• 한국동물학회지
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• 제12권4호
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• pp.103-108
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• 1969

容接이 103,341$cm^3$의 鐵製網室에 Platanus sp.의 切樹를 植栽하고 1區를 3組씩으로 하여 H. cunea 의 幼蟲과 天敵의 比를 200:10, 400:10, 800:10 그리고 天敵을 投入하지 않은 對照區를 두어 H. cunea 의 P. maculiventris에 依한 被殺率을 化期別로 調査하였다. 1. H. cunea의 一化期에서 200:10 區는 94.6%, 400:10 區는 91.4%, 800:10區는 62.4%의 H. cunea의 被殺率을 보여주었다. 2. H. cunea의 二化期에서 200:10區는 96.3%, 400:10 區는 93.6%, 800:10區는 67%의 H. cunea의 被殺率을 보여주었다. 3. 對照區의 境遇는 H. cunea의 一化期에서 98.7%, 二化期에서 94.4%의 H. cunea가 幼蟲世代를 마쳤다.

• Journal of Gastric Cancer
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• 제20권2호
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• pp.190-201
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• 2020

Purpose: This study sought to investigate the prognostic significance of tumor-infiltrating lymphocytes (TILs) in relation to tumor location within the stomach. Materials and Methods: The densities and prognostic significance of TIL subsets were evaluated in 542 gastric cancer patients who underwent gastrectomy. Immunohistochemical staining for CD3, CD4, CD8, forkhead/winged helix transcription factor (Foxp3), and granzyme B was performed. Results: Cardia cancer was associated with significantly lower densities of CD8 T-cells and higher densities of Foxp3 and granzyme B T-cells than non-cardia tumors. Multivariate analysis showed that advanced age (hazard ratio [HR], 1.023; 95% confidence interval [CI], 1.006-1.040), advanced T classification (HR, 2.029; 95% CI, 1.106-3.721), lymph node metastasis (HR, 3.319; 95% CI, 1.947-5.658), low CD3 expression (HR, 0.997; 95% CI, 0.994-0.999), and a high Foxp3/CD4 ratio (HR, 1.007; 95% CI, 1.001-1.012) were independent predictors of poor overall survival in cardia cancer patients. In non-cardia cancer patients, total gastrectomy (HR, 2.147; 95% CI, 1.507-3.059), advanced T classification (HR, 2.158; 95% CI, 1.425-3.266), lymph node metastasis (HR, 1.854; 95% CI, 1.250-2.750), and a low Foxp3/CD4 ratio (HR, 0.978; 95% CI, 0.959-0.997) were poor prognostic factors for survival. Conclusions: The densities and prognostic effects of TILs differed in relation to the location of tumors within the stomach. The contrasting prognostic effects of Foxp3/CD4 ratio in cardia and non-cardia gastric cancer patients suggests that clinicians ought to consider tumor location when determining treatment strategies.

• 한국작물학회지
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• 제30권2호
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• pp.117-125
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• 1985

수도이앙의 생력화를 위한 소식을 함으로써, 수도의 종내경합에 의한 수량감소를 막고, 시비로 증수가능성이 큰 초형을 탐색하기 위하여 본 연구는 수행되었다. 공시한 3개 초형의 동질유전자 계통은 Open-type, Spread-type 및 Broom-type 이었으며, 이들을 $m^2$당 47.62, 22.22, 15.15, 11.11, 8.33주로 이앙하고, 시비는 N-P$_2$O$_{5}$-K$_2$O=15.0-7.0-8.0kg/10a를 표준으로 한 0, 0.5, 1.0, 1.5 배비로 하였다. 시험결과, 1. 소식에 따른 출수지연은 종내경합정도에 비례하여서 다비할수록 약화되었으며, 작물초형별로는 Broom$\longrightarrow$ Spread$\longrightarrow$ Open-type의 순이었다. 2. 잡초와의 종간경합은 소식.다비로 증대되었으며, 작물초형별로는 Broom-type에서 특히 다연생잡초종에 의한 경합이 커지는 경향이었다. 3. 시비량증대에 따라 천립중을 제외한 면적당수수.수당영화수.등숙률은 3초형 모두 증대경향을 나타냈으나 재식밀도에 따른 반응은 면적당수수만 정의 상관을 나타냈다. 4. 수량성은 Open-type과 Spread-type의 경우, 이앙밀도와 정상관을 보인 반면에 Broom-type에서는 수량차이가 인정되지 않았다. 5. $m^2$당 이앙밀도를 15, 11, 8주로 낮추었을 때, Open-type은 각각 1.12, 1.21 및 1.28배비로써 그리고 Broom-type은 각각 1.22, 1.35, 1.4배비로써 Open-type 표준수량의 보상이 가능하였다.다.

• Journal of Acupuncture Research
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• 제21권2호
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• pp.205-222
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• 2004

Objective: To investigate the effects of acupointed Choksamni(ST36), Kokchi(LI11) and Arbitrary acupoint on NADPH-diaphorase, neuronal nitric oxide synthase(nNOS), neuropeptide Y(NPY) and vasoactive intestinal peptide(VIP) in the cerebral cortex of spontaneously hypertensive rats. Methods: The experimental groups were divided into four groups: Normal, Choksamni(ST36), Kokchi(LI11), arbitrary group. Needles were inserted into acupoints at the depth of 0.5 cm with basic insertion method. Such stimulation was applied continuously for 10 minutes, every other day, for 10 sessions of treatments. Thereafter we evaluated changes in NADPH-d-positive neurons histochemically and changes in nNOS, NPY and VIP-positive neurons immunohistochemically. Results : The optical densities of NADPH-d-positive neurons of all the Choksamni & Kokchi groups were significantly different in all areas of cerebral cortex as compared to arbitrary group. In motor1, sensory2, cingulate2, insular, peripheral, visual cortex there was a significant difference between Choksamni & Kokchi group. The optical densities of nNOS-positive neurons of Choksamni group were significantly different in all areas except for auditory, visual and pisiform cortex and Kokchi group in all areas except for auditory and pisiform cortex as compared to arbitrary group. And there was a significant difference in cingulate1, cingulate2, ectohinal, visual cortex between Choksamni & Kokchi group. The optical densities of NPY neurons of Choksamni group were significantly different in cingulate2, insular, pisiform cortex and Kokchi group in motor1, motor2, sensory1, cingulate2, ectorhinal cortex as compared to arbitrary group. And there was no significant difference between Choksamni & Kokchi group. The optical densities of VIP neurons of Choksamni group were significantly different in all areas except for motor1, auditory cortex and Kokchi group in sensory1, insular, ectorhinal, perirhinal, visual, pisiform cortex as compared to arbitrary group. And there was a significant difference in cingulate1, cingulate2, retrosplenial, auditory corterx between Choksamni & Kokchi group. Conclusion : Our results demonstrated that acupuncture on Choksamni(ST36) & Kokchi(LI11) changes the control activities of the NO system in the cerebral cortex of SHR and according to areas there were significant difference between two groups. In all cerebral cortex areas there were distributed NPY & VIP and there were no significant difference among Choksamni(ST36), Kokchi(LI11) and arbitrary group.

• Journal of Oral Medicine and Pain
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• 제20권2호
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• pp.429-447
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• 1995

This study was designed to determine the most effective concentration of fluoride and levels of laser irradiation for the remineralization of decayed teeth. After irradiation with a pulsed Nd:YAG laser and the topical application of fluoride, phosphate and fluoride concentration in enamel were measured. And then the changes on surface enamel using an scanning electron microscope were observed. Samples by extraction healthy, permanent, mandibular bovine teeth with no caries were obtained. Among them 371 healthy samples were selected and artificial carious lesions were made. 20 samples were assigned to each experimental group. After irradiation with a pulsed Nd:YAG laser with total energy densities of 10J/$\textrm{cm}^2$, 20J/$\textrm{cm}^2$ for each group. On the teeth, 2% NaF, 1.9% NH4F, 1.6% TiF4 Elmex gel(amine fluoride) and 1.23% APF gel were applied. After pH circulatory procedures, concentrations of fluoride with and Ionalyzer (Orion Research, Model 901, USA) and phosphates with an Uv/V is spectrophotometer (Uvikon 860, Kontrom Co, Switzerland) were measured. By etching the teeth in layers and measuring fluoride concentrations, a profile of fluoride penetration according to depth could be developed. And also the changes on the surface of the enamel using an electron scanning microscope were observed. The comparative analysis yielded the following results : 1. Phosphate concentration was low in all groups compared with the control group except for teeth treated Elmex gel, irradiated with 10J/$\textrm{cm}^2$ and 30J/$\textrm{cm}^2$ energy densities. Teeth treated with APF gel and 30J/$\textrm{cm}^2$ irradiation gad the lowest phosphate concentration. 2. Among all groups, fluoride concentrations in tooth enamel were highest in APF gel and NaF groups irradiated at 30J/$\textrm{cm}^2$. The APF gel group had the highest fluoride concentrations across all energy densities. 3. In the APF gel group, and the NaF group, the greater the energy density of the laser, the higher the fluoride concentrations in the enamel. 4. In all groups, the concentration of fluoride in the enamel by depth tended to decrease with depth. 5. Under the scanning electron microscope, under the condition of irradiation with 20J/$\textrm{cm}^2$, enamel crack was detected for the first time. In the NH4F group, spherical deposits were found on the surface of the enamel, and in the TiF4 group the surface of enamel was covered with an irregular, thin membranous mass in places. In the APF gel and NaF groups irradiated with 10J/$\textrm{cm}^2$, spherical and irregular particles covered the teeth. When these groups were irradiated at 20J/cm2, they were covered with amorphous crystals. These results suggest that one could obtain more effective anticariogenic effects without damage to teeth when less than 20J/$\textrm{cm}^2$ energy densities and APF gel are used.

• Elastomers and Composites
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• 제48권4호
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• pp.288-293
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• 2013

무수말레산-그래프트 EPDM (MAH-g-EPDM)/산화아연 복합체를 디클로로아세트산(DCA)/톨루엔 공용매로 처리하고 추출 온도에 따른 무게 감소와 가교밀도 측정을 이용하여 가교 특성을 조사하였다. 감쇠전반-후리에변환 적외선분광법(ATR-FTIR)을 이용하여 화학적 변화를 분석하였다. 상온 추출보다 고온($90^{\circ}C$) 추출에 의한 무게 감소가 월등히 높았으며, DCA/톨루엔 공용매 추출에 의한 무게 감소는 톨루엔 추출에 의한 무게 감소보다 5배 이상 높았다. 용매 추출 후 가교밀도를 측정하였으며, 1차 가교밀도보다 2차 가교밀도가 높았다. 1차 가교밀도는 추출온도가 높은 경우 더 낮았고 DCA/톨루엔 공용매로 추출한 것이 톨루엔으로 추출한 것보다 훨씬 낮았다. 2차 가교밀도는 DCA/톨루엔 공용매로 추출한 것이 톨루엔으로 추출한 것보다 높았다. 고온에서 DCA/톨루엔 공용매로 추출하면 강한 가교 그물망만 남는 반면, 상온에서 톨루엔으로 추출하면 미가교 고분자 사슬이 추출되는 등 추출 용매와 온도에 따라 추출되는 성분이 달랐다. 따라서 추출 용매와 온도에 따른 추출 성분의 비교와 연속 가교밀도 측정에 의해 MAH-g-EPDM/산화아연 복합체의 가교 특성을 분석할 수 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.133-133
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• 2017

Lodging is one of the important constraints in rice production. The lodging destroys the canopy structure, and sharply reduces the capacity of photosynthetic rate and dry matter production. In cereal crops, stem lodging can be classified into two types: stem breaking type and stem bending type. To improve stem lodging resistance, it is important to reveal strong culm traits of superior lodging resistant varieties. There are large varietal differences in parameters associated with the bending moment at breaking (M) and flexural rigidity (FR). The indica variety Takanari possesses large M due to its large section modulus (SM) despite of its small bending stress (BS), while Takanari also has large FR due to its large secondary moment of inertia (SMI) and Young's modulus (YM). To identify quantitative trait loci (QTLs) and the corresponding genes associated with the parameters for M ($=SM{\times}BS$) and FR ($=SM{\times}YM$) should enable to develop lodging resistant varieties, efficiently. In order to identify QTLs for cell wall materials such as cellulose, hemicellulose and lignin associated with BS and YM, a set of Chromosome Segment of Substitution Lines (CSSLs) consisted of 37 lines with chromosome segments of Koshihikari in the genetic background of Takanari were used. Takanari had large M and small BS as compared with Koshihikari. The QTLs for BS were estimated on chromosomes 3, 5, 6, 8, 9, 10, 11 and 12. Koshihikari alleles increased BS in these QTLs. Takanari had a large FR due to its large SMI and YM as compared with Koshihikari. The YM was increased by substitution of the Koshihikari chromosomal segments on chromosomes 2, 10 and 11. Other QTLs estimated on chromosomes 7 and 12 that Koshihikari alleles contributed to the decrease of YM. For lignin, only one major QTL for lignin density was detected on chromosome 11. Hollocellulose densities were increased by the substitution of Koshihikari segments on chromosomes 5 and 11. On the other hand, these were decreased on chromosomes 1 and 3 by substitution of Koshihikari segments. QTLs for cellulose density were estimated on chromosomes 1, 3 and 5 by substitution of Koshihikari segments. For hemicellulose, QTL on chromosome 3 showed that hemicellulose density decreased by the substitution of Koshihikari segment. However, hemicellulose densities on chromosomes 5, 8 and 11 showed the opposite effects. The QTLs for hemicellulose, cellulose, and hollocelulose densities identified on chromosome 5 overlapped with that for bending stress, indicating the positive effect of Koshihikari segment on increasing bending stress. These results suggest that some QTLs for the densities of cell wall materials contribute to increasing bending stress and Young's modulus, and could be utilized to improve the lodging resistance for both types of breaking and bending in rice varieties.

• Applied Microscopy
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• 제22권1호
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• pp.42-54
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• 1992

To understand the structural changes of the myocardial myocytes and endothelial cells in ischemic and reperfused heart, and to elucidate their roles in those conditions, the authors observed cat and rat myocardium ultrastructurally and evaluated them with morphometric techniques. In cat, mild ischemia and moderate degree reperfusion injury was induced by ligation of the anterior interventricular branch of left coronary artery and reperfusion. In rat, severe ischemia and irreversible reperfusion iniury was made using in vitro Langendorff techniques. In normal cat myocytes, the volume densities of cytoplasm, myofibrils, mitochondria, sarcoplasmic reticulum and T tubules were $0.11{\pm}0.013,\;0.51{\pm}0.096,\;0.25{\pm}0.082,\;0.09{\pm}0.008,\;0.02{\pm}0.010$ (Mean${\pm}$S.D.) respectively, and the myofibril/mitochondria ratio was $2.33{\pm}1.379$. The numerical density and average volume of mitochondria were $0.76{\pm}0.210/{\mu}m^3$ and $0.33{\pm}0.057{\mu}m^3$ respectively. In normal cat endothelial cells, the volume densities of cytoplasm, cytoplasmic vesicles, tubular systems (including endoplasmic reticulum and Golgi apparatus) and mitochondria were $0.43{\pm}0.023,\;0.28{\pm}0.007,\;0.22{\pm}0.021,\;0.03{\pm}0.014$ respectively. The mean thickness of endothelial cells was $230{\pm}45.2{\mu}m$. The numerical density and average volume of cytoplasmic vesicles were $508{\pm}55.0/{\mu}m^3,\;578{\pm}104.8nm^3$ respectively. In cat myocytes which received mild ischemic injury, the volume densities of organelles were not changed significantly in ischemic and reperfusion states. In reperfusion group myocytes, the numerical density of mitochondria was decreased significantly and the average volume was increased significantly. In endothelial cells, the volume density of tubular system in ischemic group and the average volume of cytoplasmic vesicles in reperfusion group were increased significantly. In rat myocytes which received severe ischemic injury, the volume density and average volume of mitochondria were increased significantly, and the volume density of sarcoplasmic reticulum and numerical density of mitochondria were decreased significantly in both ischemic and reperfusion groups. In ischemic and reperfused endothelial cells, the volume density and numerical density of cytoplasmic vesicles, the volume density of cytoplasm were decreased significantly. The volume densities of tubular system were increased significantly in both ischemic and reperfused groups. The volume density of mitochondria in ischemic group and the average volume of cytoplasmic vesicles in reperfusion group showed significant increase. The authors, based on the above observations, conclude that the mitochondria of myocytes and the cytoplasmic vesicles of endothelia are the first group of targets in ischemic and reperfusion injury and in this respect, the degree of ischemic insult is not significant. The role of myocyte mitochondria in reperfusion injury may be insignificant, but endothelial cells may contribute actively to reperfusion injury.

• 농약과학회지
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• 제18권3호
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• pp.175-180
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• 2014

비트에서 흰띠명나방의 경제적 피해수준과 경제적 피해허용수준을 설정하하기 위한 연구를 수행하였다. 2010년도 8월 하순에 비트를 정식하였으며 흰띠명나방 2령 애벌레 밀도를 달리하여 접종한 후 생육시기별로 피해엽률을 조사하였다. 10주를 한 반복으로하여 각 처리구당 3반복으로 실험하였다. 비트에서 흰띠명나방의 피해엽률은 접종마리수가 증가함에 따라 피해엽률도 증가하였으나 10월 초순경에 감소하다가 하순경에 다시 증가하였다. 흰띠명나방 애벌레밀도는 초기 접종밀도가 높을수록 증가하였고, 9월 중순부터 감소하다가 10월 중순에 다시 증가하였다. 흰띠명나방 접종밀도가 증가할수록 비트 잎의 수확량과 상품률이 감소하였으며 이에 따라 수량감소율은 증가하였다. 흰띠명나방 유충을 접종한 후 3회에 걸쳐 잎을 수확하여 얻은 접종밀도(x)와 수량감소율(y1)과의 관계식은 y1 = 1.226x + 3.36이였으며 최종적으로 수학한 뿌리의 수량감소율(y2)과의 관계식은 y2 = 0.478x + 1.15이었다. 따라서 잎의 경제적 피해수준은 10주당 1.3마리, 경제적 피해허용은 1.1마리였으며, 뿌리의 경제적 피해수준은 10주당 8.1마리, 경제적 피해허용수준은 6.4마리로 설정되었다.

• 한국산림과학회지
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• 제109권3호
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• pp.281-290
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• 2020

본 연구는 식재밀도가 느티나무 조림목의 초기 생장에 미치는 영향을 구명하고자 수행되었다. 연구지는 경북 경산과 전북 순창이며, 2015년 3월 말 느티나무 노지묘(1-0)를 4처리 밀도(3천, 5천, 7천, 10천본 ha^-1)로 식재하였다. 2019년까지 매년 9월에 조림목의 근원경과 수고를 측정하였고, H/D율과 수간 재적을 계산하였다. 모든 조사지에서 식재밀도가 증가할수록 근원경 및 수고 생장이 높아지는 경향을 보였으며, 수간 재적은 10천본 ha^-1 처리구에서 가장 높게 나타났다. 그러나 생존율과 H/D율은 식재밀도에 따른 차이가 없었다. 식재후 5년차에, 경산 지역에서의 근원경, 수고, 수간 재적은 순창 지역에서보다 높게 나타났다. 그러나, 입지 환경이 다른 두 조사지 모두에서 식재밀도 증가에 따라 조림목의 초기 생장이 향상되는 동일한 경향을 확인할 수 있었다. 본 연구는 10천본 ha^-1의 식재밀도가 느티나무 조림목의 초기 생장을 향상시키기 위한 적정 식재밀도임 확인하였으며, 느티나무의 조림학적 특성으로서 밀식처리에 의해 초기 생장이 향상될 수 있다는 점을 제안한다.