• Journal of the Society of Naval Architects of Korea
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• v.54 no.6
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• pp.522-529
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• 2017

Air gun shock system is used as an alternative energy source as part of the attempt to overcome the restrictions of economical expense and environmental damage, etc., due to the use of explosives for the UNDerwater EXplosion (UNDEX) shock test. The objectivity of this study is to develop the simulation technique of air gun shock test for the design of model-scale one for the near field non-explosive UNDEX test through its verification with full-scale SERCEL shock test result. Underwater blasting response analysis of full-scale air gun shock test was carried out using highly advanced M&S (Modeling & Simulation) system of FSI (Fluid-Structure Interaction) analysis technique of LS-DYNA code, and was verified by comparing its shock characteristics and behaviors with the results of air gun shock test.

• Clinical and Experimental Reproductive Medicine
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• v.45 no.2
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• pp.82-87
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• 2018

Objective: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. Methods: Mice (6-8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size. A 1-mL blood sample was taken and the sera were separated. The oxidant-antioxidant balance was evaluated using a fast and reliable PAB assay. The results were expressed as $mean{\pm}standard$ deviation. Results: The mean PAB values (HK units) in the IVF-conceived and naturally conceived groups were $59.70{\pm}22.30$ and $54.70{\pm}18.22$, respectively (p= 0.82). Conclusion: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured.

• Fashion & Textile Research Journal
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• v.11 no.2
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• pp.286-292
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• 2009

The purpose of this study is to analyze the pattern of the present flight suit, to select variables of human body and key dimensions for making pattern of the flight suit, and to decide the most appropriate size of human body and rate of ease for designing an overall flight suit. For analyzing the pattern of flight suit, we used "M95Special" (men ; chest circumference 92-97 cm ; height : above 177 cm), which was indicated as the standard of the Ministry of National Defense. We analyzed general appearance and the actual dimensions of the pattern of the flight suit. As a result of analyzing the pattern, flight suit was front-opened overall style which had a flat collar with rounded edges, two-pieced sleeves with lower height of the sleeve's cap, 3cm-width belt on the waist and 8 pockets. To decide the most appropriate size of human body, we selected 20 variables of human body in the 359 variables of the Size Korea by referring the previous studies. The 20 variables of human body were 9 circumferences (chest, waist, hip, neck, armscye, wrist, thigh, knee, and ankle), 9 lengths (stature, back interscye fold, waist back, crotch height, crotch, body rise, arm, outside leg and total), and 2 breadths (biacromial and chest). We drew out the size of 20 variables from the Size Korea and US Army anthropometry data and decided the most appropriate values from the drawn data for applying to the pattern of flight suit.

• Journal of the Korea Institute of Military Science and Technology
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• v.17 no.5
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• pp.694-702
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• 2014

One of the most important devices in an underwater vessel is a propulsion system. It should be a quiet and efficient system for stealthy operations in the large mission area. Hence lead-acid battery system has been used to supply the energy to electric motor. Recent technological developments and improvements, such as polymer electrolyte membrane(PEM) fuel cell and lithium polymer battery and have created the potential to improve overall power and propulsion performance. An underwater vessel always starts their mission with a limited energy and is not easy to refuel. Therefore design of energy elements, such as fuel cell and battery, and their load distribution are important to increase the maximum operating time of underwater vessel. In this paper, the lead-acid battery/PEM fuel cell and lithium polymer battery/PEM fuel cell were suggested as propulsion system and their performances were analyzed by modeling and simulation using Matlab/Simulink. Each model concentrated on representing the characteristics of energy element depending on demand current. As a result the effect of load distribution between battery and fuel cell was evaluated and the operation time of each propulsion system was able to be estimated exactly.

• Toxicological Research
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• v.31 no.1
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• pp.11-16
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• 2015

Our body's immune system has defense mechanisms against pathogens such as viruses and bacteria. Immune responses are primarily initiated by the activation of toll-like receptors (TLRs). In particular, TLR4 is well-characterized and is known to be activated by gram-negative bacteria and tissue damage signals. TLR4 requires myeloid differentiation factor 2 (MD2) as a co-receptor to recognize its ligand, lipopolysaccharides (LPS), which is an extracellular membrane component of gram-negative bacteria. Gambogic acid is a xanthonoid isolated from brownish or orange resin extracted from Garcinia hanburyi. Its primary effect is tumor suppression. Since inflammatory responses are related to the development of cancer, we hypothesized that gambogic acid may regulate TLR4 activation. Our results demonstrated that gambogic acid decreased the expression of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6, IL-12, and $IL-1{\beta}$) in both mRNA and protein levels in bone marrow-derived primary macrophages after stimulation with LPS. Gambogic acid did not inhibit the activation of Interferon regulatory factor 3 (IRF3) induced by TBK1 overexpression in a luciferase reporter gene assay using IFN-${\beta}$-PRD III-I-luc. An in vitro kinase assay using recombinant TBK1 revealed that gambogic acid did not directly inhibit TBK1 kinase activity, and instead suppressed the binding of LPS to MD2, as determined by an in vitro binding assay and confocal microscopy analysis. Together, our results demonstrate that gambogic acid disrupts LPS interaction with the TLR4/MD2 complex, the novel mechanism by which it suppresses TLR4 activation.

• Journal of Applied Biological Chemistry
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• v.63 no.2
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• pp.137-145
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• 2020

Oxidative stress is one of the pathogenic mechanisms of various neurodegenerative diseases, such as Alzheimer's disease. Neuroglia, the most abundant cells in the brain, is thought to play an important role in the antioxidant defense system and neuronal metabolic support against neurotoxicity and oxidative stress. We investigated the protective effect of paeoniflorin (PF) against oxidative stress in C6 glial cells. Exposure of C6 glial cells to hydrogen peroxide (H₂O₂, 500 μM) significantly decreased cell viability and increased amounts of lactate dehydrogenase (LDH) release, indicating H₂O₂-induced cellular damage. However, treatment with PF significantly attenuated H₂O₂-induced cell death as shown by increased cell survival and decreased LDH release. The H₂O₂-stimulated reactive oxygen species production was also suppressed, and it may be associated with improvement of superoxide dismutase activity by treatment with PF. In addition, an increase in ratio of Bcl-2/Bax protein expression was observed after treatment with PF. In particular, the down-stream of the apoptotic signaling pathway was inhibited in the presence of PF, mostly by reduction of cleaved-poly ADP ribose polymerase, cleaved caspase-3, and -9 protein expression. Furthermore, H₂O₂-induced phosphorylation of c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2 was attenuated by treatment with PF. Taken together, neuroprotective effect of PF against oxidative stress probably result from the regulation of apoptotic pathway in C6 glial cells. In conclusion, our findings suggest that PF may be a potent therapeutic agent for neurodegenerative disorders.

• Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.5.1-5.8
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• 2018

Stimulator of interferon gene (STING) is induced by various inflammatory agents, such as lipopolysaccharide and microbial pathogens, including virus and bacteria. In this study, we obtained a full-length cDNA of a STING homolog from olive flounder using rapid amplification of cDNA ends PCR technique. The full-length cDNA of Paralichthys olivaceus STING (PoSTING) was 1442 bp in length and contained a 1209-bp open reading frame that translated into 402 amino acids. The theoretical molecular mass of the predicted protein sequence was 45.09 kDa. In the PoSTING protein, three transmembrane domains and the STING superfamily domain were identified as characteristic features. Quantitative real-time PCR revealed that PoSTING expressed in all the tissues analyzed, but showed the highest level in the spleen. Temporal expression analysis examined the significantly upregulated expression of PoSTING mRNA after viral hemorrhagic septicemia virus (VHSV) stimulation. In contrast, no significant changes in the PoSTING expression were detected in Edwardsiella tarda-challenged group compared to the un-injected control. The expression of P. olivaceus type I interferon (PoIFN-I) was also highly upregulated upon VHSV challenge. These results suggest that STING might be involved in the essential immune defense against viral infection together with the activation of IFN-I in olive flounder.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.7
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• pp.1079-1083
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• 2015

Activating macrophage cells play an important role in the host immune defense system. In this paper, immuno-modulatory activities of polysaccharides separated from Jubak (JPS) in macrophage cells were investigated. Immuno-modulatory activities were estimated based on cell proliferation, nitric oxide (NO) and cytokine production, degree of mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-${\kappa}B$ phosphorylation in RAW264.7 macrophage cells. JPS (62.5 to $250{\mu}g/mL$) did not induce a cytotoxic event. Additionally, NO and proinflammatory cytokines (tumor necrosis factor-${\alpha}$ and interleukin-6) production significantly increased in a dose-dependent manner. Similarly, phosphorylation of MAPKs and NF-${\kappa}B$ increased upon JPS treatment. Therefore, our results suggest that polysaccharides separated from Jubak can induce macrophage activation through MAPK and NF-${\kappa}B$ signaling and induction of Th1 polarization.

• Journal of the Korea Institute of Military Science and Technology
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• v.19 no.2
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• pp.195-201
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• 2016

An experimental study has been found the air guide grooves for reducing drag. When a bullet is fired and move in the air, the drag is generated. The vortex which is one of the types of drag hinders the movement of the bullet. To solve this phenomenon, cut a negative grooves that we are called the air guiding grooves at the back of bullet. The grooves bullet has identified that the drag compared to conventional ammunition(KM80 and K193) is reduced to 4.480 and 4.054 : 10 % through a Finite Analysis Program($Ansys^{TM}$). Even pressure center was retreating 0.72 % compared to a Bullet(KM80 and K193). Effect obtained with these results is the accuracy of the grooves bullet in a shooting test was improved by over 32 %(KM80: 2.86, air guide grooves : 1.94) compared to conventional ammunition(KM80 and K193). In addition, muzzle velocity is increased 73 m/s. This is expected to be extended the velocity and effective range of bullet. Also, the velocity of the grooves bullet is increased when moving in the air while the velocity of the bullet(KM80 and K193) is reduced. The gas ejected from the muzzle to be balanced and stable flight of the Bullet. Given these effects, we can reckon the air guide grooves have positive influence.

• Journal of the Korea Society for Simulation
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• v.27 no.1
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• pp.15-24
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• 2018

High Level Architecture(HLA) for modeling and simulation is an international technical standard to achieve simulation interoperability and reusability. Building federation through the interworking of federates is being used in various fields and is being used in KR(Key Resolve), UFG(Ulchi-Freedom Guardian) as a representative in domestic military. Currently, United States Department of Defense emphasizes the HLA compliance test, which confirms the compliance of Federate by using HLA. However, due to the limitations of testing tools, an HLA compliance test of DoD is not able to perform some of the functional tests on HLA/RTI services such as federation storage/recovery services and also exclude inspection of exchange data between federates. To solve these problems, this paper proposes an HLA compliance testing system of ROK suitable for domestic situations. This will enable effective HLA compliance testing and improve interoperability between federates.