• Animal Systematics, Evolution and Diversity
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• v.35 no.3
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• pp.156-159
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• 2019

The phyllodicd polychaete species, Nereiphylla hera Kato and Mawatari, 1999 is reported from the intertidal habitats of the eastern coast of South Korea. We determined the DNA barcoding region of the mitochondrial cytochrome c oxidase subunit I (COI) of N. hera and compared nucleotide variation with its congeners. The intra-specific genetic distance between the three COI sequences of N. hera was ranged from 0 to 0.4%. The inter-specific distances between N. hera and other Nereiphylla species ranged from 18.8 to 22.3%. In this study, we reported the first COI barcodes of N. hera with the morphologcial diagnosis and the photographs. These results would be helpful to understand taxonomy of Nereiphylla.

• Animal Systematics, Evolution and Diversity
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• v.38 no.2
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• pp.108-112
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• 2022

An aegid species, Rocinela niponia Richardson, 1909, is a Far Eastern species known from Korean and Japanese waters. In this study, mitochondrial cytochrome c oxidase subunit I (COI) sequences of R. niponia were determined based on four specimens collected from the subtidal zone of Chujado Island, South Korea. We compared DNA barcoding data of this species with its congeners. As a result, there was no intra-specific genetic distance between the four COI sequences of R. niponia. Inter-specific distances between R. niponia and other five aegid species ranged from 23.8% to 35.6%. Morphological diagnosis and images of R. niponia are also provided as a valuable contribution toward the identification of Rocinela species in further taxonomic and ecological studies.

• Animal Systematics, Evolution and Diversity
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• v.39 no.3
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• pp.118-122
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• 2023

The genus Hydroporus Clairville is the most diverse group belonging to the subfamily Hydroporinae in the family Dytiscidae. However, members of this group have not been recorded in the Korean peninsula. The genus Hydroporus Clairville is characterized by the following features: body glabrous to distinctly setose; elytra and ventral surface mainly piceous to black; epipleuron lacking a carina on humeral angle; metacoxal process conjointly with hind margin straight or slightly angulate medially. We collected the high mountain wetland and identified the species of Hydroporus uenoi Nakane based on male genitalia for the first time. A habitus photo, a redescription, mitochondrial DNA sequence, illustrations of the aedeagus of species, and habitat information are provided.

• Molecules and Cells
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• v.23 no.2
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• pp.220-227
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• 2007

The development of suitable genetic markers would be useful for defining species and delineating the species boundaries of morphologically indistinguishable sponges. In this study, genetic variation in the sequences of nuclear rDNA and the mitochondrial cytochrome c oxidase subunit 1 and 3 (CO1 and CO3) regions were compared in morphologically indistinguishable Korean Halichondriidae sponges in order to determine the most suitable species-specific molecular marker region. The maximal congeneric nucleotide divergences of Halichondriidae sponges in CO1 and CO3 are similar to those found among anthozoan cnidarians, but they are 2- to 8-fold lower than those found among genera of other triploblastic metazoans. Ribosomal internal transcribed spacer regions (ITS: ITS1 + ITS2) showed higher congeneric variation (17.28% in ITS1 and 10.29% in ITS2) than those of CO1 and CO3. Use of the guidelines for species thresholds suggested in the recent literature indicates that the mtDNA regions are not appropriate for use as species-specific DNA markers for the Halichondriidae sponges, whereas the rDNA ITS regions are suitable because ITS exhibits a low level of intraspecific variation and a relatively high level of interspecific variation. In addition, to test the reliability of the ITS regions for identifying Halichondriidae sponges by PCR, a species-specific multiplex PCR primer set was developed.

• Journal of Species Research
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• v.1 no.1
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• pp.35-43
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• 2012

Japanese/Korean Amynthas carnosus (Goto & Hatai, 1899) is redescribed from a newly designated Neotype (Tokyo NSMT An435). An annotated synonymy is presented that nominally includes: kyamikia Kobayashi, 1934, monstrifera Kobayashi, 1936, sangyeoli, youngtai, kimhaeiensis, sinsiensis and baemsagolensis - all Korean names by Hong & James, 2001, and Taiwanese monsoonus James et al., 2005. However, Chinese A. pingi (Stephenson 1925) (syn. fornicata Gates, 1935) is maintained separately, at least for the present, until its comprehensive review, possibly extending to DNA barcode differentiation.

• Animal Systematics, Evolution and Diversity
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• v.40 no.4
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• pp.332-339
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• 2024

For the first time in Korea, we report two species from the family Noctuidae: Trichosea ainu (Wileman), and Virgo confusa Kishida and Yoshimoto, as well as two species from the family Erebidae: Paracolax bilineata (Wileman) and Homodes vivida Guenée. Most of these species were collected from southern regions of Korea, including Wando, Gangjin, Shinan, and Jejudo. We provide photographs of adults and male and female genitalia, along with mitochondrial DNA(COI barcode region) sequences.

• Journal of Food Hygiene and Safety
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• v.35 no.3
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• pp.234-242
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• 2020

In this study, we monitored the raw materials in home-meal replacement (HMR) products, which have shown more than 63% growth in market size for two years. A total of 89 HMR products were purchased and the DNA barcodes of 112 raw materials in the product samples were analyzed. In order to identify the raw material species, a primer set specific for the 16S ribosomal RNA region of each raw material species was amplified. The amplicon was purified and sequenced, and then used to perform a BLAST search provided by the National Institutes of Health (NIH). The species of the raw material was determined by comparing the nucleotide sequences of the species registered in GenBank with identity and match score. Twenty-four species and three genera were identified from 112 raw materials. Three genera were identified at the genus level because a large number of species belonging to the same genus exist within 98% of the identity criteria. The results of the determination were compared with the available raw materials suggested in the Korea Food Code to determine the Korean name and availability of the foods. Six non-listed species were determined to be edible according to information provided by influential domestic and foreign organizations.

• Korean Journal of Plant Taxonomy
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• v.50 no.3
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• pp.318-326
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• 2020

We found an unrecorded species, Carex molestiformis Reznicek & Rothrock, in Byeokparyeong, Daehwa-myeon, Pyeongchang-gun, Gangwon-do, South Korea. This species is native to southern parts of the United States (ca. 11 states, including Arkansas, Missouri, and Oklahoma). These plants are usually distributed densely in stream flood plains, bottomlands, stream banks, and along roadsides. Belonging to section Ovales Kunth, perennial herbs with gynecandrous spikes, C. molestiformis is closely related to C. maackii Maxim. and C. scoparia Willd. and is distinguished from them by the number (2-4) of spikes in each inflorescence and the width (2.63.4 mm) and number (69) of veins of perigynia. A new Korean name of C. molestiformis 'Ga-Neun-Ta-Rae-Sa-Cho' was given, as it has slender overall appearance compared with C. maackii (Ta-Rae-Sa-Cho). We also provide a description, illustrations, photographs and a key of related taxa in Korea. We compared three DNA barcode region (chloroplast DNA matK, ndhF and nuclear ribosomal DNA internal transcribed spacer) sequences from C. molestiformis with those of C. maackii and C. scoparia, determining eight species-specific single nucleotide polymorphism sites for C. molestiformis.

• Animal Systematics, Evolution and Diversity
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• v.40 no.2
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• pp.130-134
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• 2024

The family Velutinidae is found in various intertidal and subtidal habitats worldwide including Arctic and Antarctic seas. They are characterized by possessing a fragile shell that is partially or entirely covered by the mantle. Eight valid species of the genus Coriocella have been reported mostly in the Indo-West Pacific. Here we report Coriocella jayi Wellens, 1996 from Korean waters for the first time and describe details of their external morphology and radula characteristics using scanning electron microscopy, and provide the mtDNA cox1 sequence as a DNA barcode sequence information. This species is distinguished from other congeneric species by having six cylinder-shaped tubercular lobes of their dorsal part of mantle body and mantle color. Phylogenetic tree using the mtDNA cox1 sequence data shows that two Coriocella species (C. jayi and C. nigra) are grouped as their respective sister among Velutinidae species, and these relationships are strongly supported by 100% bootstrap value. Despite the morphological similarities, further investigation will be needed to confirm whether the African and Korean populations can be justified as the same species with a disconnected distribution range, or represent morphologically similar but two distinct species.

• Animal Systematics, Evolution and Diversity
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• v.40 no.4
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• pp.347-353
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• 2024

The family Lottiidae Gray, 1840, belonging to the subclass Patellogastropoda (commonly known as "true limpets"), comprises approximately 200 species worldwide. In Korean waters, 20 species of Lottiidae have been sparsely recorded to date, including eight species from the genus Lottia Gray, 1833. Lottia species typically exhibit significant ecophenotypic shell variations, which have led to various taxonomic complications, including the proliferation of synonyms due to species misidentification. In this study, we provide detailed information of the shell and radula morphologies using scanning electron microscopy, coupled with mtDNA cox1 sequences as DNA barcode references for L. peitaihoensis (Grabau & S. G. King, 1928), newly discovered in Korean waters. Morphologically, L. peitaihoensis can be distinguished from other congeneric species by its shell, characterized by having numerous fine radial riblets with even finer riblets intercalated among them. The radula of this species is characterized by short, pointed first lateral teeth (innermost-lateral teeth), long, slightly rounded second lateral teeth (outermost-lateral teeth), and long, broad, beak-like third lateral teeth (outermost-lateral teeth). In addition, we reconstructed a phylogenetic tree using 25 mtDNA cox1 haplotype sequences from five Lottia species deposited in GenBank, including two newly obtained sequences from Korean L. peitaihoensis samples to confirm the species. The detailed morphological data, coupled with molecular information presented in this study, provide valuable information for the accurate delineation and species identification of closely related species within the genus Lottia.