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Viator vitreocola gen. et sp. nov. (Stylonematophyceae), a new red alga on drift glass debris in Oregon and Washington, USA

  • Hansen, Gayle I.;West, John A.;Yoon, Hwan Su;Goodman, Christopher D.;Goer, Susan Loiseaux-de;Zuccarello, Giuseppe C.
    • ALGAE
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    • 제34권2호
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    • pp.71-90
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    • 2019
  • A new encrusting red alga was found growing abundantly on glass debris items that drifted ashore along the coasts of Oregon and Washington. These included discarded fluorescent tubes, incandescent light bulbs, capped liquor bottles, and ball-shaped fishing-net floats. Field collections and unialgal cultures of the alga revealed that it consisted of two morphological phases: a young loosely aggregated turf and a mature consolidated mucilaginous crust. The turf phase consisted of a basal layer of globose cells that produced erect, rarely branched, uniseriate to multiseriate filaments up to $500{\mu}m$ long with closely spaced cells lacking pit-plugs. These filaments expanded in size from their bases to their tips and released single cells as spores. At maturity, a second phase of growth occurred that produced a consolidated crust, up to $370{\mu}m$ thick. It consisted of a basal layer of small, tightly appressed ellipsoidal-to-elongate cells that generated a mucilaginous perithallial matrix containing a second type of filament with irregularly spaced cells often undergoing binary division. At the matrix surface, the original filaments continued to grow and release spores but often also eroded. Individual cells, examined using confocal microscopy and SYBR Green staining, were found to contain a central nucleus, a single highly lobed peripheral chloroplast without a pyrenoid, and numerous chloroplast nucleoids. Morphological data from field and culture isolates and molecular data (rbcL, psbA, and SSU) show that this alga is a new genus and species which we name Viator vitreocola, "a traveller on glass."

Denoise of Astronomical Images with Deep Learning

  • Park, Youngjun;Choi, Yun-Young;Moon, Yong-Jae;Park, Eunsu;Lim, Beomdu;Kim, Taeyoung
    • 천문학회보
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    • 제44권1호
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    • pp.54.2-54.2
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    • 2019
  • Removing noise which occurs inevitably when taking image data has been a big concern. There is a way to raise signal-to-noise ratio and it is regarded as the only way, image stacking. Image stacking is averaging or just adding all pixel values of multiple pictures taken of a specific area. Its performance and reliability are unquestioned, but its weaknesses are also evident. Object with fast proper motion can be vanished, and most of all, it takes too long time. So if we can handle single shot image well and achieve similar performance, we can overcome those weaknesses. Recent developments in deep learning have enabled things that were not possible with former algorithm-based programming. One of the things is generating data with more information from data with less information. As a part of that, we reproduced stacked image from single shot image using a kind of deep learning, conditional generative adversarial network (cGAN). r-band camcol2 south data were used from SDSS Stripe 82 data. From all fields, image data which is stacked with only 22 individual images and, as a pair of stacked image, single pass data which were included in all stacked image were used. All used fields are cut in $128{\times}128$ pixel size, so total number of image is 17930. 14234 pairs of all images were used for training cGAN and 3696 pairs were used for verify the result. As a result, RMS error of pixel values between generated data from the best condition and target data were $7.67{\times}10^{-4}$ compared to original input data, $1.24{\times}10^{-3}$. We also applied to a few test galaxy images and generated images were similar to stacked images qualitatively compared to other de-noising methods. In addition, with photometry, The number count of stacked-cGAN matched sources is larger than that of single pass-stacked one, especially for fainter objects. Also, magnitude completeness became better in fainter objects. With this work, it is possible to observe reliably 1 magnitude fainter object.

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움직임 상상 기반 뇌-컴퓨터 인터페이스를 위한 운동 심상, 실행, 관찰 뇌파 비교 분석 (A Comparative Analysis of Motor Imagery, Execution, and Observation for Motor Imagery-based Brain-Computer Interface)

  • 권다은;황민주;권지현;신예은;안민규
    • 대한의용생체공학회:의공학회지
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    • 제43권6호
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    • pp.375-381
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    • 2022
  • Brain-computer interface (BCI) is a technology that allows users with motor disturbance to control machines by brainwaves without a physical controller. Motor imagery (MI)-BCI is one of the popular BCI techniques, but it needs a long calibration time for users to perform a mental task that causes high fatigue to the users. MI is reported as showing a similar neural mechanism as motor execution (ME) and motor observation (MO). However, integrative investigations of these three tasks are rarely conducted. In this study, we propose a new paradigm that incorporates three tasks (MI, ME, and MO) and conducted a comparative analysis. For this study, we collected Electroencephalograms (EEG) of motor imagery/execution/observation from 28 healthy subjects and investigated alpha event-related (de)synchronization (ERD/ERS) and classification accuracy (left vs. right motor tasks). As result, we observed ERD and ERS in MI, MO and ME although the timing is different across tasks. In addition, the MI showed strong ERD on the contralateral hemisphere, while the MO showed strong ERD on the ipsilateral side. In the classification analysis using a Riemannian geometry-based classifier, we obtained classification accuracies as MO (66.34%), MI (60.06%) and ME (58.57%). We conclude that there are similarities and differences in fundamental neural mechanisms across the three motor tasks and that these results could be used to advance the current MI-BCI further by incorporating data from ME and MO.

Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods

  • Renata Aqel de Oliveira;Theodoro Weissheimer;Gabriel Barcelos So ;Ricardo Abreu da Rosa ;Matheus Albino Souza;Rodrigo Goncalves Ribeiro ;Marcus Vinicius Reis So
    • Restorative Dentistry and Endodontics
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    • 제48권1호
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    • pp.1.1-1.11
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    • 2023
  • Objectives: This study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols. Materials and Methods: Sixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3). Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student's t-test were used for data analysis, with a significance level of 5%. Results: The NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019). Conclusions: The NaOCl penetration depth was similar in groups with root canal preparation. Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.

What do we know about uncommon complications associated with third molar extractions? A scoping review of case reports and case series

  • Naichuan Su;Sana Harroui;Fred Rozema;Stefan Listl;Jan de Lange;Geert J.M.G. van der Heijden
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제49권1호
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    • pp.2-12
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    • 2023
  • The current study aimed to explore the types and frequencies of uncommon complications associated with third molar extractions based on a scoping review of case reports and case series. The study used an electronic literature search based on PubMed and Embase up to March 31, 2020, with an update performed on October 22, 2021. Any case reports and case series that reported complications associated with third molar extractions were included. The types of complications were grouped and the main symptoms of each type of complication were summarized. A total of 51 types of uncommon complications were identified in 248 patients from 186 studies. Most types of complications were post-operative. In the craniofacial and cervical regions, the most frequent complications included iatrogenic displacement of the molars or root fragments in the craniofacial area, late mandibular fracture, and subcutaneous emphysema. In other regions, the most frequent complications include pneumomediastinum, pneumorrhachis, pneumothorax, and pneumopericardium. Of the patients, 37 patients had life-threatening uncommon complications and 20 patients had long-term/irreversible uncommon complications associated with third molar extractions. In conclusion, a variety of uncommon complications associated with third molar extractions were identified. Most complications occurred in the craniofacial and cervical regions and were mild and transient.

Effect of post space preparation drills on the incidence of root dentin defects

  • Thaise Ayres Bezerra Zuli ;Orlando Aguirre Guedes ;Gislaine Figueiredo Zarza Arguello Goncalves;Aurelio Rosa da Silva Junior ;Alvaro Henrique Borges ;Andreza Maria Fabio Aranha
    • Restorative Dentistry and Endodontics
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    • 제45권4호
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    • pp.53.1-53.11
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    • 2020
  • Objectives: This study investigated the incidence of root dentin defects after the use of different post space preparation (PSP) drills. Materials and Methods: Seventy-two bovine incisors were selected and obtained 14-mm-long root sections. Twelve roots served as controls with no intervention (G1). The 60 root canals remaining were instrumented using the crown-down technique with the ProTaper Next system and obturated using the lateral condensation technique. Specimens were randomly distributed into 5 groups (n = 12) according to the operative steps performed: G2, root canal instrumentation and filling (I+F); G3, I+F and PSP with Gates-Glidden drills; G4, I+FI+F and PSP with Largo-Peeso reamers; G5, I+F and PSP with Exacto drill; and G6, I+F and PSP with WhitePost drill. Roots were sectioned at 3, 6, 9, and 12 mm from the apex, and digital images were captured. The presence of root dentin defects was recorded. Data were analyzed by the χ2 test, with p < 0.05 considered to indicate statistical significance. Results: Root dentin defects were observed in 39.6% of the root sections. No defects were observed in G1. G5 had significantly more cracks and craze lines than G1, G2, and G3 (p < 0.05), and more fractures than G1, G2, G3, and G4 (p < 0.05). When all root sections were analyzed together, significantly more defects were observed at the 12-mm level than at the 3-mm level (p < 0.05). Conclusions: PSP drills caused defects in the root dentin. Gates-Glidden drills caused fewer root defects than Largo-Peeso reamers and Exacto drills.

시베리아 철갑상어(Acipenser baerii) 트랜스페린(Transferrin) 유전자의 발생 배 및 초기 자어에서의 발현 특징 (Expression Characteristics of Transferrin Gene During Embryo Development and Prolarval Ontogeny in Siberian Sturgeon Acipenser baerii)

  • 김은정;남윤권
    • 한국수산과학회지
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    • 제57권1호
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    • pp.41-52
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    • 2024
  • Serotransferrin cDNA from the Siberian sturgeon Acipenser baerii was isolated and its expression patterns during early life intervals were characterized. It contained an ORF encoding a 708-aa-long polypeptide, including a 19-aa signal peptide. Bioinfomatic analysis and 3D modeling indicated a typical bi-lobal structure with conserved iron-coordinating residues. During embryonic development, the potential transition of maternally provisioned transcripts to zygotically de novo transcribed ones occurred around blastula stage. The transferrin mRNA levels peaked at stages responsible for pronephros, heart and erythropoietic component differentiation. After hatching, the transferrin mRNA expression gradually increased at early ontogenic phases (0 to 3 DPH) corresponding to the periods in which prolarvae exhibited increased blood circulation and liver differentiation. The expression decreased at subsequent stages in which prolarvae exhibited benthic movement. The tissue distribution assay indicated liver-predominant expression at fingerling stage. From the microinjection-based challenge with Aeromonas hydrophila at day-0 and day-7, the transcriptional response was modulated toward upregulation, in which the amounts induced at 6, 12 and 24 HPI were greater in prolarvae injected at day-7 than at day-0. Therefore, transferrin plays important roles in both early development and host protective responses to pathogens in the Siberian sturgeon.

Clinical Outcomes of Intracardiac Echocardiography-Guided Contrast Agent-Free Cryoballoon Ablation in Atrial Fibrillation Patients With Renal Insufficiency

  • Dong Geum Shin;Jinhee Ahn;Sang Hyun Park;Sang-Jin Han;Hong Euy Lim
    • Korean Circulation Journal
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    • 제54권3호
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    • pp.113-123
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    • 2024
  • Background and Objectives: Previous studies have reported an association between impaired renal function and poor outcomes after radiofrequency catheter ablation in patients with atrial fibrillation (AF). However, outcomes of cryoballoon ablation (CBA) in patients with renal insufficiency are not fully elucidated. This study aimed to compare outcomes of CBA in AF patients with chronic kidney disease (CKD) versus those without CKD and to assess changes in renal function over 12 months following CBA. Methods: A total of 839 patients (65.1% with non-paroxysmal AF [PAF]) who underwent de novo CBA were prospectively enrolled. We divided patients into two groups based on creatinine clearance rate (CCr) and performed intracardiac echocardiography (ICE)-guided contrast agent-free CBA. Results: In comparison with patients without CKD (CCr >50, n=722), those with CKD (CCr ≤50, n=117) were older and predominantly female, had a lower body mass index, and showed a higher prevalence of heart failure and hypertension. Mean CHA2DS2-VAS score was significantly higher in CKD group than in non-CKD group. Procedure-related complications were not significantly different between two groups. During a mean follow-up period of 25.4±11.9 months, clinical recurrence occurred in 182 patients (21.7%) and not significantly different between two groups. In multivariate analysis, non-PAF and left atrial size were independent predictors of AF recurrence. CCr levels significantly improved over 12 months after CBA in CKD group. Conclusions: ICE-guided contrast-agent-free CBA showed comparable long-term clinical outcomes without increasing procedure-related complications and improvement of renal function over 12 months following CBA in AF patients with CKD.

Enabling Effective Implementation of Occupational Safety and Health Interventions

  • Gaia Vitrano;Davide Urso;Guido J.L. Micheli;Armando Guglielmi;Diego De Merich;Mauro Pellicci
    • Safety and Health at Work
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    • 제15권2호
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    • pp.213-219
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    • 2024
  • Background: The design, implementation, and evaluation are three important stages of occupational safety and health (OSH) interventions. Historically, there has been a tendency to prioritize implementation, often neglecting detailed design and rigorous outcome evaluation. Currently, much has changed, and contemporary approaches recognize the interdependence of these stages, considering them integral to the success of any intervention. This work presents a comprehensive procedure for implementing interventions, not only to ensure short-term effectiveness but also their long-term sustainability through continuous monitoring. The focus is on a national OSH project introducing a near-miss management system (NMS) in Italy. Methods: Initial meetings were convened among project partners, complemented by interviews with diverse stakeholders, to plan implementation steps and test the NMS. Tailored questionnaires were designed for diverse stakeholder groups - initial promoters, company managers and employers, and employees - facilitating targeted implementation, and three case studies were started in Italian regions to assess the structured implementation, involving intervention promoters and collaborating companies. Results: The primary outcome is the development of practical tools, specifically three questionnaires, which are considered valuable for establishing an effective human-centered implementation strategy, meticulously designed to facilitate ongoing monitoring of processes and continual enhancement of instruments intended for NMS integration within companies. Conclusions: This work lays the foundation for successful NMS implementation in Italy and, although the outlined procedure had specific objectives, it also provides valuable insights applicable in enhancing the effectiveness and sustainability of interventions across diverse contexts. It underscores the importance of comprehensive planning, stakeholder engagement, and continuous evaluation in achieving lasting OSH interventions.

Model for end-stage liver disease-3.0 vs. model for end-stage liver disease-sodium: mortality prediction in Korea

  • Jeong Han Kim;Yong Joon Cho;Won Hyeok Choe;So Young Kwon;Byung-Chul Yoo
    • The Korean journal of internal medicine
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    • 제39권2호
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    • pp.248-260
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    • 2024
  • Background/Aims: The model for end-stage liver disease (MELD) serves as an indicator for short-term mortality among patients diagnosed with liver cirrhosis (LC) and is used to prioritize patients for liver transplantation. In 2021, the updated version of MELD, MELD-3.0, was introduced to improve the accuracy of the mortality prediction of MELD. Therefore, this study aimed to compare the efficacy of MELD 3.0 and MELD-Na in predicting mortality among Korean patients with LC. Methods: A retrospective review was conducted using the medical records of patients diagnosed with LC who were admitted to Konkuk University Hospital From 2011 to 2021. The study calculated the predictive values of MELD-Na and MELD-3.0 for 3- and 6-months mortality using the area under the receiver operating curve (AUROC) and compared the results using the DeLong test. Results: Of the 3,034 patients enrolled in the study, 339 (11.2%) died within 3 months and 421 (14.4%) died within 6 months. The AUROCs values for predicting 3 months mortality were 0.846 for MELD-Na and 0.851 for MELD-3.0. The corresponding AUROC values for predicting 6 months mortality were 0.843 for MELD-Na and 0.848 for MELD-3.0. MELD-3.0 exhibited better discrimination ability than MELD-Na for both 3 (p = 0.03) and 6 months mortality (p = 0.01). Conclusions: Our study found a significant difference between the performance of MELD-3.0 and MELD-Na in Korean patients with LC.