• Toxicological Research
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• v.23 no.1
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• pp.11-17
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• 2007

This study was carried out to investigate the effect of LAB (Lactic acid bacteria: Lactobacillus acidophilus, Bifidobacterium bifidum and Streptococcus thermophilus) on detoxication of damaged liver in carbon tetrachloride ($CCl_4$) and ethanol (25%)-treated rats. Rats had been daily (twice a day) pre-treated with saline (0.5 ml/kg: untreated group), $CCl_4$ (0.5 ml/kg: other groups) for 6 days. At seventh day, after treating rat with $CCl_4$ and then, mixture of LAB ($10^{11}$/0.5 ml: LAB group), saline (0.5 ml/kg: untreated group, $CCl_4$ group), and biphenyl dimethyl dicarboxylate (DDB) (50 mg/kg: DDB group) were treated orally with $CCl_4$ for 8 days. Ethanol is treated as the same manner instead of $CCl_4$. To investigate the hepatoprotective effect, rats treated with $CCl_4$ and ethanol were analyzed with serum GOT and GPT level. The GOT and GPT levels of LAB group was lower than the level of $CCl_4$ and DDB group. Especially, compared with data of $CCl_4$ group, GPT activity showed statistically significant result in the significance level of p < 0.05. The LAB group treated with ethanol also showed lower level of GOT and GPT than the other control groups treated with ethanol. The triglyceride level of serum decreased more in a group treated special materials (DDB and LAB group) than ethanol group. As well, the effect of LAB on the antifatigue has been investigated. The animals (10/group) were divided into 4 groups (untreated group, Carrier group, Red-ginseng group, LAB group). Each group was given carrier (0.9 mg/0.2 ml), red ginseng extract (200 mg/kg), and mixture of LAB ($10^{11}$/0.2 ml). Special materials were given for three weeks. After finishing treating through oral, horizontal wire test, rotarod test, and forced swimming test were performed. The time of resistance to fatigue of the group, fed with mixture of LAB, was longer than the time when mice treated with red-ginseng that the effect was already revealed. The result of this study revealed that LAB could decrease hepatocelluar injury compared with rats treated orally with $CCl_4$ and ethanol, and could also decrease fatigue.

• Therapeutic Science for Rehabilitation
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• v.7 no.2
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• pp.51-61
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• 2018

Objective: The purpose of this study was performed to modify the performance-based assessment tool, Melbourne Low-Vision ADL Index (MLVAI) had been developed in Australia to suit the Korea culture to verify the reliability and validity. Methods: The subjects were only over the age of 20 living in communities, 26 with low-vision and 42 normal persons. The Korean MLVAI was completed through the expert translation verification and validation of the configuration tool. The validity of the Korean MLVAI was established through the content, discriminant, and convergent validity. Also, the reliability was analyzed through internal consistency reliability for the items, test-retest, and interrater reliability. Results: The Content Validity Index(CVI) was more than .78. There was a statically significant low in low-vision. Also, there was a statically significant low in low-vision. The convergent validity was determined the correlation coefficient of .751 analyzing LVQOL and Korean MLVAI total score, had a significant correlation(p<.05). Cronbach's ${\alpha}$ coefficient indicated an internal consistency of .983(p<.05). Test-retest reliability had a high, significant correlation of .976 and interrater reliability had a high, an intraclass correlation coefficient of .91(p<.05). Conclusion: The results of this study mean that the Korean MLVAI which was modified to fit the Korean is the ADL assessment tool with both validity and reliability. Based on this study, the Korean MLVAI can be used as a useful ADL assessment for OT interventions in low vision.

• Therapeutic Science for Rehabilitation
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• v.7 no.3
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• pp.23-33
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• 2018

Objective : This systematic review aimed to investigate the effect of focal muscle vibration in patients with post-stroke spastic hemiplegia. Methods : We searched literature published between April 2009 and October 2017 using PubMed and RISS databases. The main search terms were Vibration therapy, Focal vibration, Somatosensory, Upper limb, and Spasticity after stroke. Based on inclusion/exclusion criteria, 6 articles were selected. Results : Articles on focal muscle vibration intervention ranged from evaluation of application-only vibration to muscle vibration with task-oriented activity. Intervention effects on upper extremity spasticity and function and activities of daily living were assessed. There were significant effects on upper extremity spasticity, function, and cortical excitability. Conclusions : This study can provide information on focal muscle vibration for use by clinical therapists. However, further studies are needed to identify the optimal stimulation site and frequency/amplitude of application to maximize the effects of focal muscle vibration.

• Korean Journal of Poultry Science
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• v.43 no.1
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• pp.21-29
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• 2016

A total of 144 one-day-old male broilers (ROSS ${\times}$ Ross 308) ($42{\pm}0.1g$) were used in a completely randomized design and allotted to one of three dietary treatments to give 6 replicates per treatment (8 birds per cage). The three watery dietary treatments were 1) a corn-soybean meal-based relation [Negative control (NC); no antimicrobial compounds added] with tab water, 2) 8 ppm liquid-alpha-lipoic acid [Positive control (PC)] or 3) 100 ppm liquid-resveratrol. All dietary treatments were continuously provided as a liquid form. Birds were housed in a battery cage (n=48), and were offered liquid dietary treatments at all times. The ambient temperature was maintained at $32{\pm}1^{\circ}C$ for the first 3 weeks and decreased gradually to $28{\pm}1^{\circ}C$ by the end of the experiment (d 35) to induce moderate heat stress. One bird per pen (n=6) was euthanized via cervical dislocation at day 21 to obtain terminal ileum to measure villous height and crypt depth, while another bird per pen (n=6) was similarly euthanized at day 35 and used to harvest breast meat and drumsticks to evaluate meat quality traits. Birds fed liquid-resveratrol did not improve (P>0.05) body weight and average daily gain compared with those fed other dietary treatments from day 1 to day 35. Birds fed liquid-resveratrol had no effect (P>0.05) on villous height compared with birds were in other dietary treatments at day 21. Liquid-resveratrol had no effect (P>0.05) on thiobarbituric acid reactive substance (TBARS) in drumsticks compared with other dietary treatments at day 35. Furthermore, birds fed liquid-resveratrol had no effect (P>0.05) on DPPH radical scavenging activity both in breast meat and drumsticks compared with those fed other dietary treatments at day 35. Morphological changes of ileum were not observed by immunofluorescence, and the level of occludin protein from ileum extracts also did not differ among groups. Our results suggested that liquid form of antioxidant compounds used in the current study such as alpha-lipoic acid (8 ppm) or resveratrol (100 ppm) did not improve growth performance, meat quality traits and histology of terminal ileum compared with those fed negative control in broiler chickens under moderate heat stress for 35 days.

• The Journal of Korean Society for School & Community Health Education
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• v.20 no.3
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• pp.113-122
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• 2019

Objectives: This study was conducted to investigate the snack intake and brushing behavior according to the oral health education experience in some schools in Busan, and to investigate whether the oral health education experience affects the snack intake and brushing behavior. Methods: This study surveyed the entire fourth grade of elementary school in two districts by participating in university-linked oral health education activities run under the jurisdiction of the education office business to examine changes in the behavior of elementary school students in their snack intake and toothbrush. The survey was conducted on oral health education in elementary schools, prior oral health education experience before and after the activity, whether or not the brushing classroom was operated, and contents related to eating snacks and brushing behaviors. Result: Among the general characteristics of some schools in Busan, 69.9% of students have experience in oral health education and 30.1% of people have no experience in oral health education. 20.0%, 16.3% were 'normal' and 63.7% were 'helpful'. The brushing behavior according to the oral health education was 44.9% in the number of brushings, 44.9% in the number of brushings, 45.7% in 2-3 minutes in the time of brushing, 41.2% in the brushing method by sweeping the brush up and down. In the daily brushing period, 'after breakfast' was the highest at 72.3%, and the parent's brushing instruction was 'to lead' at 65.1%. The amount of sugar in subjective snacks was the highest with 60.6% of sugar content, and the parents had the highest level of 52.2% for parents' snack intake. This result was more significant than the students without oral health education experience. Conclusion: Based on the results of this study, the number and time of brushing, the method and timing of brushing according to the experience of oral health education. Students who had oral health education experience higher than those who did not have oral health education, but had a lower tendency to brush after lunch at school and before going to bed. For better oral health, the effect of oral health education will be better if the school has more systematic toothbrushing at lunch time and parental guidance at home.

• Journal of Veterinary Clinics
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• v.23 no.3
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• pp.320-324
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• 2006

The purpose of the present study was to compare pulsatile type(Twin Pulse Life Support; TPLS) with rotary type hemodialyzer(AK95) in order to reduce the dialysis time and to improve dialysis effect. Three healthy dogs(about 30 kg BW) were used. Experimental renal failure was induced by the ligation of bilateral renal artery. A pair of catheters were implanted in jugular vein for dialysis. Daily investigated parameters included clinical signs such as vomiting, fecal appearance and activity, and also laboratory data such as PCV, TP, BUN and creatinine. Hemodialysis was started above 90 mg/100 ml BUN level and, laboratory data were measured every an hour for 4 hours. Heparin was administered 300 IU/Kg before dialysis and 150 IU/Kg via IV route every 90 minutes during dialysis. Clinical signs after induction renal failure were shown severe vomiting, anorexia, diarrhea, mucous feces, ataxia, dilated pupil and episcleral hyperemia. The average of BUN value decreased hourly $99{\pm}12.1,\;84{\pm}12.2,\;72{\pm}8.0,\;58{\pm}7.1,\;48{\pm}5.2,\;and\;39{\pm}3.2mg/100ml$ by hemodialysis. The average of creatinine value decreased $7.8{\pm}0.61,\;6.4{\pm}0.40,\;5.3{\pm}0.42,\;4.5{\pm}0.23,\;4.0{\pm}0.41,\;and\;3.4{\pm}0.42mg/100ml$ according to hemodialysis an hour. There are not significantly differences BUN, creatinine, PCV and TP values between pulsatile and rotary type hemodialysis. These results suggested that effects of hemodialysis with Pulsatile type(TPLS) are not significantly difference as compared with hemodialysis of rotary type(AK95). Further research is needed in order to estimate the influence of cardiovascular and pulmonary system in hemodialysis of pulsatile type.

• Journal of Veterinary Clinics
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• v.8 no.2
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• pp.127-142
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• 1991

This study was carried out to investigate fatty liver in Korean black goats. Adult female goats were divided into 3 test groups(A, B and C). Group A and B of goats each received 3 test consecutive daily doses of DL-ethionine at 75mg/kg and 150mg/kg body weight, respectively. Group C of goats was given 3 consecutive doses of the compound every 48 hours at 150mg/kg body weight. The clinical symptoms, hematological values, serum chemical values and histopathological study of the liver were investigated in the test animals. The results obtained are as follows ; 1. Fatty liver were observed in every test animal. 2. Some clinical symptoms( anorexia, depression) were appeared from 1st day to 7th day after administration of the compound in every test animal. In addition to these symptoms, diarrhea and salivation were generally observed in test animals which were given the compound at 150mg/kg body weight. The degree of these symptoms was dose dependent. 3. There was no significant variations in total WBC counts and fibrinogen values in the blood of test goats. The PCV values were significantly increased on 5th day of dosing in group A and B of goats. 4. The total lipid value was not changed but the concentration of NEFA was significantly increased on 3rd day of dosing with the compound and returned to normal value after 10 days hereafter. The value of triglycerides was significantly increased on 1st day and returned to normal value on 3rd day of dosing. The value of cholesterol was significantly decreased on 3rd day and returned to normal value on 10th day after treatment. 5. Total protein level was decreased on 10th day of dosing in the groups of B and C, and billirubin level was significantly increased on 7th day of dosing in every test group and returned to normal level after 13th day of administration. 6. The activity of GGT in serum was not changed while the activities of SDH and AST were significantly increased in every test goat and those values were returned to normal after 10~13th day of trestment. 7 The 35K-protein fraction in serum was not detected by SDS-polyacrylamide gel electrophoresis, but this protein fraction was detected by the same method after treating the 21st and 22nd fraction which were obtained by column chromatography with Sephadex G-100. 8. The affected liver was congested and swollen on 3rd day, and yellowish brown in color and mottled appearance on 7th day of treatment. Histopathologically, fat droplets were common in the hepatocytes, this change was intensive on 7th day after treatment in group B and C. Hepatic cell necrosis was observed in some livers but this pathological change was disappeared and returned to normal after 13 days of treatment.

• Journal of Applied Biological Chemistry
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• v.53 no.1
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• pp.44-50
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• 2010

Tae-Um-Jo-wee-Tang (TUJWT) and Do-Dam-Tang (DDT) have been used as an anti-obesity herbal medicine but their effect and mechanism of action have not been studied. We investigated the effects of TUJWT and DDT on energy and glucose homeostasis using Sprague Dawley female rats with diet-induced obesity. The mechanisms of action of TUJWT and DDT were studied whether they had anti-obesity effects. Rats fed a high-fat diet were divided into 3 groups: rats in each group received 2 g water extracts of modified TUJWT and DDT, or 2 g cellulose per kg body weight (a negative control) on a daily basis. A further group was fed a low-fat diet as a positive control. We found that DDT significantly decreased body weight and body fat (mesenteric fat and retroperitoneal fat) more than the control. This decrease was due to the reduction in energy intake but no changes of energy expenditure. However, DDT increased fat oxidation as a major energy source than the control. In addition, modified TUJWT and DDT improved glucose tolerance without changing serum insulin levels during an oral glucose tolerance test. In conclusion, DDT have a better anti-obesity effect than TUJWT by decreasing energy intake in female rats with diet-induced obesity. It also improves glucose tolerance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.10
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• pp.3808-3819
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• 2010

This study was performed to determine the levels of quality of life(QOL) according to the grade of long-term care service in the elderly people who were judged from long-term care insurance, and to reveal its association with related variables. The interviews were made to 958 elderlies in urban and rural areas from March 1 to May 31, 2009. The mean scores of QOL among all subjects were $55.4{\pm}15.62$(Grade I; $49.7{\pm}14.17$, Grade II; $56.8{\pm}14.62$, Grade III; $59.4{\pm}16.36$), and they were lower according to the higher grade of long-term care insurance. The multiple regression analysis was used to reveal the explanatory powers of factors influencing on the level QOL. Such factors as educational level, monthly income, subjective health status, depression and MMSE-K were shown to affect their QOL in Grade I, Grade II, and Grade III with explanatory powers of 45~62%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.4
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• pp.581-587
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• 1996

This study was done to investigate the effects of Artemisia selengensis methanol extract on ethanol-induced hepatotoxicty in rat liver. Sprague-Dawley(SD) rats weighing about 150g were divided into the following 4 groups : control group(CON), Astemisia selengensis methanol extract administered group(ASE), ethanol adminstered group(ETH) and Artemisia selengenis methanol extract and ethanol administered group(ASA). Ethanol and Artemisia selengenis methanol extract were administered orally by 5m1/kg and 200mg/kg body weight per day for 6weeks, respectively. Body weight, daily food intake and percent liver weight per body weight were significantly changed by ethanol administration in comparison to control group. The activities of serum alanine aminotransferase(ALT), asparate aminotransferase(AST), and hepatic TBA-reactants increased by ethanol were decreased significantly by Artemisia selengensis methanol extract compared with ethanol group. It was also obseued that superoxide dismutase, catalase and glutathione peroxidase were not changed by Artemisia selengensis methanol extract, whereas hepatic xanthine oxidase activity was inhibitied by Artemisia selengensis methanol extract as compared to ethanol group. The glutathione contents in liver decreased by ethanol adminstration, but glutathione levels increased in ASA compared with ethanol group. These results suggest that Artemisia selengenis methanol extract have a possible protective effect on the ethanol-induced hepatotoxicity in rat liver.