• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.836-843
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• 2004

Nontypeable H. influenzae (NTHi), a Gram-negative obligate human pathogen, causes pneumonia, chronic bronchitis, and otitis media, and the respiratory epithelium is the first line of defense that copes with the pathogen. In an effort to identify transcriptional responses of human respiratory epithelial cells to infection with NTHi, we examined its differential gene expression using high density cDNA microarrays. BEAS-2B human bronchial epithelial cells were exposed to NTHi for 3 hand 24 h, and the alteration of mRNA expression was analyzed using microarrays consisting of 8,170 human cDNA clones. The results indicated that approximately 2.6% of the genes present on the microarrays increased in expression over 2-fold and 3.8% of the genes decreased during the 24-h infection period. Upregulated genes included cytokines (granulocyte-macrophage colony stimulating factor 2, granulocyte chemotactic protein 2, IL-6, IL-10, IL-8), transcription factors (Kruppel-like factor 7, CCAAT/enhancer binding protein $\beta$, E2F-1, NF-$\kappa$B, cell surface molecules (CD74, ICAM-1, ICAM-2, HLA class I), as well as those involved in signal transduction and cellular transport. Selected genes were further confirmed by reverse-transcription-PCR. These data expand our knowledge of host cellular responses during NTHi infection and should provide a molecular basis for the study of host-NTHi interaction.

• Molecules and Cells
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• v.25 no.4
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• pp.538-544
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• 2008

Activity-dependent local translation in the dendrites of brain neurons plays an important role in the synapse-specific provision of proteins necessary for strengthening synaptic connections. In this study we carried out combined fluorescence in situ hybridization (FISH) and immunocytochemistry (IC) and showed that more than half of the eukaryotic elongation factor 1A (eEF1A) mRNA clusters overlapped with or were immediately adjacent to clusters of PSD-95, a postsynaptic marker, in the dendrites of cultured rat hippocampal neurons. Treatment of the neurons with KCl increased the density of the dendritic eEF1A mRNA clusters more than two-fold. FISH combined with IC revealed that the KCl treatment increased the density of eEF1A mRNA clusters that overlapped with or were immediately adjacent to PSD-95 clusters. These results indicate that KCl treatment increases both the density of eEF1A mRNA clusters and their synaptic association in dendrites of cultured neurons.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.22 no.4
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• pp.349-354
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• 2009

To rationally secure and maintain the safety and serviceability of a high-rise building, monitoring of structural responses of members is necessary. As such health monitoring of large-scale building structures has received growing attention by researchers in recent years. However, due to a very large number of members complexity of structural responses of a high-rise building structure, practical difficulties exist in selection of structural members to be sensored for assessment of structural safety of a structure. In this paper, a selection technique for active members for safety monitoring of a high-rise building based on displacement participation factor and strain energy density of a member is investigated.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.629-634
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• 2016

In this study, we investigated the effect of factors that influence the measurement of smoke density using synthetic rubber flooring. The characteristics of rolling stock in an enclosed environment can cause enormous loss of life by smoke inhalation during fires inside passenger cars. The amount of smoke generation from interior materials for rolling stock is strictly restricted domestically and in other countries. Precise measurement of smoke density is therefore required to assess the fire performance of interior materials. Major factors that influence the measurement of smoke density include the uniformity of the specimen, the variations in conditions and instruments, and the operational and maintenance environment of the instruments. The contribution of factors was analyzed by estimating the uncertainty to investigate the contribution ratios of the major factors. The results show a contribution ratio of about 86% for the variation from the measurement of light transmission using a photomultiplier tube. Thus, this factor was the most representative for the measurement of smoke density. The contribution ratio of the other factors was low at about 11%, including irradiant flux conditions (${\pm}0.5 kW/m^2$) and the influence of the operational and maintenance environment of the instrument. These results were obtained using specimens with high uniformity.

• Design & Manufacturing
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• v.13 no.2
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• pp.37-43
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• 2019

This study conducted a research as to condensation heat transfer friction loss headby using three types of flat micro multi-channel tubes with different processing of micro-fin and number of channels inside the pipes and different sizes of appearances. In addition, identical studies were conducted by using smoothing circular tubes with 5mm external diameter to study heat enhancement factor and pressure drop penalty factor. 1) The friction head loss showed an increase as the vapor quality and mass flux increased. In case of saturation temperature, it shows an increase as it gets lower. These factors are the reason occurring as the lower the saturation temperature is, the higher the density of refrigerant vapor gets. The influence of heat flux is similar as the dryness is low, but as it gets higher, it lowers in heat flux, and as the high temperature of high heat flux, it is a factor that occurs as the density gets lower. 2) RMS error of the in case of friction head loss, it showed to be predicted as 0.45~0.67 by Chisholm, Friedel, Lockhart and Martinelli. 3) As forfriction head loss penalty factor, the smaller the aspect ratio is, the larger the penalty factor gets, and as for the effect of micro-fin, the penalty factor increased because it decreases to the gas fluid the way groove for the refrigerant's flow.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.14 no.5
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• pp.370-376
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• 2004

The overall aim of this paper is to determine coupling loss factor of welding point between shell and cylinder using loss factor and structural loss factor. For this purpose, two kinds of loss factor were adopted. One is loss factor of each sub structure, another is structural loss factor based on the complex welded or assembled structure. Using these two parameters, it ispossible to derive the coupling loss factor which represent characteristic condition of SEA theory. Coupling loss factor of conjunction in complex structure was expressed as power balance equation. The derived equation for a coupling loss factor has been simplified on the assumption of one way (uni-directional) power flow between multi-sub structures. Using these conditions, it is possible to find the equation of coupling loss factor expressed as above two loss factors. To check the effectiveness of above equation, this paper used two-stage application. The first approach was application between simple cylinder and shell. The next was adopted rotary compressor. Rotary compressor has three main conjunctions between shell and internal vibration part. This equation was applied to find out the optimum welding point with respect to reduce the noise propagation. It shows the effective tool to evaluate the coupling loss factor in complex structure

• Communications for Statistical Applications and Methods
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• v.19 no.3
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• pp.495-500
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• 2012

We consider a Bayesian test of independence in a two-way contingency table that has some zero cells. To do this, we take a three-stage hierarchical Bayesian model under each hypothesis. For prior, we use Dirichlet density to model the marginal cell and each cell probabilities. Our method does not require complicated computation such as a Metropolis-Hastings algorithm to draw samples from each posterior density of parameters. We draw samples using a Gibbs sampler with a grid method. For complicated posterior formulas, we apply the Monte-Carlo integration and the sampling important resampling algorithm. We compare the values of the Bayes factor with the results of a chi-square test and the likelihood ratio test.

• Journal of the Korean Chemical Society
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• v.56 no.1
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• pp.20-27
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• 2012

Complex permittivity spectra of Allyl Chloride (AC), 2-Butanone (2-BU) and their binary mixtures over the entire range of concentration were obtained using the Time Domain Reflectometry (TDR) technique in microwave frequency range at various temperatures. Static dielectric constant and relaxation time are obtained from complex permittivity spectra. Density ($\rho$) and refractive index ($n_D$) are also measured. These parameters are used to determine excess dielectric constant, excess inverse relaxation time, excess molar volume, excess molar refraction, polarity, Bruggeman factor and thermodynamic parameters viz. enthalpy of activation and entropy of activation. The values of static dielectric constant and relaxation time increases while density and refractive index decreases with the percentage of 2-Butanone in Allyl Chloride increases. Excess parameters were fitted to a Redlich-Kister equation.

• Water for future
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• v.25 no.3
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• pp.79-86
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• 1992

ARF(Areal Reduction Factor) have been developed and used to convert point I-D-F to areal I-D-F in many countries. In Korea, through ARF was calculated in Han river basin by several researchers, it has limit to apply to other regions \ulcorner 새 low density of rainfall gauge station and shortage of data. In this study ARF has developed in areas of high density of rainfall gauge station, Pyungchang river(han river), Wi stream(nakdong river), and Bochung stream(Guem river) basin by fixed-area method. And coefficient of variation of annual mean precipitation was presented to use ARF in othere areas and its applicability was analyzed.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.10a
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• pp.147-152
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• 1999

Distribution of fracture system is an important factor to analyse instability of jointed rock slope. In the most case of rock slopes, joint distribution properties are related to potential, shape, size and locality of slope failure. The purpose of this paper is to present an application of fracture characterization related to rock slope failure. Fracture data used in this study are collected by scanline survey. Two aspects of fracture characterization for rock slope are handled in this study First, In order to determine the potential and shape of slope failure, trace length of joints is considered as the weighting factor about collected orientation data. Second, Relationship between trace length and spacing is analysed to estimate failure location and size. The distribution of fracture system is directly influenced on wedge failure. It is effective to analyse the orientation of fractures by using weighting factors associated with the trace length of fractures rather than to analyse only that of fractures. It gives a conclusion that the wedge failure occurred along the peak of fracture density(or intensity) cycles.