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Detection of Mycoplasma gallisepticum using Polymerase Chain Reaction(PCR) (PCR 기법을 이용한 Mycoplasma gallisepticum의 검출)

  • Lee, Young-ju;Kim, Ki-seuk;Kim, Jong-wan;Tak, Ryun-bin
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.90-95
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    • 1999
  • A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum(M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.

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GML Map Visualization on Mobile Devices

  • Song, Eun-Ha;Jeong, Young-Sik
    • Journal of Information Processing Systems
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    • v.6 no.1
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    • pp.33-42
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    • 2010
  • GIS can only be applied to certain areas by storing format. It is subordinate to a system when displaying geographic information data. It is therefore inevitable for GIS to use GML that supports efficient usage of various geographic information data and interoperability for integration and sharing. The paper constructs VisualGML that translates currently-used geographic information such as DXF (Drawing Exchange Format), DWG (DraWinG), or SHP (Shapefile) into GML format for visualization. VisualGML constructs an integrated map pre-process module, which filters geographic information data according to its tag and properties, to provide the flexibility of a mobile device. VisualGML also provides two major GIS services for the user and administrator. It can enable visualizing location search. This is applied with a 3-Layer POI structure for the user. It has trace monitoring visualization through moving information of mobile devices for the administrator.

Game Theoretic based Distributed Dynamic Power Allocation in Irregular Geometry Multicellular Network

  • Safdar, Hashim;Ullah, Rahat;Khalid, Zubair
    • International Journal of Computer Science & Network Security
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    • v.22 no.7
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    • pp.199-205
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    • 2022
  • The extensive growth in data rate demand by the smart gadgets and mobile broadband application services in wireless cellular networks. To achieve higher data rate demand which leads to aggressive frequency reuse to improve network capacity at the price of Inter Cell Interference (ICI). Fractional Frequency Reuse (FFR) has been recognized as an effective scheme to get a higher data rate and mitigate ICI for perfect geometry network scenarios. In, an irregular geometric multicellular network, ICI mitigation is a challenging issue. The purpose of this paper is to develop distributed dynamic power allocation scheme for FFR based on game theory to mitigate ICI. In the proposed scheme, each cell region in an irregular multicellular scenario adopts a self-less behavior instead of selfish behavior to improve the overall utility function. This proposed scheme improves the overall data rate and mitigates ICI.

Patterns of Spontaneous Adverse Events Reporting on Human Papillomavirus Vaccines according to the Applicability of Brighton Collaboration Criteria in Korea from 2008 to 2017 (국내 사람유두종바이러스백신 접종 후 자발적 이상반응 보고사례의 Brighton Collaboration 기준 활용 가능성 연구)

  • Kim, Myo-Song;You, Seung-Hun;Park, Hye Min;Lee, Min-Taek;Kang, Ye-Jin;Koo, Hyunji;Jung, Sun-Young
    • Korean Journal of Clinical Pharmacy
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    • v.30 no.1
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    • pp.19-30
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    • 2020
  • Objective: To describe patterns of spontaneous reporting on adverse events following immunization (AEFIs) using the human papilloma virus (HPV) vaccine according to the Brighton Collaboration (BC) criteria. Methods: We used the Korea Adverse Event Reporting System (KAERS) database including vaccinations between 2008 and 2017. To apply BC criteria, we classified 58 BC AEFIs into World Health Organization Adverse Reaction Terminology (WHO-ART) codes. We applied MedDRA standard medical queries that were pre-defined as five BC AEFIs. Terminology mapping between MedDRA and WHO-ART terms was performed by three researchers. Descriptive statistics of individual case safety reports were analyzed according to BC applicability. Disproportionality analyses were performed on each BC AEFI and each preferred AEFI term according to the case-noncase approach; reporting odds ratio (ROR) and 95% confidence intervals (CI) were calculated. Results: Among the 30,266 reports of vaccinations between 2008 and 2017, 2,845 reports included the HPV vaccine. Of these reports, 1,511 (53.1%) included at least one BC AEFI. Reports from physicians or manufacturers included more BC AEFIs than from other reporters. Injection site reactions and fever were frequently reported in BC AEFIs; spontaneous abortion and ectopic pregnancy (ROR, 14.29 [95% CI, 4.30-47.49]) and vasculitic peripheral neuropathy (ROR, 8.57 [95% CI, 2.61-28.10]) showed the highest ROR. Among non-BC AEFIs, dizziness or myalgia were frequently reported; exposure during pregnancy (ROR, 23.95 [95% CI, 16.27-35.25]) and inappropriate schedule of administration (ROR, 22.89 [95% CI, 16.74-31.31]) showed the highest ROR. Conclusion: BC criteria would be applicable for labeled AEFIs, whereas analyzing non-BC AEFIs would be useful for detecting unlabeled AEFIs.

Variation of Mitochondrial DNA Restriction Fragments of Common Rats, Rattus norvegicus caraco Pallas (Mammalia , Redentia) , from Cheongju , Korea (청주에 서식하는집쥐[Rattus norvegicus caraco Pallas(설치목, 포유강)]의 미토콘드리아 DNA 절단단편의 변이)

  • Hung Sun Koh;Yong Seok Roh;Sang Bok Kim;Byung Sun Yoo
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.4
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    • pp.409-416
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    • 1995
  • Forty samples of common rats (Rattus norvegicus caraco) from Cheongu, Korea, were used for the analyses of mitochondrial DNA (mtDNA) fragment patterns resulted from the digestion with eight restriction enzymes. A total of 36 fragments were recognized and six mtDNA clones were revealed . The nucleotide-sequence divergences (p) among six mtDNA clones ranged from 0.35% to 2.73%. moreover, the six clones were grouped into three major subgroups ; the first, second , and third subgroup were composed of 29 samples of three clones, ten samples of two clones, and one sample of one clone, respectively. The second and third subgroups were different in their mtDNa genotype of Pvu II from the first subgroup, and the third subgroup differed in the genotype of Dra I from other two subgroups. Futhermore, the maximum divergence among common rats from Korea in this study is greater than that among common rats from the United States and Japan by Brown and Simpson (1981). Further analyses with additional sample from other localities in Korea appeared to be necessary in order to clarify the taxnomic status of the distinct mtDNA subgroups.

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Development of a Period Analysis Algorithm for Detecting Variable Stars in Time-Series Observational Data

  • Kim, Dong-Heun;Kim, Yonggi;Yoon, Joh-Na;Im, Hong-Seo
    • Journal of Astronomy and Space Sciences
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    • v.36 no.4
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    • pp.283-292
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    • 2019
  • The purpose of this study was to develop a period analysis algorithm for detecting new variable stars in the time-series data observed by charge coupled device (CCD). We used the data from a variable star monitoring program of the CBNUO. The R filter data of some magnetic cataclysmic variables observed for more than 20 days were chosen to achieve good statistical results. World Coordinate System (WCS) Tools was used to correct the rotation of the observed images and assign the same IDs to the stars included in the analyzed areas. The developed algorithm was applied to the data of DO Dra, TT Ari, RXSJ1803, and MU Cam. In these fields, we found 13 variable stars, five of which were new variable stars not previously reported. Our period analysis algorithm were tested in the case of observation data mixed with various fields of view because the observations were carried with 2K CCD as well as 4K CCD at the CBNUO. Our results show that variable stars can be detected using our algorithm even with observational data for which the field of view has changed. Our algorithm is useful to detect new variable stars and analyze them based on existing time-series data. The developed algorithm can play an important role as a recycling technique for used data

The DNA region of rtn gene essential for resistance against N4 infection (N4에 대해 내성을 나타내는데 필요한 rtn 유전자의 부위)

  • 이동환;유선미;황의욱;이영훈;채건상
    • Korean Journal of Microbiology
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    • v.29 no.5
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    • pp.290-295
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    • 1991
  • N4 phage, which infects E. coli K-12 strains, could not infect E. coli K-12 strains containing rtn(resistant to N4) gene on plasmids, which was isolated from Proteus vulgaris ATCC 13315. The region of rtn gene for Rtn phenotype was reduced to the 1.7 kb HincII-AccI fragment, and rtn gene seemed to have its own promoter. This putative promoter was present in 107 bp HindII-DraI fragment, and known to be functional in E. cole K-12, which is supported by the fact that phenotype of a subclone, pRMG103A1B which does not contain the 107 bp fragment, was dependent on the existance of a functional promoter in the upstream of rtn gene, and that the 107 bp fragment had promoter activity when located in the upstream of structural gene of galactodinase of E. coli. The promoter-bearing fragment contains two overlapping putative promoter sequences, both of which show a fit in eight of twelve nucleotides with consensus sequences of E. coli promoters at the -35 and -10 regions.

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SPECTRA OF CHROMOSPHERICALLY ACTIVE STARS (채층 활동이 강한 별들의 분광선)

  • KANG YOUNG WOON;KIM HOIL;LEE WOO BAIK;OH KYU DONG
    • Publications of The Korean Astronomical Society
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    • v.15 no.spc1
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    • pp.93-102
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    • 2000
  • We have reviewed the magnetic activity in close binaries. Solar like magnetic activity indicators such as photometric spots, chromo spheric emission, coronal X-ray and radio emission, and flare activity are commonplace in many cool stars with convective envelopes. Using the UV spectra we confirmed the strength of stellar activity increases with more rapid rotation and later spectral types which corresponds to the increasing depth of the star's convective envelope. Apart from very young stellar objects such as T Tauri stars, the stars with the highest levels of activity are close binary systems composed of cool stars, i.e., the chromospherically active binaries such as RS CVn, BY Dra, W UMa and related systems. The IUE low and high dispersion spectra of V711 Tau, VW Cep and SW Lac are used for ultraviolet photometry and for a variation study of chromospheric activity. Evidence of chromospherically activity is indicated by the intensity variation of the Mg II emission line with orbital phase.

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Polymorphisms of Cytochrome P450 2E1 Gene in Korean Patients with Renal Failure

  • Yoo, Min
    • Biomedical Science Letters
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    • v.19 no.4
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    • pp.310-314
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    • 2013
  • CYP2E1 in the liver has been studied intensively because it is involved in the metabolic activation of xenobiotics. It is inducible by alcohol, so it has been suspected as the cause of cancer in the stomach and lung. The possible role of CYP2E1 has been suggested strongly as causing tissue damage in mice with renal failure. It was also suspected that 5'-flanking region of CYP2E1 gene might be involved with renal failure. So, we investigated polymorphism of restriction enzyme sites within CYP2E1 gene using the PCR-RFLP analysis. PstI and RsaI sites were located at 5'-flanking region and DraI site was located at intron 6. All three types (W/W, W/S, S/S) were observed for these enzymes although each incidence was somewhat different depending the enzyme sites. W/W was prominent for PstI whereas W/S was markedly high for RsaI. Overall, polymorphic incidence in patients was somewhat higher than normal population. This research should facilitate further investigation of CYP2E1 at genetic level as the direct cause of tissue damage in various organs.

MOEPE: Merged Odd-Even PE Architecture for Stereo Matching Hardware (MOEPE: 스테레오 정합 하드웨어를 위한 Merged Odd-Even PE구조)

  • Han, Phil-Woo;Yang, Yeong-Yil
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.10
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    • pp.57-64
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    • 2000
  • In this paper, we propose the new hardware architecture which implements the stereo matching algorithm using the dynamprogrammethod. The proposed MOEPE(Merged Odd-Even PE) architecture operates in the systolic manner and finds the disparities form the intensities of the pixels on the epipolar line. The number of PEs used in the MOEPE architecture is the same number of the range constraint, which reduced the nuMber of the necessary PEs draMatically compared to the traditional method which uses the PEs with the same number of pixels on the epipolar line. For the normal sized images, the numof the MOEPE architecture is less than that of the PEs in the traditional method by 25${\times}$The proposed architecture is modeled with the VHDL code and simulated by the SYNOPSYS tool.

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