• International Journal of Oral Biology
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• 제43권3호
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• pp.133-140
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• 2018

Resveratrol (3,4',5,-trihydroxystilbene), a phytoalexin present in grapes, exerts a variety of actions to reduce superoxides, prevents diabetes mellitus, and inhibits inflammation. Resveratrol acts as a chemo-preventive agent and induces apoptotic cell death in various cancer cells. However, the role of resveratrol in odontoblastic cell differentiation is unclear. In this study, the effect of resveratrol on regulating odontoblast differentiation was examined in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. Resveratrol significantly accelerated mineralization as compared with the control culture in differentiation of MDPC-23 cells. Resveratrol significantly increased expression of ALP mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly accelerated expression of Col I mRNA as compared with the control in differentiation of MDPC-23 cells. Resveratrol significantly increased expressions of DSPP and DMP-1 mRNAs as compared with the control in differentiation of MDPC-23 cells. Treatment of resveratrol did not significantly affect cell proliferation in MDPC-23 cells. Results suggest resveratrol facilitates odontoblast differentiation and mineralization in differentiation of MDPC-23 cells, and may have potential properties for development and clinical application of dentin regeneration materials.

• International Journal of Oral Biology
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• 제32권1호
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• pp.13-22
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• 2007

The stem cell research is emerging as a cutting edge topic for a new treatment for many chronic diseases. Recently, dental stem cell would be possible for regeneration of tooth itself as well as periodontal tissue. However, the study of the cell characterization is scarce. Therefore, we performed the genetic profiling and the characterization of mouse fetus/neonate derived dental tissue and cell to find the identification during dental development. We separated dental arch from mandibles of 14.5 d fetal mice and neonate 0 d under the stereoscope, and isolated dental cells primarily from the tissues. Then, we examined morphology and the gene expression profiles of the primary cells and dental tissues from fetus/neonate and adult with RT-PCR. Primary dental cells showed heterogeneous but the majority was shown as fibroblast-like morphology. The change of population doubling time levels (PDLs) showed that the primary dental cells have growth potential and could be expanded under our culture conditions without reduction of growth rate. Immunocytochemical and flow cytometric analyses were performed to characterize the primary dental cell populations from both of fetus (E14.5) and neonate. Alpha smooth muscle actin (${\alpha}-SMA$), vimentin, and von Willebrand factor showed strong expression, but desmin positive cells were not detected in the primary dental cells. Most of the markers were not uniformly expressed, but found in subsets of cells, indicating that the primary dental cell population is heterogeneous, and characteristics of the populations were changed during culture period. And mesenchymal stem cell markers were highly expressed. Gene expression profile showed Wnt family and its related signaling molecules, growth factors, transcription factors and tooth specific molecules were expressed both fetal and neonatal tissue. The tooth specific genes (enamelin, amelogenin, and DSPP) only expressed in neonate and adult stage. These expression patterns appeared same as primary fetal and neonatal cells. In this study we isolated primary cells from whole mandible of fetal and neonatal mice. And we investigated the characteristics of the primary cells and the profile of gene expressions, which are involved in epithelial-mesenchymal interactions during tooth development. Taken together, the primary dental cells in early passages or fetal and neonatal mandibles could be useful stem cell resources.

• 한국해안·해양공학회논문집
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• 제29권2호
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• pp.109-120
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• 2017

경사제 피복재를 예방적으로 유지관리할 수 있는 조건기반 할인비용모형을 제안하였다. 하중발생 사상을 이산시간 확률과정으로 고려하는 추계학적 누적 피해모형과 보수보강 비용에 대한 경제성 모형을 결합하여 수학적으로 유도하였다. 특히 본 논문에서 유도된 조건기반 유지관리의 할인비용모형은 시간에 따른 비용의 가치 뿐만 아니라 누적피해의 비선형성도 고려할 수 있다. 본 연구의 결과는 기존 모형들의 결과와 비교하여 만족스럽게 검증되었다. 또한 구조물의 중요도와 이자율 변화에 대한 민감도 분석도 수행하여, 구조물의 중요도가 높아질수록 예방적 보수보강의 최적시기는 빨라지나 이자율은 커질수록 반대의 경향이 나타난다는 것을 알았다. 한편 본 연구에서 유도된 추계학적 기대비용모형을 이용하여 여러 조건에 대하여 임의의 경사제 피복재 단면을 해석하였다. 표본경로기법을 적용하여 임의의 태풍 내습에 따른 경사제 피복재의 기대 누적피해수준을 예측하여 피해강도함수의 계수들을 추정할 수 있었다. 특히 하중발생 과정을 HPP(Homogeneous Poisson Process) 뿐만 아니라 DSPP(Doubly Stochastic Poisson Process)로도 해석하여 기대 누적피해수준에 미치는 하중발생의 불확실성에 대한 영향을 분석하여 하중발생사상을 이산시간 확률과정으로 고려해도 된다는 것을 확인하였다. 조건기반 할인비용모형의 해석 결과에 의하면 경사제 피복재의 설계조건에 따라 기대 누적피해수준의 거동특성이 크게 달라지고 이에 따라 예방적 보수보강을 수행하는 최적시기도 변한다는 것을 알 수 있었다. 마지막으로 파괴한계, 구조물의 중요도 그리고 이자율을 변화시키면서 예방적 유지관리를 가장 경제적으로 수행할 수 있는 최적시점과 피해규모를 결정할 수 있었다.