• Molecules and Cells
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• v.43 no.5
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• pp.491-499
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• 2020

Hippo signaling acts as a tumor suppressor pathway by inhibiting the proliferation of adult stem cells and progenitor cells in various organs. Liver-specific deletion of Hippo pathway components in mice induces liver cancer development through activation of the transcriptional coactivators, YAP and TAZ, which exhibit nuclear enrichment and are activated in numerous types of cancer. The upstream-most regulators of Warts, the Drosophila ortholog of mammalian LATS1/2, are Kibra, Expanded, and Merlin. However, the roles of the corresponding mammalian orthologs, WWC1, FRMD6 and NF2, in the regulation of LATS1/2 activity and liver tumorigenesis in vivo are not fully understood. Here, we show that deletion of both Wwc1 and Nf2 in the liver accelerates intrahepatic cholangiocarcinoma (iCCA) development through activation of YAP/TAZ. Additionally, biliary epithelial cell-specific deletion of both Lats1 and Lats2 using a Sox9-Cre^ERT2 system resulted in iCCA development through hyperactivation of YAP/TAZ. These findings suggest that WWC1 and NF2 cooperate to promote suppression of cholangiocarcinoma development by inhibiting the oncogenic activity of YAP/TAZ via LATS1/2.

• Food Science of Animal Resources
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• v.41 no.4
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• pp.687-700
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• 2021

This study performed to evaluate the applicability of functional dairy food materials by comparing the calcium solubilization ability and anti-inflammatory effects of hydrolyzed casein protein. Commercial enzyme (Alcalase^®; Neutrase^®; Protamex^®; Flavourzyme^®) was added to the 10% casein solution to prepare the casein hydrolysates. Samples obtained every hour [1:200 (w/v)]. According to results of measuring the degree of hydrolysis (DH), all of four enzymatic hydrolysates increased rapidly from 30 to 40 min, and after 150 min, there were no change. Protamex^® and Neutrase^® had the highest DH compared to others enzymatic hydrolysates. After that, peptides obtained throughout a preparative liquid chromatography system. In the calcium solubility experiments, neutrase fraction (NF) 4 and NF7 showed similar activities with casein phosphopeptide (CPP). In vitro cell experiments showed that no cytotoxicity except for NF6. Also, the production of nitric oxide (NO) inhibited as the concentration of fraction samples increased. The cytokine (IL-1α, IL-6, and TNF-α) production was lower than lipopolysaccharide (+) group significantly. Therefore, the possibility of anti-inflammatory activity found in the hydrolyzed samples. According to the above experiments, NF3 and Protamex Fraction (PF) 3 selected. Amino acids selected throughout an AccQ-Tag system. As a result, 17 species of amino acids and several species of unknown amino acids identified. Both fractions had the highest content of phenylalanine. This study identified the potential of biologically active and functional peptides derived from casein that affect the food and dairy industry.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.43-49
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• 2001

We have cloned and characterized an immediate early-1 gene, iel, which is activated immediately upon entrance of the viral genome into the cell nucleus, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. This gene encodes a protein 584 amino acids with a predicted molecular weight of 67 kDa. The promoter and coding regions of BmNPV-K1 ie1 showed high homology with Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain. The BmNPV-K1 ie1 was different from amino acid sequence at 4 positions in BmNPV T3. The location of ie1 gene in the BmNPV-K1 genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were confirmed by Nerthern hybridization analysis.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.423-427
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• 2004

A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity with an overall yield of $80.1\%$ by ammonium sulfate precipitation and a single chromatographic step involving FPLCanion exchange chromatography. The purified hGM-CSF revealed at least five glycosylated forms ranging from $21.5{\~}29$ kDa, and its biological activity was independent of the glycosylation pattern. This is the first purification report of recombinant hGM-CSF to apparent homogeneity from rice cell suspension cultures.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.307-311
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• 2000

Abstract Extracellular chitinase was purified from the culture liquid of the marine bacterium, Bacillus sp. LJ-25 , and its enzymatic properties were examined. The purified chitinase exhibited a single band on SDS-PAGE and the molecular weight was estimated to be approximately 50 kDa. The optimum pH and temperature for the enzymatic activity were 7.0 and $35^{\circ}C$, respectively. The activity of the chitinase was strongly inhibited by $Zn^{2+}$ and slightly inhibited by $Ba^{2+},{\;}Co^{2+},{\;}Mn^{2+},{\;}and{\;}Cu^{2+}$. The purified chitinase did not hydrolyze $p-nitrophenolN-acetyl-{\bata}-D-glucosaminide{\;}(GlcNAc)_2$ and Micrococcus lysodeikticus cells, which are known to be the substrates for exo-type chitinase. Among the hydrolyzates of colloidal chitin, $(GlcNAc)_2$ was in the highest concentration with small amounts of GlcNAc and $(GlcNAc)_3$..

• Journal of the Korean Society for Precision Engineering
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• v.24 no.3 s.192
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• pp.84-90
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• 2007

As a result of vehicle maintenance of rocker arm shaft for 4-cylinder SOHC engine, failure stress analysis of rocker arm shaft is needed. Because more than 30% of vehicles investigated have been fractured. Failure stress analysis is classified into an naked eyes, microscope, striation and X-ray fractography etc. Failure stress analysis by using striation is already established technology as means for seeking cause of fracture. But, although it is well known that striation spacing corresponds to the crack growth rate da/dN, it is not possible to determine ${\sigma}_{max}\;and\;{\sigma}_{min}$ under service loading only from striation spacing. This is because the value of striation spacing is influenced not only by ${\Delta}K$ but also by the stress ratio R. In the present paper, we determine the stress ratio using orthogonal array and ANOVA, and propose a prediction method of failure stress which is combined with FEM and striation.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11a
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• pp.988-990
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• 2005

본 논문에서는 그리드 컴퓨팅 환경에서 사용자와 자원 제공자간의 자원거래 시 사용자의 자원 요구량을 예측하고, 합리적인 가격 결정 알고리즘을 이용하여 기존의 자원 거래 모델에 비해 빠른 응답 속도를 갖는 모델을 제안한다. 본 논문에서 제안하는 모델은 사용자의 자원 요구량를 예측을 위해 통계학의 예측 모델을 적용하였고, 그리드 자원의 거래 가격 결정을 위한 경제학의 이론을 도입하였다. 우리는 실험을 통해 기존의 모델들과 비교하여 그리드 자원 거래를 위한 응답시간을 비교 하였다. 우리는 실험을 통해 기존의 모델들과 비교하여 응답시간이 최소 $72.39\%$ 향상된 결과를 얻었고 우리가 제안한 모델이 기존 모델에 비해 우수하다는 것을 입증하였다.

• 한국HCI학회:학술대회논문집
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• 2006.02a
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• pp.1126-1129
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• 2006

최근 멀티플렉스 극장들이 많이 생기면서 영화 관객들이 한 장소에서 다양한 영화를 선택하여 감상할 수 있게 되었다. 그러나 극장에서 제공하는 영화정보는 비치된 팜플렛과 공중파 광고홍보가 유일하며 더 자세한 정보를 얻기 위해서는 인터넷이나 다른 매체를 통해 사전에 정보를 입수해야 하는 불편함이 존재하고 있다. 본 논문에서는 증강현실 기술을 이용하여 관객이 특정 마커가 부착된 영화팜플렛을 카메라에 비추면 마커에 해당되는 동영상을 찾아 재생하고 이때 스크린에 출력되는 동영상을 통해 영화의 예고편 및 등장인물에 대한 소개 등을 실시간으로 관객에게 제공하는 시스템을 구현한다. 본 시스템을 통해 관객에게 별도의 매체나 시간의 소비 없이 해당 극장에서 다양한 영화에 대한 정보를 쉽게 얻을 수 있고 이는 영화를 선택하는데 있어서 많은 편리함을 제공할 뿐만 아니라 영화를 선택하는 과정에 있어 시각적인 재미를 함께 제공한다.

• IMMUNE NETWORK
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• v.1 no.1
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• pp.14-25
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• 2001

Background: Bone marrow stromal cells (BMSCs) express many cell surface molecules, which regulate the proliferation and differentiation of immune cells within the bone marrow. Methods: To identify cell surface molecules, which can regulate cell proliferation through cell interaction, monoclonal antibodies (MoAbs) against BMSCs were produced. Among them, 1H8 MoAb, which recognized distinctly an 80 kDa protein, abolished myeloma cell proliferation that was induced by co-culturing with BMSCs. Results: IL-6 gene expression was increased when myeloma or stromal cells were treated with 1H8 MoAb. In addition, the expression of IL-6 receptor and CD40 was up-regulated by 1H8 treatment, suggesting that the molecule recognized by 1H8 MoAb is involved in cell proliferation by modulating the expression of cell growth-related genes. Myeloma cells contain high levels of reactive oxygen species (ROS), which are related to gene expression and tumorigenesis. Treatment with 1H8 decreased the intracellular ROS level and increased PAG antioxidant gene concomitantly. Finally, 1H8 induced the tyrosine phosphorylation of several proteins in U266. Conclusion: Taken together, 1H8 MoAb recognized the cell surface molecule and triggered the intracellular signals, which led to modulate gene expression and cell proliferation.

• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.2061-2065
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• 2009

Two simple algorithm modifications are made to the THRASH data retrieval program with the aim of improving analysis speed for complex Fourier transform ion cyclotron resonance (FTICR) mass spectra. Instead of calculating the least-squares fit for every charge state in the backup charge state determination algorithm, only some charge states are pre-selected based on the plausibility values obtained from the FT/Patterson analysis. Second, a modification is made to skip figure-of-merit (FOM) calculations in the central m/z region between two neighboring peaks in isotopic cluster distributions, in which signal intensities are negligible. These combined modifications result in a significant improvement in the analysis speed, which reduces analysis time as much as 50% for ubiquitin (8.6 kDa, 76 amino acids) FTICR MS and MS/MS spectra at the reliability (RL) value = 0.90 and five pre-selected charge states with minimal decreases in data analysis quality (Table 3).