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A High Essential Amino Acid Properties Rice Cultivar 'Haiami' (필수아미노산 고함유 신품종 '하이아미')

  • Hong, Ha-Cheol;Kim, Yeon-Gyu;Yang, Chang-Ihn;Hwang, Hung-Goo;Lee, Jeom-Ho;Lee, Sang-Bok;Choi, Yong-Hwan;Kim, Hong-Yeol;Lee, Kyu-Seong;Yang, Sae-Jun;Kim, Myeong-Ki;Jeong, O-Young;Cho, Young-Chan;Jeon, Yong-Hee;Choi, Im-Soo;Jeong, Eung-Gi;Oh, Sea-Kwan;O, Myeong-Gyu;Yea, Jong-Du;Shin, Young-Seoup;Kim, Jeong-Ju
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.543-548
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    • 2011
  • Haiami is a new Japonica rice variety developed from a cross between 'Jinmibyeo' TR treated with ethyl methane sulfonate (EMS) EMS and 5-methytryptophan, and 'Gyehwabyeo' in order to develop a new premium quality rice variety by a rice breeding team of National Institute of Crop Science, Rural Development Administration in 2008. This variety has about 138 days of growth duration from transplanting to harvesting in central plain area of Korea. The heading date of this vareity was on $15^{th}$, August. The 'Haiami' has good semi-elect plant type and resistant to lodging with strong culm. The number of panicles/hill of 'Haiami' is more than that of 'Hwaseongbyeo'. This variety shows slow leaf senescence and considerable tolerance to viviparous germination. It is susceptible to leaf blast, bacterial blight, and insect pests, but resistance to rice stripe virus. The milled rice of this variety exhibited translucent, clear non-glutinous endosperm and short grain shape. The essential amino acid properties of 'Haiami' have more than 31% that of 'Hwaseongbyeo' in polished rice. This variety has premium palatability of cooked rice. The yield performance of this rice cultivar was about 5.38 MT/ha in milled rice in local adaptability test for three years from 2006 to 2008. 'Haiami' is adaptable to central and southern plain areas of Korea.

Assessment of an Optimum Biochar Application Rate for Tomato(Solanum lycopersicum L.) Cultivation (토마토 재배를 위한 바이오차 최적시용 비율 평가)

  • Park, Do-Gyun;Hong, Seung-Gil;Jang, Eunsuk;Shin, Joung-Du
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.1
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    • pp.39-48
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    • 2019
  • Objective of this study was to evaluate an optimum biochar application rate and estimate the carbon sequestration based on the soil chemical properties and growth responses for biochar application during tomatoes cultivation. The treatments consisted of control as recommended application rates of fertilizers, 0.01%, 0.03%, 0.05%, and 0.07% of biochar application(w/w, biochar:soil). For effects of soil chemical properties, the $NO_3-N$contents in the soil were peaked at 9 days after transplanting. But there was not significant difference(p>0.05) among the treatments during cultivation periods. However, $NH_4-N$ contents in the biochar treatment were lower than the control until 14 days of transplanting. $P_2O_5$ contents in the biochar treatments were lower than that of the control until 19 days after transplanting except 0.01% of biochar application plot. $K_2O$ contents in soils treated with 0.01% and 0.03% of biochar were higher until 6 days after transplanting than that in the control. For N use efficiency of biochar application, it was observed that the 0.05% biochar application plot was highest among the treatments. The highest carbon sequestration was estimated at $2.83mg\;kg^{-1}$ for 0.03% of biochar application. However, it is considered that the optimum biochar application rate was 0.05% for tomato cultivation, considering the growth characteristics and yield components.

Investigation of an Optimum Application Rate of Blended Biochar Pellet as Slow Release Fertilizer during Cabbage Cultivation (배추재배 시 바이오차 펠렛 완효성 비료의 적정 시용량 구명)

  • Kim, HuiSeon;Yun, SeokIn;Jang, Eunsuk;Shin, JoungDu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.1
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    • pp.49-56
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    • 2019
  • This experiment was conducted to select an optimum application rate of blended biochar pellet as slow release fertilizer during cabbage cultivation. The blended biochar pellet made with a combination(4:6) of biochar and pig manure compost with unloading N, P, K solutions for adjusting about 9% of total nitrogen(TN). The treatments were consisted of the control as recommended application rates for cabbage cultivation in National Institute of Agricultural Sciences, N 40%, N 40% and 0.07M MgO and N 60 % of the blended biochar pellet, respectively, based on nitrogen application of recommended rates to cabbage cultivation. Changes of $NH_4-N$, $NO_3-N$, $P_2O_5$ and $K_2O$ concentrations in the soil and growth characteristic and yield components were investigated and observed during the cabbage cultivation. The experimental result shown that contents of $NH_4-N$, $NO_3-N$ and $K_2O$ of soil in the N 40% were significantly difference(p<0.01) with the control. $P_2O_5$ concentrations of soil in the N 40% were highest among the treatments. The fresh weight per cabbage in the N 40% was not significantly different(p>0.05) from the control, but in the N 40% and 0.07M MgO and N 60% was lower than that of the control. It was considered that an optimum blended biochar application rate for cabbage cultivation was 40% of recommended nitrogen application.

Anti-aging and Anti-inflammatory Activities of the Extracts of Calamagrostis arundinacea (Calamagrostis arundinacea (실새풀) 추출물의 항노화 및 항염증 활성)

  • Jeong, Hea Seok;Lee, Dong Ho;Lee, Min-Sung;Heo, Tae Im;Kim, Dong Kap;Oh, Seung Hwan;Kim, Du Hyeon;Kim, Yeong-Su;Kim, Dae Wook
    • Journal of Life Science
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    • v.31 no.3
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    • pp.298-304
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    • 2021
  • The anti-aging and anti-inflammatory activities of hot-water (Ca-HW) and 70% ethanol (Ca-E70) whole-plant Calamagrostis arundinacea extracts, as well as their bioactive potentials, were investigated using cell-free and cell-mediated experimental systems. Use of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical decolorization assay to evaluate the antioxidant activity of the Ca-HW and Ca-E70 extracts revealed DPPH radical scavenging activities of 27% and 48%, respectively. Neither extract caused significant cytotoxicity, and both showed cell proliferation and promotion effects using RAW 264.7, B16F10, and CCD986sk cells. B16F10 melanoma cells showed higher melanin synthesis when treated with 100 mg/ml Ca-HW or Ca-E70 than with arbutin, indicating a stronger inhibitory effect of arbutin on melanin synthesis. Ca-HW and Ca-E70 increased pro-collagen biosynthesis in the human fibroblast CCD986-SK cell line by 24.69% and 12.55%, respectively. Analysis of the anti-inflammatory effects of different concentrations of Ca-HW and Ca-E70 in RAW264.7 cells revealed that Ca-E70 appeared to inhibit the lipopolysaccharide-induced production of nitric oxide and IL-6, a proinflammatory cytokine; therefore, Ca-E70 showed an anti-inflammatory effect. These results suggested that C. arundinacea extracts could have skin anti-aging and anti-inflammatory properties.

A Study on the Painting's Aesthetic of Gongjae Yoon Duseo (공재(恭齋) 윤두서(尹斗緖)의 회화심미(繪畵審美) 고찰)

  • Kim, Doyoung
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.1
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    • pp.175-183
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    • 2021
  • Gongjae Yoon DuSeo(1668~1715), from Haenam in the late Joseon Dynasty, is a scholar-born painter who was active during King Sukjong. He is the person who created the foundation as a pioneer of realist paintings in the late Joseon period during the transition from the middle to the latter period. He was born in Namin's prestigious family, but he ended his career as part of a partisan fight and immersed himself in painting and learning. 18C, the beginning of the late Joseon Dynasty, was a period when Silhak emerged and the Jinkyung era opened with awareness of nationalism. At this time, by incorporating the Silhak thought into the art world, the real reformed aesthetic consciousness was demonstrated to pioneer common people's customs, the application of Western painting methods, the pursuit of realist techniques, and the introduction of Namjongmuninhwa. His view of painting, who thoroughly learned the old things and pursued change, must have both the form and spirit that he can achieve 'HwaDo' only when it has the science of 'learning and knowledge' and the technical elements of 'practice and quality' emphasized. He has worked in a variety of reconciliations. In particular, portrait paintings are characterized by ihyeongsasin's realistic expressions of aesthetics. His masterpiece, 「Self-portrait」, excels in extreme-realistic depiction and innovation in composition, and stands out with an unconventional experimentation spirit that expresses his mind and thoughts in a painting with a sense of resentment. His landscape paintings combine to express the form as it is and mental notions, and beautifully embodied Do as a form, thus achieving ihyeongmido, which reached the level of'joyfulness forgotten even the heart of joy'. On the other hand, the generalization of the common people using various common people's lives as the subject of an open-mindedness aimed at gaining the facts of ihyeongsajin, a passive protest against corrupt power and an expression of a spirit of love. Since then, his painting style has been passed down from generation to generation to his eldest son Yoon Deok-hee and his grandson Yoon Yong, leading the change and revival of calligraphy art in the late Joseon Dynasty.

Changes of Leaf Characteristics, Pigment Content and Photosynthesis of Forsythia saxatilis under Two Different Light Intensities (광량 차이에 의한 산개나리의 엽 특성과 광색소 함량 및 광합성 변화)

  • Han, Sim-Hee;Kim, Du-Hyun;Kim, Gil Nam;Byun, Jae-Kyung
    • Journal of Korean Society of Forest Science
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    • v.100 no.4
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    • pp.609-615
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    • 2011
  • Forsythia saxatilis is a Korean endemic plant designated as rare and endangered by the Korea Forest Service (KFS). Growth and physiological characteristics of F. saxatilis were investigated under two different light intensities in order to figure out an appropriate growth environment for conservation and restoration of the species in its natural habitat. Shoot length, leaf size and weight, photosynthetic pigment content and photosynthetic parameters were measured for F. saxatilis grown at two experimental plots under relative light intensities (RLI) of 20% and 60% of the full sun, respectively. Fresh leaf weight of plants grown under high relative light intensities (RLI-60) exceeded that of plants grown at 20% RLI. The ratio of fresh leaf weight to leaf size at RLI-60 was 1.47 times superior comparing to that recorded at RLI-20. The content of photosynthetic pigments such as chlorophyll a, b and carotenoid were higher in plants grown at RLI-60, whereas the ratio of total chlorophyll to carotenoid content was higher in the leaves at RLI-20. Photosynthetic rate, stomatal conductance and transpiration rate at RLI-60 were, respectively, 2.5, 2.65 and 1.79 times higher comparing to those recorded at RLI-20. Water use efficiency, however, was higher at RLI-20. The chlorophyll/nitrogen ratio was 1.83 times higher at RLI-20 than at RLI-60. In contrast, the ratio of net photosynthesis to chlorophyll content at RLI-60 was 2.58 times higher than that of RLI-20. In conclusion, light intensity might be the major factor affecting growth and physiological characteristics of F. saxatilis grown under canopy of tall tree species.

Trends in QA/QC of Phytoplankton Data for Marine Ecosystem Monitoring (해양생태계 모니터링을 위한 식물플랑크톤 자료의 정도 관리 동향)

  • YIH, WONHO;PARK, JONG WOO;SEONG, KYEONG AH;PARK, JONG-GYU;YOO, YEONG DU;KIM, HYUNG SEOP
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.26 no.3
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    • pp.220-237
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    • 2021
  • Since the functional importance of marine phytoplankton was firstly advocated from early 1880s massive data on the species composition and abundance were produced by classical microscopic observation and the advanced auto-imaging technologies. Recently, pigment composition resulted from direct chemical analysis of phytoplankton samples or indirect remote sensing could be used for the group-specific quantification, which leads us to more diversified data production methods and for more improved spatiotemporal accessibilities to the target data-gathering points. In quite a few cases of many long-term marine ecosystem monitoring programs the phytoplankton species composition and abundance was included as a basic monitoring item. The phytoplankton data could be utilized as a crucial evidence for the long-term change in phytoplankton community structure and ecological functioning at the monitoring stations. Usability of the phytoplankton data sometimes is restricted by the differences in data producers throughout the whole monitoring period. Methods for sample treatments, analyses, and species identification of the phytoplankton species could be inconsistent among the different data producers and the monitoring years. In-depth study to determine the precise quantitative values of the phytoplankton species composition and abundance might be begun by Victor Hensen in late 1880s. International discussion on the quality assurance of the marine phytoplankton data began in 1969 by the SCOR Working Group 33 of ICSU. Final report of the Working group in 1974 (UNESCO Technical Papers in Marine Science 18) was later revised and published as the UNESCO Monographs on oceanographic methodology 6. The BEQUALM project, the former body of IPI (International Phytoplankton Intercomparison) for marine phytoplankton data QA/QC under ISO standard, was initiated in late 1990. The IPI is promoting international collaboration for all the participating countries to apply the QA/QC standard established from the 20 years long experience and practices. In Korea, however, such a QA/QC standard for marine phytoplankton species composition and abundance data is not well established by law, whereas that for marine chemical data from measurements and analysis has been already set up and managed. The first priority might be to establish a QA/QC standard system for species composition and abundance data of marine phytoplankton, then to be extended to other functional groups at the higher consumer level of marine food webs.

Anti-Hemolytic and Antimicrobial Effects against Multidrug-Resistant Bacteria of Enterococcus faecalis Isolated from Human Breast Milk (모유에서 분리한 Enterococcus faecalis의 다제내성 균에 대한 항용혈 및 항균 효과)

  • Yi, Eun-Ji;Lee, Jeong-eun;Jo, So-Yeon;Kim, Soo-bin;Yu, Du-na;Kook, Moochang;Kim, Ae Jung
    • Microbiology and Biotechnology Letters
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    • v.49 no.4
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    • pp.519-527
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    • 2021
  • In this study, the hemolysis of Enterococcus faecalis BMSE-HMP strains, isolated from human breast milk, was investigated, and the anti-hemolytic and antimicrobial effects on multidrug-resistant (MDR) bacteria were investigated. The enzyme activity of E. faecalis BMSE-HMP 4 strains was measured, and it was found that the activities of esterase and esterase lipase were the highest. In addition, no hemolytic reaction was observed in any of the isolates. Subsequently, the anti-hemolytic activity against MDR strains causing hemolysis was evaluated. E. faecalis BMSE-HMP002 had the highest anti-hemolytic activity against Staphylococcus aureus CCARM 3855 at 75.71 ± 10.00%. The anti-hemolytic activity against Escherichia coli DC 2 CCARM 0238 and Pseudomonas aeruginosa CCARM 0223 showed that the activity of BMSE-HMP001 was highest at 76.92 ± 2.99% and 87.93 ± 1.93%, respectively. Examination of the antimicrobial effects against the MDR bacteria Staphylococcus spp., Escherichia spp., Pseudomonas spp., Salmonella spp., Klebsiella spp., Enterobacter spp., and E. faecalis BMSE-HMP strains showed antimicrobial effects against both gram-positive and gram-negative strains. Breastfeeding delivers enterococci into the intestinal tract of newborns by lactation, and its usefulness is attracting attention as it has been reported that enterococci have a potential effect on neonatal immune development. In this study, the hemolytic and antimicrobial effects of E. faecalis BMSE-HMP strains on MDR bacteria were investigated, to confirm their potential as useful lactic acid bacteria. Additional studies on the antibiotic resistance and toxicity of the E. faecalis BMSE-HMP strains, isolated in this study, are necessary to prove it safe for use.

Effect of Pre-harvest Irradiation of UV-A and UV-B LED in Ginsenosides Content of Ginseng Sprouts (새싹 인삼의 수확 전 UV-A 및 -B LED의 조사에 의한 진세노사이드의 영향)

  • Jang, Seong-Nam;Lee, Ga-Oun;Sim, Han-Sol;Bae, Jin-Su;Lee, Ae-Ryeon;Cho, Du-Yong;Cho, Kye-Man;Son, Ki-Ho
    • Journal of Bio-Environment Control
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    • v.31 no.1
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    • pp.28-34
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    • 2022
  • This study was conducted to determine the changes in ginsenosides content according to additional UV-A, and UV-B LED irradiation before harvesting the ginseng sprouts. One-year-old ginseng seedlings (n=100) were transplanted in a tray containing a ginseng medium. The ginseng sprouts were grown for 37 days at a temperature of 20℃ (24h), a humidity of 70%, and an average light intensity of 80 µmol·m-2·s-1 (photoperiod; 24h) in a container-type plant factory. Ginseng sprouts were then transferred to a custom chamber equipped with UV-A (370 nm; 12.90 W·m-2) and UV-B (300 nm; 0.31 W·m-2) LEDs and treated for 3 days. Growth parameters and ginsenoside contents in shoot and root were conducted by harvesting on days 0 (control), 1, 2, and 3 of UV treatments, respectively. The growth parameters showed non-significant differences between the control and the UV treatments (wavelengths or the number of days). Ginsenoside contents of the shoot was highly improved by 186% in UV-A treatment compared to the control in 3 days of the treatment time. The ginsenoside contents of the roots was more improved in UV-A 1-day treatment and UV-B 3-day treatment, compared to the control by 171% and 160%, respectively. As a result of this experiment, it is thought that UV LED irradiation before harvesting can produce sprout ginseng with high ginsenoside contents in a plant factory.

Comprehensive comparison of nutritional constituents and antioxidant activity of cultivated ginseng, mountain-cultivated ginseng, and whole plant parts of mountain-cultivated ginseng (인삼, 산양삼 및 산양삼 전초의 영양성분 및 항산화 활성의 종합적인 비교)

  • Lee, Hee Yul;Jung, Jea Gack;Kim, Su Cheol;Cho, Du Yong;Kim, Min Ju;Lee, Ae Ryeon;Son, Ki-Ho;Lee, Jin Hwan;Lee, Dong-Hee;Cho, Kye Man
    • Journal of Applied Biological Chemistry
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    • v.64 no.4
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    • pp.453-463
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    • 2021
  • This study investigated that comparison of the nutrients (including fatty acids, amino acids, and minerals) and ginsenoside, total phenolic (TP) and total flavonoid (TF) contents and antioxidant activities in 5-year-old cultivated ginseng (CG), mountain-cultivated ginseng (MCG), and whole plant parts of MCG (WPMCG). The contents of total fatty acids and unsaturated fatty acids were determined highest in MCG (1215.9 and 751.2 mg/100 g), and saturated fatty acid was determined highest in WPMCG (486.4 mg/100 g) respectively. Characteristically, the α-linolenic acid content was detected up to 10 times higher in MCG (139.3 mg/100 g) and WPMCG (194.4 mg/100 g) compared to CG (18.1 mg/100 g). Total free and essential amino acid contents were high with CG < MCG < WPMCG (1006.35 mg/100 g and 839.46 mg/100 g). Notably, γ-aminobutyric acid and arginine were detected as the main non-essential amino acids with highest levels detected in WPMCG (163.10 mg/100 g) and MCG (305.23 mg/100 g), respectively. Total mineral content was high in CG (30.36 mg/100 g) and WPMCG (29.82 mg/100 g). Particularly, Calcium (Ca) was detected more than twice as high in WPMCG (6.68 mg/100 g) as compared to CG and MCG. TP and TF contents were 5.12 gallic acid mg/g and 3.04 RE mg/g respectively. Ginsenoside content was the highest in WPMCG (42.44 mg/g) in general and the highest antioxidant activity was also observed in WPMCG.