• Title/Summary/Keyword: DNS

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Isolation and characterization of microorganisms biological damage of Dongchundang (동춘당 생물학적 가해 미생물의 분리 및 특성)

  • Lee, Jeung-Min;Kim, Young-Hee;Hong, Jin-Young;Jo, Chang-Wook;Kim, Soo Ji;Seo, Min Seok
    • 보존과학연구
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    • s.35
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    • pp.111-119
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    • 2014
  • Microorganisms were isolated from Dongchundang(wooden cultural heritage) with PDA medium culture. Nineteen species shows the cellulolytic activity. Methylobacterium sp. was the most active in cellulose degradation. The growth curve and pH were measured during incubation of the microorganism for 72 hours. The pH was increased with the increasing of microbial growth. The degree of cellulose degradation was determined with the amount of reducing sugar by use of dinitrosalicylic acid (DNS) method. The amount of reducing sugar was decreased after 45 hours. As a results, It should suggested that wood component were deteriorated by Methylobacterium sp..

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Numerical Study on the Reacting Flow Field abound Rectangular Cross Section Bluff Body (사각 둔각물체 주위의 반응유동장에 대한 수치적 연구)

  • Lee, Jung-Ran;Lee, Eui-Ju
    • Fire Science and Engineering
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    • v.27 no.6
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    • pp.64-69
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    • 2013
  • The Numerical simulation was performed on the flow field around the two-dimensional rectangular bluff body in order to simulate an engine nacelle fire and to complement the previous experimental results of the bluff body stabilized flames. Fire Dynamic Simulator (FDS) based on the Direct Numerical Simulation (DNS) was employed to clarify the characteristics of reacting flow around bluff body. The overall reaction was considered and the constant for reaction was determined from flame extinction limits of experimental results. The air used atmosphere and the fuel used methane. For both fuel ejection configurations against an oxidizer stream, the flame stability and flame mode were affected mainly by vortex structure near bluff body. In the coflow configuration, air velocity at the flame extinction limit are increased with fuel velocity, which is comparable to the experiment results. Comparing with the isothermal flow field, the reacting flow produces a weak and small recirculation zone, which is result in the reductions of density and momentum due to temperature increase by reaction in the wake zone.

Protoplast Fusion of Fusarium oxysporum and activity of polygalacturonase (Fusarium of oxysporum의 원형질체 융합과 polygalacturonase의 활성)

  • Ha, Kyung-Ran;Min, Byung-Re
    • The Korean Journal of Mycology
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    • v.21 no.3
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    • pp.195-199
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    • 1993
  • This experiments was designed for development of Fusarium oxysporum strains with enhanced activity of polygalacturonase by means of mutants and protoplasts fusion. Six auxotrophic mutants of F. oxysporum were induced by treatment of MNNG. Protoplasts from mutants were formed from early exoponential mycelium after treatment with driselase(10 mg/ml) using 0.6 M KCl as osmotic stabilizer. Fusion experiments between protoplasts of several auxotrophic mutants were done using polyethyleneglycol 8,000 and $CaCl_2$. The frequency of fusion was $5.0{\times}10^{-3}$ as determined from several experiments. Activity of polygalacturonase was determined by the methods of modified DNS. Consequently, the polygalacturonase activities of mutants and fusant derived F. oxysporum were 1.4 to 3.5 times greater than that of the parental strain, and mutant Fx-2 seemed to be the best strain. Thus, the method we used seemed to be favorable for the improvement of strains.

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Classification of tree species using high-resolution QuickBird-2 satellite images in the valley of Ui-dong in Bukhansan National Park

  • Choi, Hye-Mi;Yang, Keum-Chul
    • Journal of Ecology and Environment
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    • v.35 no.2
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    • pp.91-98
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    • 2012
  • This study was performed in order to suggest the possibility of tree species classification using high-resolution QuickBird-2 images spectral characteristics comparison(digital numbers [DNs]) of tree species, tree species classification, and accuracy verification. In October 2010, the tree species of three conifers and eight broad-leaved trees were examined in the areas studied. The spectral characteristics of each species were observed, and the study area was classified by image classification. The results were as follows: Panchromatic and multi-spectral band 4 was found to be useful for tree species classification. DNs values of conifers were lower than broad-leaved trees. Vegetation indices such as normalized difference vegetation index (NDVI), soil brightness index (SBI), green vegetation index (GVI) and Biband showed similar patterns to band 4 and panchromatic (PAN); Tukey's multiple comparison test was significant among tree species. However, tree species within the same genus, such as $Pinus$ $densiflora-P.$ $rigida$ and $Quercus$ $mongolica-Q.$ $serrata$, showed similar DNs patterns and, therefore, supervised classification results were difficult to distinguish within the same genus; Random selection of validation pixels showed an overall classification accuracy of 74.1% and Kappa coefficient was 70.6%. The classification accuracy of $Pterocarya$ $stenoptera$, 89.5%, was found to be the highest. The classification accuracy of broad-leaved trees was lower than expected, ranging from 47.9% to 88.9%. $P.$ $densiflora-P.$ $rigida$ and $Q.$ $mongolica-Q.$ $serrata$ were classified as the same species because they did not show significant differences in terms of spectral patterns.

The Effect of Dynamic Neuromuscular Stabilization Exercise on Pulmonary Function and Electroencephalogram in Elderly (동적 신경근 안정화 운동이 노인들의 폐기능과 뇌파에 미치는 영향)

  • Ho-Kwan Ko;Seung-Chul Chon
    • Journal of The Korean Society of Integrative Medicine
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    • v.12 no.3
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    • pp.25-36
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    • 2024
  • Purpose : After coronavirus disease 2019 pandemic, many people around the world became aware of respiratory diseases and developed increased interest in health and exercise. As the elderly population grows larger, the prevalence of brain diseases and respiratory conditions increases. This study aimed to investigate the impact of dynamic neuromuscular stabilization (DNS) exercises on pulmonary function and electroencephalogram (EEG) findings in the elderly population. Methods : In this study, we measured the pulmonary function and performed EEG of 30 elderly individuals. The participants were randomly divided into an experimental group (n=15) and a control group (n=15). The experimental group completed a DNS exercise training protocol, while the control group performed simple exercise training. After the exercise, the pulmonary function and EEG testing were repeated, and the amounts of change within and between groups were determined. Results : In terms of pulmonary function, the experimental group's forced vital capacity (FVC) and forced expiratory volume at 1 second (FEV1) increased significantly after the exercise (p<.05), while the FVC differed significantly between groups (p<.05). In terms of EEG findings, the relative alpha waves significantly decreased in the F3 region (p<.05), while the relative beta waves significantly increased in the F4 region (p<.05). The relative gamma waves increased significantly in the Fp2 and P3 regions (p<.05), while the values in the F3 region differed significantly between groups (p<.05). Conclusion : DNS exercises performed by this elderly population demonstrated significant clinical value by providing fundamental data about both pulmonary function and EEG activity.

Dough Characteristics of Korean Wheat Flour (국산 밀가루의 반죽특성)

  • 정헌상;박남규;송정춘;김기종;정만재
    • Food Science and Preservation
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    • v.6 no.2
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    • pp.161-166
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    • 1999
  • In order to increase the Korean wheat-cultivators' incomes as well as to promote their consumptions, the native wheat of 11 varities (Chokwangmil, Geurumil, Eunpamil, Tapdongmil, Woorimil, Olgeurumil, Alchanmil , Gobunmil, Geungangmil Seodunmil and Suwon 265) were investigated on dough properties to compared with those of two imported wheat varieties, DNS (Dark Northern Spring Wheat) and ASW (Austrlian Standard White). In dough stickiness, Chokwangmil was highest as 81.78, and the mean value of native wheat was 53.98 g. ASW and DNS showed the stickiness values of 58.7 g and 52.9 g, respectively. Tapdongmil was highest in the tensile tone of dough as 87.6 g. In the texture test, the highest elasticity, gumminess, firmness and chewiness were observed in Woorimil as values of 0.980, 1,022.8, 1,562.7 and 1,001.9, respectively, while the highest cohesiveness and adhesiveness were obtained from Suwon 265 and Geurumil, respectively, as 0.710 and -609.4. In amylogram properties, the initial gelatinization temperature was ranged from 65$^{\circ}C$ to 69$^{\circ}C$, and maximum, minimum and final viscosities were highest in Woorimil as 1,140, 1,100 and 1,730 BU, respectively. In farinogram, water absorption rate was highest in Suwon 265 as 65.1%, which required the longest dough arrival time of 3.5 min. Dough developing time, weakening and stability were longest in Gobunmil as 9.7. 32.1 and 29.9 min., respectively. The resistance of dough was highest in Olgeurumil as 110 BU.

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Comparison of Physicochemical, Microbial and Antioxidant Properties in Domestic and Imported Wheat Kernels for Bread Making (제빵용 우리밀과 수입밀 원맥의 이화학, 미생물, 항산화 특성 비교)

  • Kwak, Han Sub;Kim, Mi Jeong;Heo, JeongAe;Kim, Min Jung;Shim, Jaewon;Kim, Oui-Woung;Kim, Hoon;Kim, Sang Sook
    • The Korean Journal of Food And Nutrition
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    • v.31 no.1
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    • pp.17-23
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    • 2018
  • The objective of this study was to compare physicochemical, microbial, and antioxidant properties of domestic and imported wheat kernels for bread making. Two domestic (JK1, 2) and three imported (ND, DNS, and CWRS) kernels were compared. Domestic kernels had higher moisture contents, and lower ash and protein contents (p<0.05). In grain characteristics, JK1 had 13.62% of damaged kernels, which was the highest among the samples (p<0.05). JK2 was similar to imported kernels in the ratio of sound kernels, foreign materials, and damaged kernels. Kernel size of JK1, 2 was larger than the imported kernels; therefore, kernels area and perimeter were higher by the image analyzer. Domestic kernels hid lower total aerobic counts the imported kernels (p<0.05). Domestic kernels and DNS had no yeast, while NS and CWRS had yeast in kernels. DNS (3.08 mg gallic acid equivalent (GAE)/g) had the highest total polyphenol content (TPC), followed by JK1 (2.81 mg GAE/g). JK2 had the lowest amount of TPC as 2.26 mg GAE/g. Total flavonoid content (TFC) was the highest in DNS as 0.44 mg catechin equivalent (CE)/g and JK2 was the lowest as 0.12 mg CE/g. Domestic wheat kernels had lower protein content and lightness than the imported wheat kernels so that flour from domestic wheat kernels may have lower quality for baking.

A Comparative Study of the Assay Methods Used to Quantify Fermentable Sugar in Makgeolli Sul-dut (막걸리 술덧의 발효성 당 분석방법 비교 연구)

  • Kim, Byong-Soo;Kim, Gye-Won;Shim, Jae-Yong
    • Korean Journal of Food Science and Technology
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    • v.48 no.1
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    • pp.48-53
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    • 2016
  • The objective of this study was to evaluate the accuracy and efficiency of different methods used for the fermentable sugar assay in the production of Makgeolli sul-dut. In the initial stage of fermentation, Ipguk treatment produced a higher alcohol content compared to the Nuruk treatment. However, the alcohol content was not significantly different between the two starters at the final stage of fermentation. Acidity in the Ipguk treatment was higher than that of Nuruk throughout the fermentation period. After analyzing the fermentable sugars using dinitrosalicylic acid (DNS), Fehling's method, refractometer, glucose kit, and high performance liquid chromatography (HPLC), it was confirmed that the HPLC method was the most accurate for fermentable sugar quantification. In both types of starters, DNS and Fehling's methods showed results comparable to HPLC in terms of fermentable sugar content, while the glucose kit and refractometer analyses showed relatively large discrepancies, indicating that the Fehling's method could also be effective for the analysis of fermentable sugars in the manufacture of Makgeolli.