• Molecules and Cells
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• 제40권10호
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• pp.773-786
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• 2017

The loss of green coloration via chlorophyll (Chl) degradation typically occurs during leaf senescence. To date, many Chl catabolic enzymes have been identified and shown to interact with light harvesting complex II to form a Chl degradation complex in senescing chloroplasts; this complex might metabolically channel phototoxic Chl catabolic intermediates to prevent oxidative damage to cells. The Chl catabolic enzyme 7-hydroxymethyl Chl a reductase (HCAR) converts 7-hydroxymethyl Chl a (7-HMC a) to Chl a. The rice (Oryza sativa) genome contains a single HCAR homolog (OsHCAR), but its exact role remains unknown. Here, we show that an oshcar knockout mutant exhibits persistent green leaves during both dark-induced and natural senescence, and accumulates 7-HMC a and pheophorbide a (Pheo a) in green leaf blades. Interestingly, both rice and Arabidopsis hcar mutants exhibit severe cell death at the vegetative stage; this cell death largely occurs in a light intensity-dependent manner. In addition, 7-HMC a treatment led to the generation of singlet oxygen ($^1O_2$) in Arabidopsis and rice protoplasts in the light. Under herbicide-induced oxidative stress conditions, leaf necrosis was more severe in hcar plants than in wild type, and HCAR-overexpressing plants were more tolerant to reactive oxygen species than wild type. Therefore, in addition to functioning in the conversion of 7-HMC a to Chl a in senescent leaves, HCAR may play a critical role in protecting plants from high light-induced damage by preventing the accumulation of 7-HMC a and Pheo a in developing and mature leaves at the vegetative stage.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.353-365
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• 2011

The present study investigates the possible regulatory role of exogenous nitric oxide (NO) in antioxidant defense and methylglyoxal (MG) detoxification systems of wheat seedlings exposed to salt stress (150 and 300 mM NaCl, 4 days). Seedlings were pre-treated for 24 h with 1 mM sodium nitroprusside, a NO donor, and then subjected to salt stress. The ascorbate (AsA) content decreased significantly with increased salt stress. The amount of reduced glutathione (GSH) and glutathione disulfide (GSSG) and the GSH/GSSG ratio increased with an increase in the level of salt stress. The glutathione S-transferase (GST) activity increased significantly with severe salt stress (300 mM). The ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT) and glutathione peroxidase (GPX) activities did not show significant changes in response to salt stress. The glutathione reductase (GR), glyoxalase I (Gly I), and glyoxalase II (Gly II) activities decreased upon the imposition of salt stress, especially at 300 mM NaCl, with a concomitant increase in the $H_2O_2$ and lipid peroxidation levels. Exogenous NO pretreatment of the seedlings had little influence on the nonenzymatic and enzymatic components compared to the seedlings of the untreated control. Further investigation revealed that NO pre-treatment had a synergistic effect; that is, the pre-treatment increased the AsA and GSH content and the GSH/GSSG ratio, as well as the activities of MDHAR, DHAR, GR, GST, GPX, Gly I, and Gly II in most of the seedlings subjected to salt stress. These results suggest that the exogenous application of NO rendered the plants more tolerant to salinity-induced oxidative damage by enhancing their antioxidant defense and MG detoxification systems.

• 한국결정성장학회지
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• 제9권5호
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• pp.527-533
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• 1999

치아용 대체재료로 사용되고 있는 세 가지의 세라믹스, 장석질 자기, 운모를 함유한 유리-세라믹 및 유리침윤 알루미나에 대한 접촉피로을 실제 치아의 접촉상황과 유사한 구형입자를 이용한 헤르지안 압입시험법으로 물에서 수행하였으며, 각 재료에서의 접촉손상이 강도에 미치는 영향을 고찰하였다. 초기의 손상형태는 각 재료가 갖는 미세구조에 의존하여 나타났으며, 장석질 자기는 취성거동을 나타내는 cone 형태의 균열이, 운모를 함유한 유리-세라믹은 준-소성 변형 거동을 나타내는 변형이, 그리고 유리침윤 알루미나는 두 재료의 중간거동을 나타내었다. 그러나 반복하중의 수(n=1~n=$10^6$)가 증가됨에 따라 모든 재료에서 급격한 강도저하를 나타내었으며, 파괴는 접촉피로에 의해 형성된 손상에서 일어났다. 일정하중(200N, 500N 및 1000N)에서 반복하중의 수가 증가됨에 따라 두 번의 강도저하가 일어났으며, 첫 번째의 강도저하는 cone 형태의 균열이 주 요인으로 작용되었으며, 두 번째 강도저하는 반복하중에 따른 radial 형태의 균열에 의해 일어났다. 이러한 radial 형태의 균열발생은 각 재료에서 급격한 강도저하를 가져왔으며, 계속적인 반복하중으로 재료의 파괴를 유발시켰다. 반복하중의 수를 고정시킨 수 압입하중의 변화에 따른 강도저하에 대한 고찰을 통해 장석질 자기가 접촉피로에 대한 손상내구성을 갖음을 알 수 있었다.

• Current Research on Agriculture and Life Sciences
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• 제11권
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• pp.25-30
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• 1993

Hayward/Bruno 나무의 눈은 $-7^{\circ}C$에서 피해를 입었으나 -15와 $-19^{\circ}C$의 저온에서는 심한 피해를 나타내었다. 표피조직은 눈보다 정노에 더 예민하였다. 그리고 저온에 처리후 화분에 재식된 Kiwifruit tree는 $-7^{\circ}C$, $-11^{\circ}C$와 $-15^{\circ}C$의 처리에서는 생존하였고 눈의 싹이 터졌다. 그러나 $-19^{\circ}C$에서 처리한 나무는 접수에서는 전연 싹이 터지 않았고, 대목에서 새로운 가지가 발생되었다. 저온에 의한 Kiwifruit tree의 반응으로는 전개된 잎의 표면에 황색의 반점이 나타났고, 또 새로 발생한 가지가 신장 생장이 되지 않았다. 그래서 앞으로 이러한 저온에 대한 피해를 막기 위하여 저온에 강한 대목의 선발이 필요하다고 믿는다.

• 대한기계학회논문집A
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• 제26권2호
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• pp.365-373
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• 2002

For gas turbine engines, the safe life methodology has historically been used fur fatigue life management of failure critical engine components. The safe retirement limit is necessarily determined by a conservative life evaluation procedure, thereby many components which have a long residual life are discarded. The objective of this study is to introduce the damage tolerant design concept into the life management for aircraft engine component instead of conservative fatigue life methodology which has been used for both design and maintenance. Crack growth data were collected on a nickel base superalloy which have been subjected to combined static and cyclic loading at elevated temperatures. Stress analysis fur turbine disk was carried out. The program for computing creep-fatigue crack growth was developed. The residual lifes of turbine disk component under various temperatures and conditions using creep-fatigue crack growth data were estimated. As the result of analysis, it was confirmed that retirement fur cause concept was applicable to the evaluation of residual life of retired turbine disk which had been designed based on the conventional fatigue life methodology.

• 한국농림기상학회지
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• 제4권4호
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• pp.237-247
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• 2002

Air pollution by acid pollutants is problematic in the whole world. Water acidification has already been deteriorating the forest ecosystem. This study was conducted to analyze the acidity and chemical composition of the open precipitation and throughfall at forests with various geographic locations in Korea. The results of this study are as follows; The open precipitation pH was lowest in Seochun. The throughfall pH showed some buffering capacity in only Quercus mongolica stands. In Pinus rigida(Shingal and Seochun) stands, there was little difference from the open precipitation. Chemical composition of the open precipitation for each sampling site showed that $Ca^{2+}$, N $H_{4}$$^{+}$ and S $O_{4}$$^{2-}$ concentrations had higher value than other ions, and except these ions, the small quantity of ions showed different properties to each site. Changes of ion concentrations in the throughfall showed a tendency to increase. ion concentrations of the throughfall increased with washout and nutrient leaching from the trees. In conclusion, the influence was extended to the pure zone, and the frequency of acid rain is increasing. But, if the deposition of pollutants exceeds the capacity of purification, it would damage forest ecosystem. Further investigations are necessary to identify tolerant tree species to acid pollutants.nts.

• Smart Structures and Systems
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• 제13권1호
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• pp.55-80
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• 2014

The present work pays emphasis on investigating the effect of different types of debonding on the bending behaviour of active sandwich beam, consisting of both extension and shear actuators. An active sandwich beam finite element is formulated by using Timoshenko's beam theory, characterized by first order shear deformation for the core and Euler-Bernoulli's beam theory for the top and bottom faces. The problem of debondings of extension actuator and face are dealt with by employing four-region model for inner debonding and three-region model for the edge debonding respectively. Displacement based continuity conditions are enforced at the interfaces of different regions using penalty method. Firstly, piezoelectric actuation of healthy sandwich beam is assessed through deflection analysis. Then the effect of actuators' debondings with different boundary conditions on bending behavior is computationally evaluated and experimentally clamped-free case is validated. The results generated will be useful to address the damage tolerant design procedures for smart sandwich beam structures with structural control and health monitoring applications.

• Smart Structures and Systems
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• 제4권2호
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• pp.195-208
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• 2008

This paper summarizes the experimental vibration-based structural health monitoring study on a historical monument in Jordan. In this work, and within the framework of the European Commission funded project "wide-Range Non-Intrusive Devices Toward Conservation of Historical Monuments in the Mediterranean Area", a seven and a half century old minaret located in Ajloun (73 km north of the capital Amman) is studied. Because of their cultural value, touristic importance and the desire to preserve them for the future, only non-destructive tests were allowed for the experimental investigation of such heritage structures. Therefore, after dimensional measurements and determination of the current state of damage in the selected monument, ambient vibration tests are conducted to measure the accelerations at strategic locations of the system. Output-only modal identification technique is applied to extract the modal parameters such as natural frequencies and mode shapes. A Non-linear version of SAP 2000 computer program is used to develop a three-dimensional finite element model of the minaret. The developed numerical model is then updated according to the modal parameters obtained experimentally by the ambient-vibration test-results and the measured characteristics of old stone and deteriorated mortar. Moreover, a parametric identification method using the N4Sid state space model is employed to model the dynamic behavior of the minaret and to build up a robust, immune and noise tolerant model.

• Structural Engineering and Mechanics
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• 제72권1호
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• pp.31-41
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• 2019

Structural integrity assessment of piping components is of paramount important for remaining life prediction, residual strength evaluation and for in-service inspection planning. For accurate prediction of these, a reliable fracture parameter is essential. One of the fracture parameters is stress intensity factor (SIF), which is generally preferred for high strength materials, can be evaluated by using linear elastic fracture mechanics principles. To employ available analytical and numerical procedures for fracture analysis of piping components, it takes considerable amount of time and effort. In view of this, an alternative approach to analytical and finite element analysis, a model based on relevance vector machine (RVM) is developed to predict SIF of part through crack of a piping component under fatigue loading. RVM is based on probabilistic approach and regression and it is established based on Bayesian formulation of a linear model with an appropriate prior that results in a sparse representation. Model for SIF prediction is developed by using MATLAB software wherein 70% of the data has been used for the development of RVM model and rest of the data is used for validation. The predicted SIF is found to be in good agreement with the corresponding analytical solution, and can be used for damage tolerant analysis of structural components.

• Structural Engineering and Mechanics
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• 제35권3호
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• pp.283-299
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• 2010

This paper addresses the numerical simulation of fatigue crack growth in arbitrary 2D geometries under constant amplitude loading by the using a new finite element software. The purpose of this software is on the determination of 2D crack paths and surfaces as well as on the evaluation of components Lifetimes as a part of the damage tolerant assessment. Throughout the simulation of fatigue crack propagation an automatic adaptive mesh is carried out in the vicinity of the crack front nodes and in the elements which represent the higher stresses distribution. The fatigue crack direction and the corresponding stress-intensity factors are estimated at each small crack increment by employing the displacement extrapolation technique under facilitation of singular crack tip elements. The propagation is modeled by successive linear extensions, which are determined by the stress intensity factors under linear elastic fracture mechanics (LEFM) assumption. The stress intensity factors range history must be recorded along the small crack increments. Upon completion of the stress intensity factors range history recording, fatigue crack propagation life of the examined specimen is predicted. A consistent transfer algorithm and a crack relaxation method are proposed and implemented for this purpose. Verification of the predicted fatigue life is validated with relevant experimental data and numerical results obtained by other researchers. The comparisons show that the program is capable of demonstrating the fatigue life prediction results as well as the fatigue crack path satisfactorily.