• BMB Reports
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• 제43권9호
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• pp.609-613
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• 2010

The Dvl and Axin proteins, which are involved in the Wnt signaling pathway, each contain a conserved DIX domain in their sequences. The DIX domain mediates interaction between Dvl and Axin, which together play an important role in signal transduction. However, the extremely low production of DIX domain fragments in E. coli has prevented more widespread functional and structural studies. In this study, we demonstrate that the DIX domains of Dvl and Axin are expressed noticeably in a multi-cistronic system but not in a mono-cistronic system. Formation of the $DIX_{Dvl1}-DIX_{Axin1}$ complex was investigated by affinity chromatography, SEC and crystallization studies. Unstable DIX domains were stabilized by complexing with counterpart DIX domains. The results of the preliminary crystallization and diffraction of the $DIX_{Dvl1}-DIX_{Axin1}$ complex may prove useful for further crystallographic studies.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2385-2390
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• 2015

Background: Invasive ductal (IDC) and lobular (ILC) carcinomas are the common histological types of breast carcinoma which are difficult to distinguish when poorly differentiated. Discoidin domain receptor (DDR1) and Drosophila dishevelled protein (DVL1) were recently suggested to differentiate IDC from ILC. Objectives: To assess the expression of DDR1 and DVL1 and their association with histological type, grading and hormonal status of IDC and ILC. Materials and Methods: This cross sectional study was conducted on IDC and ILC breast tumours. Tumours were immunohistochemically stained for (DDR1) and (DVL1) as well as estrogen receptor (ER), progesterone receptor (PR) and C-erbB2 receptor. Demographic data including age and ethnicity were obtained from patient records. Results: A total of 51 cases (30 IDCs and 21 ILCs) were assessed. DDR1 and DVL1 expression was not significantly associated with histological type (p=0.57 and p=0.66 respectively). There was no association between DDR1 and DVL1 expression and tumour grade (p=0.32 and p=1.00 respectively), ER (p=0.62 and 0.50 respectively), PR (p=0.38 and p=0.63 respectively) and C-erbB2 expression (p=0.19 and p=0.33 respectively) in IDC. There was no association between DDR1 and DVL1 expression and tumour grade (p=0.52 and p=0.33 respectively), ER (p=0.06 and p=0.76 respectively), PR (p=0.61 and p=0.43 respectively) and C-erbB2 expression (p=0.58 and p=0.76 respectively) in ILC. Conclusions: This study revealed that DDR1 and DVL1 are present in both IDC and ILC regardless of the tumour differentiation. More studies are needed to assess the potential of these two proteins in distinguishing IDC from ILC in breast tumours.

• 제어로봇시스템학회논문지
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• 제19권9호
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• pp.774-781
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• 2013

The purpose of this paper is to design a DVL-RPM based VKF (Velocity Kalman Filter) design for a performance improvement underwater integrated navigation system. The proposed approach relies on a VKF, augmented by a altitude from Echo-sounder based switching architecture to yield robust performance, even when DVL (Doppler Velocity Log) exceeds the measurement range and the measured value is unable to be valid. The proposed approach relies on two parts: 1) Indirect feedback navigation Kalman filter design, 2) VKF design. To evaluate proposed method, we compare the results of the VKF aided navigation system with simulation result from a PINS (Pure Inertial Navigation System) and conventional INS-DVL method. Simulations illustrate the effectiveness of the underwater navigation system assisted by the additional DVL-RPM based VKF in underwater environment.

• 한국해양공학회지
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• 제33권5호
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• pp.470-478
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• 2019

Because the global positioning system (GPS) is not available in underwater environments, an inertial navigation system (INS)/doppler velocity log (DVL) integrated navigation system is generally implemented. In general, an INS/DVL integrated system adopts a loosely coupled method. However, in this loosely coupled method, although the measurement equation for the filter design is simple, the velocity of the body frame cannot be accurately measured if even one of the DVL transducer signals is not received. In contrast, even if only one or two velocities are measured by the DVL transducers, the tightly coupled method can utilize them as measurements and suppress the error increase of the INS. In this paper, a filter was designed to regenerate the measurements of failed transducers by taking advantage of the tightly coupled method. The regenerated measurements were the normal DVL transducer measurements and the estimated velocity in RPM. In order to effectively estimate the velocity in RPM, a filter was designed considering the effects of the tide. The proposed filter does not switch all of the measurements to RPM if the DVL transducer fails, but only switches information from the failed transducer. In this case, the filter has the advantage of being able to be used as a measurement while continuously estimating the RPM error state. A Monte Carlo simulation was used to determine the performance of the proposed filters, and the scope of the analysis was shown by the standard deviation ($1{\sigma}$, 68%). Finally, the performance of the proposed filter was verified by comparison with the conventional tightly coupled method.

• 한국정보통신학회논문지
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• 제19권11호
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• pp.2751-2757
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• 2015

유도제어 시스템 체계 개발의 초기단계에는 운용 효과도 도출 및 요구사항 적합성 검토를 통한 체계 개략사양도출을 위해 효과도 분석을 수행한다. 본 논문에서는 M&S (Modeling & Simulation)를 활용하여 항법센서 성능과 환경영향(조류의 세기와 방향)에 따른 유도제어 시스템의 목표점 도달 정확도에 대한 운용 효과도 분석을 수행한다. 효과도 분석을 위해 6자유도 운동모델, 환경모델, 유도항법제어모델을 구성한다. 항법센서는 관성항법센서(Inertia Navigation Sensor, INS)와 도플러 속도센서(Doppler Velocity Log, DVL)로 구성하고, 환경변수는 조류(current)의 세기와 방향이다. 수치 시뮬레이션 결과는 CEP(Circular Error Probability)와 분산을 이용한 확률분석으로 분석한다. 효과도 분석 결과는 항법센서의 가격을 고려한 비용 대비 효율 분석에 활용하여 가격 대비 높은 성능의 센서 사양을 도출할 수 있다. 본 논문에서는 높은 수준의 INS와 낮은 수준의 DVL을 이용하면 가격 대비 성능이 높은 복합항법센서를 구성한다는 것을 보여준다.

• 한국정보통신학회논문지
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• 제10권11호
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• pp.1973-1977
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• 2006

본 논문에서는 IMU(Inertial Motion Unit), DVL(Doppler Velocity Log), USBL(Ultra Short Base Line) DGPS(Differential Global Positioning System) 등의 센서로부터 취득된 데이터를'융합하여 ROV(Remotely Operated Vehicle)와 AUV(Autonomous Underwater Vehicle)와 같은 수중체의 위치를 지구 전체영역에서 추정하기 위한 기본적인 알고리즘을 다루고 있다. 본 논문에 소개된 알고리즘은 6,000m급 과학 조사용 심해무인잠수정인 해미래[1]의 수중 위치추적에 사용될 예정이다.

• Molecules and Cells
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• 제40권6호
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• pp.401-409
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• 2017

The primary cilium is a non-motile microtubule-based organelle that protrudes from the surface of most human cells and works as a cellular antenna to accept extracellular signals. Primary cilia assemble from the basal body during the resting stage ($G_0$ phase) and simultaneously disassemble with cell cycle re-entry. Defective control of assembly or disassembly causes diverse human diseases including ciliopathy and cancer. To identify the effective compounds for studying primary cilium disassembly, we have screened 297 natural compounds and identified 18 and 17 primary cilium assembly and disassembly inhibitors, respectively. Among them, the application of KY-0120, identified as Brefeldin A, disturbed Dvl2-Plk1-mediated cilium disassembly via repression of the interaction of $CK1{\varepsilon}-Dvl2$ and the expression of Plk1 mRNA. Therefore, our study may suggest useful compounds for studying the cellular mechanism of primary cilium disassembly to prevent ciliopathy and cancer.

• BMB Reports
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• 제51권9호
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• pp.425-426
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• 2018

As highly conserved signaling cascades of multicellular organisms, Wnt and Hippo pathways control a wide range of cellular activities, including cell adhesion, fate determination, cell cycle, motility, polarity, and metabolism. Dysregulation of those pathways are implicated in many human diseases, including cancer. Similarly to ${\beta}-catenin$ in the Wnt pathway, the YAP transcription co-activator is a major player in Hippo. Although the intracellular dynamics of YAP are well-known to largely depend on phosphorylation by LATS and AMPK kinases, the molecular effector of YAP cytosolic translocation remains unidentified. Recently, we reported that the Dishevelled (DVL), a key scaffolding protein between canonical and non-canonical Wnt pathway, is responsible for nuclear export of phosphorylated YAP. The DVL is also required for YAP intracellular trafficking induced by E-cadherin, ${\alpha}-catenin$, or metabolic stress. Note that the p53/LATS2 and LKB1/AMPK tumor suppressor axes, commonly inactivated in human cancer, govern the reciprocal inhibition between DVL and YAP. Conversely, loss of the tumor suppressor allows co-activation of YAP and Wnt independent of epithelial polarity or contact inhibition in human cancer. These observations provide novel mechanistic insight into (1) a tight molecular connection merging the Wnt and Hippo pathways, and (2) the importance of tumor suppressor contexts with respect to controlled proliferation and epithelial polarity regulated by cell adhesion.

• 한국해양공학회지
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• 제27권2호
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• pp.93-99
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• 2013

This paper describes a robust algorithm for an integrated underwater navigation system based on VKF (velocity Kalman filter). The proposed approach relies on a VKF, augmented by the altitude from an echo-sounder-based switching architecture to yield robust performance, even when DVL (Doppler velocity log) exceeds the measurement range and the measured value cannot be valid. The proposed approach relies on three parts: 1) PINS (pure inertial navigation system), 2) VKF design, and 3) VKF-aided integrated navigation filter design. To evaluate the proposed method, we compare the results of the VKF-aided navigation system with the simulation result from a PINS and conventional INS-DVL method.

• BMB Reports
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• 제38권2호
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• pp.243-247
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• 2005

Dishevelled (Dvl) is a positive regulator of the canonical Wnt signaling pathway, which regulates the levels of $\beta$-catenin. The $\beta$-catenin oncoprotein depends upon the association of Dvl and Axin proteins through their DIX domains, and its accumulation directs the expression of specific developmental-related genes at the nucleus. Here, the $^1H$, $^{13}C$, and $^{15}N$ resonances of the human Dishevelled 2 DIX domain are assigned using heteronuclear nuclear magnetic resonance (NMR) spectroscopy. In addition, helical and extended elements are identified based on the NMR data. The results establish a structural context for characterizing the actin and phospholipid interactions and binding sites of this novel domain, and provide insights into its role in protein localization to stress fibers and cytoplasmic vesicles during Wnt signaling.