• Title/Summary/Keyword: DPH

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Analysis of Red Coverage in Red- and White-koi Carp (Cyprinus carpio) and Red- and White-koi Carp (C. carpio)×Red Common Carp (C. carpio) Cross Progenies (홍백 비단잉어와 홍백 비단잉어×홍잉어 교배종의 적색소 분석)

  • Hwang, Ju-ae;Kim, Jung Eun;Lee, Jeong-Ho;Kim, Dae-Hee;Kim, Hyeong Su
    • Korean Journal of Ichthyology
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    • v.30 no.4
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    • pp.238-241
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    • 2018
  • The purpose of this study was to investigate color pattern and growth in cross progenies between kois and red common carp (Cyprinus carpio). Coverage of red color patches in skin was investigated in $koi{\times}koi$ (KK), $koi{\times}red$ common carp (KR) and red common $carp{\times}koi$ (RK) progenies in 170 days post-hatching (DPH) by analysis of digital photographs. KR cross group had higher length (P<0.05) and the mean weight than in the KK but there were no significant difference between KR and RK. All groups consisted of three color pattern white, white-red and red. The percentage of red-area coverage in skin was 64% in KK progenies, 56% in KR and 36% in RK. The red coverage (30~50%) was highly in KR (15%) than in KK (10%) and RK (12%). The application of red-area body coverage analysis may suggest potentially useful tool for ornamental fish selection.

The Effect of Methanol on the Structural Parameters of Neuronal Membrane Lipid Bilayers

  • Joo, Hyung-Jin;Ahn, Shin-Ho;Lee, Hang-Rae;Jung, Sung-Woo;Choi, Chang-Won;Kim, Min-Seok;Bae, Moon-Kyoung;Chung, In-Kyo;Bae, Soo-Kyoung;Jang, Hye-Ock;Yun, Il
    • The Korean Journal of Physiology and Pharmacology
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    • v.16 no.4
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    • pp.255-264
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    • 2012
  • The structures of the intact synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortexs, and the outer and the inner monolayer separately, were evaluated with 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) as fluorescent reporters and trinitrophenyl groups as quenching agents. The methanol increased bulk rotational and lateral mobilities of SPMVs lipid bilayers. The methanol increased the rotational and lateral mobilities of the outer monolayers more than of the inner monolayers. n-(9-Anthroyloxy)stearic acid (n-AS) were used to evaluate the effect of the methanol on the rotational mobility at the 16, 12, 9, 6, and 2 position of aliphatic chains present in phospholipids of the SPMVs outer monolayers. The methanol decreased the anisotropy of the 16-(9-anthroyloxy)palmitic acid (16-AP), 12-(9-anthroyloxy)stearic acid (12-AS), 9-(9-anthroyloxy)stearic acid (9-AS), and 6-(9-anthroyloxy)stearic acid (6-AS) in the SPMVs outer monolayer but it increased the anisotropy of 2-(9-anthroyloxy)stearic acid (2-AS) in the monolayers. The magnitude of the increased rotational mobility by the methanol was in the order at the position of 16, 12, 9, and 6 of aliphatic chains in phospholipids of the outer monolayers. Furthermore, the methanol increased annular lipid fluidity and also caused membrane proteins to cluster. The important finding is that was far greater increase by methanol in annular lipid fluidity than increase in lateral and rotational mobilities by the methanol. Methanol alters the stereo or dynamics of the proteins in the lipid bilayers by combining with lipids, especially with the annular lipids. In conclusion, the present data suggest that methanol, in additions to its direct interaction with proteins, concurrently interacts with membrane lipids, fluidizing the membrane, and thus inducing conformational changes of proteins known to be intimately associated with membranes lipids.