• 대한치과의사협회지
• /
• 제11권11호
• /
• pp.723-729
• /
• 1973

• Journal of Microbiology and Biotechnology
• /
• 제9권3호
• /
• pp.260-264
• /
• 1999

The combined activities of dextranase and amylase(DXAMase) from Lipomyces starkeyi KSM 22 produced from starch fermentation inhibited or prevented dental plaque formation. The activities were stable in commercial mouthwash products; DXAMase activity retained over 93％ of original activity after 6 months at 23℃. We examined the effects of enzyme inhibitors and active ingredients in mouthwash on DXAMase activity. The DXAMase was stable with 0.29％(w/v) EDTA, 20％ (v/v) ethanol, 0.05％ (w/v) fluoride, and 0.05％ (w/v) SDS. Among the active ingredients of mouthwash, sodium benzoate (up to 1 ％, w/v) had no inhibitory effect on either dextranase or amylase activity. In the case of cetylpyridinium chloride, the addition of 0.05％ (w/v) inhibited 6％ of dextranase activity and 13％ of amylase activity. Propylene glycol (up to 1％, w/v) showed no inhibitory effect on either enzyme activity. DXAMase (5 IU/㎖) in mouthwash could remove pre-formed films of glucan-bound S. mutans cells. The addition of 0.1 IU/㎖ DXAMase in mouthwash prevented the formation of insoluble-glucan. These in vitro properties of L. starkeyi KSM 22 DXAMase are desirable for its application as a dental plaque control agent.

• 미생물학회지
• /
• 제17권3호
• /
• pp.131-136
• /
• 1979

충치 sample 50개에서 46 strains의 Streptococcus sp.를 분리하여 그 생리적 특징을 조사한 바 1. 대부분의 균주들이 non-haemolytic strain이었으며, 2. 기질로 maltose, inulin, mannitol, sorbisI, lactose, mannabinose를 분해하여 산을 생성하고 arabinose와 xylose는 균주에 따라 차이를 보였으며, 3. Sucrose broth에서는 거의 gel을 형성하였다.

• 보건교육건강증진학회지
• /
• 제26권1호
• /
• pp.105-115
• /
• 2009

Objectives: This study was conducted to assess dental health states of disabled people and analyze association between perception and awareness toward dental health and dental health status. Methods: The survey was performed from June 25 through October 30, 2004. A total of 548 disabled people participated in the study with details of 419 living in eight residential care centers located in Gyeongsangbuk-do and 129 children from a special school and two day-care centers. All subjects underwent oral examination and surveyed through a questionnaire. Parents of 129 children with disabilities were also surveyed through a separate questionnaire. Results: The dental caries experience rate was 82.1% of total 548 subjects. By age, those in their 20s experienced a high rate of dental caries with 87.5%. By educational level, those with a middle school education experienced a high dental caries rate with 91.8% (p<0.05). Of total subjects who experienced dental caries, 78.9% had experience in dental caries treatment. By age, those in their 10s showed a high rate of dental caries treatment with 87.4%(p<0.05). By educational level, those with a high school education showed a high rate of dental caries treatment with 87.7%(p<0.05). Those in residential care centers had a high rate of dental caries treatment with 82.1%, which is significantly higher than 68.8% of those who used day-care centers. A tooth extraction rate was 38.0% of total subjects. Those in their 40s had a higher rate of tooth extraction(p<0.01). Those in residental care centers had a significantly higher rate of extraction with 43.4%, compared with 20.2% of those in day-care centers. Of total subjects, 61.5% had plaque. A high rate of plaque formation was observed in those in their 40s(92.0%), those with a high school education(84.0%) and those with multiple disabilities(77.8%)(p<0.01). Among total subjects, 47.6% maintained healthy periodontal tissue. Those in their 40s and those with multiple disabilities had diseased periodontal tissue(p<0.01). Of 129 disabled children, 43.8% had plaque with parents who were not oral health-conscious while 18.6% had plaque with parents who were oral health-conscious, showing a significant difference(p<0.05) Conclusion: The results of the study suggest the need for educating parents with disabled children about oral health and strengthening programs for oral health for teachers working at special schools and day-care centers.

• 치위생과학회지
• /
• 제15권4호
• /
• pp.437-444
• /
• 2015

본 연구는 시판중인 국내외 제조 플레인 탄산수 5종을 선정하여 우치 법랑질 침식 및 그에 따른 세균 침착 정도를 확인하였다. 탄산수에 시편을 침지 후 용출된 칼슘 농도를 측정하고, SEM을 이용하여 법랑질 표면의 침식을 관찰하였다. 이 후 S. mutans 균과 S. sorbinus 균이 접종된 배지에 시편을 함께 배양하여 침식된 시편의 세균 침착 정도를 확인하여 다음과 같은 결론을 얻었다. 실험에 사용된 탄산수 5종은 수소이온농도가 pH 5.5보다 낮았고, 치아의 탄산수 침지 후 칼슘의 용출이 확인되었다. SEM을 이용한 치면 침식 분석 결과, 칼슘 용출은 탄산수에 의한 법랑질 침식이 원인임을 확인하였고, 두 분석 방법을 통해 인공 탄산수와 광천수간의 뚜렷한 침식 활성 차이는 관찰되지 않았다. 또한 탄산수에 의해 유발된 치아 침식으로 치면 세균 침착이 용이해지는 것으로 확인되었다. 이런 결과들로부터 탄산수 음용 시 치아 침식이 유발될 수 있으며 그에 따른 구강 내 세균 침착이 가중될 수 있으므로, 탄산수 음용 시 치아 침식 가능성에 대한 소비자의 인식이 필요하며, 탄산수를 물 대체음료 정도로 생각하기보다는 무가당 콜라와 같은 탄산음료에 준하는 범주로 포괄하여 음용하는 것이 바람직하다고 생각된다.

• Journal of Periodontal and Implant Science
• /
• 제51권4호
• /
• pp.276-284
• /
• 2021

Purpose: The aim of this randomized clinical trial was to assess whether chemical cleansing using a sulfonic/sulfuric acid gel solution (HBX) as an adjunct to scaling and root planing (SRP) resulted in a decrease in residual plaque and calculus in deep periodontal pockets compared to SRP alone. Methods: Fifty-six patients with 56 hopeless posterior teeth, scheduled for extraction due to severe periodontitis, were enrolled in this study. Each tooth was randomly assigned to 1 of the 2 experimental procedures. The test teeth were subjected to the irrigation of the subgingival area with HBX for 2 minutes, followed by SRP with hand and ultrasonic instruments for 14 minutes, and then extracted. The control teeth received only mechanical instrumentation before extraction. Residual biofilm was evaluated on photographs and measured as total area and percentage of root surface covered by remaining plaque (RP) or calculus (RC) after treatment. Results: The initial pocket depth (PD) and total subgingival root surface area were similar between the 2 treatment groups. After treatment, the total subgingival root area covered by RP and RC was statistically significantly larger (P<0.001) in the control group than in the test group. The test teeth showed a lower percentage of RP, but a higher percentage of RC than the control teeth (both P<0.001). Complete calculus removal was achieved in 42% of the control teeth surfaces and in 25% of the test teeth surfaces for a PD of 4 mm. Conclusions: The additional chemical cleansing with HBX resulted in a statistically significant improvement in bacterial plaque removal during SRP of deep pockets, but it was not effective in reducing calculus deposits.

• 대한치과의사협회지
• /
• 제58권3호
• /
• pp.140-151
• /
• 2020

Purpose: To evaluate reliability of Q-ray view (Aiobio Inc,. Seoul, Korea) for assessing retention status of pit and fissure sealants. Methods: Pit and fissure sealants of 58 permanent molars from 15 third-grade students were examined. Posterior teeth with ≥1 pit and fissure sealants applied to the occlusal surface for >6 months were examined. The teeth were examined using traditional visual-tactile assessments and combined Q-ray view. Pit and fissure sealants were evaluated by assessing marginal plaque, marginal discoloration, marginal integrity, retention, and presence of caries. Fleiss kappa and Cohen's kappa values were calculated to compare inter- and intrarater agreements between visual-tactile and combined Q-ray view assessments. Results: Regarding interrater agreement in visual-tactile assessments, K values of Cohen's kappa for marginal plaque, marginal discoloration, and presence of caries were 0.22-0.57, 0.36-0.57, and 0.43-0.61, respectively, and agreements ranged from slight to moderate. When combined with Q-ray view, the values were 0.81-0.89, 0.69-0.88, and 0.80-0.90, respectively, and agreements ranged from substantial to nearly perfect level, indicating statistical significance. Marginal plaque (0.81-0.83), marginal discoloration (0.57-0.89), and presence of caries (0.69-0.91) showed higher agreements in combined Q-ray view than in visual-tactile assessments, and kappa values of marginal plaques were significantly higher in combined Q-ray view than in visual-tactile assessments. Conclusion: Evaluating retention status of pit and fissure sealants using Q-ray view showed higher reliability than using visual/tactile assessments for marginal plaque, marginal discoloration, and presence of caries. Therefore, Q-ray view may be used to assess the retention status of pit and fissure sealants.

• 대한소아치과학회지
• /
• 제25권3호
• /
• pp.619-626
• /
• 1998

The critical etiologic factor in the development of dental caries is dental plaque. The main component of dental plaque is the mutan produced by Streptococcus mutans. The following results were obtained by using blue mutan to assess the factors affecting the mutan-digesting activity of Micromonospora aurantiaca isolated from oral cavity. Micromonospora aurantiaca digested more blue mutan in the minimal essential broth at pH 7.0 than at pH 5.5 or 8.5, and at $37^{\circ}C$ than at $32^{\circ}C\;or\;42^{\circ}C$. Blue mutan was similarly digested at the range of 1mM to 16mM of $CaCl_2$ and 0.1mM to 6.4 mM of $MgCl_2$, while being significantly digested at the concentration of 2.5mM of KCl. When the concentration of glucose was decreased in the minimal essential broth, the digestion of blue mutan was increased. When the culture supernatant of Micromonospora aurantiaca in the RL broth with 1% glucose or 0.5% mutan was mixed with 2 ${\times}$ BHIYS broth containing 0.5% yeast extract and 10% sucrose, the formation of artificial plaque on the orthodontic wires by Streptococcus mutans was inhibited(p<0.05). These results indicated that the production of mutanase was identified in the culture supernatant of Micromonospora aurantiaca, suppressing the formation of artificial plaque by Streptococcus mutans.

• 대한치과교정학회지
• /
• 제40권2호
• /
• pp.95-105
• /
• 2010

교정치료 중 구강 내 환경 변화를 파악하기 위하여 교정치료 시작 후 24개월까지의 임상적 구강위생지수 및 타액, 구강 내 미생물의 변화양상을 평가하였다. 30명의 환자를 대상으로 하여, 임상적 구강위생지수(DMFS [decayed, missing and filled surfaces], O'Leary's plaque index, plaque pH), 타액의 변화(비자극/자극시의 타액량, 완충능(buffer capacity), pH, 잠혈(occult blood)류량, 구강 내 미생물(Streptococcus mutans, Lactobacillus) 변화를 파악하였다. 추가적으로 설문지를 이용하여 구강위생관리 및 식습관을 평가하였다. 분산분석, 회귀분석 및 Spearman의 상관분석을 통해 데이터를 분석하였다. 교정치료 도중 DMFS 값은 유의성있게 증가한 반면, plaque index는 감소하였고, plaque pH는 산성을 나타내었다 (p = 0.23). 비자극 시 타액량은 치료 중 유의성있게 변화하였으며 (p = 0.13), 자극 시의 타액량은 여성에서 장치 부착 이후 증가하는 양상을 보였다. Buffer capacity는 남성에서 치료 도중 감소되었으며, 타액의 pH는 유지되었다. 구강 내 미생물 및 잠혈류량은 고위험 수준까지 증가하였으며 성별의 차이는 나타나지 않았다 (p > 0.05). 치료 전 plaque와 구강위생용품의 사용 (r = 0.429; p = 0.018), 최종 DMFS와 비자극 시의 타액량 (r = -0.372; p = 0.043) 간에 뚜렷한 상관관계가 확인되었다. 교정장치의 부착으로 인해 구강 내 미생물은 증가하고, plaque pH는 산성화되어 치주조직의 손상이 일어나게 되었다. 또한 Buffer capacity는 변하지만, 치료 기간중의 타액의 pH는 유지되었다.

• Journal of Oral Medicine and Pain
• /
• 제40권4호
• /
• pp.135-139
• /
• 2015

Desquamative gingivitis (DG) is a gingival manifestation of systemic mucocutaneous disorders such as mucous membrane pemphigoid, oral lichen planus, and pemphigus vulgaris. The lesion is very painful, so affects the patient's ability to do proper oral hygiene practices. This may be a potential risk factor for long-term periodontal health. However, there is some controversy about the relationship between the existence of DG and periodontal status. Although the correlation between DG-associated diseases and periodontal status is not to be certain, early diagnosis and appropriate treatment including adequate plaque control and removal of local factors is very important for preventing the progression of diseases and destruction of periodontal tissues.