• BMB Reports
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• v.28 no.4
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• pp.336-341
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• 1995

Using a mutant M13 phage derivative lacking a great part of the complementary strand synthesis origin, we identified six single-strand initiation (ssi) signals for DNA replication in pACYC184, pLG214, pGKV21, and pDPT270 plasmids, and named them $ssiA_{YC}$, $ssiA_{LG}$, $ssiB_{LG}$, $ssiA_{KV}$, $ssiA_{PT}$, and $ssiB_{PT}$, respectively. Two of them were from pDPT270, one from downstream the on of pACYC184, two from pLG214, one from upstream the plus origin of pGKV21. Introduction of these ssi signals into the deleted $ori_c$ site of a mutant filamentous M13 phage ($M13{\Delta}lac182$) resulted in the restoration of growth activity of this phage. These ssi signals were classified into a number of groups on the basis of sequence similarity. $ssiA_{YC}$ and $ssiA_{LG}$ show extensive sequence homology to the n'-site (primosome assembly sites) of ColE1, whereas $ssiB_{PT}$ is homologous to the n'-site of ${\Phi}X174$. $ssiA_{PT}$ belongs to G4-type ssi signals which require only dnaG primase and SSB protein for the priming of replication. In addition, possible biological roles of these ssi signals are discussed.

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.206-211
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• 2004

Levansucrase gene(lscA) from Pseudomonas aurantiaca was subcloned downstream of GAL1 promoter in pYES 2.0 and pYInu-AT [GAL10 promoter ＋ exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-lscA and p YInu-lscA, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Sacchromayces cerevisiae SEY2102, and transformants with high activity of levansucrase were selected. When each yeast transform ants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 8.62 U/ml with the strain harboring pYES-lscA and 5.43 U/ml with the strain harboring pYInu-lscA. The levansucrase activity of 80% was detected in the periplasmic space and cytoplasm. The levansucrase activity in the medium of SEY2102/pYInu-lscA was 0.87 U/ml whereas that of SEY2102/pYES-lscA was 0.47 U/ml, which implying the exoinulinase signal sequence didn't enhance the secretion efficiency of levansucrase. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1868-1873
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• 2006

A novel microorganism that could degrade high molecular weight gellan was screened and isolated from soil. On gellan plate, the microorganism grew well and completely liquefied the plate. The gellan-degrading microorganism was isolated by pure culture on glucose and nutrient agar medium afterwards. The 16S rDNA sequence analysis and biochemical tests using an API 50CHB/20E kit revealed that the strain belonged to Bacillus sp. The isolate, named as Bacillus sp. YJ-1, showed optimum gellan-degrading activity in 0.5% gellan medium at pH 7.5 and 37$^{\circ}C$. The activity was measured and evaluated by the thiobarbituric acid and thin-layer chromatography method. Mass spectrometry revealed that the major gellan.. depolymerized product was an unsaturated tetrasaccharide consisting of $\Delta$4,5-glucuronic acid-(1$\rightarrow$4 )-$\beta$-D-glucose-(1$\rightarrow$4)- $\alpha$-L-rhamnose-(1$\rightarrow$3)-$\beta$-D-glucose, which is a dehydrated repeating unit of gellan, thus the enzyme was identified as gellan lyase. When the gellan was present in the medium, the gellan-degrading activity was much higher than that in glucose-grown cells. These results indicate that in the presence of gellan, Bacillus sp. YJ-1 is able to metabolize the gellan by inducing gellan-degrading enzymes that can degrade gellan into small molecular weight oligosaccharides, and then the gellan. depolymerized products are taken up by the cells and utilized by intracellular enzymes.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.1005-1012
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• 2020

Acid mine drainage (AMD) has been a serious environmental issue that threatens soil and aquatic ecosystems. In this study, an acid-tolerant sulfate-reducing bacterium, strain S4, was isolated from the mud of an AMD storage pond in Vietnam via enrichment in anoxic mineral medium at pH 5. Comparative analyses of sequences of the 16S rRNA gene and dsrB gene involved in sulfate reduction revealed that the isolate belonged to the genus Desulfovibrio, and is most closely related to Desulfovibrio oxamicus (with 99% homology in 16S rDNA sequence and 98% homology in dsrB gene sequence). Denaturing gradient gel electrophoresis (DGGE) analyses of dsrB gene showed that strain S4 represented one of the two most abundant groups developed in the enrichment culture. Notably, strain S4 was capable of reducing sulfate in low pH environments (from 2 and above), and resistance to extremely high concentration of heavy metals (Fe 3,000 mg/l, Zn 100 mg/l, Cu 100 mg/l). In a batch incubation experiment in synthetic AMD with pH 3.5, strain S4 showed strong effects in facilitating growth of a neutrophilic, metal sensitive Desulfovibrio sp. strain SR4H, which was not capable of growing alone in such an environment. Thus, it is postulated that under extreme conditions such as an AMD environment, acid- and metal-tolerant sulfate-reducing bacteria (SRB)-like strain S4 would facilitate the growth of other widely distributed SRB by starting to reduce sulfate at low pH, thus increasing pH and lowering the metal concentration in the environment. Owing to such unique physiological characteristics, strain S4 shows great potential for application in sustainable remediation of AMD.

• Proceedings of the Korea Society of Information Technology Applications Conference
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• 2002.11a
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• pp.407-411
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• 2002

Ternary sequences are digital codes consisting of discrete values -1, 0, and 1 only. They are advantageous in that the correlation can be carried out using additions only. Also, they feature an ideal circular autocorrelation function, but in channel characterization tasks, the usual requirement is that the linear autocorrelation function be ideal, i.e., a Kronecker delta function. In this article, we consider two approaches to improving their linear autocorrelation or crosscorrelation properties: one is an inverse filtering method with thresholding, and the other is a singular value decomposition (SVD) method. Both methods are simulated under noisy circumstances. The inverse filtering method resulted in an improvement in peak sidelobe level of about 11 dB at an SNR of 30 dB, and the SVD method showed similar performances, albeit more sensitive to noise depending on the singular value selection strategy.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.8 no.2
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• pp.167-175
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• 1988

The principle objective of this study is to evaluate the fatigue behavior of structural steel components of highway bridges subjected to service stresses. The main aspects of this investigation are; 1) a measurement and statistical analysis of service stress cycles observed in highway bridge. 2) fatigue tests under equivalent constant-amplitude(CA) loading and simulated variable-amplitude(VA) loading 3) a evaluation of the fatigue behavior under VA-loading by eqivalent root mean cube (RMC) stress range. Theoretically, the RMC model is adequate in evaluation of fatigue behavior under VA-loading, because the regression coefficient (m) of crack growth rate is 3 approximately. The result of fatigue test shows that the RMC model is fitter than the current RMS model in fatigue evaluation under VA-loading. The interaction effects and sequence effects under VA-loading affect little fatigue life of structural components. As the transition rate of stress ranges is higher, the crack growth rate is higher.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2002.11a
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• pp.407-411
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• 2002

Ternary sequences are digital codes consisting of discrete values -1, 0, and 1 only. They are advantageous in that the correlation can be carried out using additions only Also, they feature an ideal circular autocorrelation function, but in channel characterization tasks, the usual requirement is that the linear autocorrelation function be ideal, i.e., a Kronecker delta function. In this article, we consider two approaches to improving their linear autocorrelation or crosscorrelation properties: one is an inverse filtering method with theresholding and the other is a singular value decomposition (SVD) method. Both methods are simulated under noisy circumstances. The inverse filtering method resulted in an improvement in peak sidelobe level of about 1㏈ at an SNR of 30㏈, and the SVD method showed similar performances, albeit more sensitive to noise depending on the singular value selection strategy.

• PNF and Movement
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• v.19 no.2
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• pp.233-242
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• 2021

Purpose: The purpose of this study was to determine the changes in foot contact area and pressure when walking with a functional insole that emphasizes the Hallux point as compared to a general insole. Methods: In this study, an experiment was conducted to investigate changes in plantar pressure and contact area for a functional insole that emphasized the Hallux point as compared to a general insole. A lower extremity robot was used for walking reproduction. First, the gait sequence according to the two insoles was determined through a randomized controlled trial comparison. According to the sequence procedure, the insole was attached to the shoe and then worn on the right side of the lower extremity robot for gait reproduction at a normal gait speed of 20 steps per minute. After programming the robot to walk, the experiment was carried out. The result value was determined by averaging the pressure and area data of the fore and rear foot measures after walking at 20 steps per minute. Results: The functional insole that emphasized the hallux point significantly increased the forefoot and rearfoot contact area (p < 0.05) and significantly decreased the forefoot and rearfoot contact pressure (p < 0.05) compared to the general insole. Conclusion: A functional insole that emphasizes the hallux point does not collapse the medial longitudinal arch during gait, increasing foot stability and reducing fatigue. Thus, this functional insole needs to be widely used clinically.

• The Korean Journal of Petroleum Geology
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• v.6 no.1_2 s.7
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• pp.20-24
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• 1998

Expert system is one type of artificial intelligence softwares that incorporate problem-solving knowledges and experiences of human experts by use of symbolic reasoning and rules about a specific topic. In this study, an expert system, PLAYMAKER2, is used to interpret sedimentary facies and depositional settings of the sedimentary sequence. The original version of the expert system, PLAYMAKER, was developed in University of South Carolina in 1990, and modified into the present PLAYMAKER2 with some changes in the knowledge-base of the previous system. The late Cenozoic sedimentary sequence with maximum 10,000 m in thickness, which is located in the Korean Oil Exploration Block VI-1 at the southwestern margin of the Ulleung Basin, is analysed by the expert system, PLAYMAKER2. The Cenozoic sedimentary sequence is divided into two units-lower Miocene and upper Pliocene-Pleistocene sediments. The depositional settings and sedimentary facies of the Miocene sediments interpreted by PLAYMAKER2 in terms of belief values are: for depositional settings, slope; $57.4\%$, shelf; $21.4\%$, basin; $10.1\%$, and for sedimentary facies, submarine fan; $35.7\%$, continental slope; $26.3\%$, delta; $16.1\%$, deep basinplain; $6.1\%$ continental shelf; $3.2\%$, shelf margin; $1.4\%$. The depositional settings and sedimentary facies of the Pliocene-Pleistocene sediments in terms of belief values we: for depositional settings, slope; $59.0\%$, shelf; $22.8\%$, basin; $7.0\%$, and for sedimentary facies, delta; $24.1\%$, continental slope; $22.2\%$, submarine fan; $17.3\%$, continental shelf; $7.0\%$, deep basinplain; $4.8\%$, shelf margin; $2.6\%$. The comparison of the depositional settings and sedimentary facies consulted by PLAYMAKER2 with those of the classical interpretation from previous studies shows resonable similarity for the both sedimentary units-the lower Miocene sediments and the upper Pliocene-Pleistocene sediments. It demonstrates that PLAYMAKER2 is an efficient tool to interpret the depositional setting and sedimentary facies for sediments. However, to be a more reliable system, many sedimentologists should work to refine and add geological rules in the knowledge-base of the expert system, PLAYMAKER2.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.70.2-70.2
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• 2013

We present collections of optical photometry for a pre-main sequence star HBC 722. It showed large amplitude optical outburst (${\Delta}V=4.7$ mag) in 2010 and classified as a FU Orionis type object. We have been observing HBC 722 from 2011 April to 2013 May, using Camera for QUasars in EArly uNiverse (CQUEAN) attached to the 2.1 m Otto Struve telescope at the McDonald Observatory. Time-series monitoring data (minute-scale interval) were obtained in SDSS r, i and z bands to see short-scale behaviors as well as trace the long-term brightness changes after the eruption in 2010. Interestingly, it started to brighten from 2011 early summer and became brighter than the first outburst peak in our 2013 May observation. We expect that the recovering phase would result from re-increase of disk accretion rate, might attribute to distinctive short-scale color features. In this presentation, we report long- and short-timescale optical behaviors of HBC 722 in the post-outburst phase.