• Title/Summary/Keyword: Date screening

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Screening of Seed Disinfectant for Controlling Brown Leaf Blight in Alisma plantago Double Cropping after Early Rice

  • Shin, Dong-Young;Kwon, Byung-Sun;Lim, June-Taeg;Hyun, Kyu-Hwan;Lim, Jung-Mook;Seo, Young-Nam
    • Korean Journal of Plant Resources
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    • v.19 no.6
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    • pp.692-696
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    • 2006
  • This study was conducted to evaluate the effect of seed disinfectant, in control of brown leaf blight, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. Experimental plot was laid out in split plots design with three replications. The major seed disinfectants were benomyl Wp, 20%, Captan Wp, 50%, Triferine Ec, 17%, Etridia zole Ec, 25%, and Thioplant-mythyl Wp, 50%. Even though seed disinfectant treated had no effect on the growth and flowering date of Alisma plantago, dry root yield was increased largely with benomyl Wp, 20%, in seed disinfectant than in the other seed disinfectants and contorl. All seed disinfectants had no injury with standard dosage. But all seed disinfectants had slight injury in the double dosage level for the Alisma plantago. On the basis of yield, vegetative and disease paramerer, benomyl Wp (20%) ($100g/20{\ell}$) had shown superior performance, however, all the seed disinfectants are effective as compare to without treatment.

Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome

  • Tan, Hao;Mooij, Marlies J.;Barret, Matthieu;Hegarty, Pardraig M.;Harrington, Catriona;Dobson, Alan D.W.;O'Gara, Fergal
    • Journal of Microbiology and Biotechnology
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    • v.24 no.1
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    • pp.113-118
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    • 2014
  • Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.

Single-incision Laparoscopic Gastrectomy for Gastric Cancer

  • Lee, Yoontaek;Kim, Hyung-Ho
    • Journal of Gastric Cancer
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    • v.17 no.3
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    • pp.193-203
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    • 2017
  • The implementation of national cancer screening has increased the detection rates of early gastric cancer (EGC) in Korea. Since the successful introduction of laparoscopic gastrectomy for gastric cancer in the early 1990s, this technique has demonstrated improved short-term outcomes without compromising long-term oncologic results. It is associated with reduced pain, shorter hospitalization, reduced morbidity rates, better cosmetic outcomes, and equivalent mortality rates as those for open surgery. Laparoscopic gastrectomy improves patients' quality of life (QOL) and provides favorable prognosis. Single-incision laparoscopic gastrectomy (SILG) is one extremely minimally invasive method, theoretically offering improved cosmetic results, less postoperative pain, and earlier recovery after surgery than conventional multiport laparoscopic gastrectomy. In this context, SILG is thought to be an optimal method to promote and maximize patients' QOL in the acute postoperative phase. However, the technical difficulties of this procedure have limited its use. Since the first report describing single-incision distal gastrectomy in 2011, only 16 studies to date have evaluated SILG. Most of these studies have focused on the technical feasibility and safety of SILG because its long-term outcomes have not been reported. This article reviews the advantages and limitations of SILG.

Preselection and cloning of transgenic emb (유전자전환 수정란의 선별과 복제)

  • Lee, Hyo-Jong
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 1998.05a
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    • pp.12-28
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    • 1998
  • The technology of creating transgenic animals has a potential value in improving productivity and disease resistance of animals, gene therapy, drug pharming and production of model animals for certain diseases. Up to date, fairly low success rate of production of transgenic animals and a pronounced variability with respect to the expression of transgenes have been much observed. The mechanisms how to integrate the injected genes with a certain part of the genomes are unknown yet. Many techniques in gene transfer, beside microinjection, have been introduced and explored thus to improve the production efficiency of transgenic animals. In this article, the methods and efficiency of gene-transfer techniques, the detection and preselection of transgenes in embryos by PCR- and GFP-screenings and cloning of preselected transgenic embryos by nuclear transplantation are described and discussed. Some experimental results showed that the early screening and selection of integration of the injected gene with embryonic genome by polymerase chain reaction(PCR) and green fluorecence protein(GFP) were promising methods. Further, the application of nuclear transplantation technology to cloning and multiplication of the positively integrated genes in the cleaving embryos and embryonic cells will be beneficially used for the mass production of transgenic embryos and consequently improving the production efficiency in transgenic animals.

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Update on genetic screening and treatment for infertile men with genetic disorders in the era of assisted reproductive technology

  • Lee, Seung Ryeol;Lee, Tae Ho;Song, Seung-Hun;Kim, Dong Suk;Choi, Kyung Hwa;Lee, Jae Ho;Kim, Dae Keun
    • Clinical and Experimental Reproductive Medicine
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    • v.48 no.4
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    • pp.283-294
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    • 2021
  • A genetic etiology of male infertility is identified in fewer than 25% of infertile men, while 30% of infertile men lack a clear etiology, resulting in a diagnosis of idiopathic male infertility. Advances in reproductive genetics have provided insights into the mechanisms of male infertility, and a characterization of the genetic basis of male infertility may have broad implications for understanding the causes of infertility and determining the prognosis, optimal treatment, and management of couples. In a substantial proportion of patients with azoospermia, known genetic factors contribute to male infertility. Additionally, the number of identified genetic anomalies in other etiologies of male infertility is growing through advances in whole-genome amplification and next-generation sequencing. In this review, we present an up-to-date overview of the indications for appropriate genetic tests, summarize the characteristics of chromosomal and genetic diseases, and discuss the treatment of couples with genetic infertility by microdissection-testicular sperm extraction, personalized hormone therapy, and in vitro fertilization with pre-implantation genetic testing.

Nasal septal abscess with a dental origin: a case report and a review of the literature

  • Lee, Sang Min;Leem, Dae Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.47 no.2
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    • pp.135-140
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    • 2021
  • Since the first report of a nasal septal abscess (NSA) from a dental origin (1920), six articles have been published in the English literature to date. The most common cause of NSA is an infection of the nasal septal hematoma after trauma. This is a report of an uncommon cause of NSA with a dental origin. A PubMed search performed regardless of year and country using the terms ("nasal septal abscess") OR ("nasal septum abscess") initially yielded 229 articles. After screening, seven articles (eight patients) were selected. Addition of two related articles produced a total of nine articles (10 patients) to be included. The age of the included patients ranged from 7 to 69 years (mean, 32.82 years; standard deviation, ±23.86 years). The sex composition was as followed: males (n=7; 63.6%), females (n=4; 36.4%). Dental histories were various: periapical lesions, caries, extraction, endodontic therapy, and cystic lesions. The maxillary incisor dominated as the tooth of origin. Early diagnosis and treatment of NSAs are important to avoid not only facial deformity, but also severe complications (e.g., intracranial infection). If NSA is suspected in patients without facial trauma, the possibility of a dental origin, especially from the maxillary incisor area, should be considered.

Analysis of the Growth Characteristics of Cardiac Cells According to Mechanical Properties of Substrates Using the Simplified Measurement Technique of Tracker

  • Abdullah, Abdullah;Kanade, Pooja P.;Oyunbaatar, Nomin-Erdene;Jeong, Yun-Jin;Kim, Dong-Su;Lee, Dong-Weon
    • Journal of Sensor Science and Technology
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    • v.31 no.1
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    • pp.6-11
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    • 2022
  • To date, various techniques have been utilized to assess the contractility of cardiomyocytes and their response to drug-induced toxicity. However, these techniques are either invasive or involve complex fabrication methods and expertise. Here, we introduce the use of video-based analysis software to track the motion of cardiomyocytes and assess their contractility. The software, called "Tracker", is freely available and this is the first attempt at using it for cardiac contractility measurement. We used the software to measure the contractile properties of cells cultured on a rigid substrate and two flexible polydimethylsiloxane (PDMS) substrates having different elastic moduli day-wise up to eight days. Contractility was found to be highest in the most flexible substrate. Subsequently, the cardiotoxicity response of the cells on three different substrates was analyzed with verapamil. It was observed that the cells on rigid substrate were primarily affected by drug-induced toxicity, while the drug had a lesser impact on cells on the more flexible PDMS substrate. Evidently, the flexible substrate aided the maturation of cells and had lower drug toxicity, while the cells on PS could not fully mature. The assessment of cardiomyocytes using "Tracker" proved to be simple and reliable.

A Test Method of Drought Resistance of Rice Varieties Using Sloped-Ridges (경사휴에 의한 벼품종의 내한발검정법)

  • Park, K.B.;Lee, S.K.;Park, R.K.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.2
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    • pp.114-117
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    • 1984
  • To investigate a possibility of screening for the drought resistance of rice varieties in the slope-ridges, this experiment was conducted in Youngnam Crop Experiment Station in 1982. Seeds were vertically seeded on the slope-ridges which has 30 degree slopes from water level at the front to 1 meter height at the back side. A significant positive correlation was observed between the height of slope-ridges and the PF values (Y=2.25 +0.007 X, r =0.990${\ast}\;{\ast}$).The heading date delayed, the culm length was shortened and the yield was decreased in accordance with the height of slope-ridges. Based upon the above results, it was possible to read the drought resistance of the rice varieties tested. Samnambyeo, Milyang 26, Jinjubyeo and Nongrimna 1 were classified as the resistant ones, while Knob 361-1-8-6-149 was susceptible one.

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Screening for Soybean Pod Borer(Grapholitha glycinivorella Matsumura) Resistant Lines in Korean. Native Soybean Collections (재래종 대두의 콩나방 피해율에 관한 연구)

  • Kwon, S.H.;Ryu, J.;Kim, J.R.;Chung, K.H.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.2
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    • pp.93-97
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    • 1977
  • The rate of damaged soybean seeds by pod borer averaged 7.4% with the range of 1.2%-21.3% and tended to be higher in the lines with hairy pods comparing to those with hairless pods. The susceptibility of pod borer was negatively correlated with the pod setting date. while was not significantly affected by the weather conditions during the pod setting period.

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A report of 21 unrecorded bacterial species of Korea belonging to the phylum Bacteroidota isolated in 2021

  • Chang-Jun Cha;Che Ok Jeon;Kiseong Joh;Wonyong Kim;Seung Bum Kim;Myung Kyum Kim;Jung-Hoon Yoon
    • Journal of Species Research
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    • v.12 no.spc2
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    • pp.23-32
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    • 2023
  • During screening for indigenous prokaryotic species in Republic of Korea in 2021, a total of 21 bacterial strains assigned to the phylum Bacteroidota were isolated from a variety of environmental habitats including pine cone, seaweed, soil, sea sediment, brackish water and moss. Based on the 16S rRNA gene sequence similarity value of more than 98.7% and formation of a robust phylogenetic clade with the type strain of the closest bacterial species, it was found that the 21 strains belong to independent and recognized bacterial species. There has been no official report that the identified 21 species have been isolated in Republic of Korea up to date. Therefore, 16 species in six genera of two families in the order Flavobacteriales, two species in two genera of two families in the order Cytophagales, one species in one genus of one family in the order Chitinophagales and two species in one genus of one family in the order Sphingobacteriales are proposed as unrecorded species of the phylum Bacteroidota isolated in Republic of Korea. Their Gram reaction, colony and cell morphology, basic phenotypic characteristics, isolation source, taxonomic status, strain ID and other information are described in the species descriptions.