• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.86-92
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• 2020

This study was conducted to analyze ripening characteristics of the seed according to days after flowering. The seeds were harvested on land located in the Department of Herbal Crop Research in Rural Development Administration in 2019. Seed weight and germination rate were investigated according to days after flowering and the embryo:seed ratio was examined during the fruiting process. The results showed that the weight increased significantly by the days after flowering at each inflorescence and the seed began to germinate at different time. Further, given the embryo:seed ratio, we found that embryo continue to grow in the seed. Because Angelica acutiloba (Siebold & Zucc.) Kitagawa bloom in various inflorescences, the stage of embryonic development of the seeds can affect the germination of seed. Based on our results, the key seed harvest period for good seed is 50 to 70 days after flowering.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.505-511
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• 1994

Seeds of Rigitaeda pine tree(Pinus rigida${\times}$P. taeda) was primed with polyethylene glycol(PEG-6000) under different PEG concentrations, treatment period, and temperatures to test uniformity of germination. Coated seeds and PEG treated seeds were sown to compare germination, emergence, establishment, and seedling growth. The results obtained are summarized as follows : 1. As the concentration of PEG increased, as total germination percentage of pine tree was increased. Total germination percentage of pine tree seeds primed with PEG at 15 or $20^{\circ}C$ was better than the seeds treated at $10^{\circ}C$ compared to untreated seeds. 2. Germination rate was improved as primed period long and level of PEG concentration high. 3. Maximum germination rate was high with long PEG treatment period, and markedly improved when the seeds were primed at $20^{\circ}C$ 4. Priming with PEG at $20^{\circ}C$ for 12 days reduced time taken for germination. 5. Osmoconditioned seeds accelerated germination under drought condition with injurious effect of coated seeds by some chemicals. 6. There were much differences in establishment and dry matter production between drill and oversowing method, and primed seeds showed better performances than the coated seeds. Coating effects to seeds were better under drought soil condition rather than moderate moisture condition.

• Journal of Bio-Environment Control
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• v.23 no.1
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• pp.39-42
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• 2014

The objective of this study was to estimate cardinal temperatures for germination of lettuce (Lactuca sativar L.) using bilinear, parabolic, and beta distribution functions. Seeds of lettuce were germinated in a growth chamber at 7 constant temperatures: 10, 14, 16, 20, 24, 28, and $32^{\circ}C$. Four replicates of 100 seeds were placed on two layers of filter paper in a 9 cm petri-dish. Radicle emergence of 1 mm was scored as germination. The time course of germination was modeled using a logistic function. These minimum, optimum, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate against the temperature gradient. In bilinear function, minimum, optimum, and maximum temperatures were $7.9^{\circ}C$, $23.3^{\circ}C$, and $28.0^{\circ}C$, respectively. In parabolic function, minimum, optimum, and maximum temperatures were $9.7^{\circ}C$, $19.5^{\circ}C$, and $29.4^{\circ}C$, respectively. In beta distribution function, minimum, optimum, and maximum temperatures were $3.7^{\circ}C$, $20.7^{\circ}C$ and $32.0^{\circ}C$, respectively. Minimum, optimum, and maximum ranges of temperatures were $3.7{\sim}9.7^{\circ}C$, $19.5{\sim}23.3^{\circ}C$, and $28.0{\sim}32.0^{\circ}C$, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.123-128
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• 1986

The characteristics such as characters of seed, fruit and endosperm, development of embryo, dehisced and germination rate of seeds which harvested at 10 days intervals from 20 days to 60 days after pollination were determined to clarify the optimum time for seed production of ginseng. The sizes of length, width and thickness of fruits, seeds and endosperms investigated on 20 days after pollination were not largely different from those that harvested after 60 days. But the weights of fruits and seeds were increased along with the delay of harvesting time. The seeds which harvested from 30 to 60 days showed comparatively high dehisced and germinated rates, especially 40 days in dehisced rates and 50 days in germinated rates. The embryos were rapidly grown in early stage. Lengths of the embryo on 20 days after pollination were approximately grown to 30 ${\mu}{\textrm}{m}$. And after 30 days pollinated, embryos were not only more rapidly grown to 213 ${\mu}{\textrm}{m}$ but also cotyledon premodia were developed in this time and completely formed after 40 days.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.216-220
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• 1998

The objectives of this study were to evaluate the effects of some seed pretreatments on seed germination, emergence, growth and yield of Bupleurum falcatum L. Seed treatments included stratification in open-field during winter, washing with running water, low temperature storage, soaking into $H_2SO_4$ solution, and scarification. Germination increased 15% by stratification and 9% by washing treatment compared to non-treatment, control. It was suggested that washing treatment leached inhibiting substance, while stratification treatment leached inhibiting substance and promoted growth of immatured embryo. In the field mulched with rice straw, stratification and washing treatments were effective to shorten days to emergence by 31 and 21 days and to increase emergence rate by 7 and 5 %, respectively, compared with control. By these two treatments, root growth was improved, the number of harvested plants was increased considerably and dry root yield per ha were increased by 29 and 22 %, respectively, compared with control.

• Journal of Radiation Protection and Research
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• v.26 no.4
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• pp.409-415
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• 2001

To observe the stimulating effects of low dose gamma radiation on the germination and physiological activity of germinating seeds of old red pepper (Capsicum annuum L. cv Jokwang and cv. Hongkwang), seeds were irradiated at the dose of $2{\sim}50 Gy$. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage of induction. The germination rate at 7 days after sowing in Jokwang and Hongkwang cultivar was high as 74% and 11% at 4 Gy and 8 Gy irradiation group, respectively. The seedling height of Jokwang cultivar was noticeably high at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group. The protein contents of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at the late stage of induction and that of Hongkwang cultivar at the early stage of induction. Catalase and peroxidase activities of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.760-767
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• 2016

Food waste (FW) has been recognized as a critical problem in Korea and many research was conducted to efficiently utilize or treat FW. Main purpose of this research was to evaluate a feasibility for producing fermented organic fertilizer with vegetable waste (VW). Three different organic materials (saw dust, coco peat, and waste mushroom media) were mixed with VW at the rate of 30, 40, 50% respectively. Total days of composting experiment were 35 days and each sub samples were collected at every 5 days from starting of composting. Result showed that inner temperature of composting was increased to $60{\pm}4^{\circ}C$ within 5~10 days depending on varied organic materials and mixing ratio. Among different treatment, the highest increase of inner temperature was observed when 30% of saw dust was mixed with VW. After finishing composting experiment, maturity of each compost was evaluated with solvita and germination test. Maturity index (MI) of each treatment was ranged between 5~7 indicating that manufactured fertilizer was curing or finished stage. Calculated germination index (GI) was at the range of 57.83~101.16 depending on organic materials and mixing ratio. Both MI and GI showed that manufactured fertilizer was met for fertilizer criteria while control (VW only) was not adequate for composting. Overall, VW can be utilized for making organic fertilizer mixing with saw dust, coco peat and more research should be conducted to make high quality of organic fertilizer with vegetable waste.

• Journal of Ginseng Research
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• v.20 no.1
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• pp.83-87
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• 1996

The effects of gibberilin ($GA_3$) on levels of endogenous indole-3-acetic acid (IAA) and zeatin in both fresh and stratified American ginseng (Panax quinquefolium) seeds were investigated. In our first experiment, the fresh seeds were stratified after soaked in 80 ppd $GA_3$ solution for 24 hours. We found that the IAA concentration in embryo increased by 50.7% and 82.1% respectively at the 120th day and the 188th day of stratification, and the zeatin concentration also increased by 3.8% and 51.6% respectively. In our second experiment, we treated the seeds after 134 days stratification with 80 ppm GA3 for 24 hours and then continued to stratify them. We found that the IAA concentration in embryo increased by 32.9% and 17.7% respectively at the 164th day and the 208th day of stratification while zeatin concentration increased by 22.7% and 30.6% respectively In our another experiment, we studied the effects of $GA_3$, abscislc acid (ABA) and GA, plus ABA on germination rate of seeds treated with these plant hormones during stratification. We found that when the stratified seeds whose ratio of embryo had reached 75% were treated with 80 ppm GA3 for 24 hours and then were allowed to be stratified for another 88 days, the weight and length of embryo (p < 0.05), and germination rate (p < 0.01) increased. In contrast, the 25 ppm ABA treated with for 24 hours was found to Inhibit the growth of embryo (p < 0.05) and reduce the germination rate (p < 0.05) . The experiment of combination treatment of $GA_3$ and ABA showed that $GA_3$ could relieve the inhibitory effects of the ABA on the development of the seeds.

• Korean Journal of Agricultural Science
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• v.10 no.1
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• pp.28-60
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• 1983

This study was carried out to define the effects of external factors including temperature, moisture, oxygen and light quality on the germination of sesame seeds and to investigate the change of major chemical constituents of seeds during germination. The results obtained are summarized as follows: 1. The average germination ratio was from 95.8% to 97.2% when it was tested every $5^{\circ}C$ intervals from $20^{\circ}C$ to $35^{\circ}C$ and no significant difference in germination ratio was found within $20^{\circ}C$ to $35^{\circ}C$. But the germination ratio dropped rapidly to 32.2% when seeds were germinated at $15^{\circ}C$ and the coefficient of variation become greater(77%) 2. The days required for germination ranged from 1.16 to 1. 64 at the temperatures of $35^{\circ}C$ to $25^{\circ}C$ and they were 3.07 and 10.4 at the temperatures of $20^{\circ}C$ and $15^{\circ}C$, respectively. 3. Considering the germination ratio and days needed, $15^{\circ}C$ was assumed to be the minimum temperature for germination practically and this temperature is recommended for testing low temperature tolerance of seed germination of sesame cultivars. 4. The varieties shown the highest low temperature tolerance were Shirogoma and Turkey. The next varieties shown some degree of low temperature germination were Suweon #29, Naebok and IS 58. The varieties with 70 to 80% of germination ratio were Maepo, Suweon #14, Kimpo, Moondeok, and Haenam. Among the 90 varieties tested, the varieties with comparatively high degree of low temperature tolerance were about 10%, and 70% of the low temperature tolerant varieties were domestic varieties. 5. At $12^{\circ}C$ the Shirogoma was the only variety which showed over 50% of germination ratio, 71.4% of the varieties showed less than 20% of germination ratio. When the temperature was raised to $27^{\circ}C$ 18 days after placement at $12^{\circ}C$ all the varieties showed over 90% of germination ratio within 2days. 6. The amounts of water imbibition needed for seed germination were 0.48 to 0.62 times of the seed dry weight at $25^{\circ}C$ and were significantly different among sesame cultivars. About 63% of water required for germination was imbibed in 2 hours after placement of seeds under the germination condition. 7. Under saturated moisture condition the average germination ratio was 0.42%. In the soil of which water potential was -0.4bar 64.8% of the seeds germinated and the most adequate soil water potential for sesame seed germination was about -0.4 to -5.5 bar. The germination ratio decreased as the soil water potential declined below -5.5 bar. 8. Six out of 10 varieties were not influenced by 5% of oxygen in air germination chamber, while varieties such as Yecheon, PI 158073, IS 103 and Euisangcheon showed 64 to 91% of germination under the 5% oxygen content. Under anaerobic condition, cotyledones were not emerged but only hypocotyl was emerged and elongated. The germination ratio of IS 103 decreased significantly under anaerobic condition. 9. When the seeds were dried for 24 hours after 12 hours imbibition of water, the seeds of Cheongsong did not lose their germination ability and 27.5% was germinated but Suweon #9 and Early Russian failed to germinate. However, the germination ratio of IS 103 decreased when the seed were dried 24 hours after 4 hours imbibition of water and the germination ability of IS 103 was maintained even though the seeds were dried for 24 hours after 24 hours imbibition of water. 10. During germination, sugar content of sesame seed increased rapidly and activity of ${\alpha}$-amylase increased gradually while starch content decreased significantly. The rates of increase in sugar content and enzyme activity and decrease in starch content were significantly lower at $15^{\circ}C$ compared with those at $25^{\circ}C$. 11. During germination of sesame seeds, lipid content in the seeds dropped rapidly and the activity of alkaline lipase increased significantly at early stage of germination. The rate of decrease in lipid content and increase in emzyme activity was lower at $15^{\circ}C$ than at $25^{\circ}C$. 12. Four out of 6 varieties were not affected in germination by light wave length. But Suweon #8 was inhibited in germination by 600-650nm. and IS 103 by 600 to 650nm and 500 to 550nm of light wave length. Suweon #8 showed high germination ratio under 650 to 760 nm and 500 to 560nm, and IS 103 under 400 to 470nm and complete darkness. 13. The germination ratios increased significantly in the seeds of which 1000 grain weight is heavier. When the seeds were placed at soil 4cm deep, Cheongsong and Early Russian failed to emerge their cotyledones, but Suweon #9 and IS 103 showed 32.5 and 50% cotyledone emergence, respectively. The extracts from sesame plant and soil where the sesame was cultivated previously did not affect in the-germination of sesame seeds. 14. The covering by black or transparent polyethylene films increased germination ratio compared with uncovered seeds. The covering was effective in shortening the days needed for germination and in improving the early seedling growth, number of capsules per plant and grain yield. Difference was not so seizable between the two polyethylene films but the transparent film appeared somewhat more effective than the black one. 15. Simcheon, Cheongsong. Suweon #9. PI 158073 and IS 103 showed lower rate of water absorbtion by seed during germination and Suweon #8, Suweon #26, Orotall and Euisangcheon showed high increase in seed weight after water absorbtion by seed.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.266-270
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• 2010

Corylopsis coreana is an endangered Korean native plants. This is one of the genus that have high ornamental value for flowering plants available for garden shrub, bonsai, and pot plants. In this study, the methods to encourage seed germination rate were investigated for its ornamental uses. The germination rate of Corylopsis coreana seeds stored under dry-cold condition was very low, 12%, 12%, 8%, and 10%after 40, 70, 85, and 100 days storage respectively. But the germination rate of Corylopsis coreana seeds stored under wet-cold condition was higher than these, 20%, 54%, 78%, and 96% after 40, 70, 85, and 100 days storage respectively. Dry seeds sowed directly without $GA_3$ treatment showed no germination regardless of storage type(cold or room temp.) or storage periods. On the other hand, the soaking treatment with $GA_3$ 50~500 ppm for 24 hours was very effective to increase the germination rate. The most effective $GA_3$ levels was different by storage type(cold or room temp.) and storage periods. But the effect of $GA_3$ was decreased by prolonging of the storage period. Soaking treatment with $Ca(NO_3)_2$ 5, 10, 20 mM, $KNO_3$ 5, 10, 20 mM for 24 hours showed no effect.