• Korean Journal of Head & Neck Oncology
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• v.22 no.1
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• pp.8-14
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• 2006

Objectives: The p53 tumor suppressor gene has a key role in cellular control mechanisms involving apoptosis and DNA repair, leading to the G1 arrest following DNA damage. Its mutation is one of the most frequent alterations in human cancers. Ki-67 is identified in replicating cells of both benign and malignant lesions, so it can be the predictor of proliferative activity. The aim of this study is to evaluate the expression of p53 and Ki-67 in salivary gland tumors. Materials and Methods: Immunohistochemical analysis was used to detect expression of p53 and Ki-67 in paraffin-embedded samples from 31 benign and 27 malignant salivary gland tumors. Results were analyzed between benign and malignant tumors and compared with the clinical parameters such as stage and recurrence in malignant tumors. Results: p53 overexpression was detected in 19.6% of benign tumors and 40.7% of malignant tumors, but there was no statistical significance. p53 was significantly expressed in Warthin's tumor(45.5%) compared with pleomorphic adenoma(5.9%). Only 5.9% of pleomorphic adenoma were positive for p53, while 60% of carcinoma ex pleomorphic adenoma were positive for p53. Ki-67 was expressed in 3.2% of benign tumors and 51.9% of malignant tumors, which showed significant higher expression in malignant tumors. In malignant tumors, p53 and Ki-67 expressions bore no correlation to stage and recurrence. Conclusion: p53 overexpression is not associated with the progression of malignant tumors, and Ki-67 overexpression can be used as biologic indicator of malignant salivary gland tumors.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.6
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• pp.637-644
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• 2012

In a nuclear power plant, fretting wear due to impact motion between U-tubes and support structures located in steam generators can cause serious problems. In order to guarantee the reliability of the steam generator, the damage due to fretting wear should be thoroughly investigated. The purpose of this study is to elucidate the fretting wear mechanism qualitatively and quantitatively. Hence, fretting wear simulation is performed for the environments to which the actual steam generators in nuclear power plants are exposed. Initial experimental results are obtained for various experimental parameters, and the effect of the work rate and temperature on fretting wear is evaluated. In water, the wear coefficients for $90^{\circ}C$, $200^{\circ}C$, and $340^{\circ}C$ are found to be $9.051{\times}10^{-16}\;Pa^{-1}$, $3.009{\times}10^{-15}\;Pa^{-1}$, and $2.235{\times}10^{-15}\;Pa^{-1}$, respectively. It is also found that the wear coefficient at room temperature is larger than that at low temperature in water because of the dynamic viscosity of water.

• The Journal of Engineering Geology
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• v.26 no.1
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• pp.101-115
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• 2016

We carried out laboratory material tests on two cements (KS-1 ordinary Portland and Class G) with changing W/S (Water/Solid) and the content of fly ash in order to evaluate their physical and mechanical properties. The specimens of KS-1 ordinary Portland cement were prepared with varying W/S (Solid=cement) in weight, while those of Class G cement were prepared with changing the content of fly ash in volume but maintaining W/S (Solid=cement+fly ash). The results of the material tests show that as the W/S in KS-1 ordinary Portland cement and the content of fly ash in Class G cement increase, the properties (density, sonic wave velocity, elastic constants, compressive and tensile strengths, thermal conductivity) decrease, but porosity and specific heat increase. In addition, an increase in confining pressure and in the content of fly ash leads to plastic failure behavior of the cements. The laboratory data were then used in a stability analysis of cement sheath for which an analytical solution for computing the stress distribution induced around a cased, cemented well was employed. The analysis was carried out with varying the injection well parameters such as thickness of casing and cement, injection pressure, dip and dip direction of injection well, and depth of injection well. The analysis results show that cement sheath is stable in the cases of relatively lower injection pressures and inclined and horizontal wells. However, in the other cases, it is damaged by mainly tensile failure.

• Journal of Life Science
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• v.26 no.11
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• pp.1308-1312
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• 2016

Heavy metals such as gallium (Ga) cause serious physiological damage to exposed organisms, mostly of aquatic species. Ga one of the inter-metallic, transition elements increasingly being used in making high-speed semiconductors, such as Ga arsenide. The purposes of this study were to investigate the effects of Ga on acute toxicity, serum biochemical changes, and erythrocyte morphological changes in the blood stream of goldfish (Carassius auratus). Median lethal concentrations were determined in acute tests. The 96 hr $LC_{50}$ value was 9.15 mg/ml. Goldfish were exposed to different Ga concentrations (2.0, 4.0, and 8.0 mg/ml) for 30 days to assess its toxic effects. The results indicate that the measured serum biochemistry parameters (including glucose, blood urea nitrogen, creatinine, cholesterol, and triglyceride) of the Ga-exposed fish groups differed significantly from the untreated fish group. In addition, a change in the erythrocytes' morphology at a high concentration (8.0 mg/ml) of Ga exposure shows respiratory problems. Our results suggest that 2.0 mg/ml is proposed as a biologically safe concentration that can be used for establishing tentative water quality criteria concerning the same-size goldfish.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.5
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• pp.1485-1489
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• 2004

Panax ginseng is the one of best famous phytochemical plant in the world and it's various positive effects such as antioxidant, regulation of immunity are very well known. In this study, we investigated primary the cell viability and morphological change and secondary an antioxidative effect and liver function improvement of extract from Ginseng folium and stem in CCl4 intoxicated rats. The NCTC cell line were used for cell viability and sirius red staining before the animal experiment. The female Sprague-Dawley rats (90-100g) were divided into 3 groups (Normal, AC: CCl₄ treated group, GFS: CCl₄+ extract of Ginseng folium and stem treated group) and acute liver damage was developed by one time administration of CCl₄ mixture (0.5㎖/rat). The liver tissue and sera were collected and used for quantitative measurement of enzyme activity (AST, ALT, ALP, BUN), MDA and Hyp. As a result, cell viability in GFS treated group (in concentration of 3.33-33.33㎎ GFS/200㎕ medium) was 180.9-241.0% significantly and dose dependently higher than in control group. And potential state of cell growth and differentiation and no criteria of cytoplasm lysis and nucleus breaking were observed in control and GFS group. The parameters of liver function (AST and ALP) in sera of GFS group showed significantly 93% and 67.6% lower than AC group (p<0.005-0.05). And the level of ALT and BUN showed fast similar in AC group and GFS group. The concentration of MDA in liver was decreased 576.5% significantly in GFS group when compared with AC group (p<0.005). The content of Hyp in GFS group is merely lower than in AC group. In conclusion, the water extract of Ginseng folium and stem such as Ginseng radix may be possessed the antioxidative effect and improvement of liver function in CCl₄ intoxicated rats.

• Journal of Wetlands Research
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• v.21 no.spc
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• pp.90-97
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• 2019

In recent years, flood due to the consecutive storm events have been occurred and property damage and casualties are in increasing trend. This study calls the consecutively occurred storm events as a mega rainfall scenario and the discharge by the scenario is defined as a mega flood discharge. A mega rainfall scenario was created on the assumption that 100-year frequency rainfall events were consecutively occurred in the Gyeongancheon stream basin. The SSARR (Streamflow Synthesis and Reservoir Regulation) model was used to estimate the mega flood discharge using the scenario in the basin. In addition, in order to perform more reasonable runoff analysis, the parameters were estimated using the SCE_UA algorithm. Also, the calibration and verification were performed using the objective functions of the weighted sum of squared of residual(WSSR), which is advantageous for the peak discharge simulation and sum of squared of residual(SSR). As a result, the mega flood discharge due to the continuous occurrence of 100-year frequency rainfall events in the Gyeongan Stream Basin was estimated to be 4,802㎥/s, and the flood discharge due to the 100-year frequency single rainfall event estimated by "the Master Plan for the Gyeongancheon Stream Improvement" (2011) was 3,810㎥/s. Therefore, the mega flood discharge was found to increase about 992㎥/s more than the single flood event. The results of this study can be used as a basic data for Comprehensive Flood Control Plan of the Gyeongan Stream basin.

• Journal of Korea Water Resources Association
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• v.44 no.11
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• pp.875-887
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• 2011

The objective of this study is to develop and evaluate a Korean threshold runoff computation method. The selected study area is the Han-River basin and the stream channels in the study area are divided into 3 parts; natural channel and artificial manmade channel for small mountainous catchments, and main channel for master stream. The threshold runoff criteria for small streams is decided to 0.5 m water level increase from the channel bottom, which is the level that mountain climbers and campers successfully escape from natural flood damage. Threshold runoff values in natural channel of small mountainous area are computed by the results from the regional regression analysis between parameters of basin and stream channel, while those in artificial channel of small mountainous area are obtained from the data of basin and channel characteristics parameter. On the other hand, the threshold runoff values for master channel are used the warning flood level that is useful information for escaping guideline for riverside users. For verification of the threshold runoff computation method proposed in this study, three flash flood cases are selected and compared with observed values, which is obtained from SCS effective rainfall computation. The 1, 3, 6-hour effective rainfall values are greater than the corresponding threshold runoff values represents that the proposed computation results are reasonable.

• Journal of the Korean Geotechnical Society
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• v.18 no.1
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• pp.91-99
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• 2002

In this study, a new methodology for the assessment of liquefaction potential is proposed. Since there is no data on the liquefaction damage in Korea, the dynamic behavior of fully saturated soils is characterized through laboratory dynamic tests. There are two experimental parameters related to the soil liquefaction resistance characteristics : the one is the index of disturbance determined by $G/G_{max}$ curve and the other is a plastic shear strain trajectory evaluated from stress-strain curve. The proposed methodology takes advantage of the site response analysis based on real earthquake records to determine the driving effect of earthquake. In the evaluation of liquefaction resistance characteristics, it is verified experimentally that the magnitude of cyclic shear stress has no influence on the critical value of plastic shear strain trajectory at which the initial liquefaction occurs. Cyclic triaxial tests under the conditions of various cyclic stress ratios and torsional shear tests are carried out far the purpose of verification. Through this study, the critical value at the initial liquefaction is found unique regardless of the cyclic stress ratio. It is also f3und that liquefaction resistance curve drawn with disturbance and plastic shear strain trajectory can simulate the behavior of fully saturated soils under dynamic loads.

• Journal of the Korean earth science society
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• v.34 no.6
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• pp.492-506
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• 2013

The historical accounts and materials about the eruption of Mt. Baekdusan as observed by the geological survey is now showing some signs of waking from a long slumber. As a response of the volcanic eruption of Mt. Baekdusan, water release may occur from the stored water in Lake Cheonjii caldera. The volcanic flood is crucial in that it has huge potential energy that can destruct all kinds of man-made structures and that its velocity can reach up to 100 km $hr^{-1}$ to cover hundreds of kilometers of downstream of Lake Cheonji. The ultimate goal of the study is to estimate the level of damage caused by the volcanic flood of Lake Cheon-Ji caldera. As a preliminary study a scenario-based numerical analysis is performed to build hydrographs as a function of time. The analysis is performed for each scenario (breach, magma uplift, combination of uplift and breach, formation of precipitation etc.) and the parameters to require a model structure is chosen on the basis of the historic records of other volcanos. This study only considers the amount of water at the rim site as a function of time for the estimation whereas the downstream routing process is not considered in this study.

• Nutrition Research and Practice
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• v.9 no.1
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• pp.22-29
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• 2015

BACKGROUND/OBJECTIVES: Recently, anthocyanins have been reported to have various biological activities. Furthermore, anthocyanin-rich purple corn extract (PCE) ameliorated insulin resistance and reduced diabetes-associated mesanginal fibrosis and inflammation, suggesting that it may have benefits for the prevention of diabetes and diabetes complications. In this study, we determined the anthocyanins and non-anthocyanin component of PCE by HPLC-ESI-MS and investigated its anti-diabetic activity and mechanisms using C57BL/KsJ db/db mice. MATERIALS/METHODS: The db/db mice were divided into four groups: diabetic control group (DC), 10 or 50 mg/kg PCE (PCE 10 or PCE 50), or 10 mg/kg pinitol (pinitol 10) and treated with drugs once per day for 8 weeks. During the experiment, body weight and blood glucose levels were measured every week. At the end of treatment, we measured several diabetic parameters. RESULTS: Compared to the DC group, Fasting blood glucose levels were 68% lower in PCE 50 group and 51% lower in the pinitol 10 group. Furthermore, the PCE 50 group showed 2-fold increased C-peptide and adiponectin levels and 20% decreased HbA1c levels, than in the DC group. In pancreatic islets morphology, the PCE- or pinitol-treated mice showed significant prevention of pancreatic ${\beta}$-cell damage and higher insulin content. Microarray analyses results indicating that gene and protein expressions associated with glycolysis and fatty acid metabolism in liver and fat tissues. In addition, purple corn extract increased the phosphorylation of AMP-activated protein kinase (AMPK) and decreased phosphoenolpyruvate carboxykinase (PEPCK), glucose 6-phosphatase (G6pase) genes in liver, and also increased glucose transporter 4 (GLUT4) expressions in skeletal muscle. CONCLUSIONS: Our results suggested that PCE exerted anti-diabetic effects through protection of pancreatic ${\beta}$-cells, increase of insulin secretion and AMPK activation in the liver of C57BL/KsJ db/db mice.