• Title/Summary/Keyword: DNT

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Microbiological Elimination of 2,4-Dinitrotoluene and 276-Dinitro-toluene by an TNT-degrading Bacterium in Stirred Tank Reactors (교반탱크 반응조에서 TNT 분해세균에 의한 2,4-Dinitrotoluene/2,6-Dinitrotoluene의 미생물학적 제거)

  • 장효원;김승일;오계헌
    • Korean Journal of Microbiology
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    • v.37 no.1
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    • pp.66-71
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    • 2001
  • An aerobic microbiological process was tested in 1.5 L stirred tank reactors for the treatment of dinitrotoluenes (DNTs)[e.g., 2.4-dinitrotoluene (2,4-DNT), 2,6-dinitrotoluene (2,6-DNT)] in the test culture of Stenotrophomonas maltophilia, which had previously characterized. Both 2,4-DNT and 2,6-DNT were completely degraded within 10 days and 14 days of incubation, respectively. Addition of the secondary carbon was essential to degrade DNTs, and little degradation was achieved in the absence of the secondary carbons. The effect of additional nitrogens on the degradation of DNTs was evaluated. Complete degradation of DNTs was observed in the absence of any additional nitrogens, whereas DNTs were partially degraded in the growth media with additional nitrogens. Both HPLC and GC-MS were used to detect and verify the residual DNTs and their intermediates. As the results, the HPLC and GC-MS chromatograms demonstrated that the both parent compounds, 2, 4-DNT and 2,6-DNT, and respective intermediates, 2-amino-4-nitrotoluene and 2-amino-6-nitrotoluene, could be successfully identified under the analytical conditions.

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Analysis and Proposal of "Do Not Track" Regulations for Online Behavioral Advertising (온라인 행동기반 맞춤형 광고를 위한 온라인 추적 금지 제도 분석 및 제안)

  • Choi, Jinju;Lee, Chunghun;Kim, Beomsoo
    • The Journal of Society for e-Business Studies
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    • v.17 no.4
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    • pp.155-174
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    • 2012
  • As Online Behavioral Advertising is dramatically growing with usefulness of information and user convenience in recent years, there are privacy issues caused by collecting user's behavioral information without their consent. To tackle the problem, the need of Do Not Track regulations is getting much higher. In Korea, however, it has never existed. So, this study is examining the case of the major countries have been enforcing the law and regulations of DNT. After that, it is classified with four domain (law/regulation, corporation, individual, society) to include all stakeholders of OBA. Furthermore, this study may have academic significance by suggesting DNT framework through analysis of them. Providing DNT mechanism consisted of three type (behavioral information, control, DNT system), it can be useful guidelines for companies to support decision making as introduced DNT. As analyzed between DNT and stakeholders based on the study of OBA market, it will be useful basic material of OBA study later.

Identification of Dinitrotoluene Selective Peptides by Phage Display Cloning

  • Jang, Hyeon-Jun;Na, Jung-Hyun;Jin, Bong-Suk;Lee, Won-Kyu;Lee, Woong-Hee;Jung, Hyun-Jin;Kim, Seok-Chan;Lim, Si-Hyung;Yu, Yeon-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.31 no.12
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    • pp.3703-3706
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    • 2010
  • Biomolecules specific to explosives can be exploited as chemical sensors. Peptides specific to immobilized dinitrotoluene (DNT) were identified using a phage display library. A derivative of DNT that contained an extended amine group, 4-(2,4-dinitrophenyl)butan-1-amine, was synthesized and immobilized using a self-assembled monolayer surface on gold. Filamentous M13 phages displaying random sequences of 12-mer peptides specific to the immobilized DNT-derivate were isolated from the M13 phage library by biopanning. A common peptide sequence was identified from the isolated phages and the synthesized peptides showed selective binding to DNT. When the peptide was immobilized on a quartz crystal microbalance (QCM) chip, it showed a binding signal to DNT, while toluene barely showed significant binding to the QCM chip. These results demonstrate that peptides screened by biopanning against immobilized DNT can be useful for quick and accurate detection of DNT.

Development of a Single Chain Antibody Using a Phage Display Cloning Method for the Detection of 2,4-Dinitrotoluene

  • Na, Jung-Hyun;Joo, Man-Seok;Lee, Won-Kyu;Shim, Hyunbo;Lim, Si-Hyung;Jung, Sang Taek;Yu, Yeon Gyu
    • Bulletin of the Korean Chemical Society
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    • v.34 no.2
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    • pp.460-464
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    • 2013
  • Single-chain variable fragments of antibodies (scFv) specific to 2,4-dinitrotoluene (DNT) were isolated from a phage library displaying synthetic human scFv fragments with 6 diversified complementary determining regions (CDRs). A DNT derivative that contained an extended amine group was synthesized and conjugated to the NHS-group that was linked to magnetic beads. Phages specific to the immobilized DNT derivatives were isolated from the library after 4 rounds of sequential binding and elution processes. The displayed scFv fragments from the isolated phages showed consensus CDR sequences. One DNT-specific scFv was expressed in E. coli and purified using Ni-affinity chromatography. The purified DNT-specific scFv binds specifically to the immobilized DNT-derivative with $K_D$ value of $6.0{\times}10^{-7}$ M. The scFv and DNT interaction was not disrupted by the addition of 4-nitrotoluene or benzoic acid. These data demonstrate that the screened scFv from the phage displayed library could be used for selective and sensitive detection of explosives such as TNT.

Performance Evaluation of Explosive Specific Bio-receptor Using QCM Sensing Platform for Resonance Frequency Shift Detection (공진점변화검출용 QCM 센싱플랫폼을 이용한 폭발물 특이적 바이오수용체 성능평가)

  • Lim, Si-Hyung;Jeong, Hyun-Jin
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.21 no.3
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    • pp.280-284
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    • 2011
  • The mass change during the molecular interaction between explosive specific bio-receptors and target molecules has been measured using quartz crystal microbalance(QCM), which has a mass change detection limit up to ~ng/$cm^2$. The environmental effect on the molecular interaction has been evaluated. In the liquid phase molecular interaction experiments, the high selectivity of the bio-receptor to DNT compared with toluene has been shown and the sensitivity for various concentrations of DNT has been demonstrated.

An IM Driving Power Electronic System Simulation using DNT and Simulink (DNT와 Simulink를 이용한 유도전동기 구동용 전력전자 시스템 시뮬레이션)

  • Moon-Ho Kang
    • Journal of Institute of Control, Robotics and Systems
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    • v.5 no.1
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    • pp.25-32
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    • 1999
  • 교류전동기 구동을 위한 산업용 전력전자 시스템은 전력회생용 양방향 컨버터, VVVF 인버터, 교류전동기 벡터제어기, 및 다양한 전기ㆍ기계적 연계 장치들을 포함하는 전기기계 복합시스템 특성을 지니기 때문에 전체 시스템에 대한 컴퓨터 시뮬레이션이 용이하지 않다. 일반적으로 이러한 복잡성을 해결하기 위한 방안으로서 시스템을 근사화시키고 이에 대한 수학적인 등가모델을 유도한 후 이를 이용하여 시뮬레이션을 행하고 있지만, 이 경우 실계통의 다이나믹특성을 정확히 표현할 수 없기 때문에 시뮬레이션 신뢰성이 저하될 수 있다 본 논문에서는 가변구조 제어기법에 의해 역률1 제어기능을 갖는 PWM 컨버터, VVVF 인버터 및 유도기 간접벡터제어기 둥을 포함하는 유도전동기 구동전력전자 시스템을 다이나믹 노드 기법(Dynamic Node Technique DNT)과 시뮬링크(Simulink)를 이용하여 실제회로 구성상태와 유사한 형태로 시뮬링크 윈도우상에서 설계하고, 이를 통해 시뮬레이션을 행할 수 있는 기법을 제시한다. 시뮬레이션 결과를 통하여 전력전자 시스템의 특성과 제시된 다이나믹 노드 기법과 시뮬링크를 이용한 시뮬레이션 기법의 유용성을 보였다.

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Facile Fabrication of Aligned Doubly Open-ended TiO2 Nanotubes, via a Novel Selective Etching Process, and Thier Application in Dye Sensitized Solar Cells

  • Choe, Jong-Min;Park, Tae-Ho
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.483.2-483.2
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    • 2014
  • In this study, we describe a simple selective etching method that produces noncurling, freestanding, large-area, aligned $TiO_2$ nanotube (NT) with doubly ends opened. The novel selective etching process only removed the thin 2nd bottom layer from the physically and chemically stable thick amorphous 1st top layer under thermal treatment at $250^{\circ}C$, yielding ordered doubly open-ended NT (DNT) that could be easily transferred to an FTO substrate for the fabrication of front-illuminated dye sensitized solar cells (DSCs). The DNT-DSCs yielded a higher PCE (8.6%) than was observed from $TiO_2$ nanoparticle (TNP)-based DSCs (7.3%), for comparable film thicknesses of $16{\mu}m$, despite of 20% decreased amount of dye. Intensity-modulated photocurrent and photovoltage spectroscopy (IMPS and IMVS, respectively) revealed that the DNT-DSCs exhibited electron lifetimes that were 10 times longer than those of TNP-DSCs, which contributed to high device performances.

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멀티스케일 해석을 통한 홀리데이 연결 물성 분석

  • Lee, Jae-Gyeong;Kim, Tae-Hwan
    • Proceeding of EDISON Challenge
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    • 2017.03a
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    • pp.340-345
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    • 2017
  • DNA 나노구조물을 설계 및 개발, 해석하기 위해서는 기본적인 홀리데이 연결(Holliday junction) 구조에 대한 물성을 아는 것이 필수적이다. 여러 실험 및 시뮬레이션을 통해서 홀리데이 연결 구조 물성을 측정하려는 시도가 많았지만, 아직까지도 홀리데이 구조에 대한 정확한 물성은 얻어지지 않았다. 이번 연구에서는 6HB-DNT 모델을 분자동역학 기법을 이용하여 DNT 모델의 물성을 분석하고, 이를 기반으로 유한요소 모델링을 통해 홀리데이 연결의 물성을 해석한다.

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Estimation of Harmonics on Power System of AC Electric Railway (교류 전기철도 전력계통의 고조파 예측량 계산)

  • 송진호;황유모
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.52 no.2
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    • pp.68-79
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    • 2003
  • We estimated harmonics on power system of AC railway based on quantitatively measured harmonics and investigated the need of facilities for harmonics reduction. In order to analysis harmonics which inflow into power system due to increase in collector voltages and harmonic currents generated from the train when the railway is in operation, the railway system Is sectioned into power supply, railway line, AT, sectioning Post and subsectioning post. For analysis of extension of currents resulting from the railway loads, PWM converter, VVVF inverter and the feeder system are modeled based on the dynamic node technique(DNT). In order to test the usefulness of the DNT for analysis of harmonic effects, the measured harmonic currents and harmonic magnification ratios at the S/K substation are compared with simulation results using DNT modelling, which include the results for two cases with and without filters for suppression of harmonic currents. When 8 cars(4M4T) are in operation, the total sum of harmonic currents resulting from the train at M and T phases, which inflow into the substation along with the railway line, is calculated. Using the harmonics analysis program for railway feeder system with these data, the total harmonic distortion factor(710) at the outgoing point of KEPCO substation is computed. The calculation shows that when the maximum THD at the receiving point of H/K substation was 0.0443% which is much lower than 1.5% which is the allowable value of KEPCO at 154kV as well as IEEE-519 above 132kV This result indicates that any measure for harmonics reduction in Incheon International Airport Railway is not needed.

영가 철로 구성된 Flow-Through Column내에서 미생물 처리에 이한 폭발성 물질의 제거 향상

  • 오병택;윤제용
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.73-76
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    • 2004
  • Rusted iron could retain activity to redox-sensitive pollutants in batch reactor. Flow-through columns packed with permeable reactive iron filings (Fe$^{0}$ ) between soil and sand layers were used to evaluate the applicability of bio-enhanced iron barriers to treat explosives-contaminated groundwater. One column was bioaugmented with municipal anaerobic sludge to evaluate the enhancement of biodegradation. Military contaminants (RDX, HMX, TNT, 2,4DNT, 2,6DNT), which coexist in soils at military sites, were completely removed in the bioaugmented Fe$^{0}$ layer after 8 months of operation. Overall, this research suggests that Fe$^{0}$ barriers can effectively clean up groundwater contaminated with military explosives, and that treatment efficiency can be enhanced by bioaugmentation.

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