• 한국축산시설환경학회지
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• 제14권1호
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• pp.9-14
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• 2008

본 시험은 두당 동일한 면적에서 비육전기 한우 거세우의 계절별 개체유지 및 사회적 행동 특성을 구명하고자 실시하였다. 15개월령의 한우 거세우 48두를 공시하여 두당 사육면적은 동일한 상태에서 각각 4두, 8두, 12두씩 2반복으로 시험을 수행하였으며, 행동측정은 15개월령, 17개월령, 19개월령에 분석하였으며, 그 결과를 요약하면 다음과 같다. 1. 섭식행동(FD) 중 섭취시간은 가을철에 가장 높게 나타났으며, 여름철이 가을, 겨울철 보다 유의적으로 짧은 섭취시간을 나타냈다(p<0.05). 2. 기립행동(ST)은 여름철이 유의적으로 가장 짧은 시간을 보였다(p<0.05). 3. 횡와행동(LD)은 여름철이 가을, 겨울철보다 유의적인 증가를 나타냈다(p<0.05). 4. 음수행동(DR)에서는 겨울철에 유의적으로 감소하였다(p<0.05). 5. 개체유지 행동 중 함기행동(SG)과 긁기행동(SR)은 여름철보다 겨울철에 유의적인 증가를 보였다(p<0.05). 6. 사육공간별 행동 특성에서는 마리당 평균시간에서 걷기행동(WA)이 군 두수가 가장 큰 그룹에서 유의적(p<0.05)으로 증가하였으며, 마리당 평균빈도에서는 군 두수가 가장 작은 그룹에서 자기몸핥기(SG)가 유의적(p<0.05)으로 증가하였다. 이상의 결과를 토대로 계절 및 군 두수별로 한우 거세우의 행동 특성이 각각 다른 것으로 나타났고, 향후 보다 더 많은 개체수를 대상으로 다양한 시험조건 하에서 시험을 수행한다면 시설 및 환경을 연계해 친환경 축사조건을 구명할 수 있을 것으로 판단된다.

• 한국임상수의학회지
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• 제22권4호
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• pp.386-391
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• 2005

This study was done to produce a mutated protein inactivated cytotoxicity of Shigatoxin 2e (Stx2e) of E.coli O139 isolates by deletional mutagenesis of Stx2e A subunit gene encoding active-site cleft of enzymatic domain in ST2e holotoxin. Cytotoxicity of the toxoid expressed from the mutant Stx2e gene was compared with wild type Stx2e for development of vaccine candidate. A recombinant plasmid pED18 containing Stx2e gene ot E.coli O139 isolates was used to generate mutation plasmid. Deletion mutagenesis was conducted for Stx2e A subunit gene encoding enzymatically active domain by polymerase chain reaction (PCR) using ot designed primer to induce deletional mutation. DNA sequence analysis was confirmed that the pentamer (Typ 202- Ser 206) that lies within the proposed active-site cleft in the second region was completely deleted. A DNA fragment of 1.1 kb that encode the new mutant Stx2eA gene was inserted into plasmid pRSET vector digested with EcoRV-Hind III and named pEDSET The PEDSET was transformed in E. coli for expression of mutant protein and the protein was confirmed by SDS-PACE and Western-blotting. The protein expressed by the mutant was tested to confirm the reduction of cytotoxic activities on Vero cell using microcytotoxicity assay compared with wild type Stx2e, the cytotoxicity of deletional mutant protein was at least reduced by 3,000-fold on Vero cell.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제28권4호
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• pp.213-219
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• 2017

Objectives: Early intensive interventions are very important for children with autism spectrum disorder. We examined the actual conditions of hospital-based early intensive interventions for autism spectrum disorder in Seoul, in order to help develop and implement an evidence-based early intensive intervention model for use in Korea. Methods: Nine hospital-based institutes running an early intensive intervention program for children with autism spectrum disorder responded to a questionnaire in September 2014. They provided a brief introduction to their program, explained its theoretical bases, and reported the number of children, their age, intervention time, duration and so on. Results: In the majority of the institutions, the intervention was provided for over 20 hours every week, and the theoretical bases included various applied behavioral analysis (ABA) methods and other therapies (language and occupational therapy). The therapist-child ratio ranged from 1:1 to 5:3. Various types of therapists were involved, including behavioral analysts, special education teachers and (or) language pathologists. There was only one clinic where the behavioral analyst was the main therapist. Usually, the intervention was terminated just before the child entered elementary school. The main merit of the hospital-based intervention in our survey was the effectiveness of the multi-disciplinary intervention plan and its other merits were the accuracy of the diagnosis, its ability to be combined with medicine, and so on. Conclusion: The current hospital-based early intensive intervention programs provide interventions for over 20 hours per week and employ multidisciplinary approaches. However, there are very few institutes for children with autism and very few intervention specialists and specialist education courses in the country. We need more educational programs for intervention therapists and have to try to develop policies which encourage the implementation of an evidence-based early intensive intervention program nationwide.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8947-8950
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• 2014

Immunological functions of heat shock proteins (HSPs) have long been recognized. In this study we aimed to efficiently purify HSP70 from renal cell carcinoma and test it as a tumor antigen for pulsing dendritic cells in vitro. HSP70 was purified from renal cell carcinoma specimens by serial column chromatography on Con A-sepharose, PD-10, ADP-agarose and DEAE-cellulose, and finally subjected to fast protein liquid chromatography (FPLC). Dendritic cells derived from the adherent fraction of peripheral blood mononuclear cells were cultured in the presence of IL-4 and GM-CSF and exposed to tumor HSP70. After 24 hours, dendritic cells were phenotypically characterized by flow cytometry. T cells obtained from the non-adherent fraction of peripheral blood mononuclear cells were then co-cultured with HSP70-pulsed dendritic cells and after 3 days T cell cytotoxicity towards primary cultured renal cell carcinoma cells was examined by Cell Counting Kit-8 assay. Dendritic cells pulsed in vitro with tumor-derived HSP70 expressed higher levels of CD83, CD80, CD86 and HLA-DR maturation markers than those pulsed with tumor cell lysate and comparable to that of dendritic cells pulsed with tumor cell lysate plus TNF-${\alpha}$. Concomitantly, cytotoxic T-lymphocytes induced by HSP70-pulsed dendritic cells presented the highest cytotoxic activity. There were no significant differences when using homologous or autologous HSP70 as the tumor antigen. HSP70 can be efficiently purified by chromatography and induces in vitro dendritic cell maturation in the absence of TNF-${\alpha}$. Conspecific HSP70 may effectively be used as a tumor antigen to pulse dendritic cells in vitro.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4193-4198
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• 2015

Background: Globally, retinoblastoma is the most common primary intraocular malignancy occurring in children. This paper documents the recent incidence rates of retinoblastoma by age and sex groups from the Population Based Cancer Registries (PBCRs) of Bangalore, Mumbai, Chennai, Delhi and Kolkata using the data from the National Cancer Registry Programme. Materials and Methods: Relative proportions, sex ratio, method of diagnosis, and incidence rates (crude and age standardized) for each PBCR and pooled rates of the five PBCRs were calculated for the years 2005/06 to 2009/10. Standard errors and 95% confidence limits of ASIRs by sex group in each PBCR were calculated using the Poisson distribution. Standardised rate ratios of ASIR by sex group and rate ratios at risk were also calculated. Results: The maximum retinoblastoma cases were in the 0-4 age group, accounting for 78% (females) and 81% (males) of pooled cases from five PBCRs. The pooled crude incidence rate in the 0-14 age group was 3.5 and the pooled ASIR was 4.4 per million. The pooled ASIR in the 0-4, 5-9 and 10-14 age group were 9.6, 2.0 and 0.1 respectively. The M/F ratio in Chennai (1.9) and Bangalore PBCRs (2.0) was much higher than the other PBCRs. Among the PBCRs, the highest incidence rate in 0-4 age group was found in males in Chennai (21.7 per million), and females in Kolkata (18.9 per million). There was a distinct variation in incidence rates in the PBCRs in different geographic regions of India.

• 한국멀티미디어학회:학술대회논문집
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• 한국멀티미디어학회 2000년도 추계학술발표논문집
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• pp.66-69
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• 2000

웹이 등장하면서 지금까지 인터넷 상에서 텍스트와 이미지를 이용하여 정보를 표현하고 전달하는 방법이 가장 많이 사용되어왔다. 그러나 웹 관련 기술의 비약적인 발달과 네트워크 속도의 증가 및 인터넷의 급속한 보급으로 단순한 텍스트와 이미지 중심의 HTML 문서를 이용한 정보의 전달이 아닌 멀티미디어 데이터를 이용한 정보의 표현과 전달이 점차 증대되고 있다. 이에 따라 멀티미디어 데이터를 전송하기 위한 스트리밍 프로토콜도 등장하였다. 최근에는 컴퓨터의 성능 증가 및 네트워크 속도의 증가(초고속 통신 서비스의 보급)에 의해 멀티미디어 데이터의 전송이 가능하게 됨으로써 기존의 공중파나 CATV 방송국의 형태 지니고 인터넷 상에서 실시간 생방송 서비스와 VOD(Video On Demand) 서비스를 제공하는 인터넷 방송국이 급속하게 생겨나고 있다. (11) 인터넷 방송은 동영상과 오디오의 실시간 전달을 가능하게 하는 멀티미디어 스트리밍 기술과 멀티미디어를 실시 간으로 전송할 수 있는 실시간 전송 프로토콜을 기반으로 발전하고 있다. 인터넷 상에서 멀티미디어 스트리밍 서비스를 하는 대부분의 인터넷 방송은 스트리밍 서버로서 RealNetworks사의 RealSystem과 Microsoft사의 WMT(Windows Media Technologies)를 사용하고 있다. 본 논문은 Real Server와 WMT의 비교 분석을 통해 실시간 전송 프로토콜을 지원하고, 멀티미디어 스트리밍 기술을 지원하는 자바를 기반으로 한 분산 서버 구조의 스트리밍 서버, 서버간의 부하를 제어하는 미들웨어, 멀티미디어 스트림을 재생할 수 있는 클라이언트를 설계하고 구현한다.있다.구현한다. 이렇게 구현된 시스템은 전자 상거래, 가상 쇼핑몰, 가상 전시화, 또는 3차원 게임이나 가상교육 시스템과 같은 웹기반 응용프로그램에 사용될 수 있다.물을 보존·관리하는 것이 필요하다. 이는 도서관의 기능만으로는 감당하기 어렵기 때문에 대학정보화의 센터로서의 도서관과 공공기록물 전문 담당자로서의 대학아카이브즈가 함께 하여 대학의 공식적인 직무 관련 업무를 원활하게 지원하고, 그럼으로써 양 기관의 위상을 높이는 상승효과를 낼 수 있다.하여는, 인쇄된 일차적 정보자료의 검색방법등을 개선하고, 나아가서는 법령과 판례정보를 위한 효율적인 시스템을 구축하며, 뿐만 아니라 이용자의 요구에 충분히 대처할 수 잇는 도서관으로 변화되는 것이다. 이와 함께 가장 중요한 것은 법과대학과 사법연수원에서 법학 연구방법에 관한 강좌를 개설하여 각종 법률정보원의 활용 내지 도서관 이용방법에 관하여 교육하는 것이다.글을 연구하고, 그 결과에 의존하여서 우리의 실제의 생활에 사용하는 $\boxDr$한국어사전$\boxUl$등을 만드는 과정에서, 어떤 의미에서 실험되었다고 말할 수가 있는 언어과학의 연구의 결과에 의존하여서 수행되는 철학적인 작업이다. 여기에서는 하나의 철학적인 연구의 시작으로 받아들여지는 이 의미분석의 문제를 반성하여 본다. 것이 필요하다고 사료된다.크기에 의존하며, 또한 이러한 영향은 $(Ti_{1-x}AI_{x})N$ 피막에 존재하는 AI의 함량이 높고, 초기에 증착된 막의 업자 크기가 작을 수록 클 것으로 여겨진다. 그리고 환경의 의미의 차이에 따라 경관의 미학적 평가가 달라진 것으로

• Maxillofacial Plastic and Reconstructive Surgery
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• 제34권1호
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• pp.26-33
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• 2012

Purpose: The golden ratio has been used for a long time to objectify and quantify 'beauty'. Dr. Marqurardt claims that the golden ratio can be applied in the maxillofacial field as well. The purpose of this study was to evaluate the diagnostic significance of using a facial 'phi' mask for analyzing Korean faces with characteristics of Class I, II, and III malocclusion. Methods: We studied twenty five Korean celebrities' frontal facial photos (10 males, 15 females) and 90 malocclusion patients' frontal facial photos (30 patients in each malocclusion classification: Class I, Class II, and Class III). Patients who received orthodontic treatment at Samsung Medical Center were selected for this study. After superimposition of the selected facial photo and facial 'phi' mask using Adobe Photoshop CS3, the ratio of the entire facial area, mid facial area, lower facial area and horizontal and vertical lengths were measured. Results: The facial ratio in photos of Korean faces showed larger vertical and horizontal ratios than the facial 'phi' mask with golden ratio, regardless of skeletal malocclusion (entire face: 115%, lower face: 125% larger than the mask). The results of the frontal photos of Class I, II, and III malocclusion patients using facial 'phi' mask showed that the vertical length and frontal face area was more significantly influenced by the area of the lower face than the midface. This means that the lower face has larger proportions in the facial areas. Conclusion: The ratio of facial 'phi' mask is matched with the ideal facial appearance that the contemporary Korean general public is seeking. Thus, the facial 'phi' mask may be a convenient tool for esthetic analysis of Korean faces. Reducing the area of the lower face is esthetically more desirable for almost all Korean people when planning orthognathic surgery.

• 대한치과교정학회지
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• 제23권2호
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• pp.217-227
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• 1993

치아이동시 생역학을 고려한 frictionless system이 공간폐쇄를 위해 이상적이지만, 이는 숙달된 wire bending이 필요하며 잘못 계산된 교정장치를 사용하였을때 부작용이 매우 클 수 있다. 최근 Straight Wire Appliance가 도입 됨에 따라 복잡한 wire bending없이 "Elastic tie back"을 이용한 공간폐쇄가 가능하지만 alignment와 leveling을 위한 치료기간이 길고 friction과 binding으로 인해 이상적인 교정력을 적용시키는데 어려움이 있다. 그러나 교정환자 수가 많아지고 짧은 치료 기간이 요구되어지는 요즈음에 선택적으로 "Elastic tie back"을 이용하는 공간폐쇄는 부작용을 최소화 하면서 손쉽게 이용할 수 있는 교정장치라고 사료된다.

• 한국발생생물학회지:발생과생식
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• 제20권4호
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• pp.331-347
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• 2016

Sexual dimorphism is the most conspicuous difference between the sexes. This study examines possible sexual dimorphism and the relative growth patterns of morphometric characteristics in the marine medaka, Oryzias dancena for their potential to help differentiate between males and females of this species. The von Bertalanffy growth parameters estimated by a non-linear regression method were $L_{\infty}=30.2mm$, K=3.22/year, and ${\tau}_0=-0.05$. All 18 characteristics measured showed a difference between males and females from 70 days after hatching. Each of these characteristics were significantly different between sexes (ANCOVA, P<0.05), and the ratio of standard length between sexes showed that males were larger than females for all five morphometric measurements. Fin length measurements were taken for 21 distances of anal fin and 7 distances of dorsal fin between landmarks. There were all differences for all dorsal fin rays between the males and the females and there is significant difference in 70 days after their hatch when the sexual dimorphism is presented. The significant difference (P<0.05) in fin ray for male and female was more greatly seen as they grow. Male marine medaka showed more rapid growth than females, with longer length, dorsal fins and anal fins. Differences in these characteristics will be useful during experiments when it is necessary to differentiate between sexes of marine medaka.

• 한국발생생물학회지:발생과생식
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• 제22권2호
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• pp.143-153
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• 2018

The large extracellular domain of glycoprotein hormone receptors is a unique feature within the G protein-coupled receptors (GPCRs) family. After interaction with the hormone, the receptor becomes coupled to Gs, which, in turn stimulates adenylyl cyclase and the production of cAMP. Potential phosphorylation sites exist in the C-terminal region of GPCRs. The experiments described herein represent attempts to determine the functions of the eel follicle-stimulating hormone receptor (eelFSHR). We constructed a mutant of eelFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 614 (eelFSHR-t614). The eelFSHR-t614 lacked all potential phosphorylation sites present in the C-terminal region of eelFSHR. In order to obtain the eelFSHR ligand, we produced recombinant follicle-stimulating hormone ($rec-eelFSH{\beta}/{\alpha}$) in the CHO-suspension cells. The expression level was 2-3 times higher than that of the transient expression of eelFSH in attached CHO-K1 cells. The molecular weight of the $rec-eelFSH{\beta}/{\alpha}$ protein was identified to be approximately 34 kDa. The cells expressing eelFSHR-t614 showed an increase in agonist-induced cAMP responsiveness. The maximal cAMP responses of cells expressing eelFSHR-t614 were lower than those of cells expressing eelFSHR-wild type (eelFSHR-WT). The $EC_{50}$ following C-terminal deletion in CHO-K1 cells was approximately 60.4% of that of eelFSHR-WT. The maximal response in eelFSHR-t614 cells was also drastically lower than that of eelFSHR-WT. We also found similar results in PathHunter Parental cells expressing ${\beta}$-arrestin. Thus, these data provide evidence that the truncation of the C-terminal cytoplasmic tail phosphorylation sites in the eelFSHR greatly decreased cAMP responsiveness and maximal response in both CHO-K1 cells and Path-Hunter Parental cells expressing ${\beta}$-arrestin.