classified into labial/buccal surface, lingual/palatal surface and proximal surface, and the dental biofilm index of lingual/palatal surface was the highest with 26.5%. The result of measuring the dental biofilm of maxilla/mandible revealed meaningful differences between the dental biofilm index of maxilla and mandible. The result of analyzing the dental biofilm index of labial/buccal surface, lingual/palatal surface and proximal surface revealed meaingful differences among the dental biofilm index of labial/ buccal surface, lingual/palatal surface and proximal surface.

• Journal of Korean society of Dental Hygiene
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• v.23 no.4
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• pp.227-234
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• 2023

Objectives: Cetylpyridinium chloride CPC-based mouthwashes are well known to have no harmful ingredients in the mouth and can be used for a long time. The purpose of this study was to evaluate the effect of using CPC-based mouthwashes to suppress the biofilm formation and antibiotics for handling orthodontic appliances. Methods: To measure the antibacterial effect, Streptococcus mutans (S. mutans) cultured orthodontic appliances were precipitated in Gargreen and Polident for 5 minutes, incubated at 37℃ for 24 hours(h). In order to measure the biofilm removal effect, the degree of biofilm formation on the orthodontic appliances was stained with a methylene blue and the difference before and after was compared using image J software program (NIH Image J; NIH, Bethesda, MD). Results: The viability of S. mutans according to the concentration showed that Gargreen and Polident inhibited colony formation compared to the control, respectively (p<0.01). The degree of biofilm formation was significantly higher in the control, however both Gargreen and Polident significantly reduced it compared to the before and after condition on removable orthodontic appliances (p<0.01). Conclusions: This study suggests that the use of Gargreen, a cetylpyridinium chloride based oral cleaning cleanser, could be replaced by Polident for antibacterial effect and biofilm formation on removable orthodontic appliances.

• Journal of Periodontal and Implant Science
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• v.49 no.5
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• pp.319-329
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• 2019

Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.