• 펄프종이기술
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• 제30권4호
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• pp.1-11
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• 1998

• Journal of Acupuncture Research
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• 제28권3호
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• pp.101-110
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• 2011

목적 : 이 연구는 봉독이 NF-${\kappa}$B의 활성억제를 통하여 전립선 암세포주인 DU-145 세포의 성장을 억제하 는지를 확인하고 그 기전을 살펴보고자 하였다. 방법 : 봉독을 처리한 후 DU-145의 성장억제를 관찰하기 위해 WST-1 assay를 시행하였고, 세포자멸사의 관찰에는 DAPI staining assay를 통한 세포형태관찰을 시행하였으며, 염증관련유전자 발현 관찰에는 western blot analysis를 시행하였고, 세포자멸사와 연관된 NF-${\kappa}$B의 활성 변화를 관찰하기 위해 EMSA와 luciferase assay를 시행하였으며, DU-145에서 봉독과 NF-${\kappa}$B의 상호작용을 관찰하기 위해 transient transfection assay를 시행하여 세포생존율과 NF-${\kappa}$B의 활성 변동을 측정하였다. 결과 : DU-145 세포에서 봉독을 처리한 후 세포성장이 억제되었으며, 염증관련유전자 발현 및 NF-${\kappa}$B의 활성의 유의한 감소를 나타내었다. DU-145 세포에서 NF-${\kappa}$B의 p50와 IKK들을 치환하여 작용기를 없애고 봉독을 처리하였을 경우에도 세포활성 및 NF-${\kappa}$B의 활성의 유의한 감소를 나타내었다. 결론 : 이상의 결과는 봉독이 NF-${\kappa}$B의 활성 억제를 통하여 인간 전립선암세포주인 DU-145의 세포자멸사를 유발함으로써 증식억제 효과가 있음을 입증한 것으로 전립선암의 예방과 치료에 대한 효과적인 치료제 개발에 도움이 될 것으로 기대된다. 다만 그 기전에서 봉독은 기존연구와 같은 NF-${\kappa}$B p50 및 IKK들의 작용기와 상호작용 이외에 다른 기전이 관여되는 것으로 심화 연구를 요한다.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1281-1290
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• 2015

Thermolysin and its homologs are a group of metalloproteases that have been widely used in both therapeutic and biotechnological applications. We here report the identification and characterization of a novel thermolysin-like protease, BtsTLP1, from insect pathogen Bacillus thuringiensis serovar Sichuansis strain MC28. BtsTLP1 is extracellularly produced in Bacillus subtilis, and the active protein was purified via successive chromatographic steps. The mature form of BtsTLP1 has a molecule mass of 35.6 kDa as determined by mass spectrometry analyses. The biochemical characterization indicates that BtsTLP1 has an apparent K_m value of 1.57 mg/ml for azocasein and is active between 20℃ and 80℃. Unlike other reported neutral gram-positive thermolysin homologs with optimal pH around 7, BtsTLP1 exhibits an alkaline pH optimum around 10. The activity of BtsTLP1 is strongly inhibited by EDTA and a group of specific divalent ions, with Zn²⁺ and Cu²⁺ showing particular effects in promoting the enzyme autolysis. Furthermore, our data also indicate that BtsTLP1 has potential in cleaning applications.

• Journal of Acupuncture Research
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• 제26권3호
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• pp.49-58
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• 2009

목적 : 본 연구는 봉약침의 주요성분인 멜리틴이 전립선 암세포주인 DU-145 세포성장에 어떤 영향을 미치는지를 알아보기 위하여 시행하였다. 방법 : 멜리틴이 DU-145의 성장에 미치는 영향을 알아보기 위한 cell viability 측정으로는 WST-1 assay를, 세포자멸사의 관찰에는 DAPI(4,6-diamidino-2-phenylindole)와 TUNEL staining assay를 시행하였으며, 세포자멸사 조절단백질(calpain, Bax, caspase-3, -9, cleaved caspase-3, cleavaged PARP, cleaved caspase-9, Bcl-2, XIAP, cIAP2, Akt, p-Akt, MMP-2, MMP-13)의 관찰을 위하여 western blot analysis를 시행하였다. 결과 : 1. DU-145 세포에서 멜리틴을 처리한 후 세포자멸사가 유도되어 세포성장이 억제되었다. 2. 세포자멸사 관련 단백질 중 분리된 caspase-3, caspase-9은 유의한 증가를, Bcl-2, p-Akt, XIAP, cXIAP는 유의한 감소를 나타내었다. 결론 : 이상의 결과는 멜리틴이 인간 전립선암세포주인 DU-145의 세포자멸사를 유발함으로써 증식억제 효과가 있음을 나타낸 것으로, 전립선암의 예방과 치료에 대한 효과적인 치료제 개발에 도움이 될 것으로 기대된다.

• 미술이론과 현장
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• 제6호
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• pp.51-76
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• 2008

Nous obserbons dans le champs de l'art aujourd' hui, le changement radical provoquant $\'{e}$tant donn$\'{e}$ que le pouvoir du march$\'{e}$ de l'art s'augmente de plus en plus. C'est pour cela que le champ du discours de l'art n'est actuellement plus form$\'{e}$ par des notions esth$\`{e}$tiques artistiques, mais par celles qui sont r$\'{e}$serv$\'{e}$es au domaine de l'$\'{e}$conomie et du march$\'{e}$. Il n'est plus significatif de croire la valeur esth$\`{e}$tique de l'oeuvre d'art, puisque l'esth$\`{e}$tique elle-m$\^{e}$me ne fonctionne plus. Par contre, le syst$\`{e}$me de donner le prix $\`{a}$ l'oeuvre d'art fonctionne rigoureusement. En plus, cette situation s'aggrave $\'{e}$tant donn$\'{e}$ que la mondialisation du march$\'{e}$ de l'art s'installe avec rapidit$\'{e}$. Malgr$\'{e}$ la mondialisation de l'art, des gens qui habitent par exemple $\`{a}$ S$\'{e}$oul ne peuvent pas s' approcher facilement de ce qui se passe $\`{a}$ New York, le vrai centre de l'art contemporain. Dans cette situation, le champ de discours de l'art n'a pas d'autre solution de se r$\'{e}$tr$\'{e}$cir remaquablement et le sujet de discussion sur le grand art est remplac$\'{e}$ par celui du success de l'art. Cette recherche est arriv$\'{e}$e $\`{a}$ remarquer que le syst$\`{e}$me du foire internationale de l'art fonctionne comme une locomotive dans le march$\'{e}$ de l'art qui est le vrai dominateur de l'art contemporain. Aujourd'hui les grands clients du monde de l'art et les jeunes sp$\'{e}$ctateurs, les clients de l'avenir s'assemblent autour de quelques foires internationales r$\'{e}$put$\'{e}$s qui ont de l'autorit$\'{e}$. C'est ainsi que l'achat et la vante de l'oeuvres d'art est suffisamment mondialis$\'{e}$ et que le syst$\`{e}$me local du march$\'{e}$ est d$\'{e}$rang$\'{e}$ et s'$\'{e}$ffondr$\'{e}$gravement. Malgr$\'{e}$ la diff$\'{e}$rence significative, la majorit$\'{e}$ des galeries d'art lacaux s'oblige de suivre $\`{a}$ la suite du style du foire internationale de l'art. C'est la raison pour laquelle que cette recherche se fait une id$\'{e}$e nette que la prosp$\'{e}$rit$\'{e}$ actuelle du march$\'{e}$ de l'art n'est que la situation $\'{e}$conomique ou financi$\`{e}$re. A ce stade de la r$\'{e}$flexion, cette recherche arrivera $\`{a}$ poser une question primordiale ci-dessous: "Si l'art qu'on $\`{a}$ l'intention de proclamer ne serai plus possible de faire r$\'{e}$fl$\'{e}$chir sur la libert$\'{e}$ et la solidarit$\'{e}$ humain, la dignit$\'{e}$ de l'homme, cet art n'est plus autre chose que l'insignifiance. Au lieu de parcourir divers ph$\'{e}$nom$\`{e}$ne du march$\'{e}$ de l'art aujourd'hui, cette recherche fixera son attention sur la r$\'{e}$alit$\'{e}$ du foire internationales de l'art. Dans ce context, cette recherche sera compos$\'{e}$e par trois chapitres donc le premi$\`{e}$re essayera de r$\'{e}$pondre $\'{a}$ la question de son influence extraodinairement puissante. Le deuxi$\`{e}$me chapitre essayera d'analiser la r$\'{e}$alit$\'{e}$ et la source du pouvoir du foire internationale de l'art. Nous essayeront au troisi$\`{e}$me chapitre de configurer le point de vue critique qui permettra de bien comprendre notre sujet.

• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.3113-3121
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• 2014

Colorectal cancer remains one of the most common types of cancer and a leading cause of cancer death worldwide. In this study, we aimed to investigate effects of DuP-697, an irreversible selective inhibitor of COX-2 on colorectal cancer cells alone and in combination with a promising new multi-targeted kinase inhibitor E7080. The HT29 colorectal cancer cell line was used. Real time cell analysis (xCELLigence system) was conducted to determine effects on colorectal cell proliferation, angiogenesis was assessed with a chorioallantoic membrane model and apoptosis was determined with annexin V staining. We found that DuP-697 alone exerted antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. For the antiproliferative effect the half maximum inhibition concentration ($IC_{50}$) was $4.28{\times}10^{-8}mol/L$. Antiangiogenic scores were 1.2, 0.8 and 0.5 for 100, 10 and 1 nmol/L DuP-697 concentrations, respectively. We detected apoptosis in 52% of HT29 colorectal cancer cells after administration of 100 nmol/L DuP-697. Also in combination with the thyrosine kinase inhibitor E7080 strong antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells were observed. This study indicates that DuP-697 may be a promising agent in the treatment of colorectal cancer. Additionally the increased effects observed in the combination with thyrosine kinase inhibitor give the possibility to use lower doses of DuP-697 and E7080 which can avoid and/or minimize side effects.

• 영어영문학
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• 제55권5호
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• pp.907-936
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• 2009

Quite arguably, W. E. B. Du Bois is the first figure in the history of black nationalism who engaged most persistently and systematically with the dominant ideology of racism and white supremacy. It is not too much to say that, by contending with the Eurocentric but taken-for-granted concept of the 'Negro' in the turn of the century, Du bois has laid the theoretical and ideological cornerstone of postcolonialism today. But his concept of race varied over time and was even contradictory in the same writings. The early Du Bois defined race as something historically made rather than biologically given and determined. Yet he didn't utterly deny the significance of physical traits and skin color in constructing racial identity. His notion of the 'Negro' was not unambiguous, either. While drawing on the 'soul' of 'black folk' to undermine the Eurocentric dichotomy of white/mind and black/body, Du Bois argued that there is some kind of 'spiritual' differences between whites and blacks, differences that are essentially inherent and hereditary in the 'Negro.' Such essentialist notion of race and the 'Negro' was on the wane in the later Du Bois, especially after his encounter with Marxism. He came to think of race merely as a discourse of racism that can be subverted and even appropriated for anti-racist practices. Following the Marxist assumption that 'the color line' is a class conflict on the international level, Du Bois contended that the 'Negro' is an outcome of slavery which is in turn a subsystem of Western capitalism. He also argued that, since the 'Negro' is not a biological essence but a sociocultural formation, the identity of the 'Negro' can and must be reconstructed according to historical change. For Du Bois, therefore, the resistance against colonialism and capitalism became a resistance against racism. This is why his Pan-African movement shifted its gear from the American program in the initial phase to a truly 'Afrocentric' and socialist one.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.191-192
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• 1989

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권1호
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• pp.340-343
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• 2007

We describe the DuPont Displays full color OLED printing and materials technologies. The process is more cost-effective and scalable than thermal evaporation through shadow masks. The materials lifetime is sufficient for most portable applications and is nearing that required for stationary displays. Recently, 4.3" WQVGA displays were demonstrated.

• 생약학회지
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• 제41권4호
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• pp.233-237
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• 2010

The crude drug 'CheonKun' is derived from the underground portions of Rubia species. However, the aerial parts of the plants are also sold under the 'GgogDuSeoNiIp' in Korea. To clarify the botanical origin of 'GgogDuSeoNiIp' the morphological and anatomical characteristics of the leaves of Rubia species growing in Korea, i.e. Rubia akane, R. chinensis var. glabrescens, R. cordifolia var. pratensis were compared. As a result, it was determined that 'GgogDuSeoNiIp' was the leaf of Rubia akane and R. cordifolia var. pratensis.