• Preventive Nutrition and Food Science
• /
• v.3 no.2
• /
• pp.198-202
• /
• 1998

The arbitrary primed polymerase chain reaction (AP-PCR) has been used to detect the genetic alternations in the related species. Simple and reproducible fingerprints of complex genomes can be generated using single arbitrary chosen primers and the PCR. The technique was applied to the Oryza species and characterized the relationship among three cultivars of rice species based on theresult of genomic DNA fingerprints. The results indicated that the polymorphism revealed in rice strains and the differences in the PCR product pattern could be represented for each strainis. There was many variationsin the PCR product pattern between cv. Dongin(japonica type)and cv.Hyangdo (indica type), and our chosen AP-primers can ge as markers for strain identification and verfication.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.3
• /
• pp.315-319
• /
• 2003

Random amplified polymorphic DNA (RAPD) analysis was done with 19 oligonucleotide primers to study genetic similarities and divergence among different types of Deoni breed of cattle viz., Balankya, Wannera and Waghya. Six random primers produced low to high numbers of polymorphic bands between pooled DNA of different Deoni types. Of the 48 RAPD markers obtained 33 were common to all Deoni types, 3 were individual specific and 12 were polymorphic for different Deoni types. The mean average percentage difference values among Deoni types showed that Balankya and Wannera had less genetic divergence when compared to Waghya.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.29 no.6
• /
• pp.761-769
• /
• 1996

The random amplified polymorphic DNA (RAPD) technique was used to characterize 18 reference strains of microalgae, mostly Chlorella species, collected from various localities around Korea peninsular. Eighteen strains consist of four genera of the family marine Chlorella from 12 samples, two genera of fresh water Chlorella from three samples, and three genera on Nannochloris. Twenty 10-mer anonymous primers were screened for amplification of genomic DNA extracted from samples using the CTAB extraction method. Nineteen of these oligonucleotide primers were positive or band producing. Three of 20 random primers (OPA 10, OPA 12, and OPA 18) resulted in both clear band and a high degree of reproducibility and showed some potential to be used to discriminate individual samples of both genetically hetero-and homogeneous populations, in determining phylogenetic relationships between species within a genus and developing individual fingerprints for each samples.

• Journal of KIISE:Software and Applications
• /
• v.26 no.11
• /
• pp.1350-1358
• /
• 1999

이 논문에서는 유전밴드 영상신호에 포함되어 있는 지형적 특징을 이용하여 밝기의 변화가 일정하지 않은 유전밴드를 인식하는 방법을 연구하였다. 유전밴드는 동일인을 식별하는데 있어서 지문보다 높은 신뢰성을 가지고 있으므로, 유전밴드 영상에서 유전밴드의 유무와 위치를 자동적으로 검출하는 것은 매우 중요하다. 레인내의 밝기의 변화가 일정한 유전밴드는 미분연산자에 의해 검출할 수 있지만, 밝기의 변화가 일정하지 않은 레인내의 유전밴드는 일반적인 인식방법에 의해서는 검출하기 어렵다. 따라서 유전밴드 영상으로부터 지형적 특징을 추출하고, 이것으로부터 계산한 곡률(curvature)의 크기에 의해 유전밴드를 인식함으로써 레인의 밝기가 변화하는 경우에도 효과적으로 인식하였다.Abstract This paper presents recognition of DNA band using the topographical features of DNA band images. The DNA band provides a more reliable way of identification than fingerprints. Recognition based on differentiation operators can easily detect the DNA band if the brightness of lane in the image is almost uniform. When the brightness of the lane changes gradually, the DNA bands are hard to be recognized. Using the curvature magnitude of the lane computed from topographic features extracted from DNA images, the DNA bands are efficiently recognized in the lane whose brightness changes.

• Korean Journal Plant Pathology
• /
• v.14 no.2
• /
• pp.164-170
• /
• 1998

This study was carried out to develop DNA probe for specific detection of Erwinia carotovora subsp. carotovora. Universal rice primer (URP, 20 mer) developed from repetitive sequence of rice was applied for producing PCR DNA fingerprints of Erwinis spp. In E. carotovora subsp. carotovora strains, primer URP2F amplyfied polymorphic bands which are distinguisable from other Erwinia spp. A PCR band of 0.6 kb selected from PCr polymorphic bands of E. carotovora subsp. carotovora strains was cloned and evaluated as a diagnostic DNA probe. Among 28 bacterial strains including 22 Erwinia spp, the probe (pECC2F) only hybridized to total DNAs from e. carotovora subsp. carotovora strains and E. carotovora subsp. wasabiae, but sizes of hybridized bands were different between these subspecies, 10.0 kb and 3.5 kb respectively. In dot blot assays using probe pECC2F, as few as 103 colony forming units (CFU) of E. carotovora subsp. carotovora could be detected in a suspension containing about 1$\times$103 CFU of soil bacteria.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.3
• /
• pp.309-314
• /
• 2002

Random Amplified Polymorphic DNA (RAPD) assay was conducted to identify polymorphic markers in Amrithmahal, Krishna Valley, Hallikar, Deoni, Khillari, Ongole and Malnad Gidda breeds of South Indian cattle using twenty six primers. Of the 93 RAPD markers obtained, 53 were present in all breeds, 22 were individual specific and 18 were polymorphic for different breeds. Dual purpose breeds viz., Krishna Valley and Ongole showed less genetic divergence between them as compared to their genetic divergence from draft breeds viz., Amrithmahal, Hallikar and Khillari. Malnad Gidda was found to be a distinctly different from others studied.

• Analytical Science and Technology
• /
• v.29 no.2
• /
• pp.85-93
• /
• 2016

This study investigated the possibility of DNA contamination during fingerprint collection when using a fingerprint brush. Two kinds of brushes were selected: powdered brushes and neat (not powdered) brushes. The fingerprints were collected from the tips of all the fingers and near the wrists of both living and deceased persons using the two brushes. Both brushes were analyzed for the DNA contents and profiles. The results obtained confirmed the transfer of DNA onto both brushes, although the results showed that the powdered brushes carried more DNA compared with the neat brushes. More DNA was transferred onto the brushes used on deceased persons than onto the brushes used for living persons. Only partial DNA profiles were obtained from the brushes, which is due to the presence of other sources of DNA on the surfaces of the skin of both living and deceased persons. This phenomenon confirmed the DNA contamination during fingerprint collection when fingerprint brushes were used.

• Fisheries and Aquatic Sciences
• /
• v.17 no.4
• /
• pp.461-469
• /
• 2014

Polychlorinated biphenyls (PCBs) are persistent pollutants in aquatic environments, often causing the decline or disappearance of wild populations. In this study, we used a random amplified polymorphic DNA (RAPD) assay to evaluate the effects on the genomic DNA of olive flounder embryo and larval stages of exposure to Aroclor 1254 at concentrations of 1, 5, 10, 20, and $40{\mu}g/L$. We compared RAPD fingerprints of exposed and non-exposed samples. Polymorphisms were revealed as the presence and/or absence of DNA fragments between the two samples. A dose-dependent increase in the number of polymorphic bands was observed with Aroclor 1254 treatment. Also, RAPD profiles of animals exposed to Aroclor 1254 exhibited an increase in the frequency values (FV) compared to the control. A phenogram constructed using neighbor-joining method indicated that genomic template stability in developing embryo and larval stages was significantly affected at ${\geq}5{\mu}g/L$. This study suggested that DNA polymorphisms detected by RAPD analysis could be used as an investigative tool for environmental toxicology and as a useful biomarker in early life stages for the detection of potential genotoxicants.

• The Journal of the Korea Contents Association
• /
• v.19 no.1
• /
• pp.463-471
• /
• 2019

Tapes are usually used in violent crimes and may contain important evidence. Separating adhesive side should be preceded to collect these evidence. In this study, we researched a novel dual-purpose reagent that can separate the tape without damaging and develop the fingerprints on porous surface when the tape is attached to A4 paper. As a result, the reagent of 1:2 ratio of 1,2-IND stock solution and HFE-7100 can separate without damaging the adhesive surface and develop fingerprints on separated A4 paper.

• Horticultural Science & Technology
• /
• v.30 no.5
• /
• pp.549-556
• /
• 2012

The study aimed to establish an efficient tool for cultivar identification and characterization being the first steps of apple introduction and improvement program. We utilized a method to efficiently record DNA molecular fingerprints of plant individuals genotyped by RAPD, which could be used as efficient reference information for quick plant identification. Ten of sixty 11-mer primers were screened to identify the 68 apple genotypes which could be distinguished by a combination of several primers. All cultivars were easily identified by the corresponding primers marked on the cultivar identification diagram (CID). The results indicated that the CID strategy developed and employed in the apple cultivar identification could be vital in the utilization of DNA marker in other plants as well as the development of the apple industry.