• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.836-843
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• 2004

Nontypeable H. influenzae (NTHi), a Gram-negative obligate human pathogen, causes pneumonia, chronic bronchitis, and otitis media, and the respiratory epithelium is the first line of defense that copes with the pathogen. In an effort to identify transcriptional responses of human respiratory epithelial cells to infection with NTHi, we examined its differential gene expression using high density cDNA microarrays. BEAS-2B human bronchial epithelial cells were exposed to NTHi for 3 hand 24 h, and the alteration of mRNA expression was analyzed using microarrays consisting of 8,170 human cDNA clones. The results indicated that approximately 2.6% of the genes present on the microarrays increased in expression over 2-fold and 3.8% of the genes decreased during the 24-h infection period. Upregulated genes included cytokines (granulocyte-macrophage colony stimulating factor 2, granulocyte chemotactic protein 2, IL-6, IL-10, IL-8), transcription factors (Kruppel-like factor 7, CCAAT/enhancer binding protein $\beta$, E2F-1, NF-$\kappa$B, cell surface molecules (CD74, ICAM-1, ICAM-2, HLA class I), as well as those involved in signal transduction and cellular transport. Selected genes were further confirmed by reverse-transcription-PCR. These data expand our knowledge of host cellular responses during NTHi infection and should provide a molecular basis for the study of host-NTHi interaction.

• Molecules and Cells
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• 제25권4호
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• pp.538-544
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• 2008

Activity-dependent local translation in the dendrites of brain neurons plays an important role in the synapse-specific provision of proteins necessary for strengthening synaptic connections. In this study we carried out combined fluorescence in situ hybridization (FISH) and immunocytochemistry (IC) and showed that more than half of the eukaryotic elongation factor 1A (eEF1A) mRNA clusters overlapped with or were immediately adjacent to clusters of PSD-95, a postsynaptic marker, in the dendrites of cultured rat hippocampal neurons. Treatment of the neurons with KCl increased the density of the dendritic eEF1A mRNA clusters more than two-fold. FISH combined with IC revealed that the KCl treatment increased the density of eEF1A mRNA clusters that overlapped with or were immediately adjacent to PSD-95 clusters. These results indicate that KCl treatment increases both the density of eEF1A mRNA clusters and their synaptic association in dendrites of cultured neurons.

• 한국전산구조공학회논문집
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• 제22권4호
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• pp.349-354
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• 2009

초고층 건물의 안전성 및 사용성을 합리적으로 확보하고 유지하기 위해서 부재의 구조 반응 모니터링은 필요하다. 이러한 대형 건물의 건전도 모니터링은 최근 들어 많은 연구자들에 의해서 관심 되어지고 있지만 건물 특성상 계측 대상 부재가 많고 하중과 구조반응의 관계가 복잡하기 때문에 센싱 부재 선정의 어려움으로 그 적용에 한계가 있다. 본 논문에서는 부재의 변위기여도 및 변형에너지밀도를 기반으로 초고층 건물의 안전성 모니터링을 위한 구조반응 계측 부재 선정에 대한 연구를 수행하였다.

• 한국산학기술학회논문지
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• 제17권11호
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• pp.629-634
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• 2016

본 연구에서는 철도차량 내장재 화재성능평가기준 중 연기밀도 측정에서 주요 인자들의 영향을 합성고무바닥재를 이용하여 분석 및 검토하였다. 밀폐된 공간에서 운영되는 철도차량의 특성 상, 객실 내부에서 화재 발생 시에 연기로 인해 막대한 인명피해가 발생할 수 있다. 따라서 국내 외의 철도운영국가에서는 내장재의 연기 발생량을 엄격하게 규제하고 있으며, 이를 위해 내장재의 화재성능평가에 있어 연기밀도의 정확한 측정이 요구된다. 연기밀도의 측정에 있어 주요 영향인자로 시료 고유의 균질도, 장비를 이용한 조건 및 측정의 편차 그리고 장비를 운용 관리하는 환경의 영향을 꼽을 수 있다. 각 인자별 연기밀도에 영향도를 확인하기 위하여 불확도 추정에 따른 기여율 분석을 수행하였다. 그 결과, 광센서(PM tube)를 이용한 투과율 측정편차의 기여율이 약 86 %로 연기밀도 측정의 대표 영향인자임을 확인할 수 있었다. 그리고 높은 균질도를 가지는 시료를 대상으로 한 연기밀도 측정에서는 그 외 영향인자(시료연소용 복사열 조건(${\pm}0.5 kW/m^2$) 및 장비의 운용 관리환경)의 기여율이 약 11 %로 낮음을 확인할 수 있었다.

• Design & Manufacturing
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• 제13권2호
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• pp.37-43
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• 2019

This study conducted a research as to condensation heat transfer friction loss headby using three types of flat micro multi-channel tubes with different processing of micro-fin and number of channels inside the pipes and different sizes of appearances. In addition, identical studies were conducted by using smoothing circular tubes with 5mm external diameter to study heat enhancement factor and pressure drop penalty factor. 1) The friction head loss showed an increase as the vapor quality and mass flux increased. In case of saturation temperature, it shows an increase as it gets lower. These factors are the reason occurring as the lower the saturation temperature is, the higher the density of refrigerant vapor gets. The influence of heat flux is similar as the dryness is low, but as it gets higher, it lowers in heat flux, and as the high temperature of high heat flux, it is a factor that occurs as the density gets lower. 2) RMS error of the in case of friction head loss, it showed to be predicted as 0.45~0.67 by Chisholm, Friedel, Lockhart and Martinelli. 3) As forfriction head loss penalty factor, the smaller the aspect ratio is, the larger the penalty factor gets, and as for the effect of micro-fin, the penalty factor increased because it decreases to the gas fluid the way groove for the refrigerant's flow.

• 한국소음진동공학회논문집
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• 제14권5호
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• pp.370-376
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• 2004

The overall aim of this paper is to determine coupling loss factor of welding point between shell and cylinder using loss factor and structural loss factor. For this purpose, two kinds of loss factor were adopted. One is loss factor of each sub structure, another is structural loss factor based on the complex welded or assembled structure. Using these two parameters, it ispossible to derive the coupling loss factor which represent characteristic condition of SEA theory. Coupling loss factor of conjunction in complex structure was expressed as power balance equation. The derived equation for a coupling loss factor has been simplified on the assumption of one way (uni-directional) power flow between multi-sub structures. Using these conditions, it is possible to find the equation of coupling loss factor expressed as above two loss factors. To check the effectiveness of above equation, this paper used two-stage application. The first approach was application between simple cylinder and shell. The next was adopted rotary compressor. Rotary compressor has three main conjunctions between shell and internal vibration part. This equation was applied to find out the optimum welding point with respect to reduce the noise propagation. It shows the effective tool to evaluate the coupling loss factor in complex structure

• Communications for Statistical Applications and Methods
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• 제19권3호
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• pp.495-500
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• 2012

We consider a Bayesian test of independence in a two-way contingency table that has some zero cells. To do this, we take a three-stage hierarchical Bayesian model under each hypothesis. For prior, we use Dirichlet density to model the marginal cell and each cell probabilities. Our method does not require complicated computation such as a Metropolis-Hastings algorithm to draw samples from each posterior density of parameters. We draw samples using a Gibbs sampler with a grid method. For complicated posterior formulas, we apply the Monte-Carlo integration and the sampling important resampling algorithm. We compare the values of the Bayes factor with the results of a chi-square test and the likelihood ratio test.

• 대한화학회지
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• 제56권1호
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• pp.20-27
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• 2012

Complex permittivity spectra of Allyl Chloride (AC), 2-Butanone (2-BU) and their binary mixtures over the entire range of concentration were obtained using the Time Domain Reflectometry (TDR) technique in microwave frequency range at various temperatures. Static dielectric constant and relaxation time are obtained from complex permittivity spectra. Density ($\rho$) and refractive index ($n_D$) are also measured. These parameters are used to determine excess dielectric constant, excess inverse relaxation time, excess molar volume, excess molar refraction, polarity, Bruggeman factor and thermodynamic parameters viz. enthalpy of activation and entropy of activation. The values of static dielectric constant and relaxation time increases while density and refractive index decreases with the percentage of 2-Butanone in Allyl Chloride increases. Excess parameters were fitted to a Redlich-Kister equation.

• 물과 미래
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• 제25권3호
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• pp.79-86
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• 1992

ARF(Areal Reduction Factor) have been developed and used to convert point I-D-F to areal I-D-F in many countries. In Korea, through ARF was calculated in Han river basin by several researchers, it has limit to apply to other regions \ulcorner 새 low density of rainfall gauge station and shortage of data. In this study ARF has developed in areas of high density of rainfall gauge station, Pyungchang river(han river), Wi stream(nakdong river), and Bochung stream(Guem river) basin by fixed-area method. And coefficient of variation of annual mean precipitation was presented to use ARF in othere areas and its applicability was analyzed.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1999년도 가을 학술발표회 논문집
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• pp.147-152
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• 1999

Distribution of fracture system is an important factor to analyse instability of jointed rock slope. In the most case of rock slopes, joint distribution properties are related to potential, shape, size and locality of slope failure. The purpose of this paper is to present an application of fracture characterization related to rock slope failure. Fracture data used in this study are collected by scanline survey. Two aspects of fracture characterization for rock slope are handled in this study First, In order to determine the potential and shape of slope failure, trace length of joints is considered as the weighting factor about collected orientation data. Second, Relationship between trace length and spacing is analysed to estimate failure location and size. The distribution of fracture system is directly influenced on wedge failure. It is effective to analyse the orientation of fractures by using weighting factors associated with the trace length of fractures rather than to analyse only that of fractures. It gives a conclusion that the wedge failure occurred along the peak of fracture density(or intensity) cycles.