• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.57-58
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• 2000

Production of dye-decolorizing enzyme was investigated with the cultivation of Geotrichum candidum Dec1 (abbreviated to Dec1) using molasses as a cheap raw materials. Molasses was found to stimulate the enzyme production as well as a role of carbon source, because the production increased with molasses content up to 50 g/L. However, the severe inhibition of dye-decolorizing enzyme activity was observed even at low concentration of molasses 10 g/L when purified decolorizing peroxidase was used. Its inhibitory effect was reduced through the cultivation of Dec1. The fractions of molasses separated by a gel chromatography showed the different degrees of inhibition. As a way to reduce the inhibitory effect, the dilution of culture broth was examined, and the total decolorizing activity for Reactive blue 5 increased 7 times as much as that of original culture broth by 30 times dilution. On the basis of result, we proposed a process scheme which can fully utilize both positive and negative effect of molasses in dye-decolorizing process using molasses.

• Journal of the Korean Society of Fashion and Beauty
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• v.4 no.4 s.10
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• pp.81-86
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• 2006

Numerous novel ingredients have been introduced for the hight functionality of whitening cosmetics. Tough the preliminary research, we have found water extracts Bombyx mori have high whitening efficacy. The results of the research for the whitening effect of Bombyx Mori L. are as follow 1. Bombyx Mori L. inhibited concentration dependently the generation of melanin increased by the stimulation of $\alpha$-MSH and protoporphyrin IX, and $IC_{50}$ value was 8.3, 9.2 ${\mu}M$ respectively. 2. Melanin increased by the stimulation of $\alpha$-MSH and protoporphyrin IX was five to seven times superior in the inhibiting effect, compared with kojic acid used as positive control group. 3. Bombyx Mori L. did not have a decolorizing effect on melanin already generated. 3. Bombyx Mori L. was observed to have toxicity of over 100 ${\mu}M$ for the mouse melanoma B16 cells. Therefore, These results suggest that water extracts of Bombyx mori have inhibitory activity against mushroom tyrosinase and inhibitory activity of melanin synthesis in B16 melanoma cells in vitro.

• Fashion & Textile Research Journal
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• v.8 no.3
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• pp.326-330
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• 2006

The results of the research for the whitening effect and functional machanism of 1-(2-cyclohexylmethoxy-6-hydroxyphenyl)-3-(4-hydroxymethylphenyl)-propenone are as follow : 1. Propenone inhibited concentration-dependently the generation of melanin increased by the stimulation of ${\alpha}$-MSH and protoporphyrin IX, and $IC_{50}$ value was six to eight ${\mu}M$. This was five to seven times superior in the inhibiting effect, compared with kojic acid used as positive control group. 2. Propenone did not have a decolorizing effect on melanin already generated. 3. Propenone was observed to have toxicity of over $100{\mu}M$ for the mouse melanoma B16 cells.

• KSBB Journal
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• v.15 no.1
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• pp.66-71
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• 2000

The results for decolorization of various dyes by Geotrichum candidum (KCTC 6195) showed that optimal initial pH, temperature and glucose concentration were 6, $30^{\circ}C$, and 30g/L. Light had no effect on the cell growth and decolorization efficiency. All the dyes - dispersive dyes, acid dyes and reactive dyes - used on the solid medium were also decolorized in a liquid medium, although the decolorizing rates varies depending on the dye structure. An energy source was essential for cell growth or decolorization because textile dyes did not support growth. The percentage of decolorization of Acid orange 10 was shown to be 91% for initial conc. 100ppm and 84% for initial conc. 500ppm. The biomass could adsorb the dyes such as Acid red 1;19.8%, Acid red 88; 73%, Acid orange 10; 12.1% Reactive blue 19; 14.6%. The dye removal was due to the sorption of dye to the fungal biomass as well as some extracellular enzymes. Color removal was enhanced up to 97% within 3 days by the addition of glucose after 2 days incubation.

• Journal of the Korean Wood Science and Technology
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• v.26 no.3
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• pp.41-47
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• 1998

Some white-rot fungi, screened at the Laboratory of Forest Products Microbiological Chemistry, Chungbuk National University were cultured and added the inducer of laccase enzyme, 2,5-xylidine. The fungi named by CB-13, CB-20, CB-99, CB-100 and CB-123 strains showed positive results in the decolorization of aromatic compounds, carminic acid and Rhemazol brilliant blue R. Concerned to the inducing effect of 2,5-xylidine on laccase activity, CB-20, CB-100 and CB-123 strains showed very high activity by addition of 2,5-xylidine, whilst CB-13, CB-99 and CB-124 strains produced relatively high laccase enzymes, regardless of inducer addition. There were no any laccase activities on CB-25, CB-64 and CB-139, even in addition of inducer. It is confirmed that some screened fungi have decolorizing ability on aromatic compounds, carminic acid and Rhemazol brilliant blue R. Also, the addition of inducer, 2,5-xylidine, has increased the activity of laccase enzyme which is secreted from some white-rot fungi.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.2
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• pp.77-86
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• 1990

The residue after hot water extraction of blue crab, Portunus trituberculata, was hydrolyzed for utilizing the byproducts as seasonings. The acid(5N HCl) hydrolyzates were then neutralized with $Na_2CO_3$, 5N NaOH or 5N NaOH hydrolyzate, while the alkali hydrolyzates (5N NaOH) were also neutralized with 5N HCl or 5N HCl hydrolyzate. The total nitrogen and formol nitrogen contents increased, and the platability of the hydrolyzates was also enhanced by neutralization. The released amino acid contents from the neutralized hydrolyzates with $Na_2CO_3$, 5N NaOH and 5N NaOH hydrolyzate were $2,274mg\%,\;2,105.0mg\%$ and $2,683.5mg\%$, respectively. Amino acid contents from the neutralized hydrolyzates with 5N HCl and 5N HCl hydrolyzate were $1,352.5mg\%$ and $2,498.8mg\%$, respectively. In the decolorization of hydrolyzates using decolorization agent, powdered active carbon showed good decolorizing effect. Powdered active carbon decreased total nitrogen and formol nitrogen contents in direct relationship to the increase in its concentration. The effective concentration of active carbon used as decolorization agent showed as $1\~2\%$ of the crab hydrolyzate. Salt contents could be decreased at 37 brix by desalination method such as the evaporation of the hydrolyzate contents.