• 제목/요약/키워드: Darkfield microscopy

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암시야 현미경 영상에서 혈액 내 박테리아 검출 방법 (Detection of Bacteria in Blood in Darkfield Microscopy Image)

  • 박현준
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2021년도 추계학술대회
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    • pp.183-185
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    • 2021
  • 혈액에서 박테리아를 검출하는 것은 의학 및 컴퓨터비전 영역에서 중요한 연구가 될 수 있다. 본 논문에서는 캐글에서 획득한 366장의 암시야 현미경 영상에서 혈액 내 박테리아를 검출하는 방법을 제안한다. 영상처리 기법을 이용한 전처리작업 및 데이터 증강을 통해 학습 데이터세트를 구축하고, 이를 학습하기 위한 딥러닝 모델을 정의한다. 실험 결과 제안된 딥러닝 모델은 암시야 현미경 영상에서 적혈구와 박테리아를 효과적으로 검출하는 것을 확인하였다. 본 논문에서는 비교적 간단한 모델을 사용하여 학습하였으나, 보다 깊은 모델을 활용하면 더 정확한 결과를 얻을 수 있을 것으로 보인다.

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한국 성인형 치주염 환자의 치주낭 내 세균분포에 관한 암시야현미경적 연구 (Darkfield microscopic study of the bacterial morphotypes in the periodontal pockets of Korean adult periodontitis patients)

  • 박정민;남기윤;이인경;엄흥식;장범석
    • Journal of Periodontal and Implant Science
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    • 제33권2호
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    • pp.247-257
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    • 2003
  • The purpose of this study was to evaluate the difference of subgingival bacterial compositions between periodontally healthy and diseased sites. Subgingival plaque samples were obtained from 100 sites in 20 untreated adult periodontitis patients(experimental group), and 100 sites in healthy individuals(contro1 group). Before sampling, probing pocket depth(PPD) and clinical level of attachment(CAL), Plaque Index(PI), and Sulcus Bleeding Index(SBI) were recorded for each sampled sites. Microbial samples were collected from the bases of gingival sulci or periodontal pockets with sterile curettes. The samples were examined under darkfield microscope(${\times}$400). At least 150 bacteria were evaluated and categorized on the basis of bacterial morphology and motility, i.e. cocci, non-motile rods, motile rods, and spirochetes. In control group, subgingival microbial flora consisted of 73.7% of cocci, 20.0% of non-motile rods, 4.3% of motile rods, and 2.0% of spirochetes. The microbial samples from experimental group consisted of 51.5% of cocci, 19.4% of non-motile rods, 17.6% of motile rods, and 11.6% of spirochetes. The proportion of cocci was higher in control group than in experimental group. Proportions of motile rods and spirochetes were higher in experimental group than in control group. The proportion of nonmotile rods in experimental group and control group was not significantly different. Sulcus Bleeding Index and Plaque Index showed high correlation with the bacterial composition. These findings suggests that examination of subgingival bacterial proportion may serve as more sensitive mirror of the local periodontal status than clinical parameters.