• Korean Journal of Soil Science and Fertilizer
• /
• v.28 no.2
• /
• pp.135-144
• /
• 1995

Understanding on nutrient solute movement during the course of freezing and thawing was attempted through laboratory and field obsevations. Small sectioned tubes with 5cm inner diameter, 0.2cm thick and 1cm long were connected to 30cm long soil columns for laboratory study. The columns were filled with soil, and treated with 20mmol/kg $KNO_3$ for upper 5cm. The upper end was set in the freezing section, and the lower end was set in the refrigerating section of a refrigerator. Temperature was controlled at $-7({\pm}1)^{\circ}C$ and $1.5({\pm}1)^{\circ}C$, respectively. After top 5cm soil was frozen, the columns were sectioned, and analyzed for $NO_3^-$, $NH_4^+$ and $K^+$. For field study, the 20cm inner diameter and lm long soil columns were installed in Chuncheon and Daegwanryung, where the altitude was 74m and 840m, respectively. The soils used were silt loam and clay loam. The top 20cm soils were treated with 50mmol/kg as $KNO_3$. The soil columns were taken during winter freezing and after thawing. By laboratiry study, upward movement of $NO_3^-$ and $K^+$ during the course of freezing was confirmed. The upward movement of $K^+$ was, however, one fifth to one tenth of $NO_3^-$. The upward movement of inorganic nitrogen as well as laboratory during the course of freezing, but large amount of nitrogen was lost from the profile after thawing in early spring. Leached nitrogen from the upper 20cm to lower part was 17 to 24 percents. The maximum depth of leaching during the experiment was 50cm for all soils. The net loss of inorganic nitrogen from the whole profile ranged 8.7 to 39.5 percents. The net loss was greater in Daegwanryung where temperature was lower and snowfall was larger than Chuncheon, and the loss was greater from the silt loam soil than clay loam soil of which percolation rate was small. The results implied that reasons for nitrogen loss during the winter might include surface washing by snow melt as well as leaching and denitrification.

• Korean Journal of Environmental Agriculture
• /
• v.37 no.4
• /
• pp.235-242
• /
• 2018

BACKGROUND: About 81% of nitrous oxide ($N_2O$) emissions from agricultural land to the atmosphere is due to nitrogen (N) fertilizer application. Mitigation of $N_2O$ emissions can be more effective in controlling biochemical processes such as nitrification and denitrification in the soil rather than decreasing fertilizer application. The use of urease inhibitors is an effective way to improve N fertilizer efficiency and reduce $N_2O$ emissions. Several compounds act as urease inhibitors, but N-(n-butyl) thiophosphoric triamide (NBPT) has been used worldwide. METHODS AND RESULTS: Hot pepper and chinese cabbage were cultivated in five treatments: standard fertilizer of nitrogen-phosphorus-potassium(N-P-K, $N-P_2O_5-K_2O$: 22.5-11.2-14.9 kg/ha for hot pepper and $N-P_2O_5-K_2O$: 32.0-7.8-19.8 kg/ha for chinese cabbage), no fertilizer, and NBPT-treated fertilizer of 0.5, 1.0, and 2.0 times of nitrogen basal application rate of the standard fertilizer, respectively in Gyeonggi-do Hwaseong-si for 2 years(2015-2016). According to application of NBPT-treated fertilizer in hot pepper and chinese cabbage, $N_2O$ emission decreased by 19-20% compared to that of the standard fertilizer plot. CONCLUSION: NBPT-treated fertilizer proved that $N_2O$ emissions decreased statistically significant in the same growth conditions as the standard fertilization in the hot pepper and chinese cabbage cultivated fields. It means that NBPT-treated fertilizer can be applied for N fertilizer efficiency and $N_2O$ emissions reduction.

• Korean Journal of Microbiology
• /
• v.54 no.4
• /
• pp.369-378
• /
• 2018

This study was conducted to evaluate effects of dietary microbial probiotics on the growth and disease resistance of olive flounder (Paralichthys olivaceus) in a recirculating aquaculture system (RAS), and the effects of the probiotic bioaugmentation on the microbial community structure and water quality. For the analysis, 80 juvenile fish (average weight, $25.7{\pm}7.6g$; average length, $15.2{\pm}1.7cm$) were fed a basal diet containing a commercial microbial product CES-AQ1 (CES; $1{\times}10^9\;CFU/kg$ diet) in an RAS for 8 weeks. Weight gain, the specific growth rate, feed efficiency, and protein efficiency ratio of the fish fed the CES diet in the RAS were 1.5~2.5 times higher than those of fish fed the basal diet alone, or the basal diet containing oxytetracycline (OTC), yeast plus bacterium, or Bacillus subtilis in a still water system. There was no significant difference in the pathogen challenge test between fish fed the OTC diet and fish fed the CES diet in the RAS, suggesting the CES-AQ1 probiotic used in the RAS as a potential replacement for antibiotics. The RAS biofilter maintained the highest microbial diversity and appeared to harbor microbial communities with ammonium oxidation, denitrification, and fish pathogen suppression functions. Ammonia, which is hazardous to fish, was significantly decreased to < 0.5 mg/L in 19 days, indicating the effectiveness of probiotic supplementation to maintain good water quality in RAS. These results suggest that the intestinal microbial communities of fish are stabilized by a probiotic-containing diet (CES) and that bioaugmentation with probiotics may be an eco-friendly and economical supplement for aquaculture of olive flounder, promoting both good water quality and fish health in an RAS.