• Journal of Digital Convergence
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• v.12 no.11
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• pp.407-414
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• 2014

The aim of the present study was to carry out quantitative analysis of spatial resolution for the influence of the focus size and digital image post-processing on the Computed Radiography (CR). The modulation transfer functions of an edge measuring method (MTF) was used for the evaluation of the spatial resolution. The focus size of X-ray tube was used the small focus (0.6 mm) and the large focus (1.2 mm). We evaluated the 50% and 10% of MTF for the enhancement of edge and contrast by using multi-scale image contrast amplification (MUSICA) in digital image post-processing. As a results, the edge enhancement than the contrast enhancement were significantly higher the spatial resolution of MTF 50% in all focus. Also the spatial resolution of the obtained images in a large focus were improved by digital image processing. In conclusion, the results of this study should serve as a basic data for obtain the high resolution clinical images, such as skeletal and chest images on the CR.

• Mycobiology
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• v.30 no.2
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• pp.82-87
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• 2002

Species of Phellinus were known to harmful fungi causing white pocket rot and severe plant disease such as canker or heartrot in living trees in the West, but some species have been used to traditional medicines in the Orient for a long time. In this study the partial D1-D2 nucleotide sequences of 28S ribosomal DNA from 13 Phellinus strains were determined and compared with the sequences of 21 strains obtained from GenBank database. According to the neighbor-joining(NJ) method comparing the sequence data the phylogenetic tree was constructed. The phylogenetic tree displayed the presence of four groups. Group I includes P. ferreus, P. gilvus and P. johnsonianus, Group II contains P. laevigatus, P. conchatus and P. tremulae, Group III possesses P. linteus, P. weirianus, P. baumii, P. rhabarbarinus and P. igniarius, and Group IV comprises P. pini, P. chrysoloma. P. linteus and P. baumii, which were used mainly in traditional medicine, belong to the same group, but exactly speaking both were split into two different subgroups. To detect P. linteus only, we developed the PCR primer, D12HR. The primer showed the specific amplification of P linteus, which is permitted to medicinal mushroom in the East. The results make a potential to be incorporated in a PCR identification system that could be used for the rapid identification of this species from its related species, P. linteus especially.

• Molecules and Cells
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• v.20 no.3
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• pp.416-422
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• 2005

We previously used Southern blot analysis to detect restriction-length polymorphisms between male fertile and cytoplasmic male sterile (CMS) cytoplasms at the coxII and atp6 loci of the mtDNA of Capsicum annuum L. Two copies of atp6 were found in each male fertile and CMS pepper lines. Interestingly, one of the copies of atp6 in CMS pepper was a 3'-truncated pseudogene. The open reading frame of the coxII gene was the same in the fertile (N-) and CMS (S-) lines. However, the nucleotide sequence in the S-cytoplasm diverged from that in the N-cytoplasm 41 bp downstream of the stop codon. To develop CMS-specific sequence-characterized amplified region (SCAR) markers, inverse PCR was performed to characterize the nucleotide sequences of the 5' and 3' flanking regions of mitochondrial atp6 and coxII from the cytoplasms of male fertile (N-) and CMS (S-) pepper plants. Based on these data, two CMS-specific SCAR markers, 607 and 708 bp long, were developed to distinguish N-cytoplasm from S-cytoplasm by PCR. The CMS-specific PCR bands were verified for 20 cultivars containing either N- or S-cytoplasm. PCR amplification of CMS-specific mitochondrial nucleotide sequences will allow quick and reliable identification of the cytoplasmic types of individual plants at the seedling stage, and assessment of the purity of $F_1$ seed lots. The strategy used in this report for identifying CMS-specific markers could be adopted for many other crops where CMS is used for F1 seed production.

• Water for future
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• v.28 no.6
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• pp.183-191
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• 1995

Applying microwave, a velocity measurement system has been developed in order to measure the velocity of stream water surface. It's main purpose is the measurement for high velocity of flood water. It is under the developing stage of experimental measurement system. The microwave surface velocity meter uses Doppler effects of microwave. It consists of a radio frequency(RF) part and that of signal processing. Thr RF part has the function of microwave oscillation, reception of reflected wave, and determination of Doppler frequency, etc. Signal processing designates amplification, fast Fourier transform, etx. Various measuring experiments were performed at bridges and a spillway of Taechong re-regulation dam with the microwave velocity meter. Verification test was also made through water tank of ship model test at Research Institute of Ships and Ocean Engineering. It shows 4% error inherent in A/D converter and additional several percentage errors from measurement circumstance. The measuring ranges are from 0.5 to 3.5 m/s. The result shows good linear relationship between carriage velocity and measured velocity, thus proves usefulness as a measuring instrument for flood water velocity. The instrument requires overall re-engineering procedure and number of data should be accumulated and analyzed to treat wind effects and random fluctuations of water surface.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1841-1847
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• 2008

Vibrio vulnificus is a causative agent of serious diseases in humans, resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for the V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1 g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium, using the rpoS gene in pure cultures and in infected oyster tissues.

• Nuclear Engineering and Technology
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• v.37 no.6
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• pp.595-604
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• 2005

A new instrument, an average BDFT (Birectional Flow Tube), was proposed to measure the flow rate in single and two phase flows. Its working principle is similar to that of the Pilot tube, wherein the dynamic pressure is measured. In an average BDFT, the pressure measured at the front of the flow tube is equal to the total pressure, while that measured at the rear tube is slightly less than the static pressure of the flow field due to the suction effect downstream. The proposed instrument was tested in air/water vertical and horizontal test sections with an inner diameter of 0.08m. The tests were performed primarily in single phase water and air flow conditions to obtain the amplification factor(k) of the flow tube in the vertical and horizontal test sections. Tests were also performed in air/water vertical two phase flow conditions in which the flow regimes were bubbly, slug, and churn turbulent flows. In order to calculate the phasic mass flow rates from the measured differential pressure, the Chexal drift-flux correlation and a momentum exchange factor between the two phases were introduced. The test results show that the proposed instrument with a combination of the measured void fraction, Chexal drift-flux correlation, and Bosio & Malnes' momentum exchange model could predict the phasic mass flow rates within a $15\%$ error. A new momentum exchange model was also proposed from the present data and its implementation provides a $5\%$ improvement to the measured mass flow rate when compared to that with the Bosio & Malnes' model.

• The Korean Journal of Physiology
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• v.29 no.1
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• pp.1-11
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• 1995

Alveolar macrophages play a pivotal role in the pathogenesis of silicosis since the macrophages may release a wide variety of toxic and inflammatory mediators as well as mitogenic growth factors. In the present study, the effects of in vitro exposure to silica on release of various mediator such as reactive oxygen species, platelet activating factor(PAF), and interleukin-1 (IL-1) by alveolar macrophages were examined. First, hydrogen peroxide release from alveolar macrophages was monitored by measuring the change in fluorescence of scopoletin in the absence or presence of graded concentration of silica. Significantly enhanced release of hydrogen peroxide was observed at 0.5 mg/ml and above. A maximal enhancement of 10 fold above control was observed at 5 mg/ml silica. Similarly, in vitro exposure to silica also significantly stimulated the generation of chemiluminescence from alveolar macrophages at 0.5 mg/ml and above with n maximal enhancement of 8 fold at 5 mg/ml silica. Second, PAF release from alveolar macrophages after 30 min incubation at $37^{\circ}C$ in absence or presence of zymosan and silica was determined by measuring $^{3}H-serotonin$ release ability of the conditioned macrophage supernates from platelets. 5 mg/ml zymosan as a positive control fur the PAF assay increased PAF release by 19 % of total serotonin release. Furthermore, silica also resulted in significant enhancement of the PAF release compared with that in unstimulated (control) cells, i.e., $17.7{\pm}5.8%$ and $24.0{\pm}4.9%$ of total serotonin release at 5 mg/ml and 10 mg/ml silica, respectively, which represents the release of nanomole levels of PAF. Lastly, IL-1 production by alveolar macrophages was analysed following their stimulation with lipopolysaccharide (LPS) and silica by their capacity to stimulate thymocyte proliferation. $10\;{\mu}g/ml$ LPS resulted in an 11 fold increase in IL-1 production. In comparison, $50\;{\mu}g/ml$ silica resulted in a 4 fold increase in IL-1 release. These data indicate that in vitro exposure of alveolar macrophages to silica activates the release of various bioactive mediators such as reactive oxygen species, PAF and IL-1 which thus contribute to amplification of inflammatory reactions and regulation of fibrotic responses by the lung after inhalation of silica.

• International Journal of Fluid Machinery and Systems
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• v.3 no.4
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• pp.315-323
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• 2010

This work investigates the influence of water compressibility on pressure pulsations induced by rotor-stator interaction (RSI) in hydraulic machinery, using the commercial CFD solver ANSYS-CFX. A pipe flow example with harmonic velocity excitation at the inlet plane is simulated using different grid densities and time step sizes. Results are compared with a validated code for hydraulic networks (SIMSEN). Subsequently, the solution procedure is applied to a simplified 2.5-dimensional pump-turbine configuration in prototype with different speeds of sound as well as in model scale with an adapted speed of sound. Pressure fluctuations are compared with numerical and experimental data based on prototype scale. The good agreement indicates that the scaling of acoustic effects with an adapted speed of sound works well. With respect to pressure fluctuation amplitudes along the centerline of runner channels, incompressible solutions exhibit a linear decrease while compressible solutions exhibit sinusoidal distributions with maximum values at half the channel length, coinciding with analytical solutions of one-dimensional acoustics. Furthermore, in compressible simulation the amplification of pressure fluctuations is observed from the inlet of stay vane channels to the spiral case wall. Finally, the procedure is applied to a three-dimensional pump configuration in model scale with adapted speed of sound. Normalized Pressure fluctuations are compared with results from prototype measurements. Compared to incompressible computations, compressible simulations provide similar pressure fluctuations in vaneless space, but pressure fluctuations in spiral case and penstock may be much higher.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.3
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• pp.205-214
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• 2011

Finite element grid refinements with different intensities having 14 K, 52 K and 211 K on the Yellow Sea (YS) have been constructed to make precise tidal simulations. In the meanwhile 57 K grid was made to the extended North Western Pacific (NWP) sea. Numerical simulation were done based on 32 parallel processors by using pADCIRC v 49.21 model. In the YS tidal simulation on YS-G52K and YS-G211K grid structure, KorBathy30s and ETOPO1 bathymetry data are used and 4 major tidal constituents are prescribed from FES2004. Computed results are in good agreement within 0.138 meter in RMS error for amplification and 14.80 degree of phase compared to observed tidal records. Similar error bounds are acquired in the extended NWP tidal simulation on NWP-G57K grid with 8 tidal constituent prescription on the open boundary.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.4
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• pp.668-675
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• 2001

Recently, the performance of the commercial PCS(Personal Communication Service) system has been improved to the uppermost limit and ultimately the next generation mobile communication is to be realized by IMT-2000 (International Mobile Communication-2000) to provide multimedia services. Therefore, the new type receiving system is researched actively and one of the most important part in a receiver is direct conversion method. The direct conversion method is suitable for low power consumption, small size, MMIC, and low price, which is to be adopted to the next generation mobile communication systems. In this case, however, several problems occur due to DC-offset. The DC-offset suppresses amplification of the required signal because of the leakage signal of frequency synthesizer in the system. In this thesis, the removing method of DC-offset was considered. There are four removing techniques of DC-offset, which are AC-coupling, large capacitor, DC-feedback loop, and DC-free coding. Among these, the AC-coupling method is the most simplest method and the DC-feedback loop method has the best performance. Then, the performance of the AC-coupling method and DC-feedback loop method are evaluated by HP's ADS simulation tool. As a result, the AC-coupling method cannot be used to the digital communication systems due to data loss. On the other hand, it was confirmed that the DC-feedback loop method is suitable for the direct conversion receiver.