• Title/Summary/Keyword: DOT 4.2

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Rapid Dot-Blot Immunoassay for Detecting Multiple Salmonella enterica Serotypes

  • Jeongik Cho;Heymin Song;Hyun C. Yoon;Hyunjin Yoon
    • Journal of Microbiology and Biotechnology
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    • v.34 no.2
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    • pp.340-348
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    • 2024
  • Salmonella, a major contributor to foodborne infections, typically causes self-limiting gastroenteritis. However, it is frequently invasive and disseminates across the intestinal epithelium, leading to deadly bacteremia. Although the genus is subdivided into >2,600 serotypes based on their antigenic determinants, only few serotypes are responsible for most human infections. In this study, a rapid dot-blot immunoassay was developed to diagnose multiple Salmonella enterica serotypes with high incidence rates in humans. The feasibility of 10 commercial antibodies (four polyclonal and six monoclonal antibodies) was tested using the 18 serotypes associated with 67.5% Salmonella infection cases in the United States of America (U.S.A) in 2016. Ab 3 (polyclonal; eight of 18 serotypes), Ab 8 (monoclonal; 13 of 18 serotypes), and Ab 9 (monoclonal; 10 of 18 serotypes) antibodies exhibited high detection rates in western blotting and combinations of two antibodies (Ab 3+8, Ab 3+9, and Ab 8+9) were applied to dot-blot assays. The combination of Ab 3+8 identified 15 of the tested 18 serotypes in 3 h, i.e., S. Enteritidis, S. Typhimurium, S. Javiana, S. I 4,[5],12:i:-, S. Infantis, S. Montevideo, S. Braenderup, S. Thompson, S. Saintpaul, S. Heidelberg, S. Oranienburg, S. Bareilly, S. Berta, S. Agona, and S. Anatum, which were responsible for 53.7% Salmonella infections in the U.S. in 2016. This cost-effective and rapid method can be utilized as an on-site colorimetric method for Salmonella detection.

Optical and Electrical Properties of InAs Sub-Monolayer Quantum Dot Solar Cell

  • Han, Im-Sik;Park, Dong-U;No, Sam-Gyu;Kim, Jong-Su;Kim, Jin-Su;Kim, Jun-O
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.196.2-196.2
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    • 2013
  • 본 연구에서는 분자선 에피택시 (MBE)법으로 성장된 InAs submonolayer quantum dot (SML-QD)을 태양전지에 응용하여 광학 및 전기적 특성을 평가하였다. 본 연구에서 사용된 양자점 태양전지(quantum dot solar cell, QDSC)의 구조는 n+-GaAs 기판 위에 n+-GaAs buffer와 n-GaAs base layer를 차례로 성장 한 후, 활성영역에 InAs/InGaAs SML-QD와 n-GaAs spacer layer를 8주기 형성하였다. 그 위에 p+-GaAs emitter, p+-AlGaAs window layer를 성장하고 ohmic contact을 위하여 p+-GaAs 를 성장하였다. SML-QD 구조의 두께는 0.3 ML 이며, 이때 SML-QD의 적층수를 4 stacks 으로 고정하였다. SML-QD 와의 비교를 위하여 2.0 ML크기의 InAs자발 형성 양자점 태양전지(SK-QDSC)과 GaAs 단일 접합 태양전지 (reference-SC)를 동일한 성장조건에서 제작하였다. PL 측정 결과, 300 K에서 SML-QD의 발광 피크는 SK-QD 보다 고에너지에서 나타나는데(1.349 eV), 이것은 SML-QD가 SK-QD보다 작은 크기를 가지기 때문으로 사료된다. SML-QD는 single peak를 보이는 반면, SK-QD는 dual peaks (1.112 / 1.056 eV)을 확인하였다. SML-QD의 반치폭(full width at half maximum, FWHM)이 SK-QD에 비하여 작은 것으로 보아 SML-QD가 SK-QD보다 양자점 크기 분포의 균일도가 높은 것으로 해석된다. Illumination I-V 측정 결과, SML-QDSC의 개방 전압(VOC) 과 단락전류밀도(JSC)는 SK-QDSC의 값과 비교해 보면, 각각 47 mV와 0.88 mA/cm2만큼 증가하였다. 이는 SK-QD보다 상대적으로 작은 크기를 가진 SML-QD로 인해 VOC가 증가되었으며, SML-QD가 SK-QD 보다 태양광을 흡수할 수 있는 영역이 비교적 적지만, QD내에 존재하는 energy level에서 탈출 할 수 있는 확률이 더 높음으로써 JSC가 증가한 것으로 분석 된다.

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CdSe Quantum Dots Sensitized TiO2 Electrodes for Photovoltaic Cells

  • Yum, Jun-Ho;Choi, Sang-Hyun;Kim, Seok-Soon;Kim, Dong-Yu;Sung, Yung-Eun
    • Journal of the Korean Electrochemical Society
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    • v.10 no.4
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    • pp.257-261
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    • 2007
  • The electronic properties of quantum dots can be tuned by changing the size of particles without any change in their chemical composition. CdSe quantum dots, the sizes of which were controlled by changing the concentrations of Cd and Se precursors, were adsorbed on $TiO_2$ photoelectrodes and used as sensitizers for photovoltaic cells. For applications of CdSe quantum dot as sensitizers, $CdSe/TiO_2$ films on conducting glass were employed in a sandwich-type cell that incorporated a platinum-coated conductive glass and an electrolyte consisting of an $I^-/I_3^-$ redox. The fill factor (FF) and efficiency for energy conversion ($\c{c}$) of the photovoltaic cell was 62 % and 0.32 %, respectively.

High Yield Saponin Production by Mass Cultures of Ginseng Transformed Tissue I. Induction, Culture of Transformed Tissue and Selection of High-Saponin-Producing Clones in Ginseng (인삼 형질전환 조직의 다량배양에 의한 Saponin 고 생산 I. 인삼에서 형질전환 조직의 유도, 배양과 Saponin 고 생산능주 선발)

  • 이정석;고경민
    • KSBB Journal
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    • v.9 no.2
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    • pp.157-164
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    • 1994
  • Hairy root clones of Panax ginseng were established by selection of some hairy roots formed on the leaf, stem and root segments transformed with Agrobacterium rhizogenes strain $A_4$. The transformed roots grew well in MS medium under the dark condition. To confirm the transformation with Ri-T-DNA, dot blot hybridization and opine analysis were Performed. Among four hairy roots induced from different part of ginseng, the HB3 hairy roots were examined for selection of high-saponin-producing clones. Four clones isolated from HB3 hairy root cultures displayed various phenotypes characterized by growth and total saponin content. Maximum growth was obtained for cultures of HB3-10 clone and the content of total saponin was 0.55 wt%. However, higher amount of total saponin was obtained with HB3-2 clone cultures(0.74 wt%) in spite of lower growth. Dot blot hybridization confirmed the introduction of Ri-T-DNA in the plant genome. In the opine test, agropine and mannopine were detected from all hairy root clones.

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Optimization of Fabrication Conditions for Cu2S Counter Electrodes of Quantum Dot-Sensitized Solar Cells (양자점 감응 태양전지의 Cu2S 상대 전극 제작조건 최적화)

  • JUNG, SUNG-MOK;HA, SEUNG-BEOM;SEO, JOO-WON;KIM, JAE-YUP
    • Transactions of the Korean hydrogen and new energy society
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    • v.32 no.6
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    • pp.663-668
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    • 2021
  • For the development of highly efficient quantum dot-sensitized solar cells (QDSCs), it is important to enhance the electrocatalytic activity of the counter electrodes (CEs). Herein, a fabrication process of Cu2S CEs are optimized for the development highly efficient QDSCs. The surface of brass film is treated with HCl solution to prepare the Cu2S CEs, and the concentraion as well as the temperature of HCl solution are controlled. It is found that the uniformity for the thickness of prepared Cu2S CEs is enhanced when the diluted HCl solution is used, compared to the HCl solution of standard concentration. In addition, the electrocatalytic activity of the Cu2S CEs is also increased with the modificed process, which is confirmed by impedance data and Tafel polarization curves. As a result, the photoconversion efficiency of QDSCs is improved from 4.49% up to 5.73%, when the concentraion and temperature of the HCl treatment are efficiently optimized.

THE ABSORPTION PROPERTIES OF Cu-Zn FERRITE/RUBBER COMPOSITE MICROWAVE ABSORBER WITH PZT ADDITIVE

  • Shin, Kwang-Ho;Geon, Sa-Gong
    • Journal of the Korean Magnetics Society
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    • v.5 no.5
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    • pp.556-560
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    • 1995
  • The absorption properties of Cu-Zn ferrite/rubber composite microwave absorbers with PZT(Lead Zirconate Titanate) additive were evaluated. The composite specimens have prepared by molding and curing the mixture of matrix rubber and Cu-Zn ferrite powders which are synthesized by the coprecipitation method using Fe Cl/sub 3/ .center dot. 6H/sub 2/O, CuCl/sub 2/ .center dot. 2H/sub 2/O and Zn Cl/sub 2/ as a starting raw materials. PZT is used as another filler particles to adjust the material constants of Cu-Zn ferrite/rubber composite specimens. We have found that the material constants of specimens could be controlled by various PZT mixing ratio. On the Cu-Zn ferrite/rubber composite specimens with PZT 10[wt%] additive, the reflection losses were larger than 30[dB] in the frequency range from 2.72 to 4.4[GHz]' by adjusing the thickness.

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Implementing Tactile Display via Electromagnetic Actuator (전자석 액추에이터를 통한 촉각 디스플레이 구현)

  • Kim, Ju Yoon;Sung, Ki Kwang;Kim, Ji Ho;Park, Hyeon Cheol;Choi, Ah Rum
    • Journal of Appropriate Technology
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    • v.6 no.2
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    • pp.146-150
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    • 2020
  • Assistive technologies for people with disabilities are often marginalized in the Fourth Industrial Revolution. In this paper, we intend to present the possibility of acquiring tactile information through the tactile display adapting an electromagnetic actuator. The multi-layered display designed for tactile communication has innovatively narrowed the size and spacing of cells in order to express pictures, maps, and graphics. This display has enabled the visually impaired to touch two-dimensional information combining with other assistive technologies so that. It would also provide a technical source of access to STEM education (Science, Technology, Engineering, and Mathematics) so that students will perform better and be better prepared for advanced education. Moreover, it can be applied to public infrastructure and transportation in smart-city projects on the globe. Hence, this paper introduces the principle of Electromagnetic-actuator and how it can be utilized in many diverse areas.

The Effectiveness of the Random-dot E by the Difference of the Illumination and Test Distance (조도와 검사 거리의 차이에 의한 Random-dot E의 영향)

  • Kim, Douk-Hoon
    • Journal of Korean Ophthalmic Optics Society
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    • v.5 no.2
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    • pp.1-4
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    • 2000
  • The test of stereoacuity provides relatively accurate assessment of binocular functior. in the clinical fields. The purpose of this study was to investigate performance on the Random-dot E(RDE) stereotest under the binocular conditions by the difference of the illumination and test distance. The more light illumination increase. The more pass of RDE stereotest increase. On the other hand. On the near distance of test target. All subjects have a pass toe RDE stereatest. But On the far distance of test target. Some subjects have a pass the RDE stereatest. According to the test distance. The more far distance of test target. the less subjects have a pass the RDE stereotest. As a results, The RDE stereotest have effected the test distance and illumination.

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Predicting of Fire Characteristics of Flame Retardant Treated Douglas fir Using an Integral Model (적분모델을 이용한 난연처리된 Douglas fir의 화재특성 예측)

  • Park, Hyung-Ju;Kim, Hong;Ha, Dong-Myeong
    • Journal of the Korean Society of Safety
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    • v.20 no.3 s.71
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    • pp.98-104
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    • 2005
  • This study experimentally and theoretically examines the fire characteristics of 100- by 100- by 50-mm samples of flame retardant treated Douglas fir. Samples were exposed to a range of incident heat fluxes 10 to $50kW/m^2$. The time to ignition measurements obtained from the cone heater were used to derive characteristic properties of the materials. A one-dimensional integral model has been used to predict the, time to ignition, critical heat flux and ignition temperature of samples. Ignition data and best-fit curves confirm ${{\dot{q}}_i}^{'}{\rightarrow}{{\dot{q}}_{cr}^{'}\;then\;t_{ig}{\rightarrow}{\infty}$ and when ${{\dot{q}}_i}^'{\gg}{{\dot{q}}_{cr}^'\;then\;t_{ig}{\rightarrow}0$. And Ignition of flame retardant treated samples occurred not at incident heat flux of bellow $10kW/m^2.$. By a one-dimensional integral model, the critical heat flux of each samples was predicted $10.21kW/m^2,\;11.82kW/m^2,\;and\;14.16kW/m^2$ for the D-N, D-F2, and D-F4, respectively. In ignition temperature of each samples, flame retardant treated samples were measured high about $50^{\circ}C$ than non-treated samples. Water-soluble flame retardant used in this study finds out more effect in delay of time to ignition when incident heat flux is low than high.

Ferroelectric ultra high-density data storage based on scanning nonlinear dielectric microscopy

  • Cho, Ya-Suo;Odagawa, Nozomi;Tanaka, Kenkou;Hiranaga, Yoshiomi
    • Transactions of the Society of Information Storage Systems
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    • v.3 no.2
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    • pp.94-112
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    • 2007
  • Nano-sized inverted domain dots in ferroelectric materials have potential application in ultrahigh-density rewritable data storage systems. Herein, a data storage system is presented based on scanning non-linear dielectric microscopy and a thin film of ferroelectric single-crystal lithium tantalite. Through domain engineering, we succeeded to form an smallest artificial nano-domain single dot of 5.1 nm in diameter and artificial nano-domain dot-array with a memory density of 10.1 Tbit/$inch^2$ and a bit spacing of 8.0 nm, representing the highest memory density for rewritable data storage reported to date. Sub-nanosecond (500psec) domain switching speed also has been achieved. Next, long term retention characteristic of data with inverted domain dots is investigated by conducting heat treatment test. Obtained life time of inverted dot with the radius of 50nm was 16.9 years at $80^{\circ}C$. Finally, actual information storage with low bit error and high memory density was performed. A bit error ratio of less than $1\times10^{-4}$ was achieved at an areal density of 258 Gbit/inch2. Moreover, actual information storage is demonstrated at a density of 1 Tbit/$inch^2$.

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