• Title/Summary/Keyword: DNA barcode

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A Newly Recognized Desmometopa Loew, 1866 (Diptera: Milichiidae), A Commensalistic Dipteran Genus, in South Korea (한국의 미기록속 이마줄불청객파리속(신칭)(파리목: 불청객파리과)에 대한 보고)

  • Seung-Su Euo;Jeong-Hwan Choi;Il-Kwon Kim;A Young Kim
    • Korean journal of applied entomology
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    • v.62 no.4
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    • pp.325-331
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    • 2023
  • Desmometopa Loew, 1866 is newly recognized in the Korean Peninsula, based on Desmometopa microps Lamb, 1914 that is new to Korean insect fauna. The species is known to have commensalistic habits with predacious insects and spiders. Diagnosis, description, images and DNA barcode sequences are provided, based on the obtained adult specimens.

Are Cryptic Species Real?

  • Crous, Pedro W.
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.29-29
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    • 2014
  • Since Darwin and Wallace introduced the concept on the evolution of species, scientists have been furiously debating what species are, and how to define them. This basic yet intriguing question has bothered us ever since, as communicating to fellow biologists about fungal species is the very cornerstone of mycology. For the species presently known, this has largely been accomplished via Latin binomials linked to morphology in the absence of DNA barcodes. In recent years mycologists have embraced the ribosomal ITS as official barcode region for Fungi, and this locus is also mainly used in environmental pyrosequencing studies. Furthermore, DNA data can now also be used to describe sterile species in the absence or lack of distinct morphological structures. Recent developments such as the registration of names in MycoBank, and linking the phenotype to the genotype, have significantly changed the face of fungal systematics. By employing the Consolidated Species Concept, incorporating genealogical concordance, ecology and morphology, robust species recognition is now possible. Several international initiatives have since built on these developments, such as the DNA barcoding of holdings of Biological Resource Centres, followed by the Genera of Fungi Project, aiming to recollect, and epitypify all type species of all genera. What these data have revealed, is that most genera are poly- and paraphyletic, and that morphological species normally encompass several genetic entities, which may be cryptic species. Once we provide a stable genetic backbone capturing our existing knowledge of the past 250 years, we will be able to accommodate novelties obtained via environmental sequencing platforms. Being able to communicate these species to other biologists in a clear manner that is DNA-based, will enable scientists to elucidate the importance, role and ecological interactions that these fungi have on our planet.

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Genetic Analysis of Medicinal Plants in Adenophorae Radix Using DNA Barcode (DNA바코드를 활용한 사삼(沙蔘)의 종 감별)

  • Kim, Minkyeoung;Lee, Wookyu;Kim, Jaelim;Lee, Kiho;Choi, Yoorae;Kim, Jonghwan;Kang, Ilhyun;Kang, Juhye
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.97-97
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    • 2019
  • 사삼(沙蔘, Adenophorae Radix)은 "대한민국약전외한약(생약)규격집(KHP)"에 잔대 Adenophora triphylla var. japonica Hara 또는 사삼(당잔대, A. stricta Miq.)의 뿌리로 수재되어 있으나, 형태학적으로 유사한 제니(모시대, A. remotiflorus Miquel), 층층잔대(윤엽사삼, A. tetraphylla (Thunb.) Fisch), 더덕 Codonopsis lanceolata (Sieb. et Zucc.)과 오 혼용 우려가 있어 이들을 구별하기 위한 종 감별법이 필요하다. 본 연구에서는 '사삼'과 오 혼용 우려가 있는 종들을 구별할 수 있는 유전자 마커 개발을 위하여 DNA 바코드로 활용되고 있는 유전자 부위를 분석하여 ITS (25%), atpB-rbcL (15%), atpF-atpH (14%), rpl16 (13%), trnL-F (10%), matK (9%), rpoC1 (7%)에서 변이율(percent of variable sites)을 확인하였다. 또한, 분석한 유전자 부위 중 종간 차이를 확인하기 용이한 matK 구간을 활용해 기원종인 잔대, 당잔대와 형태적으로 유사하여 오 혼용될 우려가 있는 층층잔대, 모시대 및 더덕을 감별 할 수 있는 유전자 마커를 개발하였다. 본 연구를 통해 얻어진 염기서열과 분자 마커는 '사삼'의 품질관리에 유용하게 활용 가능할 것으로 사료된다.

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New record of two feather mites (Acari: Sarcoptiformes: Astigmata) from Korea

  • Han, Yeong-Deok;Song, Ji-Hun;Min, Gi-Sik
    • Journal of Species Research
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    • v.5 no.3
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    • pp.324-332
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    • 2016
  • Two feather mites, Zachvatkinia larica Mironov, 1989 and Ardeacarus ardeae (Canestrini, 1878) are reported for the first time in Korea. Specimens of Z. larica and A. ardeae were collected from the blacktailed gull Larus crassirostris and little egret Egretta garzetta, respectively. The genera Zachvatkinia Dubinin, 1949 and Ardeacarus Dubinin, 1951 are new to this country. Here, we provided morphological descriptions and illustrations of these two species. Additionally, we provide the partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) as DNA barcode markers.

First report of the branchiobdellidan Holtodrilus truncatus (Annelida: Clitellata) found on the freshwater atyid shrimp Neocaridina sp. from Korea

  • Ahn, Dong-Ha;Min, Gi-Sik
    • Journal of Species Research
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    • v.5 no.3
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    • pp.459-462
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    • 2016
  • The branchiobdellidan species Holtodrilus truncatus (Liang, 1963) is reported for the first time in Korea. The genus Holtodrilus Gelder and Brinkhurst, 1990 is monotypic within the family Xironodrilidae, and the genus and family of this species are also new to the Korean fauna. The branchiobdellidan specimens were collected from the exoskeletons of the freshwater atyid shrimp, Neocaridina sp., sampled from the 'Andeok Valley', Jeju-do Province, South Korea. Holtodrilus truncatus is characterized by its terete body form, 7/7 dental formula (with a large medial tooth and three pairs of smaller lateral teeth), and no trunk appendages. In addition, we determined a partial sequence of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene as a DNA barcode marker for H. truncatus. The present study is the first record of the branchiobdellidan occurrence on a non-crayfish host (shrimp) in Korea. To date, seven species (four genera in three families) of branchiobdellidans have been reported in the Korean fauna.

First Discovery of the Lichen-Feeding Moth Bacotia sakabei (Lepidoptera: Psychidae) from Korea

  • Roh, Seung Jin;Byun, Bong-Kyu
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.1
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    • pp.60-64
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    • 2017
  • The family Psychidae is a small group consisting of 241 genera and 1,350 species in the world. The genus Bacotia, belonging to the family Psychidae, what was established by Tutt based on the type species, Fumea sepium Speyer and Speyer, 1846. In this study, we aimed to record lichen-feeding bagworms in Korea. The genus Bacotia Tutt is reported for the first time from Korea with one newly recorded species, Bacotia sakabei Seino, 1981. All available information, including the collecting localities, host plants, illustrations of adults and genitalia are presented. A DNA barcode for precise identification of the species is also described.

New earthworm species from NIBR's Jeju-do biosphere compared to historical and new Japanese types (Oligochaeta: Megadrilacea: Megascolecidae)

  • Blakemore, Robert J.
    • Journal of Species Research
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    • v.1 no.2
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    • pp.133-150
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    • 2012
  • Amynthas tralfamadore sp. nov. is described from the Mt Halla, Jeju Island biosphere exhibition housed at NIBR Incheon. This species' taxonomy is problematical since it is similar to Amynthas masatakae (Beddard, 1892) from Japan, itself previously reported from Korea and at one time associated with Amynthas campestris (Goto & Hatai, 1898) and A. parvicystis (Goto & Hatai, 1899), all three variously implicated in prior A. robustus (Perrier, 1872) along with Amynthas aspergillum (Perrier, 1872). Based on reinspection of the London lectotype of A. masatakae-here designated and sketched for the first time-the current solution is for maintenance of all five taxa separately. A closely-related Japanese species-Metaphire ryunome sp. nov.-is comparable to Korean Metaphire reisuiensis (Kobayashi, 1938) comb. nov. Another specimen was identified as Amynthas micronarius (Goto & Hatai, 1898), a new record for Korea. It matches the newly-designated neotype (Tokyo NMST An446) and an annotated synonymy is provided; however, erstwhile synonyms, Amynthas shimaensis (Goto & Hatai, 1899) and A. yamizoyamensis (Ohfuchi, 1935) combs. novae, are briefly redescribed and provisionally restored to the Japanese list. mtDNA COI-5P barcode analyses support species identifications.

A report on species of phyllidiid and polycerid nudibranch including two species new to Korea

  • Jung, Daewui;Lee, Jongrak;Kim, Chang-Bae
    • Journal of Species Research
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    • v.2 no.1
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    • pp.7-14
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    • 2013
  • During a systematic study on Korean nudibranchs, two phyllidiid and two polycerid species were collected and identified with examination of their external morphological characters. As a result of the study, two phyllidiid species, Phyllidia ocellata Cuvier, 1804 and Phyllidiella pustulosa (Cuvier, 1804) were redescribed since there was no descriptions of these species with Korean specimens. And two polycerid species, Thecacera pennigera (Montagu, 1815) and Triopha catalinae (Cooper, 1863) were described as new to Korean fauna. Diagnostic characters of these species are described with illustrations in this study. DNA barcode data from three species examined were also provided for a supplement to morphological identifications.

First Record of Indo-West Pacific Spider Crab, Naxioides robillardi (Decapoda: Epialtidae) from Korean Waters

  • Lee, Sang-Hui;Jung, Jibom;Park, Taeseo
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.4
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    • pp.400-407
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    • 2020
  • An epialtid spider crab, Naxioides robillardi (Miers, 1882), is known as widely distributed in Indo-West Pacific region include Japanese waters. Four specimens of epialtid crabs were collected from adjacent waters of Jejudo Island and identified as N. robillardi. This species can be grouped into two forms such as N. robillardi form typical and N. robillardi form mammillata according to morphological features of carapace, gastric spine, and chela. Morphological characters of all Korean specimens well agree with the form mammillata in general. In this paper, morphological diagnosis and illustrations of newly collected N. robillardi are provided. DNA barcode sequence of COI region is also determined for the first time.

The First Record of Leocratides kimuraorum (Annelida, Hesionidae) from Korea, with DNA Barcode Data

  • Kim, Hana;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.37 no.3
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    • pp.219-224
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    • 2021
  • A hesionid species, Leocratides kimuraorum Jimi, Tanaka and Kajihara, 2017 is newly reported from the sublittoral zones (100 m depth) of the Korean coasts. This species is characterized by lateral antennae as long as the palps, peristomial membrane without papillose, peristomial dorsolateral tubercles with two round marginal lobes, and pharyngeal with terminal papillae. The intra-specific genetic distance among the cytochrome c oxidase subunit I(COI) sequences of L. kimuraorum specimens from Japan (type locality) and Korea (this study) was in the range of 0.002-0.005. The inter-specific genetic distance between L. kimuraorum and other hesionid species were 0.166-0.307. The present study is the first record of Leocratides species in Korean fauna. This paper also provides a morphological description and photographs of L. kimuraorum, with partial sequences of the mitochondrial COI based on Korean specimens.