• KSBB Journal
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• 제8권2호
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• pp.178-184
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• 1993

Extractive fermentations with the extract of Jerusalem artichoke in an aqueous-two-phase-system of polyethyleneglycol(PEG) and dextran(Dx) were investigated to obtain the effects of composition of PEG and Dx on both fermentation ,characteristics and partition ratio of alcohol. The specific growth rate of K. Fraglis CBS 1555 increased with a decrease of concentration of PEG and Dx. It augmented along with concentration of initial sugar up to 80g/l but decreased thereafter. The specific production rate of alcohol showed a rising tendency up to 100g/lof initial sugar, whereafter represented a decreasing trend. The partition ratio of alcohol between two phases augmented according to decrease of Dx comic. and increase of PEG cone. regardless of initial sugar concentrations. The ratio, however, decreased with Increment of initial sugar concentration at constant composition of PEG and Dx. The partition coefficient of alcohol had an ascending effect to the increase of PEG cone, but it had little effect on the changes of concentrations of Dx and initial sugar. The present study suggests that the optimum composition of PEG and Dx in the aqueous-two-phase-system by extractive fermentation were around 6.5%(w/v) of PEG and 3%(w/v) of Dx in considerations of emulsion state, sedimentation and separation of two phases, alcohol partition ratio, and specific growth rate.

• 한국벤처창업학회:학술대회논문집
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• 한국벤처창업학회 2022년도 춘계학술대회
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• pp.129-134
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• 2022

This research investigates the effect of SMEs' digital transformation (DX) efforts in O2O platforms on customer engagement. Among the three DX stages (i.e., digitization, digitalization, and DX), this study focuses on digitalization, a practically viable DX phase for SMEs using O2O platforms. This study categorizes the DX efforts of SMEs into three: information diversity, responsiveness to customers, and the degree of functional use. To analyze the impact of these efforts on customer engagement, we conduct the zero-inflated negative binomial regression using the dataset of Naver Smartplace, one of the representative O2O platforms in South Korea. The analysis result confirms that all three factors have positive impacts on customer engagement. Therefore, this study demonstrates that employing O2O platforms can be an effective strategy for SMEs lacking resources to achieve successful DX.

• 열처리공학회지
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• 제36권6호
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• pp.396-401
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• 2023

Aus-tempering heat treatment is suitable for thin and small-sized in precision parts. However, the heat treatment process relies on the experience and skill of the operator, making it challenging to produce precision parts due to the cold forging process. The aims of this study is to explore suitable machine learning models using data from the aus-tempering heat treatment process and analyze the factors that significantly impact the mechanic properties (e.g. hardness). As a result, the study analyzed, from a machine learning perspective, how hardness prediction varies based on the quenching temperature, carbon (C), and copper (Cu) contents.

• 산업융합연구
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• 제20권1호
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• pp.23-33
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• 2022

본 연구는 제조기업의 디지털 전환(DX)을 위한 실행모형을 개발하기 위해 진행하였다. 이를 위하여 경영혁신과 디지털전환 과정에 대한 선행연구를 검토하였다. 인터넷 비즈니스의 혁신과정에서 적용된 NEBIC이론과 혁신이론을 기반으로 DX과정모형을 도출하였다. 여기에 최고경영층(TMT)의 추진의지 요인을 포함한 연구모형을 구축하고 이를 검증하기 위해 험자료를 통해 확인하였다. 디지털 전환을 추진하고 있는 중견 제조기업의 구성원을 대상으로 수집된 자료를 기반으로 연구가설을 검증하였다. 회귀분석을 통해 연구모형(기술지식 → 기회인지 → 기대성과 → 실행의지)의 각 단계의 영향관계를 확인하였다. DX과정에 최고경영층의 DX추진의지에 대한 구성원의 인식의 매개효과를 파악하기 위해 위계적 회귀분석을 실시였다. Sobel test 확인결과, 각 단계별 관계에 경영층의 DX추진에 대한 인식이 부분적으로 매개하는 사실을 확인하였다. 본 연구는 중견 제조업의 디지털 전환에 적용 가능한 모형을 제시했다는 점에서 의미가 있다. 또한 혁신연구 및 NEBIC 확장에 대한 경험적 근거를 제공한 것에 가치를 지닌다. 동적특성을 지닌 과정모형에 대한 실증자료의 한계를 극복하기 위해서 종단연구가 필요하며 연구결과의 일반화를 위해 제조업 이외 다양한 분야에서 확장된 실증연구가 요구된다.

• Journal of Ginseng Research
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• 제21권3호
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• pp.160-168
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• 1997

The present study was carried out to investigate the effects of Panax ginseng saponin extracts on the dexamethasone-induced apoptosis of mouse thymus in vivo and mouse thymocytes in vitro. The saponin fractions of red ginseng (R-SAP) and white ginseng (Wl-SAP) were provided by the Korea Ginseng & Tobacco Research Institute, and the other saponin fraction of white ginseng (W2-SAP) was extracted in our laboratory. 1. The male ICR mice (3~4 wk old; weighing 15$\pm$2 g) were given by each saponin fraction of 5 mg/kg/ day for 4 days, and at one hour after the last treatment, they were injected by deuamethasone (5 mg/kg : DX). The mouse thymus was extracted at 6 hours after DX injection, and they were stained with hematoxylin-eosin reagents and an Apop-Tag kit, respectively, and the thymocytes prepared from it were labelled with anti-mouse FITC-anti-CD4 and anti-mouse PE-anti-CD8 and then analyzed by fluorescence activated cell sorter (FACS). DX-induced reduction of thymus weight was significantly attenuated by W2- SAP but was not affected by other saponin fractions. And DX-induced apoptotic death of thymocytes, appeared in the histologic findings of the thymus, was inhibited by the saponin fractions and the order of these inhibitory potencies was R-SAP》W2-SAP>Wl-SAP. However, in respect of T cell receptors, the differentiation of thymocytes seems not to be changed by treatments with DX or/and the saponin fractions. 2. In the primary thymocyte culture, the DX-induced reduction of thymocyte MTT values was rather greater in RPMI 1640 medium of IWc fetal bovine serum (FBS) or horse serum (HS). In addition, the DX-Induced MTT reduction was significantly inhibited by R-SAP or W2-SAP, in the culture using that medium of 5% FBS or HS. But these saponin fraction did not effected the DX-induced reduction of thymocyte MTT value in primary culture of 10% FBS or 10% HS. These results suggest that R-SAP and some W-SAP fractions may protect thymocyte from stress or glucocorticoisteroid-induced death of them.

• 정보보호학회논문지
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• 제2권1호
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• pp.45-48
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• 1992

제목에 주어진 타원 곡선들 $Y^{2}$=X$^{3}$+DX 은 허수 승법을 가진다. 이곡선을 소수 증명과 소인수 분해에 응용하는 방법을 이 논문에서 고찰해 본다.

• The Korean Journal of Physiology and Pharmacology
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• 제6권4호
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• pp.215-223
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• 2002

The present study was undertaken to investigate the effect of doxorubicin (DX) on secretion of catecholamines (CA) evoked by ACh, high $K^+,$ DMPP and McN-A-343 from the isolated perfused rat adrenal gland and to establish the mechanism of its action. DX $(10^{-7}{\sim}10^{-6}\;M)$ perfused into an adrenal vein for 60 min produced relatively dose- and time-dependent inhibition of CA secretory responses evoked by ACh $(5.32{\times}10^{-3}\;M),$ DMPP $(10^{-4}\;M)$ and McN-A-343 $(10^{-4}\;M).$ However, lower dose of DX did not affect CA secretion by high $K^+\;(5.6{\times}10^{-2}\;M),$ but its higher doses depressed time-dependently CA secretion evoked by high $K^+.$ DX itself did also fail to affect basal CA output. In adrenal glands loaded with DX $(3{\times}10^{-7}\;M),$ CA secretory responses evoked by Bay-K-8644, an activator of L-type $Ca^{2+}$ channels and cyclopiazonic acid, an inhibitor of cytoplasmic $Ca^{2+}-ATPase$ were time-dependently inhibited. Furthermore, daunorubicin $(3{\times}10^{-7}\;M),$ given into the adrenal gland for 60 min, attenuated CA secretory responses evoked by ACh, high $K^+,$ DMPP and McN-A-343. Taken together, these results suggest that DX causes relatively dose- and time-dependent inhibition of CA secretory responses evoked by stimulation of cholinergic (both nicotinic and muscarinic) receptors from the isolated perfused rat adrenal gland. However, lower dose of DX did not affect CA secretion by high $K^+,$ and higher doses of DX reduced time-dependently CA secretion of high $K^+.$ It is thought that these effects of DX may be mediated by inhibiting both influx of extracellular calcium into the rat adrenomedullary chromaffin cells and intracelluar calcium release from the cytoplasmic store. Also, there was no difference in the mode of action between DX and daunorubicin in rat adrenomedullary CA secretion.

• Journal of Microbiology and Biotechnology
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• 제18권9호
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• pp.1599-1605
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• 2008

Biodistribution and lymphoscintigraphy of cyclosporine A (CyA) and technetium-99m ($^{99m}Tc$) were studied using ${^99m}Tc$-labeled dextran acetate (DxA) including CyA. DxA particles were prepared from dextran with acetic anhydride, and CyA was loaded into them. Lymphatic delivery of ${^99m}Tc$-labeled DxA particles containing CyA was evaluated after subcutaneous injection into the foot pad of rats and compared with those of ${^99m}Tc$-labeled human serum albumin (HSA). The labeling efficiency of CyA-loaded ${^99m}Tc$-DxA particles was about 95% at 30 min. The labeling efficiency maintained stably above 80% for 12 h. The percent injected dose (%ID) of CyA-loaded ${^99m}Tc$-DxA was similar to that of ${^99m}Tc$-HSA at the inguinal lymph node after 40 min. The CyA-loaded ${^99m}Tc$-DxA could be as well distributed as ${^99m}Tc$-HSA through the lymph node. The DxA particles could steadily distribute the CyA as well as the ${^99m}Tc$ radiolabeling through the lymph node.

• 생명과학회지
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• 제8권4호
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• pp.400-409
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• 1998

Formation of adduct was studied in benzo(a)pyrene(BP)- and doxorubicin(Dx)-treated human NC-37 cells and isolated nuclei. Major adducts formed were determined by fluorescence absorption spectrophotometery and DNA-lin-ked protein assay. When isolated nuclei were exposed to carcinogens BP and DMBA, and anticancer drugs m-AMSA, ellipticine and Dx, varying degrees of adduct formation occured between DNA-protein complex and these drugs. When the mixture was centrifuged 1.7 M sucrose solution, binding BP and DMBA appeared to be similar between the sediment and the supernatant. When the sediment was centrifuged again with 0.35% polymin-P, the amount of BP bound was 2-fold greater in the protein(1077$\pm$55cpm) than in DNA fraction (470$\pm$20cpm), whereas that of DMBA was 1.6-fold greater in the DNA than in protein fraction. In the case of m-AMSA, ellipticine and Dx, the amount of binding was slightly greater in supernatant than in sediment in centrifugation with 1.7 M sucrose, and more than 3 times greater in the DNA- than in protein- fraction in centrifugation with 0.35% polymin P. DNA fractions which associated with a subset of nonhistone chromosomal protein were isolated from NC-37 cells exposed to $^{3}$H-BP and $^{14}$C-Dx. They were separated into two distince components DNA-S and DNA-P by centrifugation with 2M Nacl chromatin extraction. The results indicated that the amount of $^{3}$H-BP bound was 6.0-fold greater in DNA-P as compared with DNA-S, while that of $^{14}$C-Dx binding appreaed to be 6.2-fold greater in DNA-S than in DNA-P fraction. When $^{3}$H-BP binding wasdetermined in the presence of cold Dx, the amount of binding was reduced only in the DNA-P fraction, indicating that the interaction between DNA and protein is decreased. Gene expression by these drugs, BP treated cells were increased to compare with nomal cells but reduced by treatment with BP-Dx. These results suggest that the protein moiety which tightly bound to DNA-P fraction may play an important role in the regulation of gene expression.

• 한국식품과학회지
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• 제21권2호
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• pp.212-217
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• 1989

무우 및 고구마를 열풍건조하고 건조가 진행됨에 따라 표면적 변화를 측정하여 실험치와 잘 일치하는 건조수축식과 총괄적 건조 속도식을 구하였으며, 시료의 두께, 상대습도 및 풍속을 포함한 초기 건조속도의 함수관계 식을 구하였다. 건조속도 상수와 차수는 시료의 두께와 공기의 온도에 영향을 받았으며 일정한 건조 (d=4mm, $Ta=50^{\circ}C$, RH=10%, U=0.8m/s) 하에서 얻어진 총괄적 건조속도 식과 수축식은 무우는 $dx/dt=0.112{\times}10^{-2}{\cdot}A{\cdot}:(1-x)^{0.43}$, A=Ao(-0.480x+1)이었고, 고구마는 $dx/dt=0.115{\times}10^{-2}\;A(1-x)^{0.49}$, A=Ao(-0.368x+1)이었다. 또한 초기 건조속도의 변화는 시료의 두께, 상대습도, 및 풍속에 대해 무우는 $dx/dt=0.0648\;(RH)^{-0.31}\;(d)^{-0.75}\;(U)^{0.39}$이었고, 고구마는 $dx/dt=0.0547(RH)^{-0.28}\;(d)^{-0.63}\;(U)^{0.37}$이었다.