• Restorative Dentistry and Endodontics
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• 제13권2호
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• pp.307-322
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• 1988

The purpose of this study is to evaluate the pulpal responses to the base materials such as zinc oxide eugenol cement, calcium hydroxide, zinc phosphate cement, polycarboxylate cement and glass ionomer cement. The 100 caries free dog teeth were devided into 2 groups by remaining dentin thickness (Group A: 0.4-0.6 mm, Group B: 0.8-1.0 mm) and each group were devided into 5 subgroups. The intervals of observation period are 3days, 1 week, 2 weeks, 4 weeks and 8 weeks respectively after experiment. The specimens were fixed with 10% formalin and decalcifed in 5% nitric acid. All specimens were stained with Hematoxylin-Eosin and examined histopathologically. The results were as follows. 1. In group A, atropy or hyperplasia in odontoblasts were seen in zinc oxide eugenol cement, calcium hydroxide and zinc phosphate cement. No changes in odontoblasts were seen in polycarboxylate cement and glass ionomer cement. 2. In group A, increase of predentin were seen in all experimental materials. 3. In group A, vascular congestion were seen in all experimental materials and inflammation were seen on 3 days in zinc oxide eugenol cement, 8 weeks in zinc phosphate cement and hemorrage were seen on 3 days in zinc phosphate cement. 4. In group B, changes of odontoblasts were not seen all experimental materials. 5. In group B, increase of predentin and vascular congestion were seen in all experimental materials but inflammation were not seen.

• 대한소아치과학회지
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• 제24권4호
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• pp.776-780
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• 1997

Patient with alveolar abscess due to dental caries with severe alveolar bone loss, severe tooth mobility, root resorption need extraction of tooth because it is impossible to carry out pulp treatment and restoration by using conventional method. Early loss of primary molar might cause masticatory interference, extrusion of opposing tooth, problem in maintaining space and interference on eruption of permanent tooth. Especially, early loss of primary second molar before the eruption of permanent first molar might cause space closure by mesially erupted permanent first molar and impaction of second premolar. In such a case, distal shoe space maintainer and removable space regaining appliance was the first choice of treatment. But, distal shoe space maintainer need precise adaptation and might cause chronic inflammation if the oral hygiene is poor. In a case using removable space regaining appliance, patient's cooperation is most important. If the distal root of primary second molar is comparably sound and alveolar abscess with alveolar bone loss is localized at mesial root, hemisection should be carried out for precise guide to eruption of the permanent first molar, restoration of masticatory fuction and solution to the discomfort of the patient

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권6호
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• pp.593-598
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• 2008

Arsenic trioxide is one of the 'tooth pulp devitalizing agents' used through the dental history when anaesthesia was not available. But owing to its capacity to kill cells in surrounding tissues, the use of arsenic trioxide in vital pulpotomy has been reduced. Arsenic trioxide can cause necrosis of gingiva, bone which can cause osteomyelitis of the jaws. But some dentists still continue to use arsenic trioxide in their endodontic practices. The purpose of this article is to present arsenic trioxide induced osteomyelitis on maxilla and treatment process.

• 치과방사선
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• 제26권2호
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• pp.181-189
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• 1996

Ameloblastic odontoma is a mixed odontogenic tumor, which is characterized as being extremely rare, displaying aggressive clinical behavior, resembling a mixed radiopaque and radiolucent radiographic appearance and containing both ameloblastoma and a composite odontoma. Seven-year-old boy complaining of delayed eruption of the left lower permanent 1st molar came to the department of Oral & Maxillofacial Radiology at Chonbuk National University Hospital. The list of our radiographic differential diagnosis included the dental follicle, compound odontoma, complex odontoma and cystic odontoma. The microscopic analysis showed the lesion with the characteristics of an ameloblastic odontoma. The obtained results were as follows; 1. The area was asymptomatic, but the delayed eruption of the left lower permanent 1st molar was observed. Radiographically, well-defined widened pericoronal radiolucency containing several tooth-like radiopacities involving the impacted left lower permanent 1st molar and well-defined round radiolucency containing irregular radiopaque mass were observed. 3. Histopathologically, several tooth-like structures, odontoma components with the area of typical enamel, dentin and pulp, and ameloblastic components with typical follicular ameloblastoma were observed.

• Restorative Dentistry and Endodontics
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• 제6권1호
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• pp.87-92
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• 1980

생쥐 치수내 조상아세포의 미세구조연구 중에 종종 보게되는 원형섬모의 미세구조와 이들의 가능한 기능을 다음과 같이 추정하였다. 1. 상아질형성물질을 분비하는 기능을 가진 조상아세포내 2가지 다른 형태의 원형섬모가 발견된다. 2. 조상아세포내 2개의 Basal Centriole을 가지고 있으면서 아마 중앙에는 쌍으로 된 microtuble이 없고 쌍으로 된 9개의 말초섬유를 가진 형태의 원형섬모는 조상아세포와 치수내 말초신경 사이에 일어나는 지각 기능에 관여 하는 것으로 추측된다. 3. 다른 한형태의 섬모, 즉 한개의 Centriole을 가지고 있으면서 쌍으로된 9개의 말초섬유를 가진 섬모는 조상아세포에서 분비된 상아질 형성물질을 움직이게 하는 이동가능에 관여하는 것으로 사료된다.

• Restorative Dentistry and Endodontics
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• 제28권5호
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• pp.409-418
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• 2003

본 연구는 치수 염증 시 IL-8과 MCP-1 분비에서 neuropeptide의 역할에 대해 관찰하고자 발거된 건전한 치아를 수직 파절시켜 치수조직을 채취하여 배양된 치수세포 및 혈관내피세포(ECV 304세포)를 각기 다른 농도의 Substance P(SP)로 12시간 자극하였고, 24시간 동안 4시간 간격으로 시간대별로 자극하였으며, 또 치수세포를 Calcitonin gene-related peptide (CGRP)로 12시간 자극하였다. 이들 세포를 SP길항제 (Spantide)로 15분간 차단한 후 SP로 12시간 재 자극하였으며, SP와 CGRP혼합액을 12시간 자극하였다. 상기의 실험 후 부유물로 ELISA를 시행하여 IL-8과 MCP-1의 분비 량을 측정하였다. 치수세포는 SP로 자극 시 IL-8이 현저히 증가한 반면, CGRP는 효과가 없었으며, SP와 CGRP를 혼합자극 시 시너지 효과 또한 없었고, Spantide는 치수세포의 IL-8과 MCP-1의 분비를 차단시켰다. 치수세포를 SP로 24시간 동안 4시간 간격으로 자극 시 8시간 후 최대의 IL-8은 분비량 나타내었으며, 8시간과 12시간 사이에서 최대의 MCP-1 분비량을 나타내었다. ECV 304세포를 SP로 자극 시 IL-8과 MCP-1 분비량이 미약하게 증가하였으며, Spantide는 ECV 304세포의 IL-8과 MCP-1 분비를 억제시켰다.

• Restorative Dentistry and Endodontics
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• 제43권4호
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• pp.38.1-38.9
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• 2018

The restoration of endodontically treated teeth (ETT) with more than one cusp missing and thin remaining walls is challenging for the general practitioner. The use of posts combined with full coverage restorations is a well-established approach, yet not following the minimal invasive principles of adhesive dentistry. Endocrowns are indirect monoblock restorations that use the pulp chamber of the ETT for retention. In this study the fabrication of 4 endocrowns and their clinical performance will be discussed. Two clinical cases include computer-aided design/computer-aided manufacturing manufactured molar endocrowns (one feldspathic ceramic and one hybrid composite-ceramic restoration) and the other two are dental laboratory manufactured resin composite premolar endocrown restorations. The modified United States Public Health Service criteria were used to assess the clinical behavior of the restorations at different follow up periods. Endocrown restorations present a satisfactory clinical alternative, either by the use of resin composite or glass ceramic and hybrid materials. Specific guidelines with minimal alterations should be followed for an endocrown restoration to be successful. Due to limited evidence regarding the long term evaluation of this restorative technique, a careful selection of cases should be applied.

• Restorative Dentistry and Endodontics
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• 제46권3호
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• pp.46.1-46.9
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• 2021

The aim of this review was to evaluate the effects of different access cavity designs on endodontic treatment and tooth prognosis. Two independent reviewers conducted an unrestricted search of the relevant literature contained in the following electronic databases: PubMed, Science Direct, Scopus, Web of Science, and OpenGrey. The electronic search was supplemented by a manual search during the same time period. The reference lists of the articles that advanced to second-round screening were hand-searched to identify additional potential articles. Experts were also contacted in an effort to learn about possible unpublished or ongoing studies. The benefits of minimally invasive access (MIA) cavities are not yet fully supported by research data. There is no evidence that this approach can replace the traditional approach of straight-line access cavities. Guided endodontics is a new method for teeth with pulp canal calcification and apical infection, but there have been no cost-benefit investigations or time studies to verify these personal opinions. Although the purpose of MIA cavities is to reflect clinicians' interest in retaining a greater amount of the dental substance, traditional cavities are the safer method for effective instrument operation and the prevention of iatrogenic complications.

• Restorative Dentistry and Endodontics
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• 제46권3호
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• pp.37.1-37.11
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• 2021

Objectives: This systematic review evaluated the efficacy of the supplementary use of the XP-endo Finisher on bacteria content reduction in the root canal system. Materials and Methods: In-vitro studies evaluating the use of the XP-endo Finisher on bacteria content were searched in four databases in July 2020. Two authors independently screened the studies for eligibility. Data were extracted, and risk of bias was assessed. Data were meta-analyzed by using random-effects model to compare the effect of the supplementary use (experimental) or not (control) of the XP-endo Finisher on bacteria counting reduction, and results from different endodontic protocols were combined. Four studies met the inclusion criteria while 1 study was excluded from the meta-analysis due to its high risk of bias and outlier data. The 3 studies that made it to the meta-analysis had an unclear risk of bias for at least one criterion. Results: No heterogeneity was observed among the results of the studies included in the meta-analysis. The study excluded from the meta-analysis assessing the bacteria counting deep in the dentin demonstrated further bacteria reduction upon the use of the XP-endo Finisher. Conclusions: This systematic review found no evidence supporting the supplementary use of the XP-endo Finisher on further bacteria counting the reduction in the root canal.

• International Journal of Stem Cells
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• 제16권3호
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• pp.304-314
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• 2023

Background and Objectives: Ear cartilage malformations are commonly encountered problems in reconstructive surgery, since cartilage has low self-regenerating capacity. Malformations that impose psychological and social burden on one's life are currently treated using ear prosthesis, synthetic implants or autologous flaps from rib cartilage. These approaches are challenging because not only they request high surgical expertise, but also they lack flexibility and induce severe donor-site morbidity. Through the last decade, tissue engineering gained attention where it aims at regenerating human tissues or organs in order to restore normal functions. This technique consists of three main elements, cells, growth factors, and above all, a scaffold that supports cells and guides their behavior. Several studies have investigated different scaffolds prepared from both synthetic or natural materials and their effects on cellular differentiation and behavior. Methods and Results: In this study, we investigated a natural scaffold (alginate) as tridimensional hydrogel seeded with progenitors from different origins such as bone marrow, perichondrium and dental pulp. In contact with the scaffold, these cells remained viable and were able to differentiate into chondrocytes when cultured in vitro. Quantitative and qualitative results show the presence of different chondrogenic markers as well as elastic ones for the purpose of ear cartilage, upon different culture conditions. Conclusions: We confirmed that auricular perichondrial cells outperform other cells to produce chondrogenic tissue in normal oxygen levels and we report for the first time the effect of hypoxia on these cells. Our results provide updates for cartilage engineering for future clinical applications.