• 한국치위생학회지
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• 제13권5호
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• pp.889-900
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• 2013

Objectives : Molecular biology techniques were employed to assess diversity of bacterial in children's dental caries. Methods : DNA of germs was extracted and the diversity of the 16S rRNA clones was analyzed by amplified rDNA restriction analysis and sequencing. The experimental samples were pit and fissure caries (PC), deep dentinal caries (DC), smooth surface caries (SC), and supragingival plaque (PQ) from 50 children of age less than 12 years old. The control group was healthy teeth supragingival plaque (HT). Thirty clones from each 16S rRNA clone library of 5 samples were randomly selected, thus a total of 150 clones were analyzed. Results : Amplified rDNA restriction analysis uncovered 18, 20, 11, 17, and 22 phylotypes from healthy teeth, pit and fissure caries, deep dentinal caries, smooth surface caries, and supragingival plaque, respectively. Sequencing analysis found the dominance of Actinomycs naeslundii and Fusobacterium nucleatum in the healthy teeth; Leptotrichia sp. in the pit and fissure caries; Actinomyces sp., Streptococcus mutans, and Rahnella aquatilis in the deep dentinal caries; Streptococcus mutans and Actinomyces sp. in the smooth surface caries; Enterobacter hormaechei and Streptococcus sanguinis in the supragingival plaque. Conclusions : Clonal analysis identified 6 phyla, 20 genera, and 51 species.

• Journal of Periodontal and Implant Science
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• 제30권4호
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• pp.815-821
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• 2000

The purpose of this study was to evaluate the influence of accessibility to dental cervices of maxillary molars upon plaque control level of these areas. Fifthy-seven dental students with healthy gingiae participated in this study. Maxillary dental casts were fabricated for each participants. Using the casts, cervical accessibility was measured at the mid-palatal point of maxillary first and second molars. Cervical accessibility was defined as the perpendicular distance from the entrance of gingival sulcus to the imaginary line between the most protruded points of palatal gingiva and tooth surface, and classified into degree I(${\leq}0.5mm$), II($>0.5mm,\;{\leq}1.0mm$), III($>1.0mm,\;{\leq}1.5mm$), and IV(>1.5mm). Plaque score was recorded as the distance from crest of gingival margin to the most coronal extent of plaque. Measurements of plaque score were repeated 3 times at 1-week intervals. After the baseline measurements, the participants began to use unitufted brushes on randomly assigned right or left side. Two weeks later, a session of plaque score records identical to the baseline measurements was started. The maxillary second molars showed higher cervical accessibility than the first molars(p<0.01), but the plaque scores of maxillary second molars were also higher than those of first molars(p<0.01). For the maxillary first molars, correlation between accessibility and plaque score was statistically significant, but such correlation was not found for the second molars. Use of unitufted brushes decreased the plaque score(p<0.01). Correlation between accessibility and the degree of plaque score improvement was not found. These findings suggest that cervical accessibility may influence the amount of plaque, and use of adjunctive oral hygiene devices may be helpful in maintaining optimal oral hygiene level at the areas of low cervical accessib ility.

• 한국치위생학회지
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• 제2권2호
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• pp.201-213
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• 2002

The closely related species Actinobacillus actinomycetemcomitans, Hemophilus aphrophilus and Hemophilus paraphrophilus are common findings in oral microbiota. The aims of this study were to compare the distribution of three species in healthy subjects and periodontitis patients using PCR for 16s rRNA gene. The DNA was extracted from the subgingival plaque and saliva in 122 subjects for restriction enzyme analysis with Hinf I and Hha I. In case of periodontally healthy person, A. actinomycetemcomitans was predominant than H. paraphrophilus in saliva sample, but H. paraphrophilus was predominant than A. actinomycetemcomitans in subgingival plaque sample. On the contrary, in case of periodontitis patients, H. paraphrophilus was predominant than A. actinomycetemcomitans in saliva sample, but A. actinomycetemcomitans was predominant than H. paraphrophilus in subgingival plaque sample. In addition, the fact was confirmed that the distribution of A. actinomycetemcomitns of women periodontitis patients was somewhat higher than men periodontitis patients in saliva and subgingival plaque samples. We convinced that the PCR method for 16s rRNA gene was important for screening and monitoring of periodontal disease.

• 디지털융복합연구
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• 제16권12호
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• pp.505-510
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• 2018

본 연구에서는 일반 칫솔과 씹는 칫솔의 치면세균막 제거효과를 비교하고 이를 통해 현재 출시된 씹는 칫솔이 일반 칫솔의 대용품으로 사용할만한 실용성을 갖추고 있는지와 실생활에서의 활용도 여부 등을 알아보고자 하였다. 연구 대상자는 전신 질환이 없으며 잔존치아 16개 이상 존재하는 구강상태가 양호한 20대의 건강한 성인 30명을 대상으로 하였으며, 일반칫솔과 씹는 칫솔 사용 후 치면세균막지수 감소율을 계산하였다. 일반 칫솔과 씹는 칫솔의 사용 전 후 치면세균막 감소효과를 비교한 결과, 일반 칫솔의 경우 PHP index는 7.02에서 1.91로 감소하여 치면세균막이 72.79% 감소한 결과를 나타내었으며, 씹는 칫솔은 6.72에서 4.08로 감소하여 치면세균막이 39.29% 감소한 결과를 나타내었다. 현재 국내에서는 다양한 구강관리용품이 출시되어 판매되고 있으며 일반인들의 올바른 구강관리용품 선택을 위하여 구강관리용품에 대한 다양한 연구가 필요할 것으로 생각된다.

• Journal of Periodontal and Implant Science
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• 제34권1호
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• pp.195-204
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• 2004

A novel glucanhydrolase from Lipomyces starkeyi KSM 22 has been suggested as a promising anti-plaque agent because it has been shown to have additional amylase activity and mutanase activity besides dextranase activity and to strongly bind to hydroxyapatite. Mouthrinsing with Lipomyces starkeyi KSM 22 glucanhydrolase solution was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were less frequent and less intense in human experimental gingivitis. In this study, Lipomyces starkeyi KSM 22 glucanhydrolase mouthrinses (1 and 2 unit/ml) were compared with a control mouthrinse (commercial 0.01% benzethonium chloride mouthrinse, $Caregargle^{(R)}$, Hanmi Pharmaceuticals) in the ability to inhibit plaque formation. A 3-replicate clinical trial using 4-day plaque regrowth model was used. Fifteen volunteers were rendered plaque-free on the 1st day of each study period, ceased toothcleansing, and rinsed 2X daily with allocated mouthrinse thereafter. On day 5, plaque accumulation was scored and the washout periods was 9 days for the next trial. Lipomyces starkeyi KSM22 glucanhydrolase(1 unit and 2 unit)- containing mouthrinse resulted in Significantly lower plaque formation in plaque area and thickness, compared to the control mouthrinse. There was no significant difference in plaque inhibition between enzyme-mouthrinses at 2 different concentrations used. This glucanhydrolase- containing mouthwash resulted in significantly lower plaque area severity index score and tended to have lower plaque thickness severity index score than those of control mouthrinse. But there was no significant difference according to the enzyme concentration. From these results, Lipomyces starkeyi KSM 22 glucanhydrolase-containing benzethonium chloride mouthrinse has greater anti-plaque effect than the commercial mouthrinse alone. Therefore this glucanhydrolase preparation is a promising agent for new mouthwash formulation in the near future.

• Journal of Periodontal and Implant Science
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• 제31권2호
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• pp.401-420
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• 2001

A novel glucanhydrolase from a mutant of Lipomyces starkeyi(KSM 22)has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and Lipomyces starkeyi KSM 22 dextranase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 dextranase are desirable for its application as a dental plaque control agent. This study was performed to determine oral hygiene benefits and safety of dextranase(Lipomyces starkeyi KSM 22 dextranase)-containing mouthwash in human experimental gingivitis. This 3-week clinical trial was placebo-controlled double-blind design evaluating 1U/ml dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 2 and 3 weeks, subjects were scored for plaque(Silness and $L{\ddot{o}e$ plaque index and plaque severity index), gingivitis($L{\ddot{o}e$ and Silness gingival index), and at baseline and 3 weeks of experiment, subjects were scored for plaque(Turesky-Quingley-Hein's plaque index and plaque severity index), tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice dailywithout toothbrushing. All the groups showed significant increase in plaque accumulation since 1 week of experiment. During 3 weeks' period, the dextranase group showed the least increase in plaque accumulation of Silness and $L{\ddot{o}e$ plaque index, compared to the chlorhexidine and placebo groups, but chlorhexidine group showed the least increase inplaque accumulation of Turesky-Quingley-Hein's plaque index. As for gingival inflammation, all the groups showed significant increase during 3 weeks of experiment. The dextranase group also showed the least increase in gingival index score, compared to the chlorhexidine as well as the placebo groups. Whereas the tooth stain was increased significantly in the chlorhexidine group, compared to the baseline score and the placebo group since 3 weeks of mouthrinsing. It was significantly increased after 3 weeks in the dextranase group, still less severe than the chlorhexidine group. As for the oral side effect, the dextranase group showed less tongue accumulation, bad taste, compared to the chlorhexidine group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwashin inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, in human experimental gingivitis. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.

• 대한소아치과학회지
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• 제26권1호
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• pp.14-24
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• 1999

The purpose of study was to develop new dental plaque disclosants which could replace erythrosine. Three food coloring agents(Red No.40, Blue No.1, and Mixed Green), erythrosine and fluorescein were tested for their color difference, antibacterial property, and biocompatibility. Color difference of Red No.40 was greater than that of erythrosine as concentration of solution increased. Color differences of Blue No.1 and Mixed Green were smaller than that of red dyes. Erythrosine showed obvious antibacterial property, but food coloring agents showed almost no antibacterial property. The taste and sensation of erythrosine was the worst, and the taste of Red No.40 and the sensation of Mixed Green were the most tolerable. Erythrosine stained dental plaque and oral soft tissue most deeply and long, and Blue No.1 was the next in the depth and longevity of stain.

• 대한치과의사협회지
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• 제55권2호
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• pp.172-179
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• 2017

Since early model of powered toothbrush (Broxadent) was introduced in the 1960s, the design of powered toothbrush has changed significantly. Today powered toothbrushes can be categorized as mechanical and sonic. The main patterns of movements in the modern powered toothbrushes are oscillation, reciprocation, and rotational. Powered brushes especially helpful for people who have disabilities or dexterity issue. These brushes are also highly recommended for patients who require a larger handle because these are easier to grasp. By systematic review of Cochrane group in 2014, powered toothbrushes reduced dental plaque and gingivitis more than manual toothbrushing in the short and long term. Now we can recommend powered toothbrushes to people who need to enhance self-control dental plaque removal efficacy.

• 한국치위생학회지
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• 제1권1호
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• pp.89-99
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• 2001

Estimating by clinical index the efficacy of three cases, where chewing gum is mixed with nanoparticle containing chlorhexidine, where chewing gum doesn't contain neither of them, and where with chlorhexidine only, this study has come to the following conclusion. 1. The chewing gum with chlorhexidine and chlorhexidine nanoparticle has shown more reduction of plaque index and gingival index than that with no chlorhexidine. 2. There could be seen a difference between the gum with chlorhexidine and the gum with chlorhexidine nanoparticle. 3. The gum with chlorhexidine nanoparticle has shown less level of plaque index than that chlorhexidine, which difference was only slight. 4. The gum with chlorhexidine nanoparticle wasn't absorved any tooth coloring. To put these results together, it is proposed that mixing chewing gum with chlorhexidine nanoparticle can be an efficient application.

• 대한치과의사협회지
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• 제9권12호
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• pp.812-818
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• 1971

The present investigation describes the chemical nature of purified extracellular polysaccharides synthesized by tow resembling streptococci, Streptococcus salivarius strain SD-1 and Streptococcus mitis strain SD-9, which was isolated from human dental plaque, with the following results. 1. Extracellular polysaccharide by Streptoccus salivarius strain SD-1 is recovered mostly in Fraction I-and II-45% of the presne purification procedure, leaving trace amount in Fraction I-and II-70%. 2. The dextran is the major polysaccharides in Fraction I-and II-45% of both strains with minor amounts of levan. 3. The Fraction I-and II-45% of both strains contain glucose and fructose, which its 70% of the same fractions glucose only. 4. It appears that the glycerol was the major end product of the Smith degradation of the Fraction I-45% of both strains.