• Journal of Applied Biological Chemistry
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• 제60권3호
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• pp.257-263
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• 2017

A cellulolytic strain Y2 was isolated from soil obtained in the Canadian Alpine region. The isolate was identified as Paenibacillus sp. Y2 by 16S rRNA sequencing. When grown in LB medium supplemented with carboxymethyl-cellulose (CMC), CMCase production increased to 122.0% of that observed in LB without CMC. Culture supernatant was concentrated by ultrafiltration and 80% ammonium sulfate precipitates were separated by Hi-Trap Q and CHT-II chromatography. The purified enzyme (EG-PY2) showed a homogeneous single band and the molecular mass was estimated to be 38 kDa by SDS-PAGE. Optimum pH and temperature of the enzyme were 4.5 and $30^{\circ}C$, respectively. The half-life of enzyme activity at 50 was 140.7 min, but the enzyme was drastically inactivated within 5 min at $55^{\circ}C$. The enzyme was highly activated to 135.7 and 126.7% by 5.0 mM of $Cu^{2+}$ or $Mg^{2+}$ ions, respectively, and moderately activated by $Ba^{2+}$ and $Ca^{2+}$ ions, whereas it was inhibited to 76.8% by $Fe^{2+}$, and to ${\leq}50%$ by $Mn^{2+}$, $Co^{2+}$, $Zn^{2+}$, and EDTA. The enzyme was activated to 211.5% in the presence of 0.5 M of NaCl and greatly tolerant to 3.15M of NaCl. The enzyme showed 2.98 times higher ${\beta}$-glucanase activity than CMCase activity. Based on these results, it can be concluded that EG-PY2 is an acidophilic, cold-active, and halotolerant endoglucanase. The authors suggest it is considered to be useful for various industrial applications, such as, fruit juice clarification, acidic deinking processes, high-salt food processing, textile and pulp industries, and for biofuel production from seaweeds.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 1999년도 Proceedings of Pre-symposium of the 10th ISWPC
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• pp.159-163
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• 1999

Model experiments were conducted to investigate the effect of different fillers on the removal of toner ink. Combinations of different papers (commercial photocopy paper and filler-free paper), fillers (calcium carbonate, kaolin clay, and talc), and chemicals(1-octadecanol, stearic acid, oleic acid, and TOFA) and stearic acid were found to be effective in detachment and agglomeration of toner ink. Furthermore, pH had little effect on toner detachment and agglomeration, indicating both protonated fatty acids and their anions are equally effective. In the presence of either kaolin clay or talc, all these agglomerating agents are equally effective, although a slightly higher dosage(1% for clay and 2% for talc as compared with control) is required, presumably due to the adsorption of chemical by the filler. Calcium carbonate filler, on the other hand, has a significant and adverse effect on the fatty acids used but has little effect on 1-octadecanol with the exception of possible adsorption. While stearic acid is not effective, a much higher level of oleic acid or TOFA is needed when calcium carbonate fillers are present as compared to the filler-free case. Fatty acids react with calcium carbonate to form calcium salts. The availability of fatty acid anion for toner detachment and agglomeration is determined by the solubility of calcium salt of a given fatty acid. Calcium oleate is 10 times more soluble in water than calcium stearate.

• 공업화학
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• 제10권2호
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• pp.299-303
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• 1999

경제적, 환경적인 이유로 신문용지를 생산하는데 신문고지가 상당량 재이용되고 있다. 본 연구에서는 국내 외 신문고지의 열화기간에 따른 제반 성질의 변화를 조사하기 위하여 시도하였다. 시료의 백색도, 수율 및 펄프 강도를 고려하면 열화기간이 증가할수록 탈묵성이 호주산 신문고지에서 가장 우수하고, 국내 신문원지를 사용한 신문고지와 일본 신문고지는 비슷하며, 카나다산 신문원지를 사용한 신문고지에서 가장 낮았다. 국내 신문원지를 사용한 신문고지의 경우, 열화기간 90일까지는 약 83%의 수율과 백색도 50% 이상을 나타내지만 그 이후에는 급격히 감소하였다. 또한 국내 신문용지의 경우 수입된 신문용지보다 섬유의 길이는 짧지만 제반 강도적성질은 높았다. 국내 신문용지와 수입 신문용지는 전반적으로 열화기간에 따라 인장강도와 파열강도는 증가하다가 감소하였으며, 인열강도는 점차 증가하였다.