• Title/Summary/Keyword: Dairy

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태아섬유아세포에 UPIII-hEPO 유전자 도입과 핵이식 난자의 체외발달율

  • 박미령;조성근;윤희준;조황윤;임여정;박종주;김진회
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.65-65
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    • 2002
  • 본 연구는 태아 섬유아세포에 hEPO 를 도입하여 transfedtion된 체세포를 공여핵으로 사용하여 복제수정란을 생산함에 있어서 배반포 발달율을 조사하고자 하였다. 실험에 공시된 공여핵은 임신 30일령의 태아로부터 섬유아 세포를 회수하여 10% FBS 첨가된 ES-DMEM 배지에서 3-4일 동안 배양하여 confluent 를 형성시킨 후 0.25% trypsin을 처리하여 neo-EGFP 와 UPⅢ-hEPO 유전자가 1:9로 함유된 배지에서 electroporation을 실시하였다. (중략)

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Follow-Up of Exogenous DNA by Sperm-Mediated Gene Transfer Via Liposome

  • Jo Hwang-Yun;Jo Seong-Geun;Yun Hui-Jun;Park Mi-Ryeong;Im Yeo-Jeong;Park Jong-Ju;Kim Jin-Hoe
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.86-86
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    • 2002
  • To examine the feasibility of using a sperm vector system for gene transfer, we have investigated the binding and the uptaking of foreign DNA into the sperm nucleus by PCR, in situ hybridization and LSC. We have also examined the transportation of exogenous DNA into oocytes by immunofluorescene via PCR. Sperm cells were incubated with DNA/liposome complexes (1:4 ratio) in fertilization medium with BSA or without BSA. (omitted)

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Dairy wastewater treatment using microalgae for potential biodiesel application

  • Choi, Hee-Jeong
    • Environmental Engineering Research
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    • v.21 no.4
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    • pp.393-400
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    • 2016
  • The aim of this study was to evaluate the biomass production and dairy wastewater treatment using Chlorella vulgaris. The results indicated that the maximum percentages of biochemical oxygen demand, chemical oxygen demand, suspended solids, total nitrogen, and total phosphorus removed were 85.61%, 80.62%, 29.10%, 85.47%, and 65.96%, respectively, in dairy effluent at 10 d. A maximum of 1.23 g/L dry biomass was obtained in 7 d. The biomass productivity was strongly influenced by the nutrient reduction in the dairy effluent. The biodiesel produced by the C. vulgaris in the dairy effluent was in good agreement with the American Society of Testing and Materials-D6751 and European Standards 14214 standards. Therefore, using dairy effluent for microalgal cultures could be a useful and practical strategy for an advanced, environmentally friendly treatment process.

Modeling of Farm Size Dairy Feed Mill in Korea

  • Park, K.K.;Kim, T.W.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.565-583
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    • 1993
  • In order to reduce the production cost and improve the quality of dairy feed, several dairy feed mill model suitable for Korean farm size are developed. Also, capital requirement and operating costs of the model mill are analyzed. And these analyzed data are compared with the commercial feed production cost, in order to test whether the model mills are suitable or not in Korean dairy farm. Also optimum model is recommended depending on size of dairy farm. As a result, developed model mill(TMR) is very useful in Korea dairy farm not only reduce production cost up to 78% but also improve the qualities.

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Effect of Rumen Protected Methionine on the Concentration of Milk Taurine in Dairy Cows (반추위 보호 Methionine이 젖소의 유타우린 함량에 미치는 영향)

  • Park, Dong-Jin;Yeo, Young-Keun;Kim, Dong-Shin
    • Journal of Dairy Science and Biotechnology
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    • v.19 no.1
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    • pp.1-3
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    • 2001
  • This study was conducted to increase the content of taurine in milk from the dairy cows fed the rumen protected DL-methionine matrix and methionie hydroxy analog matrix for 42days. Dairy cows fed rumen protected DL-methionine matrix showed 23% increased milk taurine concentration compared to the value of the control groups. Dairy cows fed rumen protected methionine hydroxy analog matrix showed 53% increased milk taurine concentration compared to the value for the control groups (p<0.01).

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Environmental Conditions and Resource Management in Smallholder Dairy Farms in Thailand. I. Production Systems and Management of Resources

  • Skunmun, P.;Boonsom, J.;Kaewsuwan, S.;Chantalakhana, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.2
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    • pp.215-219
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    • 1999
  • This study aims to make detail examination of smallholder dairy farming systems in the Nongpho Dairy Cooperative. Forty-three dairy farms were selected from three geographical areas i.e. irrigated area, municipality area, and factory area. Within each area some number of sample farms were selected from each of the three levels of farm and animal crowdedness (very crowded, crowded, and not crowded farms). Detail data were collected during 1996 to 1997, they were socio-economic conditions of the sample farms and farmers, dairy production systems and management of resources (animals, bam, feeds, stocking rates, herd structure, animal body conditions, milk yield and milk quality, manure and farm wastes management, and other related items). Detail information useful for the improvement of farm production efficiency were discussed. It was very clear that much improvement of smallholder dairy production can be achieved if the recommendations given by this study were implemented.

Project to increase productivity of livestock in Honam area against UR (호남지역의 양축농가에 있어서 UR에 대처한 가축의 생산성 향상에 관한 연구)

  • Lee, Joo-mook;Kwon, Oh-deog;Chae, Joon-seok;Kim, Myeong-chul;Kim, Heung-seob;Lee, Sung-jae;Lee, Hoo-sik;Roh, Soo-il;Kim, Kil-soo
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.195-212
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    • 1994
  • For the purpose to increase productivities of livestock the present investigations were undertaken in order to clarify the clinical and suclinical status of Korean native cattle, dairy cattle(holstein) and Korean native goat. Blood, feces and urine samples were collected from 247 Korean native cattle(222 adult and 25 calf), 224 dairy cattle(211 adult and 13 calf) and 142 Korean native goat rearing at Chonbuk area and analyzed for clinical, serum chemical, hematological and urinary findings. In addition, we were examined the infection rate of theileriosis, internal parasite and ring worm. The mean value for each component was calculated by statistical analysis using Excel computer program. From these investigations the following results were obtained. The mean values for RBC, PCV and etc in 433 adult cow(Korean native cattle and dairy cattle) were similar with other reports. But the mean values for MCHC of all species were lower than normal. Adult Korean native cattle and adult dairy cattle which showed hematologically normal levels were only 9.01% and 9.48%, respectively. Abnormally high values for PCV, RBC and Hb were recorded in 7.66% of adult Korean native cattle, 20% of korean native calf, 15.38% of dairy calf and 13.36% of Korean native goat. Adult Korean native cattle and adult dairy cattle which showed hematologically anemia were 4.95% and 19.43% respectively, but Korean native calf and dairy calf showed normal. Adult Korean native cattle, adult dairy cattle, Korean native calf and dairy calf which showed normal serum protein level 84.0%, 90.8%, 50% and 44.4%, respectively. In present investigations, 50% of Korean native calf and 55.6% of dairy calf were decreased serum protein values under range. These abnormally decreased serum protein values mean the shortage of antibody, and these have a possibility to occurs to pneumonia and diarrhea. From these results, the economical loss caused by pneumonia was calculated as 124,038,833 won in the KNC and 742,703,430 won in the dairy calf rearing in Chonbuk area. Calculated economical loss caused by enteritis was 56,658,690 won in Korean native cattle, 476,775,799 won in dairy calf, and the total loss amount of 533,434,488 won in Chonbuk area. Abnormally high values$(21.7{\pm}4.0mg/dl)$ for serum calcium were recorded 49.6% in dairy cattle. The mean values for serum total cholesterol were $170.8{\pm}99.8mg/dl$ in Korean native cattle, $196.0{\pm}40.6mg/dl$ in Korean native calf, $202.9{\pm}86.0mg/dl$ in adult dairy cattle and $289.4{\pm}97.5mg/dl$ in dairy calf. The infection rate of internal parsite were as follows; adult Korean native cattle: 21.2%, Korean native calf: 8.0%, adult dairy cattle: 67.8% and Korean native goat: 81.5%. The estimated economical loss caused by internal parasites infection were 1,120,855,837 won in Korean native calf, 4,994,959,405 won in adult Korean native cattle, 3,334,751,066 won in adult holstein, and the total loss amount to 9,450,566,308 won. The infection rate of theileriosis were 1.4% in Korean native cattle and 6.6% in dairy cattle. The presumed economical loss by T. sergenti infection were 154,408,482 won in Korean native cattle and 171,577,237 won in dairy cattle rearing at Chonbuk area. The infection rate of ringworm were 0.5% in Korean native cattle, 0.9% adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12,061,532 won in Korean native cattle, 16,895,403 won in dairy cattle, and the total estimated loss amount to 28,955,935 won a year in Chonbuk area.

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Development of Dairy Products using Functional Effects of Natural Spices and Herbs (천연 향신료의 기능성을 이용한 유제품개발)

  • Park, Ki-Moon
    • Journal of Dairy Science and Biotechnology
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    • v.16 no.2
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    • pp.137-153
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    • 1998
  • It is found that many kinds of spices and herbs have a deodorization, acrid, coloration, antimicrobial or antioxidant effect. They also have physiological actions such as antiaging effect, anticancer effect, endurance enhancement, or stress degradation. Therefore, development of the dairy products with those above benefical effects of natural spices and herbs is necessary, and the functional dairy products which has an anticancer, antiobesity or coelenterate will be useful foods, especially patients.

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