• Korean Journal of Microbiology
• /
• v.17 no.4
• /
• pp.198-202
• /
• 1979

Three Pseudomonas spp. and one Zoogloea sp. which could docompose the furfural were isolated from the enriched undefined cultures of soil. In the decompositioin of furfural thyey demonstrated protocooperation and synergism, utilizing 2-furoic acid a certain sudstance fural was subject to complete oxidation, which resulted in decolorization by mutural interactions. The decomposition was more efficient in mixed cultures than in a single culture.

• Proceedings of the Korean Society of Life Science Conference
• /
• 2003.10a
• /
• pp.89-89
• /
• 2003

• Proceedings of the Korean Society of Life Science Conference
• /
• 2007.10a
• /
• pp.107.2-107.2
• /
• 2007

See Full Text

• Proceedings of the Korean Society of Life Science Conference
• /
• 2007.10a
• /
• pp.108.2-108.2
• /
• 2007

See Full Text

• Journal of Korean Society on Water Environment
• /
• v.20 no.2
• /
• pp.110-119
• /
• 2004

The combination of biological and physical/chemical technologies is a promising technique to reduce highly concentrated pollutants in livestock wastewater. It is suggested to treat livestock wastewater efficiently as follows: firstly, biodegradable organic matters, nitrogen and some of phosphorus should be removed by a biological treatment process and then residual non-biodegradable organic matters, color and phosphorus be eliminated by physicochemical technologies. In this study, therefore, the integrations of chemical coagulation, activated carbon adsorption, Fenton oxidation and ozonation were evaluated to provide appropriate post-treatment processes for biologically pre-treated livestock wastewater. After chemical coagulation followed by ozonation or Fenton oxidation process, the quality of treated wastewater could meet the discharge limit in Korea. However, a yellowish brown color still remained in the treated wastewater after a single method such as coagulation and Fenton oxidation was applied. The ozonation was found to be the most effective technology for the decolorization. Neither simple biological nor physicochemical treatment provides adequate decolorization and sufficient depletion of organics in livestock wastewater so far. Consequently, the integration of Fenton oxidation and ozonation with a biological treatment process is recommended to treat livestock wastewater in terms of removal efficiency.

• Journal of Mushroom
• /
• v.13 no.1
• /
• pp.63-67
• /
• 2015

White rot fungi produce lignin-degrading enzymes such as laccase, manganese peroxidase and lignin peroxidase. These extracellular oxidases efficiently degrade recalcitrant synthetic dyestuffs with diverse chemical structures. Here, we examined the activities of lignin-degrading enzymes in Trametes versicolor using triphenyl methane dyes, crystal violet (CV) and malachite green (MG). Both dyes were decolorized by T. versicolor in solid and liquid culture conditions. T. versicolor decolorized MG more quickly than CV in both conditions. Among three ligninolytic enzymes, laccase was most abundantly found in the decolorization processes of CV and MG. However, higher activity of laccase was needed to degrade CV than MG. The much less activity of MnP was also detected. But the increase of MnP activity was well corresponded to the decolorization efficiency of CV, suggesting the involvement of MnP in CV degrading process. However, its role in the degradation process of MG is supposed to be subsidiary to laccase.

• Journal of Environmental Science International
• /
• v.20 no.7
• /
• pp.899-905
• /
• 2011

In order to develop bacterial cellulose (BC) with antimicrobial activity against pathogenic microorganisms, silver and chitosan were incorporated into BC, respectively. Experiment results showed that antimicrobial activity against pathogenic microorganisms was improved with increasing silver concentration. Chitosan also showed a direct proportion between its concentration and antimicrobial activity. These results suggest that antimicrobial effects of BC using silver and chitosan are well proven to be effective. We also tested the stainability of BC with natural colorant for the application of food industry. Stainability of BC was enhanced with increasing natural colorant concentration. Decolorization of BC stained was observed by dipping it into distilled water with one hour-intervals. As a result, there was no significant difference. Combination of natural colorant-stainability and antibiosis of BC is expected to be useful in making colored antibiotic BC in various industrial application areas, considering its antimicrobial activity, high stainability and low decolorization tendency.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.6
• /
• pp.803-813
• /
• 2015

The growth of Irpex lacteus F17 and manganese peroxidase (MnP) production in a selfdesigned tray bioreactor, operating in solid-state conditions at a laboratory scale, were studied. The bioreactor was divided into three layers by three perforated trays. Agroindustrial residues were used both as the carrier of bound mycelia and as a nutrient medium for the growth of I. lacteus F17. The maximum biomass production in the bioreactor was detected at 60 h of fermentation, which was consistent with the CO₂ releasing rate by the fungus. During the stationary phase of fungal growth, the maximum MnP activity was observed, reaching 950 U/l at 84 h. Scanning electron microscopy images clearly showed the growth situation of mycelia on the support matrix. Furthermore, the MnP produced by I. lacteus F17 in the bioreactor was isolated and purified, and the internal peptide sequences were also identified with mass spectrometry. The optimal activity of the enzyme was detected at pH 7 and 25℃, with a long half-life time of 9 days. In addition, the MnP exhibited significant stability within a broad pH range of 4-7 and at temperature up to 55℃. Besides this, the MnP showed the ability to decolorize the polymeric model dye Poly R-478 in vitro.

• Journal of Korean Society of Environmental Engineers
• /
• v.30 no.8
• /
• pp.823-830
• /
• 2008

The feasibility study for the application of the photoelectrocatalytic decolorization of Rhodamine B(RhB) was performed in the slurry photoelectrochemical reactor with powder TiO$_2$. The photoelectrocatalytic process was consisted of powder TiO$_2$, Pt electrode and three 8 W UV-C lamps. The effects of operating conditions, such as current, electrolyte, air flow rate and electrode material were evaluated. The experimental results showed that optimum TiO$_2$ dosage and current in photoelectrocatalytic process were 0.4 g/L and 0.02 A, respectively. It was found that the RhB could be degraded more efficiently by this photoelectrocatalytic process than the sum of the two individual oxidation processes(photocatalytic and electrolytic process). It demonstrated a synergetic effect between the photo- and electrochemical catalysis. Photoelectrocatalytic process was affected to air flow rate and optimum air flow rate was 2 L/min. The electrode material and NaCl effect of decolorization of RhB were not significant within the experiment conditions.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.11
• /
• pp.1540-1548
• /
• 2012

Manganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAE-cellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and $35^{\circ}C$, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0, and at temperatures of up to $35^{\circ}C$ at a pH of 4.5 for 1h. The $K_m$ and $V_{max}$ values for the substrate phenol red were found to be $8{\mu}m$ and 111.14 U/mg of protein, respectively. The MnP also oxidized other substrates such as guaiacol, DMP, and veratryl alcohol. Sodium azide, EDTA, SDS, $Cu^{2+}$, and $Fe^{2+}$, at 1-5 mM, strongly inhibited enzyme activity, whereas $Ca^{2+}$ and $Zn^{2+}$ increased enzyme activity. The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.