• Parasites, Hosts and Diseases
• /
• 제59권2호
• /
• pp.173-178
• /
• 2021

The DM9 domain is a protein unit of 60-75 amino acids that has been first detected in the fruit fly Drosophila as a repeated motif of unknown function. Recent research on proteins carrying DM9 domains in the mosquito Anopheles gambiae and the oyster Crassostrea gigas indicated an association with the uptake of microbial organisms. Likewise, in the trematode Fasciola gigantica DM9-1 showed intracellular relocalization following microbial, heat and drug stress. In the present research, we show that FgDM9-1 is a lectin with a novel mannose-binding site that has been recently described for the protein CGL1 of Crassostrea gigas. This property allowed FgDM9-1 to agglutinate gram-positive and -negative bacteria with appropriate cell surface glycosylation patterns. Furthermore, FgDM9-1 caused hemagglutination across all ABO blood group phenotypes. It is speculated that the parenchymal located FgDM9-1 has a role in cellular processes that involve the transport of mannose-carrying molecules in the parenchymal cells of the parasite.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
• /
• pp.64-64
• /
• 2003

The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the $^{32}$ P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.

• 지능정보연구
• /
• 제9권2호
• /
• pp.19-38
• /
• 2003

In this research, we propose the mechanism to develop self-evolving expert systems (SEES) based on data mining (DM), fuzzy neural networks (FNN), and relational database (RDB)-driven forward/backward inference engine. Most researchers had tried to develop a text-oriented knowledge base (KB) and inference engine (IE). However, this approach had some limitations such as 1) automatic rule extraction, 2) manipulation of ambiguousness in knowledge, 3) expandability of knowledge base, and 4) speed of inference. To overcome these limitations, knowledge engineers had tried to develop an automatic knowledge extraction mechanism. As a result, the adaptability of the expert systems was improved. Nonetheless, they didn't suggest a hybrid and generalized solution to develop self-evolving expert systems. To this purpose, we propose an automatic knowledge acquisition and composite inference mechanism based on DM, FNN, and RDB-driven inference engine. Our proposed mechanism has five advantages. First, it can extract and reduce the specific domain knowledge from incomplete database by using data mining technology. Second, our proposed mechanism can manipulate the ambiguousness in knowledge by using fuzzy membership functions. Third, it can construct the relational knowledge base and expand the knowledge base unlimitedly with RDBMS (relational database management systems) module. Fourth, our proposed hybrid data mining mechanism can reflect both association rule-based logical inference and complicate fuzzy relationships. Fifth, RDB-driven forward and backward inference time is shorter than the traditional text-oriented inference time.

• Tuberculosis and Respiratory Diseases
• /
• 제60권2호
• /
• pp.187-193
• /
• 2006

배 경 : 결핵균의 성장 속도가 늦은 이유가 mammalian cell이나 대장균에 비해 결핵균 AK의 매우 낮은 활성도에 의한 것이라는 추측으로부터 AK1과 유사한 촉매능을 나타내는 AKmt 돌연변이 유전자와 human muscle-type AK 합성 유전자(AK1)를 각각 Mycobacterium/E.coli 발현벡터에 재조합(pMVAKmtDM, pEMAK1)하여 성장 속도가 매우 느린 BCG에 형질전환함으로써 이들 단백질들의 촉매능에 의한 성장 속도 변화가 일어나는지를 확인하고자 하였다. 방 법 : Human AK1의 촉매 활성도와 유사하도록 결핵균 AK (AKmt)유전자의 ATPbd와 LID domain을 돌연변이하여 제조한 유전자(AKmtDM)와 human muscle-type adenylate kinase 합성 유전자(AK1)를 Mycobacterium/E.coli 발현벡터에 클로닝하여 재조합 BCG를 제조하였고, 이들 재조합 BCG와 BCG Pasteur $1173P_2$ (wild-type)를 7H9 액체배지에 접종하여 2-3 일 간격으로 $A_{600}$ 값을 측정하였다. 결 과 : 재조합 BCG의 성장 속도는 Wild-type BCG의 성장 속도에 비해 변화가 없었다. 결 론 : 결핵균 adenylate kinase의 정확한 기능은 알 수 없으나, adenylate kinase의 촉매 활성도의 증가는 BCG의 성장 속도에는 영향을 주지 않는 것으로 판단된다.

• 자원환경지질
• /
• 제27권3호
• /
• pp.263-279
• /
• 1994

Paleomagnetic and rock-magnetic data of Cretaceous sedimentary and volcanic rocks from the Euiseong area indicate that the stable components of remanence are carried by single and pseudo-single domain magnetite, with the exception of the Shinyangdong Formation which has been remagnetized. The Hayang Group, except for the remagnetized Shinyangdong Formation, yields the mean characteristic direction of $D/I=22.5^{\circ}/57.2^{\circ}$ (${\alpha}_{95}=4.6^{\circ}$, N=14 sites) and the pole position is $72.0^{\circ}N$, $206.4^{\circ}E$ ($dp/dm=4.9^{\circ}/6.7^{\circ}$). The Yucheon Group shows two polarities and the mean characteristic direction of $D/I=351.2^{\circ}/60.5^{\circ}$ (${\alpha}_{95}=11.2^{\circ}$, N= 19 sites) and the pole position is $81.3^{\circ}N$, $79.0^{\circ}E$ ($dp/dm=13.0^{\circ}/17.0^{\circ}$). The mean directions of both the Hayang and the Yucheon Groups are supported by the McElhinny's fold test at the 99% confidence level and that of the Yucheon Group by a reversal test at the 95% confidence level. A magnetostratigraphic correlation between polarities of the study formations and the Geomagnetic Time Scale indicates that the Hayang Group can be correlated to the Cretaceous Long Normal Superchron (CLNS), and the Yucheon Group to the boundary between the CLNS and the Polarity Chron 33R or later boundaries between normal and reverse polarities. Comparison of the paleopoles from this study with those from the surrounding areas both within the Gyeongsang basin and in the northeastern Asia indicates that the study area was not undergone significant tectonic rotations with respect to the other parts of the Gyeongsang basin and that the Korean Peninsula was the part of the single terrane of the northeastern Asia at least since the CLNS. The Yucheon Group can be divided into four sub-groups based on the paleomagnetic data, suggesting that there were at least four times of volcanic activities in the study area.