• Korean Journal of Microbiology
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• v.43 no.4
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• pp.250-255
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• 2007

The RecA protein of Deinococcus radiodurans is essential for the extreme radiation resistance of this organism. The central steps involved in recombinational DNA repair require DNA-dependent ATP hydrolysis by recA protein. Key feature of RecA protein-mediated activities is the interactions with ssDNA and dsDNA. The ssDNA is the site where RecA protein filament formation nucleates and where initiation of DNA strand exchange takes place. The effect of sequence heterogeneity of ssDNA was examined in this experiment. The rate of homopolymeric synthetic ssDNA-dependent ATP hydrolysis was constant or nearly so over a broader range of pHs. For poly(dT)-dependent ATP or dATP hydrolysis, rates were generally faster, with a broader optimum between pH 7.0 and 8.0. Activities of RecA protein were affected by the ionic environment. The ATPase activity was shown to have different sensitivity to anionic species. The presence of glutamate seemed to slimulate the hydrolytic activity. Dr RecA protein was shown to require $Mg^{2+}$ ion greater than 2 mM for binding to etheno ssDNA and the binding stoichiometry of 3 nucleotide for RecA protein monomer.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.20 no.3
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• pp.129-139
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• 2009

Objectives : This study evaluated the effectiveness of school-based mental health screening and treatment linkage programs, focusing on attention-deficit hyperactivity disorder (ADHD) and adolescent depression. Methods : All 11,158 children and adolescents aged 8-14 years in Seoul received a school-based mental health screening, consisting of the Korean versions of the ADHD rating scale and the Child Behavior Checklist (CBCL), for ADHD, and the Center for Epidemiological Studies Depression Scale (CES-DS) and the Suicidal Ideation Questionnaire-Junior (SIQ-JR), for depression. The high-risk children's and adolescents' diagnoses were confirmed using the Diagnostic Interview Schedule for Children-IV (DISC-IV). Treatment linkage programs were managed by the Seoul Metropolitan Community Mental Health Center (Program 1), the Seoul Metropolitan Office of Education (Program 2), or the Dongjak District Office of Education (Program 3). We estimated and compared the referral rates of the three programs. Results : Program 1 screened and referred 22.9% of ADHD youths to mental health services, Program 2, 68.8%, and Program 3, 40.0%. Program 1 screened and referred 22.8% of depressed youths to mental health services, Program 2, 53.8%, and Program 3, 88.9%. Key elements for successful screening and referral programs were an effective school/community mental health center/Office of Education network, the parents' financial support and perception of their child's mental health status as being problematic, and the teachers' active engagement. Conclusion : This is the first study investigating the effectiveness of school-based mental health screenings' linkage to treatment for primary and middle school students in Korea. An effective network for community mental health and improvements in parents' and teachers' perceptions regarding mental health are needed for more successful treatment linkage.

• Journal of Environmental Health Sciences
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• v.37 no.5
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• pp.348-357
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• 2011

Objectives: This study was conducted in order to examine the relationship between heavy metal concentrations in the soil and the level of heavy metals in the blood or urine of 216 local residents living near abandoned metal mines. Methods: Residents around abandoned metal mines were interviewed about their dietary habits, including seafood consumption, medical history, cigarette smoking, and drug history. Metal concentrations in the soil were determined by atomic absorption spectrophotometer (AA-7000, Shimadzu, Japan). Lead (Pb) and cadmium (Cd) contents in the blood or urine were analyzed by GF-AAS (AA-6800, Shimadzu). Mercury (Hg) contents in the blood were determined by means of a mercury analyzer (SP-3DS, NIC). Arsenic (As) content in the soil and urine were measured by a HG-AAS (hydride vapor generation-atomic absorption spectrophotometer). Results: The heavy metal concentrations in the soil showed a log normal distribution and the geometric means of the four villages were 8.61 mg/kg for Pb, 0.19 mg/kg for Cd, 1.81 mg/kg for As and 0.035 mg/kg for Hg. The heavy metal levels of the 216 local residents showed a regular distribution for Pb, Cd, Hg in the blood and As in the urine. The arithmetic means were 3.37 ${\mu}g$/dl for Pb, 3.07 ${\mu}g$/l for Cd and 2.32 ${\mu}g$/l for Hg, 10.41 ${\mu}g$/l for As, respectively. Conclusions: As a result of multi-variate analysis for the affecting factors on the bodily heavy metal concentrations, gender and concentration in the soil (each, p<0.01) for blood lead levels; gender and smoking status (each, p<0.01) for blood cadmium levels; gender (p<0.01) for urine arsenic levels; gender, age and concentration in the soil (p<0.01) for blood mercury levels were shown to be the affecting factors.

• Tuberculosis and Respiratory Diseases
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• v.70 no.6
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• pp.498-503
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• 2011

Background: The causes of exertional desaturation in patients with COPD can be multifactorial. We aimed to investigate factors predict exertional desaturation in patients with moderate to severe COPD. Methods: We tested 51 consecutive patients with stable COPD (FEV1/FVC, $40{\pm}13%$ predicted). Patients performed a six minute walk test (6MWT). Pulse oxymetric saturation (SpO2) and pulse rate were recorded. Results: Oxygen desaturation was found in 15 subjects after 6MWT, while 36 subjects were not desaturated. Lung diffusing capacity was significantly lower in desaturation (DS) group ($62{\pm}18%$ predicted) compared with not desaturated (ND) group ($84{\pm}20$, p<0.01). However there was no statistical difference of FEV1/FVC ratio or residual volume between two groups. The pulse rate change was significantly higher in the desaturated compared with the not desaturated group. Six minute walking distance, subjective dyspnea scale, airflow obstruction, and residual volume did not predict exertional oxygen desaturation. Independent factors assessed by multiple logistic regression revealed that a pulse rate increment (odd ratio [OR], 1.19; 95% confidence interval [CI], 1.01~1.40; p=0.02), a decrease in baseline PaO2 (OR, 1.105; 95% CI, 1.003~1.218; p=0.04) and a decrease in lung diffusing capacity (OR, 1.10; 95% CI, 1.01~1.19; p=0.01) were significantly associated with oxygen desaturation. Receiver operator characteristic (ROC) analysis showed that an absolute increment in pulse rate of 16/min gave optimal discrimination between desaturated and not desaturated patients after 6MWT. Conclusion: Pulse rate increment and diffusion capacity can predict exertional oxygen desaturation in stable COPD patients with moderate to severe airflow obstruction.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.4A
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• pp.352-360
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• 2008

Recently Task Group (TG) 4 of the Institute of Electrical and Electronics Engineers (IEEE) 802.15a has been recommended a system with ranging capability in existence of multiple Simultaneous operating piconets (SOPs) as well as low-cost, low-power. According to the ranging service, coherent and non-coherent based ranging schemes using ternary code have been adopted as a standard. However it is hard to estimate an accurate time of arrival (TOA) in case of using direct sequence based TOA estimation method because pulse repetition interval (PRI) offered by TG is more limited than the maximum excess delay (MED) of channel. To mitigate inter pulse interference (IPI) problem, this paper proposes a non-coherent TOA estimation scheme using non-periodic transmission (NPT) pattern. The proposed receiver is based on a non-coherent energy detection considering with motivation of low rate wireless personal area network (LR-WPAN). TOA information is estimated via proper comparison with a prescribed threshold after the sliding correlation and search back window (SBW) process for reducing TOA error. To verify the performance of proposed ranging scheme, two distinct channel models approved by IEEE 802.15.4a TG are considered. According to the simulation results, we could conclude that the proposed scheme have performed better performance than the conventional method on the existence of multiple SOPs.

• Spatial Information Research
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• v.20 no.1
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• pp.9-18
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• 2012

South Korea has many landslides caused by heavy rains during summer time recently and the landslides continue to cause damages in many places. These landslides occur repeatedly each year, and the frequency of landslides is expected to increase in the coming future due to dramatic global climate change. In Korea, 81.5% of the population is living in urban areas and about 1,055 million people are living in Seoul. In 2011, the landslide that occurred in Seocho-dong killed 18 people and about 9% of Seoul's area is under the same land conditions as Seocho-dong. Even the size of landslide occurred in a city is small, but it is more likely to cause a big disaster because of a greater population density in the city. So far, the effort has been made to identify landslide vulnerability and causes, but now, the new dem and arises for the prediction study about the areal extent of disaster area in case of landslides. In this study, the diffusion model of the landslide disaster area was established based on Cellular Automata(CA) to analyze the physical diffusion forms of landslide. This study compared the accuracy with the Seocho-dong landslide case, which occurred in July 2011, applying the SCIDDICA model and the CAESAR model. The SCIDDICA model involves the following variables, such as the movement rules and the topographical obstacles, and the CAESAR model is also applied to this process to simulate the changes of deposition and erosion.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.55-61
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• 2008

Previous studies on genetic transformation of chrysanthemum using cold regulated gene (BN115) have been conducted and the PCR and Real-Time PCR based method to determine the presence of the transferred cold regulated gene in the chrysanthemum was established. To check whether over-expression of BN115 gene in transgenic chrysanthemum will enhance their tolerance to cold stress, the transgenic chrysanthemum were grown under low temperature condition and several cold signalling including growth characteristics, stoma size and shape, SPAD value and ion leakage test were investigated. The transgenic chrysanthemum in the low temperature growth chamber grow much faster in term of the height, number and size of the leaves than those of wild-type plants and damage of transgenic plant caused by the low temperature was much less than that of wild-type plants. The stoma type and size of transgenic plant leaves grown at $5^{\circ}C$ were much similar to of wild-type plant cultured on $25^{\circ}C$ It has been found that SPAD value of transgenic plants was much higher than those of wild-type, but the EC density being lower under low temperature condition.

• Therapeutic Science for Rehabilitation
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• v.4 no.2
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• pp.51-62
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• 2015

Objectives : This research was conducted to plan a strategy on the activities for ADHD who want to improve, and to use the strategy to find out how it influences the occupational performance when applying Cognitive Orientation to a daily Occupational Performance (CO-OP) approach. Methods : A single Subject AB design was employed. The subjects was one children with ADHD. The experiment consisted of 10 sessions spread over 7 weeks. The objective selected through COPM with guardians was improvement of chopstick use by children. In the course of the intervention, the overall CO-OP process was explained, continued analysis of performance and practice were encouraged in order to learn the skill of chopstick use, and guidance was given for DSS. Performance was measured by the time to move beans from one plate to another, starting from when the chopsticks were picked up. Results : The time to pick up the beans was 22.3s before the intervention, which was decreased by 7.2s to 15.1s after the intervention. The parents' COPM score showed an improvement of 3 points for performance and 4 points for satisfaction, demonstrating a clinically significant change. Conclusion : This study provides evidence that CO-OP is beneficial in occupation performance in children with ADHD.

• Proceedings of KOSOMES biannual meeting
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• 2007.11a
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• pp.185-185
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• 2007

Starting at the beginning q the 20th century, increasing amounts of tetrach1oroethene (PCE) and trichloroethene (TCE)were manufactured due to the extensive use of these compounds in industry, in the military, and in private households, mainly as nonflammable solvents. This widespread use, along with careless handling and storage, are among the most serious contaminants of soil, sediment and groundwater. Highly chlorinated ethenes are typically not degraded through oxygenation by aerobic bacteria Since complete reductive dechlorination of PCE and TCE to ethene (ETH) has been observed in anaerobic enrichment culture, anaerobic dehalorespiring bacteria have received increased attention in the last decade. Under anaerobic conditions, these compounds con be reductively dehalogenated to less-chlorinated ethenes or innocuous ethene by microorganism through dehalorespiration. We have been studying anaerobic enrichment culture which used lactate as the electron donor for reductive dechlorination of PCE to ETH the anaerobic mixed microbial culture was enriched from the sediment sample taken from site contaminated with PCE. PCE was consistently and completely converted to ethene. In addition, the accumulation of intermediate products such as 1,2-ds-dichloroethene (cis-DCE) and vinyl chloride (VC) was observed in the anaerobic mixed microbial culture. the established dechlorinating enrichment culture was analyzed by DGGE using primers specific to DefrJ1ococcoides 16S rRNA gene sequences. In conclusion, we established the PCE dechlorinating enrichment culture and confirmed the existence of Dehalococcoides in an enrichment culture.

• Journal of agricultural medicine and community health
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• v.43 no.3
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• pp.180-190
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• 2018

Objectives: The purpose of this study is to investigate the influencing factor of the quality of life of the elderly with mild dementia in rural community. Methods: A total of 123 elderly who were managed by public health center participated. They were diagnosed as mild dementia by MMSE-DS and CDR. Data were collected during the period from May to September in 2017 by a structured questionnaire that included general characteristics, depression, dependence, comorbidity, sleep scale. Data were analyzed by t-test, ANOVA, Pearson's correlation analysis, and multiple regression analysis. Results: The quality of life was correlated significantly with depression. Depression was the factor affecting the quality of life of the elderly with mild dementia in rural community. Depression accounted for 54.0% as a variable of quality of life. Conclusion: This study was meaningful in that it directly measured the quality of life of the elderly with mild dementia is known to be difficult to measure and found meaningful results. it is necessary to identify the cause of depression and establish a linkage system between public health centers and health related organizations in the community. We suggest the need for individualized preventive intervention program and integrated psychological health service considering depression.