• Natural Product Sciences
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• 제7권1호
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• pp.1-4
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• 2001

The antioxidant activity of Pinus densiflora was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The methanol extract of P. densiflora showed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate>n-butanol>water>dichloromethane> n-hexane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further purified by repeated silica gel column chromatography. Antioxidant (+)-catechin was isolated as one of the active principles from the ethyl acetate fraction, together with the inactive components, dihydrokaempferol and 1-O-benzoylglucose. The radical scavenging effect of (+)-catechin on DPPH radical exceeded to that of L-ascorbic acid which is a well known antioxidant.

• 한국식품영양과학회지
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• 제37권9호
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• pp.1095-1100
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• 2008

국내산 밤 일부 품종(단택, 대보, 석추, 옥광, 병고)의 기능성 성분 분석과 이들 물질들의 항산화력 및 밤 추출물이 대식세포 활성에 미치는 영향력을 알아보았다. 국내산 밤 일부 품종의 내피와 과육에서 coumarin, gallic acid 그리고 catechin이 HPLC에 의해 검출되었다. 밤 내피에 가장 풍부한 기능성 성분은 catechin이고 그 다음 gallic acid와 coumarin 순이었으나, 과육에서는 gallic acid만 검출되었다. 따라서 밤의 기능성 성분인 gallic acid와 catechin의 항산화력을 DPPH radical 소거작용과 SOD 유사활성을 통해 알아보았다. Gallic acid과 catechin을 6.0 mg/100 g 처리하였을 때 DPPH radical 소거작용이 각각 69.4%와 38.3%였으며, gallic acid의 DPPH radical 소거작용은 농도 의존적이었다. Gallic acid를 동일한 농도의 catechin과 비교하면 모든 농도 구간에서 catechin보다 gallic acid가 높은 DPPH radical 소거활성을 나타내었다. 그러나 SOD 유사활성은 gallic acid보다 catechin이 높았다. 더하여 밤의 과육, 노란과육 및 흰과육 추출물들이 대식세포 활성에 미치는 영향력을 알아보았다. 병고 흰과육 추출물은 현저하게 대식세포 활성을 증가시켰고, 병고, 단택, 대보 및 옥광의 노란과육 추출물 역시 아무 것도 처리하지 않은 세포와 비교하여 현저히 대식세포를 활성화시켰다. 노란과육 추출물이 흰과육 추출물보다 더욱 더 대식세포 활성을 증가시켰고, 5품종 중 병고가 대식세포 활성을 가장 증가시켰다.

• 동의생리병리학회지
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• 제21권5호
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• pp.1135-1141
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• 2007

In recent year, We are concerned in anti-aging, disease-protection, long-life, many method are used in solving this problem. Recently, We heard that Luffae Fructus Retinervus(LFR) has effect of anti-aging, disease-protection, long-life. So I let made a experiment for this result. The purpose of this study is to; 1) the anti-oxidant effect of Luffae Fructus Retinervus(LFR) used for 3 methods, those are DPPH radical scavenging activity, Nitric oxide(NO) radical scavenging activity, Superoxide anion radical scavenging activity, 2) cultivation 3T3-L 1 Preadipocytes and Protein chip used for ProteoPlexTM 16-Well Murine Cytokine Array Kit. We measured level of DPPH radical scavenging activity. And we experienced that the ability of DPPH radical's elimination was increased by rising concentration of LFR. When the concentration of LFR was 5 mg/ml, the ability of DPPH radical's elimination was Maximum. We measured level of Nitric oxide(NO) radical scavenging activity. And we founded that the ability of NO radical's elimination was significant when concentration of LFR was from 1.25 mg/ml to 2.5 mg/ml. We measured level of Superoxide anion radical scavenging activity. And we founded that the ability of Superoxide anion radical's elimination was maximum when concentration of LFR was 0.3125 mg. When we inspected Antioxidative Effects with BSA, we experienced that ability of defense was increased by rising concentration of LFR. We known the immunity of LFR about 3T3-L1 Preadipocytes and gained the increase of Cytokines(IL-2, IL-4, GM-CSF) without IL-12p70, $INF-{\gamma}$, $TNF-{\alpha}$ So I guess that Luffae Fructus Retinervus(LFR) has effects of anti-aging, disease-protection, long-life, etc.

• KSBB Journal
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• 제18권3호
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• pp.165-169
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• 2003

쇠비름 (Portulaca oleracea)의 항산화 효과를 탐색하기 위해 MeOH과 CH$_2$Cl$_2$를 이용해서 추출하여 crude extract를 조제하였다. 그리고 이 조추출물 (crude extract)에 대해 n-hexane, 15% aq. MeOH, EtOAc, n-BuOH, $H_2O$로 순차적으로 용매분획하였다. 쇠비름의 조추출물과 각 분획물들에 대해 DPPH radical 소거능과 마우스 간 균질물 (homogenate)을 이용한 과산화지질 억제능을 측정하였다. 그 결과 쇠비름 조추출물이 DPPH 라디칼에 대한 강한 소거작용과 in vitro 과산화지질 억제효과가 있음이 확인되었다. 조추출물의 DPPH radical 소거효과에 대한 $IC_{50}$/은 53.33 $\mu\textrm{g}$/$m\ell$로 천연 항산화제인 L-ascorbic acid (1.25 $\mu\textrm{g}$/$m\ell$)와 a-tocopherol (6.99 $\mu\textrm{g}$/$m\ell$)보다는 약하지만, 합성 항산화제로 널리 사용되고 있는 BHT(87.84 $\mu\textrm{g}$/$m\ell$)보다는 월등히 뛰어난 라디칼 소거능이 관찰되었다. 또한 각 분획물 중 EtOAc와 n-BuOH 분획물의 DPPH라디칼 소거효과가 가장 우수하였다. 마우스 간 균질물(mouse liver homogenate)을 이용한 과산화지질 저해효과에 대해서도 쇠비름 조추출물의 농도의존적인 (0.1 mg/$m\ell$~5 mg/$m\ell$) 상관성이 관찰되었으며, 특히 n-BuOH 분획물은 L-ascorbic acid의 효과와 거의 대등한 것을 나타났다. 이것으로 쇠비름 조추출물이 항산화효과 (in vitro)가 있음이 증명되었으며, 이 항산화활성은 극성이 비교적 큰 화합물들에 의한 것임을 추정할 수 있다. 현재 쇠비름 추출물로부터 항산화활성성분을 분리하기 위한 연구가 진행 중이다.

• 대한본초학회지
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• 제21권1호
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• pp.17-24
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• 2006

Objectives : This study was conducted to compare antioxidant activity of ratio-related Angelicae Radix and Astragali Radix compositions by DPPH radical scavenging activity. Methods : The species of Angelicae Radix was compared as Angelica gigas, A. sinensis and A. acutiloba. Then various compositions of Angelicae Radix with Astragali Radix were prepared with different ratio. Diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity was measured after 10, 20 and 30 minutes. Each extracts were tested by 1, 5, 10, 50, 100, 500 and 1,000 ${\mu}g/ml$ concentrations. Results : The results showed that Angelica gigas, A. sinensis and A. acutiloba scavenged DPPH radical with 66.8, 61.7 and 56.7% in 1,000 ${\mu}g/ml$ concentration, respectively. According to this result, Angelicae Radix and Astragali Hadix compositions with 100:0, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10 and 0:100 ratio were scavenged DPPH radical with 56.3, 49.9, 61.2, 55.7, 54.7, 55.0, 52.8 ,md 50.2% in 1,000 ${\mu}g/ml$ concentration, respectively. The extract of Angelicae Radix and Astragali Radix compositions with 100:0, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10 and 0:100 ratio scavenged DPPH radical with the $IC_{50}$ being 85,21, 1016.26, 656.51, 785.67 and 956.74 ${\mu}g/ml$ respectively. Conclusion : In conclusion, the extract of Angelicae Radix and Astragali Radix compositions with 1:1 ratio showed dose-dependent DPPH radical scavenging activity.

• 한국식품저장유통학회지
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• 제19권2호
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• pp.229-234
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• 2012

양파의 매운 맛과 냄새를 감소시켜 섭취가 용이하도록 제조된 흑양파가 항산화 활성이 있다고 보고되어있어서 흑양파로부터 항산화 활성물질을 구명하고자 하였다. 흑양파 MeOH 추출물을 용매분획하여 얻어진 분획물들 중 EtOAc층이 높은 DPPH와 $ABTS^+$ radical scavenging 활성이 있었다. 그래서 EtOAc층을 silica gel과 Sephadex LH-20 등의 column chromatography를 이용하여 2종의 항산화 활성물질을 분리하였다. 단리한 이들 화합물을 대상으로 ESI-MS 및 NMR 분석을 통하여 3,4-dihydroxybenzoic acid (1)와 quercetin (2)로 각각 동정하였다. 이 화합물들은 $ABTS^+$ 및 DPPH radical scavenging 활성이 있었으며, 화합물 2는 화합물 1에 비해 더 높은 radical scavenging 활성이 있었다.

• Journal of Acupuncture Research
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• 제21권5호
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• pp.69-78
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• 2004

Objective : This study was undertaken to determine the inhibitory effect of Whallak-tang against Nitric Oxide, the scavenging effect of Whallak-tang on DPPH radical. Methods : Both nitric concentration 1, 3, 6 and 24 hours after treatment with Vit. C and Whallak-tang at 0.1, 1, 10 mg/ml and scavenging effect 10, 20, 30 and 60 minute after treatment with Vit. C and Whallak-tang at 0.1, 1, 10 mg/ml were estimated. Results : The inhibitory effect of Whallak-tang against Nitric Oxide was dose-dependent. The scavenging effect of Whallak-tang on DPPH radical was dose-dependent. Conclusion : These results would provide important basic data on the possibility of the clinical treatment of Whallak-tang in musculoskeletal disease.

• 생약학회지
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• 제41권3호
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• pp.180-184
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• 2010

The antioxidant activity of Desmodium oldhami (Leguminosae) was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl). The methanolic extract of D. oldhami showed the strong antioxidant activity. Five compounds, kaempferol-3-O-rhamnopyranoside (afzeline) (1), quercetin-3-O-[$\alpha$-L-rhamnopyranosyl($1{\rightarrow}6$)-$\beta$-Dglucopyranoside] (rutin) (2), kaempferol-3-O-glucopyranoside (astragalin) (3), genistein-7-O-$\beta$-D-glucopyranoside (genistin) (4), kaempferol-3-O-rutinoside (5) were isolated from the active ethyl acetate soluble fraction of D. oldhami through repeated silica gel and Sephadex LH-20 column chromatography. Among them, compound 2 showed the most significant antioxidative effect on DPPH free radical scavenging test. Compounds 1-5 are reported for the first time from this plant.

• Food Science and Biotechnology
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• 제15권3호
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• pp.482-484
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• 2006

The antioxidant activity of Bacillus polyfermenticus SCD was studied by partially purified culture extracts using various methods: ammonium sulfate precipitation, adsorption to Diaion HP-20 columns using polar solvents, and extraction using non-polar solvents. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of these partially purified fractions was then investigated. The precipitate isolated using 75%-saturated ammonium sulfate was shown to contain about 77.2% DPPH radical scavenging activity. Using the Diaion HP-20 resin adsorption method, the fraction obtained using 60% ethanol and 60% methanol possessed 76.7 and 89.5% DPPH radical scavenging activity, respectively. Fractions obtained by extracting with the non-polar solvents 80 mg/mL chloroform, 80 mg/mL n-hexane, 80 mg/mL ethyl acetate, and 80 mg/mL butanol contained 68.4, 75.0, 70.7, and 87.5% DPPH radical scavenging activity, respectively. Further study is needed to characterize the antioxidant substance(s) released by B. polyfermenticus SCD cultures.

• Natural Product Sciences
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• 제10권5호
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• pp.244-247
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• 2004

The inhibitory effects of the leaves of Eucalyptus darylmpleana (Myrtaceae) on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The scavenging effect of the ethyl acetate fraction of Eucalyptus darylmpleana leaves on DPPH radical was stronger than the other fractions, and further purified by silica gel and Sephadex LH-20 column chromatography. 3,4-Dihydroxybenzoic acid, gallic acid, quercetin, quercetin $3-O-{\alpha}-_L-rhamnoside$, quercetin $3-O-{\beta}-_D-glucoside$ and quercetin 3-O-rutinoside were isolated and elucidated by spectroscopic data. Among these components, gallic acid and quercetin $3-O-{\alpha}-_L-rhamnoside$ exhibited potent scavenging activities on DPPH radical with $IC_{50}$ values of 6.02 and $5.54\;{\mu}M$, respectively.