• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.21-25
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• 2008

Bone marrow mesenchymal stem cells (BMMSCs) have the capacity for self-renewal and differentiation into a variety of cell types. They represent an attractive source of cells for gene and cell therapy. The purpose of this study is to direct the specific expression of the DsRed reporter gene in $Sca-1^+$ BMMSCs differentiated into a cardiomyogenic lineage. We constructed the prMLC-2v-DsRed vector expressing DsRed under the control of the 309 tp fragment of the rat MLC-2v 5'-flanking region. The specific expression of the DsRed reporter gene under the transcriptional control of the 309 bp fragment of the rat MLC-2v promoter was tested in 5-azacytidine healed-$Sca-1^+$ BMMSCs over 2 weeks after the prMLC-2v-DsRed transfection. The prMLC-2v-DsRed was specifically expressed in the $Sca-1^+$ BMMSCs with cardiomyogenic lineage differentiation and it demonstrates that the 309 bp sequences of the rat MLC-2v 5'-flanking region is sufficient to confer cardiac specific expression on a DsRed reporter gene. The cardiac-specific promoter-driven reporter vector provides an important tool for the study of stem cell differentiation and cell replacement therapy in ischemic cardiomyopathy.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.4
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• pp.967-975
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• 1998

This paper presents a system to cancel the multi-user interference of synchronous DS/CDMA systems by using the estimation circuit and the reference correlator. In the code-division multiple access(CDMA) scheme, the spectrum of the user's signal is directly spreaded by means of the Simplex coed or the phase offsetted PN code. It is interesting for the Simplex code and the PN code to have a similar correalation property. It these codes are used in DS/CDMA systems, the number of acceptable users can be increased in comparison with conventioal DS/CDMA systems. This thesis presents a interference cancellation model to cancel the multi-use interference in DS/CDMA systems by using Simples code of PN code as a spreading sequence. In AWGN environment, we analyze interference generated from other users and compare the resulting performance with that of the conventioanl receiver in same user side.

• Parasites, Hosts and Diseases
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• v.58 no.6
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• pp.669-674
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• 2020

The diagnostic accuracy of dermoscopy (DS) for scabies, a highly contagious parasitic disease, remains disputed. This study aimed to assess the diagnostic accuracy of DS in scabies, analyze the factors influencing DS, and explore its role in post-treatment evaluation. Patients with suspected scabies were randomly divided into 2 groups: 71 patients in the skin scraping (SS) group and 73 patients in the DS group. The diagnostic efficiencies of SS and DS in these groups were calculated. We also analyzed the influence of body part and investigator competence on the accuracy of DS. Then 16 body parts with typical signs of scabies were monitored by DS 2 and 4 day after sulfur ointment treatment. The sensitivity and specificity of DS were 98.3% and 88.5%, respectively. Hands, arms, and the abdomen had higher positivity rates than other body parts (P<0.001). The accuracy of dermatologists' interpretations of images negative for scabies in the intermediate- and high-level groups was higher than that in the low-level group (P<0.001). At follow-up, the mites were still visible on 43.8% to 62.5% of the skin lesions 2 and 4 day after sulfur ointment treatment. These results showed that DS could significantly increase the accuracy of diagnosing scabies owing to its high sensitivity and specificity. Therefore, it may be useful for monitoring clinical responses to anti-parasitic treatment.

• Microbiology and Biotechnology Letters
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• v.52 no.2
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• pp.218-220
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• 2024

Bifidobacterium longum subsp. longum DS0950 (B. longum DS0950) was isolated from infant feces and has been reported to be effective in preventing obesity. The whole-genome sequence of B. longum DS0950 was obtained using the PacBio RS II platform, and it was consists of a single chromosome of 2,433,092 bp. The B. longum DS0950 contains genes associated with the synthesis of bacteriocins and a series of genes capable of producing xylitol from ribulose-5-phosphate.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.95-105
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• 2005

Transposon-mediated insertional mutagenesis provides one of the most powerful tools for functional studies of genes in higher plants. This project has been performed to develop a large population of insertional mutations, and to construct databases of molecular information on Ds insertion sites in rice. Ultimate goals are to supply genetic materials and information to analyze gene function and to identify and utilize agronomically important genes for breeding purpose. Two strategies have been employed to generate the large scale of transposon population in a Japonica type rice, Dongjin Byeo; 1) genetic crosses between Ac and Ds lines and 2) plant regeneration from seeds carrying Ac and Ds. Our study showed that over 70% of regenerated plants generally carried independent Ds elements and high activity of transposition was detected only during regeneration period. Ds-flanking DNA amplified from leaf tissues of F2 and T1 (or T2) plants have been amplified via TAIL-PCR and directly sequenced. So far, over 65,000 Ds lines have been generated and over 9,500 Ds loci have been mapped on chromosomes by sequence analysis. Database of molecular information on Ds insertion sites has been constructed, and has been opened to the public and will be updated soon at http://www.niab.go.kr. Detailed functional analysis of more than 30 rice mutants has been performed. Several Ds-tagged rice genes that have been selected for functional analysis will be briefly introduced. We expect that a great deal of information and genetic resources of Ds lines would be obtained during the course of this project, which will be shared with domestic and international rice researchers. In addition to the Japonica rice, we have established the tagging system in an rice line of indica genetic background, MGRI079. MGRI079 (Indica/Japonica) was transformed with Agrobacteria carrying Ac and Ds T-DNA vectors. Among transgenic lines, we successfully identified single-copy Ds and Ac lines in MGR1079. These lines were served as ‘starter lines’ to mutagenize Indica genetic background. To achieve rapid, large scale generation of Ds transposant lines, MGR1079 transformants carrying homozygous Ac were crossed with ones with homozygous Ds, and $F_2$seeds were used for plant regeneration. In this year, over 2,000 regeneration plants were grown in the field. We are able to evaluate the tagging efficiency in the Indica genetic background in the fall.

• Journal of the Korea Society of Computer and Information
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• v.15 no.6
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• pp.37-48
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• 2010

In general, an HTTP server sends information in response to requests from clients. Since it does not support active information delivery to clients, it can not efficiently provide the rapidly changing information to clients. Overcoming this shortcoming of the HTTP protocol, the technology known as server push enables the HTTP server to actively provide information to clients without explicit requests from clients. Adobe BlazeDS is a web server supporting the server push technology, helping users to develop web-based push applications. Unfortunately, since the BlaseDS server have no functions of filtering the information to be pushed according to various types of users, there are difficulties in developing push applications handling various situations in a efficient way. In this paper, to support effective development of push applications on BlazeDS we present the methods of adding a message filtering facility to BlazeDS and utilizing it. According to the filtering request of clients, the message filtering facility modifies information to be pushed, sending the modified information to the clients. The extended BlazeDS server with the message filtering facility provides environments to easily develop push services customized for various clients with their own situations.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.3
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• pp.355-362
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• 2003

In this paper, we analyzed the DS/CDMA System with Hybrid SC/MRC diversity, as a method to achieve a good performance by reducing the complexity of system, when PLL gain value was revised a phase gulf among branches to make perfect coherent receive signal. The channel is a multipath Nakagami-m fading channel, we consider user(K), processor gain(N), number of paths(Lp) in DS/CDMA system with hybrid SC/MRC diversity. We found that PLL gain values exist to make perfect coherent receive signal. We verified that DS/CDMA system with Hybrid SC/MRC diversity can improve performance when the proper PLL gain values is used in the system. As a result, we verified that the upper limit of PLL gain value is 5dB to make perfect coherent receive signal in the DS/CDMA system with hybrid SC/MRC diversity.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.40 no.8
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• pp.307-317
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• 2003

We analyze the performance of MC-CDMA and MC-DS/CDMA system on quasi-synchronized moble satellite return link. Quasi-synchronization is considered that chip offset between terminals is within a few chips. In 10-3 BER, performance of MC-DS/CDMA system with walsh code is 0.3dB better than that of MC-CDMA system when the number of user is 5 from -0.5 $T_{c}$ to 0.5 $T_{c}$ quasi-synchronization of MC-DS/CDMA system and from -5/64 $T_{c}$ $^{MC}$ to 5/64 $T_{c}$ $^{MC}$ quasi-synchronization of MC-CDMA system. MC-DS/CDMA system with walsh code is over 0.2dB better than that of MC-DS/CDMA system with extended m code and gold code. MC-CDMA system with walsh code is over 1.2dB better than that of MC-CDMA system with gold code when the number of user is 5 and lower than from -5/32 $T_{c}$ $^{MC}$ to 5/32 $T_{c}$ $^{MC}$ quasi-synchronization.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.39-45
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• 2000

Two maize transposable elements, immobilized Ac (iAc) and Ds, have been introduced into the genome of a diploid potato clone (Solanum tuberosum Group Phureja clone 1.22). The iAc is a modified Ac that is supposed to be unable to transpose but is expected to trans-activate the transposition of a Ds that is unable to transpose by itself. When the leaf and stem explants of in vitro shoots of the clone 1.22 were inoculated with Agrobacterium tumefaciens strains harboring binary vectors containing the iAc and the Ds, calli were formed from the explants on media containing 50 mg/L of kanamycin, and shoots were regenerated from the calli. The regenerated shoots formed roots when cultured on media containing 100 mg/L of kanamycin, whereas untransformed shoots did not form roots on the same media. The PCR amplification of the DNA's from the transgenic plants confirmed that the iAc and the Ds elements were introduced into the potato genome of 1.22.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.11
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• pp.53-60
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• 2005

In this paper, we analyze the performance of UWB MC-CDMA system and compareit with DS-CDMA and OFDM systems, which have been drafting in standardization even now. Too many Rake Fingers are needed in the DS-CDMA system to separate multi-path signals, which results in highsystem complexity. OFDM radio power fails to qualify for FCC certification unless frequency hopping. From this reason, MC-CDMA system considered is proposed in this paper. It has lower complexity compared with DS-CDMA and shows good performance against frequency selective fading. In addition, for a wide-band communication, less radio power per spectrum is allowed in the MC-CDMA system than in an OFDM system. Simulation result show that the DS-CDMA system has better performance with single user, but MC-CDMA system shows best performance in case of multi user environment.